LOCUS       Exported                1688 bp DNA     linear   UNA 03-MAR-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 17, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        75..93
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             127..171
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /note="color: #993366
                     Cleavage site after base 171"
                     /translation="MSALLILALVGAAVA"
     CDS             172..195
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     CDS             1609..1638
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /note="color: #cc99b2"
                     /translation="EQKLISEEDL"
ORIGIN
        1 ccccgttggg ggaggctata tagcagagct ctctggctaa ctagagaacc cactgcttac
       61 tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta gttaagcttg
      121 gtaccaatgt ctgcacttct gatcctagct cttgttggag ctgcagttgc tgactacaaa
      181 gacgatgacg acaagcttgc ggccgcagag gagcggcgct gcgaccccat ccgcatcgcc
      241 atgtgccaga acctcggcta caacgtgacc aagatgccca acttagtggg acacgagctg
      301 cagacagacg ccgagctgca gctgacaact ttcacgccgc tcatccagta cggctgctcc
      361 agccagctgc agttcttcct ttgttcggtt tatgtgccaa tgtgcacaga gaagatcaac
      421 atccccatcg gcccgtgcgg tggcatgtgc ctttcagtca agagacgctg tgaaccagtc
      481 ctgagagaat ttgggtttgc ctggcccgac accctgaact gcagcaagtt cccgccccag
      541 aacgaccaca accacatgtg catggaagga ggttcaggag gttgtggcta cgatgctggc
      601 ttgtacagcc gctcagctaa ggagttcacg gatatttgga tggctgtgtg ggccagcctc
      661 tgcttcatct ccaccacctt caccgtgctg accttcctga ttgattcatc caggttttct
      721 taccctgagc gccccatcat atttctcagt atgtgctata atatttatag cattgcttat
      781 attgttcggc tgactgtagg ccgggaaagg atatcctgtg attttgaaga ggcggcagag
      841 cccgttctca tccaagaagg acttaagaac acaggatgtg caataatttt cttgctgatg
      901 tacttttttg gaatggccag ctccatttgg tgggttattc tgacactcac ttggtttttg
      961 gcagccggac tcaagtgggg tcatgaagcc attgaaatgc acagttctta tttccacatc
     1021 gcagcctggg ctattcccgc agtgaaaacc attgtcatct tgattatgag actagtggat
     1081 gccgatgaac tgactggctt gtgctatgtt gggaaccaaa acctagatgc cctcactggc
     1141 tttgtggtgg ctcctctctt tacgtatttg gtgattggaa cgctgttcat tgcggcgggt
     1201 ttggtggcct tattcaaaat tcggtccaat cttcaaaaag acgggacaaa gacagacaag
     1261 ttggaaaggc taatggtcaa gatcggggtc ttctcagtac tgtacacggt tcctgcaacc
     1321 tgtgtgattg cctgttattt ctatgaaatc tcaaactggg cactctttcg atattctgca
     1381 gatgactcaa acatggcagt tgaaatgttg aaaattttta tgtctttgct cgtgggcatc
     1441 acttcaggca tgtggatttg gtctgccaaa actcttcaca cgtggcaaaa gtgttctaac
     1501 cgattggtga attctgggaa ggtaaagaga gagaagaggg ggaatggttg ggtgaagcca
     1561 ggaaaaggca acgagactgt ggtatagctc gagtctagag ggcccttcga acaaaaactc
     1621 atctcagaag aggatctgaa tatgcatacc ggtcatcatc accatcaccc atgagttaac
     1681 ccatttcc
//
