LOCUS       Exported                1747 bp DNA     linear   UNA 21-SEP-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 17, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        77..95
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             130..174
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /note="color: #993366
                     Cleavage site after base 174"
                     /translation="MSALLILALVGAAVA"
     CDS             175..198
                     /codon_start=1
                     /product="FLAG epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     CDS             211..708
                     /codon_start=1
                     /label=Fzd3
                     /note="color: #993366"
                     /translation="HSLFSCEPITLRMCQDLPYNTTFMPNLLNHYDQQTAALAMEPFHP
                     MVNLDCSRDFRPFLCALYAPICMEYGRVTLPCRRLCQRAYSECSKLMEMFGVPWPEDME
                     CSRFPDCDEPYPRLVDLNLAGEPTEGAPVAVQRDYGFWCPRELKIDPDLGYSFLHVRDC
                     SPP"
     CDS             709..1710
                     /codon_start=1
                     /label=Fzd4
                     /note="color: #99cc00"
                     /translation="CGYDAGLYSRSAKEFTDIWMAVWASLCFISTTFTVLTFLIDSSRF
                     SYPERPIIFLSMCYNIYSIAYIVRLTVGRERISCDFEEAAEPVLIQEGLKNTGCAIIFL
                     LMYFFGMASSIWWVILTLTWFLAAGLKWGHEAIEMHSSYFHIAAWAIPAVKTIVILIMR
                     LVDADELTGLCYVGNQNLDALTGFVVAPLFTYLVIGTLFIAAGLVALFKIRSNLQKDGT
                     KTDKLERLMVKIGVFSVLYTVPATCVIACYFYEISNWALFRYSADDSNMAVEMLKIFMS
                     LLVGITSGMWIWSAKTLHTWQKCSNRLVNSGKVKREKRGNGWVKPGKGNETVV"
ORIGIN
        1 cccaatgggg ggaaagctat atagcagaag ctctctggct aactagagaa cccactgctt
       61 actggcttat cgaaattaat acgactcact atagggagac ccaagctggc tagttaagct
      121 tggtaccgaa tgtctgcact tctgatccta gctcttgttg gagctgcagt tgctgactac
      181 aaagacgatg acgacaagct tgcggccgca cacagtttgt tttcttgtga acctattacc
      241 ttgaggatgt gccaagattt gccttataat actaccttca tgcctaatct tctgaatcat
      301 tatgaccaac agacagcagc tttggcaatg gagccattcc accctatggt gaatctggat
      361 tgttctcggg atttccggcc ttttctttgt gcactctacg ctcctatttg tatggaatat
      421 ggacgtgtca cacttccctg tcgtaggctg tgtcagcggg cttacagtga gtgttcgaag
      481 ctcatggaga tgtttggtgt tccttggcct gaagatatgg aatgcagtag gttcccagat
      541 tgtgatgagc catatcctcg acttgtggat ctgaatttag ctggagaacc aactgaagga
      601 gccccagtgg cagtgcagag agactatggt ttttggtgtc cccgagagtt aaaaattgat
      661 cctgatctgg gttattcttt tctgcatgtg cgtgattgtt cacctccttg tggctacgat
      721 gctggcttgt acagccgctc agctaaggag ttcacggata tttggatggc tgtgtgggcc
      781 agcctctgct tcatctccac caccttcacc gtgctgacct tcctgattga ttcatccagg
      841 ttttcttacc ctgagcgccc catcatattt ctcagtatgt gctataatat ttatagcatt
      901 gcttatattg ttcggctgac tgtaggccgg gaaaggatat cctgtgattt tgaagaggcg
      961 gcagagcccg ttctcatcca agaaggactt aagaacacag gatgtgcaat aattttcttg
     1021 ctgatgtact tttttggaat ggccagctcc atttggtggg ttattctgac actcacttgg
     1081 tttttggcag ccggactcaa gtggggtcat gaagccattg aaatgcacag ttcttatttc
     1141 cacatcgcag cctgggctat tcccgcagtg aaaaccattg tcatcttgat tatgagacta
     1201 gtggatgccg atgaactgac tggcttgtgc tatgttggga accaaaacct agatgccctc
     1261 actggctttg tggtggctcc tctctttacg tatttggtga ttggaacgct gttcattgcg
     1321 gcgggtttgg tggccttatt caaaattcgg tccaatcttc aaaaagacgg gacaaagaca
     1381 gacaagttgg aaaggctaat ggtcaagatc ggggtcttct cagtactgta cacggttcct
     1441 gcaacctgtg tgattgcctg ttatttctat gaaatctcaa actgggcact ctttcgatat
     1501 tctgcagatg actcaaacat ggcagttgaa atgttgaaaa tttttatgtc tttgctcgtg
     1561 ggcatcactt caggcatgtg gatttggtct gccaaaactc ttcacacgtg gcaaaagtgt
     1621 tctaaccgat tggtgaattc tgggaaggta aagagagaga agagggggaa tggttgggtg
     1681 aagccaggaa aaggcaacga gactgtggta tagctcgagc atcatcaccc atcaccactg
     1741 ggggtta
//
