LOCUS       Exported                1759 bp DNA     linear   UNA 09-NOV-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 17, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        70..88
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             121..165
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /note="color: #993366
                     Cleavage site after base 165"
                     /translation="MSALLILALVGAAVA"
     CDS             166..189
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     CDS             202..561
                     /codon_start=1
                     /label=Fzd4
                     /note="color: #993366"
                     /translation="EERRCDPIRIAMCQNLGYNVTKMPNLVGHELQTDAELQLTTFTPL
                     IQYGCSSQLQFFLCSVYVPMCTEKINIPIGPCGGMCLSVKRRCEPVLREFGFAWPDTLN
                     CSKFPPQNDHNHMCME"
     primer_bind     complement(538..570)
                     /label=Primer 1
                     /note="color: black; sequence: 
                     AGGAACAGTTTCCATGCACATGTGGTTGTGGTC; added: 2021-11-09"
     primer_bind     553..588
                     /label=Primer 2
                     /note="color: black; sequence: 
                     TGCATGGAAACTGTTCCTGTAACTTTTGATCCACAC; added: 2021-11-09"
     CDS             562..720
                     /codon_start=1
                     /label=Fzd6
                     /note="color: #339966"
                     /translation="TVPVTFDPHTEFLGPQKKTEQVQRDIGFWCPRHLKTSGGQGYKFL
                     GIDQCAPP"
     primer_bind     complement(702..720)
                     /label=Fzd4_6linker xho1
                     /note="color: black; sequence: 
                     acacaCTCGAGTGGAGGCGCACACTGGTCA"
     primer_bind     complement(703..729)
                     /label=Primer 3
                     /note="color: black; sequence: GTAGCCACATGGAGGCGCACACTGGTC;
                     added: 2021-11-09"
     primer_bind     complement(703..720)
                     /label=Fzd4_6linker c Ecor1
                     /note="color: black; sequence: 
                     acacaGAATTCcTGGAGGCGCACACTGGTC"
     primer_bind     712..737
                     /label=Primer 4
                     /note="color: black; sequence: GCGCCTCCATGTGGCTACGATGCTGG; 
                     added: 2021-11-09"
     CDS             721..1722
                     /codon_start=1
                     /label=Fzd4
                     /note="color: #993366"
                     /translation="CGYDAGLYSRSAKEFTDIWMAVWASLCFISTTFTVLTFLIDSSRF
                     SYPERPIIFLSMCYNIYSIAYIVRLTVGRERISCDFEEAAEPVLIQEGLKNTGCAIIFL
                     LMYFFGMASSIWWVILTLTWFLAAGLKWGHEAIEMHSSYFHIAAWAIPAVKTIVILIMR
                     LVDADELTGLCYVGNQNLDALTGFVVAPLFTYLVIGTLFIAAGLVALFKIRSNLQKDGT
                     KTDKLERLMVKIGVFSVLYTVPATCVIACYFYEISNWALFRYSADDSNMAVEMLKIFMS
                     LLVGITSGMWIWSAKTLHTWQKCSNRLVNSGKVKREKRGNGWVKPGKGNETVV"
     CDS             1732..1749
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
ORIGIN
        1 cgggggggag catataagca gagctctctg gctaactaga gaacccactg cttactggct
       61 tatcgaaatt aatacgactc actataggga gacccaagct ggctagttaa gcttggtacc
      121 atgtctgcac ttctgatcct agctcttgtt ggagctgcag ttgctgacta caaagacgat
      181 gacgacaagc ttgcggccgc agaggagcgg cgctgcgacc ccatccgcat cgccatgtgc
      241 cagaacctcg gctacaacgt gaccaagatg cccaacttag tgggacacga gctgcagaca
      301 gacgccgagc tgcagctgac aactttcacg ccgctcatcc agtacggctg ctccagccag
      361 ctgcagttct tcctttgttc ggtttatgtg ccaatgtgca cagagaagat caacatcccc
      421 atcggcccgt gcggtggcat gtgcctttca gtcaagagac gctgtgaacc agtcctgaga
      481 gaatttgggt ttgcctggcc cgacaccctg aactgcagca agttcccgcc ccagaacgac
      541 cacaaccaca tgtgcatgga aactgttcct gtaacttttg atccacacac agaatttctt
      601 ggtcctcaga agaaaacaga acaagtccaa agagacattg gattttggtg tccaaggcat
      661 cttaagactt ctgggggaca aggatataag tttctgggaa ttgaccagtg tgcgcctcca
      721 tgtggctacg atgctggctt gtacagccgc tcagctaagg agttcacgga tatttggatg
      781 gctgtgtggg ccagcctctg cttcatctcc accaccttca ccgtgctgac cttcctgatt
      841 gattcatcca ggttttctta ccctgagcgc cccatcatat ttctcagtat gtgctataat
      901 atttatagca ttgcttatat tgttcggctg actgtaggcc gggaaaggat atcctgtgat
      961 tttgaagagg cggcagagcc cgttctcatc caagaaggac ttaagaacac aggatgtgca
     1021 ataattttct tgctgatgta cttttttgga atggccagct ccatttggtg ggttattctg
     1081 accctcactt ggtttttggc agccggactc aagtggggtc atgaagccat tgaaatgcac
     1141 agttcttatt tccacatcgc agcctgggct attcccgcag tgaaaaccat tgtcatcttg
     1201 attatgagac tagtggatgc cgatgaactg actggcttgt gctatgttgg gaaccaaaac
     1261 ctagatgccc tcactggctt tgtggtggct cctctcttta cgtatttggt gattggaacg
     1321 ctgttcattg cggcgggttt ggtggcctta ttcaaaattc ggtccaatct tcaaaaagac
     1381 gggacaaaga cagacaagtt ggaaaggcta atggtcaaga tcggggtctt ctcagtactg
     1441 tacacggttc ctgcaacctg tgtgattgcc tgttatttct atgaaatctc aaactgggca
     1501 ctctttcgat attctgcaga tgactcaaac atggcagttg aaatgttgaa aatttttatg
     1561 tctttgctcg tgggcatcac ttcaggcatg tggatttggt ctgccaaaac tcttcacacg
     1621 tggcaaaagt gttctaaccg attggtgaat tctgggaagg taaagagaga gaagaggggg
     1681 aatggttggg tgaagccagg aaaaggcaac gagactgtgg tatagctcga gcatcatcac
     1741 catcaccatg agaacttgc
//
