LOCUS       Exported                1712 bp DNA     linear   UNA 01-SEP-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 17, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        39..57
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             91..135
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /note="color: #993366
                     Cleavage site after base 135"
                     /translation="MSALLILALVGAAVA"
     CDS             136..159
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     CDS             172..540
                     /codon_start=1
                     /label=Fzd5
                     /note="color: #33cccc"
                     /translation="ASKAPVCQEITVPMCRGIGYNLTHMPNQFNHDTQDEAGLEVHQFW
                     PLVEIQCSPDLRFFLCSMYTPICLPDYHKPLPPCRSVCERAKAGCSPLMRQYGFAWPER
                     MSCDRLPVLGRDAEVLCMD"
     CDS             541..1671
                     /codon_start=1
                     /label=Fzd4
                     /note="color: #993366"
                     /translation="GPGDEEVPLPHKTPIQPGEECHSVGSNSDQYIWVKRSLNCVLKCG
                     YDAGLYSRSAKEFTDIWMAVWASLCFISTTFTVLTFLIDSSRFSYPERPIIFLSMCYNI
                     YSIAYIVRLTVGRERISCDFEEAAEPVLIQEGLKNTGCAIIFLLMYFFGMASSIWWVIL
                     TLTWFLAAGLKWGHEAIEMHSSYFHIAAWAIPAVKTIVILIMRLVDADELTGLCYVGNQ
                     NLDALTGFVVAPLFTYLVIGTLFIAAGLVALFKIRSNLQKDGTKTDKLERLMVKIGVFS
                     VLYTVPATCVIACYFYEISNWALFRYSADDSNMAVEMLKIFMSLLVGITSGMWIWSAKT
                     LHTWQKCSNRLVNSGKVKREKRGNGWVKPGKGNETVV"
     CDS             1681..1698
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
ORIGIN
        1 aactaagagg aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
       61 acccaagctg gctagttaag cttggtacca atgtctgcac ttctgatcct agctcttgtt
      121 ggagctgcag ttgctgacta caaagacgat gacgacaagc ttgcggccgc agcgtccaag
      181 gccccggtgt gccaggaaat cacggtgccc atgtgccgcg gcatcggcta caacctgacg
      241 cacatgccca accagttcaa ccacgacacg caggacgagg cgggcctgga ggtgcaccag
      301 ttctggccgc tggtggagat ccaatgctcg ccggacctgc gcttcttcct atgctctatg
      361 tacacgccca tctgtctgcc cgactaccac aagccgctgc cgccctgccg ctcggtgtgc
      421 gagcgcgcca aggccggctg ctcgccgctg atgcgccagt acggcttcgc ctggcccgag
      481 cgcatgagct gcgaccgcct cccggtgctg ggccgcgacg ccgaggtcct ctgcatggat
      541 ggaccaggtg atgaagaggt tcccttgccc cacaagactc ccatccagcc cggggaagag
      601 tgccactccg tgggaagcaa ttctgatcag tacatctggg tgaagaggag cctgaactgt
      661 gttctcaagt gtggctacga tgctggcttg tacagccgct cagctaagga gttcacggat
      721 atttggatgg ctgtgtgggc cagcctctgc ttcatctcca ccaccttcac cgtgctgacc
      781 ttcctgattg attcatccag gttttcttac cctgagcgcc ccatcatatt tctcagtatg
      841 tgctataata tttatagcat tgcttatatt gttcggctga ctgtaggccg ggaaaggata
      901 tcctgtgatt ttgaagaggc ggcagagccc gttctcatcc aagaaggact taagaacaca
      961 ggatgtgcaa taattttctt gctgatgtac ttttttggaa tggccagctc catttggtgg
     1021 gttattctga cactcacttg gtttttggca gccggactca agtggggtca tgaagccatt
     1081 gaaatgcaca gttcttattt ccacatcgca gcctgggcta ttcccgcagt gaaaaccatt
     1141 gtcatcttga ttatgagact agtggatgcc gatgaactga ctggcttgtg ctatgttggg
     1201 aaccaaaacc tagatgccct cactggcttt gtggtggctc ctctctttac gtatttggtg
     1261 attggaacgc tgttcattgc ggcgggtttg gtggccttat tcaaaattcg gtccaatctt
     1321 caaaaagacg ggacaaagac agacaagttg gaaaggctaa tggtcaagat cggggtcttc
     1381 tcagtactgt acacggttcc tgcaacctgt gtgattgcct gttatttcta tgaaatctca
     1441 aactgggcac tctttcgata ttctgcagat gactcaaaca tggcagttga aatgttgaaa
     1501 atttttatgt ctttgctcgt gggcatcact tcaggcatgt ggatttggtc tgccaaaact
     1561 cttcacacgt ggcaaaagtg ttctaaccga ttggtgaatt ctgggaaggt aaagagagag
     1621 aagaggggga atggttgggt gaagccagga aaaggcaacg agactgtggt atagctcgag
     1681 catcatcacc atcaccatga gtagccccag cc
//
