LOCUS       Exported                1705 bp DNA     linear   UNA 24-JUL-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 17, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..29
                     /label=Primer 2
                     /note="color: black; sequence: 
                     GTCGACcATGTCTGCACTTCTGATCCTAGC; added: 2019-04-25"
     CDS             7..51
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /note="color: #993366
                     Cleavage site after base 51"
                     /translation="MSALLILALVGAAVA"
     primer_bind     7..29
                     /label=Primer 4
                     /note="color: black; sequence: 
                     ATCGATaATGTCTGCACTTCTGATCCTAGC; added: 2019-04-25"
     CDS             52..75
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     primer_bind     79..95
                     /label=Primer 1
                     /note="color: black; sequence: acacaGCGGCCGCAGAGGAGCG; 
                     added: 2019-04-16"
     CDS             88..456
                     /codon_start=1
                     /label=Fzd4
                     /note="color: #993366"
                     /translation="EERRCDPIRIAMCQNLGYNVTKMPNLVGHELQTDAELQLTTFTPL
                     IQYGCSSQLQFFLCSVYVPMCTEKINIPIGPCGGMCLSVKRRCEPVLREFGFAWPDTLN
                     CSKFPPQNDHNHMCMEGPG"
     CDS             457..663
                     /codon_start=1
                     /label=Fzd5
                     /note="color: #33cccc"
                     /translation="SEATTAPPRPFPAKPTLPGPPGAPASGGECPAGGPFVCKCREPFV
                     PILKESHPLYNKVRTGQVPNCAVP"
     CDS             664..1665
                     /codon_start=1
                     /label=Fzd4
                     /note="color: #993366"
                     /translation="CGYDAGLYSRSAKEFTDIWMAVWASLCFISTTFTVLTFLIDSSRF
                     SYPERPIIFLSMCYNIYSIAYIVRLTVGRERISCDFEEAAEPVLIQEGLKNTGCAIIFL
                     LMYFFGMASSIWWVILTLTWFLAAGLKWGHEAIEMHSSYFHIAAWAIPAVKTIVILIMR
                     LVDADELTGLCYVGNQNLDALTGFVVAPLFTYLVIGTLFIAAGLVALFKIRSNLQKDGT
                     KTDKLERLMVKIGVFSVLYTVPATCVIACYFYEISNWALFRYSADDSNMAVEMLKIFMS
                     LLVGITSGMWIWSAKTLHTWQKCSNRLVNSGKVKREKRGNGWVKPGKGNETVV"
     primer_bind     complement(1642..1665)
                     /label=Primer 5
                     /note="color: black; sequence: 
                     GTCGACTACCACAGTCTCGTTGCCTTTTCC; added: 2019-04-25"
     primer_bind     complement(1643..1665)
                     /label=Primer 3
                     /note="color: black; sequence: 
                     CTCGAGcTACCACAGTCTCGTTGCCTTTTC; added: 2019-04-25"
     CDS             1680..1697
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
ORIGIN
        1 ggtaccatgt ctgcacttct gatcctagct cttgttggag ctgcagttgc tgactacaaa
       61 gacgatgacg acaagcttgc ggccgcagag gagcggcgct gcgaccccat ccgcatcgcc
      121 atgtgccaga acctcggcta caacgtgacc aagatgccca acttagtggg acacgagctg
      181 cagacagacg ccgagctgca gctgacaact ttcacgccgc tcatccagta cggctgctcc
      241 agccagctgc agttcttcct ttgttcggtt tatgtgccaa tgtgcacaga gaagatcaac
      301 atccccatcg gcccgtgcgg tggcatgtgc ctttcagtca agagacgctg tgaaccagtc
      361 ctgagagaat ttgggtttgc ctggcccgac accctgaact gcagcaagtt cccgccccag
      421 aacgaccaca accacatgtg catggaagga ccaggtagcg aggccaccac ggcgcccccc
      481 aggcctttcc cagccaagcc cacccttcca ggcccgccag gggcgccggc ctcggggggc
      541 gaatgccccg ctgggggccc gttcgtgtgc aagtgtcgcg agcccttcgt gcccattctg
      601 aaggagtcac acccgctcta caacaaggtg cggacgggcc aggtgcccaa ctgcgcggta
      661 ccctgtggct acgatgctgg cttgtacagc cgctcagcta aggagttcac ggatatttgg
      721 atggctgtgt gggccagcct ctgcttcatc tccaccacct tcaccgtgct gaccttcctg
      781 attgattcat ccaggttttc ttaccctgag cgccccatca tatttctcag tatgtgctat
      841 aatatttata gcattgctta tattgttcgg ctgactgtag gccgggaaag gatatcctgt
      901 gattttgaag aggcggcaga gcccgttctc atccaagaag gacttaagaa cacaggatgt
      961 gcaataattt tcttgctgat gtactttttt ggaatggcca gctccatttg gtgggttatt
     1021 ctgacactca cttggttttt ggcagccgga ctcaagtggg gtcatgaagc cattgaaatg
     1081 cacagttctt atttccacat cgcagcctgg gctattcccg cagtgaaaac cattgtcatc
     1141 ttgattatga gactagtgga tgccgatgaa ctgactggct tgtgctatgt tgggaaccaa
     1201 aacctagatg ccctcactgg ctttgtggtg gctcctctct ttacgtattt ggtgattgga
     1261 acgctgttca ttgcggcggg tttggtggcc ttattcaaaa ttcggtccaa tcttcaaaaa
     1321 gacgggacaa agacagacaa gttggaaagg ctaatggtca agatcggggt cttctcagta
     1381 ctgtacacgg ttcctgcaac ctgtgtgatt gcctgttatt tctatgaaat ctcaaactgg
     1441 gcactctttc gatattctgc agatgactca aacatggcag ttgaaatgtt gaaaattttt
     1501 atgtctttgc tcgtgggcat cacttcaggc atgtggattt ggtctgccaa aactcttcac
     1561 acgtggcaaa agtgttctaa ccgattggtg aattctggga aggtaaagag agagaagagg
     1621 gggaatggtt gggtgaagcc aggaaaaggc aacgagactg tggtatagct cgactcgagc
     1681 atcatcacca tcaccatgag taacc
//
