LOCUS       Exported                1751 bp DNA     linear   UNA 20-APR-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 17, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        74..92
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             126..170
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /note="color: #993366
                     Cleavage site after base 170"
                     /translation="MSALLILALVGAAVA"
     CDS             171..194
                     /codon_start=1
                     /product="FLAG epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     CDS             1716..1733
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
ORIGIN
        1 cccgaatggg gaggctatat agcagagctc tctggctaac tagagaaccc actgcttact
       61 ggcttatcga aattaatacg actcactata gggagaccca agctggctag ttaagcttgg
      121 taccaatgtc tgcacttctg atcctagctc ttgttggagc tgcagttgct gactacaaag
      181 acgatgacga caagcttgcg gccgcacaca gtctcttcac ctgtgaacca attactgttc
      241 ccagatgtat gaaaatggcc tacaacatga cgtttttccc taatctgatg ggtcattatg
      301 accagagtat tgccgcggtg gaaatggagc attttcttcc tctcgcaaat ctggaatgtt
      361 caccaaacat tgaaactttc ctctgcaaag catttgtacc aacctgcata gaacaaattc
      421 atgtggttcc accttgtcgt aaactttgtg agaaagtata ttctgattgc aaaaaattaa
      481 ttgacacttt tgggatccga tggcctgagg agcttgaatg tgacagatta caatactgtg
      541 atgagactgt tcctgtaact tttgatccac acacagaatt tcttggtcct cagaagaaaa
      601 cagaacaagt ccaaagagac attggatttt ggtgtccaag gcatcttaag acttctgggg
      661 gacaaggata taagtttctg ggaattgacc agtgtgcgcc tccatgtggc tacgatgctg
      721 gcttgtacag ccgctcagct aaggagttca cggatatttg gatggctgtg tgggccagcc
      781 tctgcttcat ctccaccacc ttcaccgtgc tgaccttcct gattgattca tccaggtttt
      841 cttaccctga gcgccccatc atatttctca gtatgtgcta taatatttat agcattgctt
      901 atattgttcg gctgactgta ggccgggaaa ggatatcctg tgattttgaa gaggcggcag
      961 agcccgttct catccaagaa ggacttaaga acacaggatg tgcaataatt ttcttgctga
     1021 tgtacttttt tggaatggcc agctccattt ggtgggttat tctgacactc acttggtttt
     1081 tggcagccgg actcaagtgg ggtcatgaag ccattgaaat gcacagttct tatttccaca
     1141 tcgcagcctg ggctattccc gcagtgaaaa ccattgtcat cttgattatg agactagtgg
     1201 atgccgatga actgactggc ttgtgctatg ttgggaacca aaacctagat gccctcactg
     1261 gctttgtggt ggctcctctc tttacgtatt tggtgattgg aacgctgttc attgcggcgg
     1321 gtttggtggc cttattcaaa attcggtcca atcttcaaaa agacgggaca aagacagaca
     1381 agttggaaag gctaatggtc aagatcgggg tcttctcagt actgtacacg gttcctgcaa
     1441 cctgtgtgat tgcctgttat ttctatgaaa tctcaaactg ggcactcttt cgatattctg
     1501 cagatgactc aaacatggca gttgaaatgt tgaaaatttt tatgtctttg ctcgtgggca
     1561 tcacttcagg catgtggatt tggtctgcca aaactcttca cacgtggcaa aagtgttcta
     1621 accgattggt gaattctggg aaggtaaaga gagagaagag ggggaatggt tgggtgaagc
     1681 caggaaaagg caacgagact gtggtatagc tcgagcatca tcaccatcac catgaagttt
     1741 aaacccgttc a
//
