LOCUS       Exported                1566 bp DNA     linear   UNA 16-AUG-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 17, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1566
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..51
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /note="color: #993366
                     Cleavage site after base 51"
                     /translation="MSALLILALVGAAVA"
     CDS             52..75
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     primer_bind     79..110
                     /label=hFz6_19f_Not
                     /note="color: black; sequence: 
                     GATCGCGGCCGCACACAGTCTCTTCACCTGTGAACC"
     CDS             88..1557
                     /codon_start=1
                     /label=Feature 3
                     /note="This feature has 2 segments:
                        1:   88 ..  426 / #00ccff / hFz6
                        2:  427 .. 1557 / #993366 / mFz4"
                     /translation="HSLFTCEPITVPRCMKMAYNMTFFPNLMGHYDQSIAAVEMEHFLP
                     LANLECSPNIETFLCKAFVPTCIEQIHVVPPCRKLCEKVYSDCKKLIDTFGIRWPEELE
                     CDRLQYCDEGPGDEEVPLPHKTPIQPGEECHSVGSNSDQYIWVKRSLNCVLKCGYDAGL
                     YSRSAKEFTDIWMAVWASLCFISTTFTVLTFLIDSSRFSYPERPIIFLSMCYNIYSIAY
                     IVRLTVGRERISCDFEEAAEPVLIQEGLKNTGCAIIFLLMYFFGMASSIWWVILTLTWF
                     LAAGLKWGHEAIEMHSSYFHIAAWAIPAVKTIVILIMRLVDADELTGLCYVGNQNLDAL
                     TGFVVAPLFTYLVIGTLFIAAGLVALFKIRSNLQKDGTKTDKLERLMVKIGVFSVLYTV
                     PATCVIACYFYEISNWALFRYSADDSNMAVEMLKIFMSLLVGITSGMWIWSAKTLHTWQ
                     KCSNRLVNSGKVKREKRGNGWVKPGKGNETVV"
     primer_bind     complement(394..436)
                     /label=hFz6_131r_mFz4ov
                     /note="color: black; sequence: 
                     CACCTGGTCCCTCATCACAGTATTGTAATCTGTCACATTCAAG"
     primer_bind     417..449
                     /label=mFz4_161f_hFz6ov
                     /note="color: black; sequence: 
                     CTGTGATGAGGGACCAGGTGATGAAGAGGTTCC"
ORIGIN
        1 ggtaccatgt ctgcacttct gatcctagct cttgttggag ctgcagttgc tgactacaaa
       61 gacgatgacg acaagcttgc ggccgcacac agtctcttca cctgtgaacc aattactgtt
      121 cccagatgta tgaaaatggc ctacaacatg acgtttttcc ctaatctgat gggtcattat
      181 gaccagagta ttgccgcggt ggaaatggag cattttcttc ctctcgcaaa tctggaatgt
      241 tcaccaaaca ttgaaacttt cctctgcaaa gcatttgtac caacctgcat agaacaaatt
      301 catgtggttc caccttgtcg taaactttgt gagaaagtat attctgattg caaaaaatta
      361 attgacactt ttgggatccg atggcctgag gagcttgaat gtgacagatt acaatactgt
      421 gatgagggac caggtgatga agaggttccc ttgccccaca agactcccat ccagcccggg
      481 gaagagtgcc actccgtggg aagcaattct gatcagtaca tctgggtgaa gaggagcctg
      541 aactgtgttc tcaagtgtgg ctacgatgct ggcttgtaca gccgctcagc taaggagttc
      601 acggatattt ggatggctgt gtgggccagc ctctgcttca tctccaccac cttcaccgtg
      661 ctgaccttcc tgattgattc atccaggttt tcttaccctg agcgccccat catatttctc
      721 agtatgtgct ataatattta tagcattgct tatattgttc ggctgactgt aggccgggaa
      781 aggatatcct gtgattttga agaggcggca gagcccgttc tcatccaaga aggacttaag
      841 aacacaggat gtgcaataat tttcttgctg atgtactttt ttggaatggc cagctccatt
      901 tggtgggtta ttctgacact cacttggttt ttggcagccg gactcaagtg gggtcatgaa
      961 gccattgaaa tgcacagttc ttatttccac atcgcagcct gggctattcc cgcagtgaaa
     1021 accattgtca tcttgattat gagactagtg gatgccgatg aactgactgg cttgtgctat
     1081 gttgggaacc aaaacctaga tgccctcact ggctttgtgg tggctcctct ctttacgtat
     1141 ttggtgattg gaacgctgtt cattgcggcg ggtttggtgg ccttattcaa aattcggtcc
     1201 aatcttcaaa aagacgggac aaagacagac aagttggaaa ggctaatggt caagatcggg
     1261 gtcttctcag tactgtacac ggttcctgca acctgtgtga ttgcctgtta tttctatgaa
     1321 atctcaaact gggcactctt tcgatattct gcagatgact caaacatggc agttgaaatg
     1381 ttgaaaattt ttatgtcttt gctcgtgggc atcacttcag gcatgtggat ttggtctgcc
     1441 aaaactcttc acacgtggca aaagtgttct aaccgattgg tgaattctgg gaaggtaaag
     1501 agagagaaga gggggaatgg ttgggtgaag ccaggaaaag gcaacgagac tgtggtatag
     1561 ctcgag
//
