LOCUS       Exported                1682 bp DNA     linear   UNA 01-SEP-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 17, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             90..134
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /note="color: #993366
                     Cleavage site after base 134"
                     /translation="MSALLILALVGAAVA"
     CDS             135..158
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     CDS             171..509
                     /codon_start=1
                     /label=Fzd3
                     /note="color: #339966"
                     /translation="HSLFSCEPITLRMCQDLPYNTTFMPNLLNHYDQQTAALAMEPFHP
                     MVNLDCSRDFRPFLCELYAPICMEYGRVTLPCRRLCQRAYSECSKLMEMFGVPWPEDME
                     CSRFPDCDE"
     CDS             510..1640
                     /codon_start=1
                     /label=Fzd4
                     /note="color: #993366"
                     /translation="GPGDEEVPLPHKTPIQPGEECHSVGSNSDQYIWVKRSLNCVLKCG
                     YDAGLYSRSAKEFTDIWMAVWASLCFISTTFTVLTFLIDSSRFSYPERPIIFLSMCYNI
                     YSIAYIVRLTVGRERISCDFEEAAEPVLIQEGLKNTGCAIIFLLMYFFGMASSIWWVIL
                     TLTWFLAAGLKWGHEAIEMHSSYFHIAAWAIPAVKTIVILIMRLVDADELTGLCYVGNQ
                     NLDALTGFVVAPLFTYLVIGTLFIAAGLVALFKIRSNLQKDGTKTDKLERLMVKIGVFS
                     VLYTVPATCVIACYFYEISNWALFRYSADDSNMAVEMLKIFMSLLVGITSGMWIWSAKT
                     LHTWQKCSNRLVNSGKVKREKRGNGWVKPGKGNETVV"
     CDS             1650..1667
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
ORIGIN
        1 tactatgaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
       61 cccaagctgg ctagttaagc ttggtaccaa tgtctgcact tctgatccta gctcttgttg
      121 gagctgcagt tgctgactac aaagacgatg acgacaagct tgcggccgca cacagtttgt
      181 tttcttgtga acctattacc ttgaggatgt gccaagattt gccttataat actaccttca
      241 tgcctaatct tctgaatcat tatgaccaac agacagcagc tttggcaatg gagccattcc
      301 accctatggt gaatctggat tgttctcggg atttccggcc ttttctttgt gaactctacg
      361 ctcctatttg tatggaatat ggacgtgtca cacttccctg tcgtaggctg tgtcagcggg
      421 cttacagtga gtgttcgaag ctcatggaga tgtttggtgt tccttggcct gaagatatgg
      481 aatgcagtag gttcccagat tgtgatgagg gaccaggtga tgaagaggtt cccttgcccc
      541 acaagactcc catccagccc ggggaagagt gccactccgt gggaagcaat tctgatcagt
      601 acatctgggt gaagaggagc ctgaactgtg ttctcaagtg tggctacgat gctggcttgt
      661 acagccgctc agctaaggag ttcacggata tttggatggc tgtgtgggcc agcctctgct
      721 tcatctccac caccttcacc gtgctgacct tcctgattga ttcatccagg ttttcttacc
      781 ctgagcgccc catcatattt ctcagtatgt gctataatat ttatagcatt gcttatattg
      841 ttcggctgac tgtaggccgg gaaaggatat cctgtgattt tgaagaggcg gcagagcccg
      901 ttctcatcca agaaggactt aagaacacag gatgtgcaat aattttcttg ctgatgtact
      961 tttttggaat ggccagctcc atttggtggg ttattctgac actcacttgg tttttggcag
     1021 ccggactcaa gtggggtcat gaagccattg aaatgcacag ttcttatttc cacatcgcag
     1081 cctgggctat tcccgcagtg aaaaccattg tcatcttgat tatgagacta gtggatgccg
     1141 atgaactgac tggcttgtgc tatgttggga accaaaacct agatgccctc actggctttg
     1201 tggtggctcc tctctttacg tatttggtga ttggaacgct gttcattgcg gcgggtttgg
     1261 tggccttatt caaaattcgg tccaatcttc aaaaagacgg gacaaagaca gacaagttgg
     1321 aaaggctaat ggtcaagatc ggggtcttct cagtactgta cacggttcct gcaacctgtg
     1381 tgattgcctg ttatttctat gaaatctcaa actgggcact ctttcgatat tctgcagatg
     1441 actcaaacat ggcagttgaa atgttgaaaa tttttatgtc tttgctcgtg ggcatcactt
     1501 caggcatgtg gatttggtct gccaaaactc ttcacacgtg gcaaaagtgt tctaaccgat
     1561 tggtgaattc tgggaaggta aagagagaga agagggggaa tggttgggtg aagccaggaa
     1621 aaggcaacga gactgtggta tagctcgagc atcatcacca tcaccatgag tagcccgagg
     1681 gt
//
