LOCUS       Exported                1841 bp DNA     linear   UNA 09-NOV-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 17, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        76..94
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             127..171
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /note="color: #993366
                     Cleavage site after base 171"
                     /translation="MSALLILALVGAAVA"
     CDS             172..195
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     CDS             208..792
                     /codon_start=1
                     /label=FZD5_CRDlinker
                     /note="color: #993366"
                     /translation="ASKAPVCQEITVPMCRGIGYNLTHMPNQFNHDTQDEAGLEVHQFW
                     PLVEIQCSPDLRFFLCSMYTPICLPDYHKPLPPCRSVCERAKAGCSPLMRQYGFAWPER
                     MSCDRLPVLGRDAEVLCMDYNRSEATTAPPRPFPAKPTLPGPPGAPASGGECPAGGPFV
                     CKCREPFVPILKESHPLYNKVRTGQVPNCAVP"
     primer_bind     complement(776..801)
                     /label=Primer 1
                     /note="color: black; sequence: GTAGCCACAGGGTACCGCGCAGTTGG; 
                     added: 2021-11-09"
     primer_bind     784..810
                     /label=Primer 2
                     /note="color: black; sequence: GCGGTACCCTGTGGCTACGATGCTGGC;
                     added: 2021-11-09"
     CDS             793..1794
                     /codon_start=1
                     /label=Fzd4
                     /note="color: #99cc00"
                     /translation="CGYDAGLYSRSAKEFTDIWMAVWASLCFISTTFTVLTFLIDSSRF
                     SYPERPIIFLSMCYNIYSIAYIVRLTVGRERISCDFEEAAEPVLIQEGLKNTGCAIIFL
                     LMYFFGMASSIWWVILTLTWFLAAGLKWGHEAIEMHSSYFHIAAWAIPAVKTIVILIMR
                     LVDADELTGLCYVGNQNLDALTGFVVAPLFTYLVIGTLFIAAGLVALFKIRSNLQKDGT
                     KTDKLERLMVKIGVFSVLYTVPATCVIACYFYEISNWALFRYSADDSNMAVEMLKIFMS
                     LLVGITSGMWIWSAKTLHTWQKCSNRLVNSGKVKREKRGNGWVKPGKGNETVV"
     CDS             1804..1821
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
ORIGIN
        1 cccatttggg gagccaatat aaggcagagc tctctggcta actagagaac ccactgctta
       61 ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct agttaagctt
      121 ggtaccatgt ctgcacttct gatcctagct cttgttggag ctgcagttgc tgactacaaa
      181 gacgatgacg acaagcttgc ggccgcagcg tccaaggccc cggtgtgcca ggaaatcacg
      241 gtgcccatgt gccgcggcat cggctacaac ctgacgcaca tgcccaacca gttcaaccac
      301 gacacgcagg acgaggcggg cctggaggtg caccagttct ggccgctggt ggagatccaa
      361 tgctcgccgg acctgcgctt cttcctatgc tctatgtaca cgcccatctg tctgcccgac
      421 taccacaagc cgctgccgcc ctgccgctcg gtgtgcgagc gcgccaaggc cggctgctcg
      481 ccgctgatgc gccagtacgg cttcgcctgg cccgagcgca tgagctgcga ccgcctcccg
      541 gtgctgggcc gcgacgccga ggtcctctgc atggattaca accgcagcga ggccaccacg
      601 gcgcccccca ggcctttccc agccaagccc acccttccag gcccgccagg ggcgccggcc
      661 tcggggggcg aatgccccgc tgggggcccg ttcgtgtgca agtgtcgcga gcccttcgtg
      721 cccattctga aggagtcaca cccgctctac aacaaggtgc ggacgggcca ggtgcccaac
      781 tgcgcggtac cctgtggcta cgatgctggc ttgtacagcc gctcagctaa ggagttcacg
      841 gatatttgga tggctgtgtg ggccagcctc tgcttcatct ccaccacctt caccgtgctg
      901 accttcctga ttgattcatc caggttttct taccctgagc gccccatcat atttctcagt
      961 atgtgctata atatttatag cattgcttat attgttcggc tgactgtagg ccgggaaagg
     1021 atatcctgtg attttgaaga ggcggcagag cccgttctca tccaagaagg acttaagaac
     1081 acaggatgtg caataatttt cttgctgatg tacttttttg gaatggccag ctccatttgg
     1141 tgggttattc tgacactcac ttggtttttg gcagccggac tcaagtgggg tcatgaagcc
     1201 attgaaatgc acagttctta tttccacatc gcagcctggg ctattcccgc agtgaaaacc
     1261 attgtcatct tgattatgag actagtggat gccgatgaac tgactggctt gtgctatgtt
     1321 gggaaccaaa acctagatgc cctcactggc tttgtggtgg ctcctctctt tacgtatttg
     1381 gtgattggaa cgctgttcat tgcggcgggt ttggtggcct tattcaaaat tcggtccaat
     1441 cttcaaaaag acgggacaaa gacagacaag ttggaaaggc taatggtcaa gatcggggtc
     1501 ttctcagtac tgtacacggt tcctgcaacc tgtgtgattg cctgttattt ctatgaaatc
     1561 tcaaactggg cactctttcg atattctgca gatgactcaa acatggcagt tgaaatgttg
     1621 aaaattttta tgtctttgct cgtgggcatc acttcaggca tgtggatttg gtctgccaaa
     1681 actcttcaca cgtggcaaaa gtgttctaac cgattggtga attctgggaa ggtaaagaga
     1741 gagaagaggg ggaatggttg ggtgaagcca ggaaaaggca acgagactgt ggtatagctc
     1801 gagcatcatc accatcacca tgagttaacc ccctgatccc c
//
