LOCUS       Exported                1977 bp DNA     linear   UNA 09-NOV-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 17, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        90..108
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             142..186
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /note="color: #993366
                     Cleavage site after base 186"
                     /translation="MSALLILALVGAAVA"
     CDS             187..210
                     /codon_start=1
                     /product="FLAG epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     CDS             223..711
                     /codon_start=1
                     /label=FZD4CRDlinker
                     /note="color: #339966"
                     /translation="EERRCDPIRIAMCQNLGYNVTKMPNLVGHELQTDAELQLTTFTPL
                     IQYGCSSQLQFFLCSVYVPMCTEKINIPIGPCGGMCLSVKRRCEPVLREFGFAWPDTLN
                     CSKFPPQNDHNHMCMEGPGDEEVPLPHKTPIQPGEECHSVGSNSDQYIWVKRSLNCVLK
                     "
     primer_bind     complement(688..720)
                     /label=Primer 1
                     /note="color: black; sequence: 
                     CTGGTAGCACTTGAGAACACAGTTCAGGCTCCT; added: 2021-11-09"
     primer_bind     703..731
                     /label=Primer 2
                     /note="color: black; sequence: 
                     GTTCTCAAGTGCTACCAGCCGTCCTTCAG; added: 2021-11-09"
     CDS             712..1803
                     /codon_start=1
                     /label=Fzd5
                     /note="color: #993366"
                     /translation="CYQPSFSADERTFATFWIGLWSVLCFISTSTTVATFLIDMERFRY
                     PERPIIFLSACYLCVSLGFLVRLVVGHASVACSREHNHIHYETTGPALCTIVFLLVYFF
                     GMASSIWWVILSLTWFLAAGMKWGNEAIAGYAQYFHLAAWLIPSVKSITALALSSVDGD
                     PVAGICYVGNQNLNSLRGFVLGPLVLYLLVGTLFLLAGFVSLFRIRSVIKQGGTKTDKL
                     EKLMIRIGIFTLLYTVPASIVVACYLYEQHYRESWEAALTCACPGHDTGQPRAKPEYWV
                     LMLKYFMCLVVGITSGVWIWSGKTVESWRRFTSRCCCRPRRGHKSGGAMAAGDYPEASA
                     ALTGRTGPPGPAATYHKQVSLSHV"
ORIGIN
        1 gcacagtcat actaatgtta ttttccattg ggctttagca gagctctctg gctaactaga
       61 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
      121 ggctagttaa gcttggtacc aatgtctgca cttctgatcc tagctcttgt tggagctgca
      181 gttgctgact acaaagacga tgacgacaag cttgcggccg cagaggagcg gcgctgcgac
      241 cccatccgca tcgccatgtg ccagaacctc ggctacaacg tgaccaagat gcccaactta
      301 gtgggacacg agctgcagac agacgccgag ctgcagctga caactttcac gccgctcatc
      361 cagtacggct gctccagcca gctgcagttc ttcctttgtt cggtttatgt gccaatgtgc
      421 acagagaaga tcaacatccc catcggcccg tgcggtggca tgtgcctttc agtcaagaga
      481 cgctgtgaac cagtcctgag agaatttggg tttgcctggc ccgacaccct gaactgcagc
      541 aagttcccgc cccagaacga ccacaaccac atgtgcatgg aaggaccagg tgatgaagag
      601 gttcccttgc cccacaagac tcccatccag cccggggaag agtgccactc cgtgggaagc
      661 aattctgatc agtacatctg ggtgaagagg agcctgaact gtgttctcaa gtgctaccag
      721 ccgtccttca gtgccgacga gcgcacgttc gccaccttct ggataggcct gtggtcggtg
      781 ctgtgcttca tctccacgtc caccacagtg gccaccttcc tcatcgacat ggaacgcttc
      841 cgctatcctg agcgccccat catcttcctg tcagcctgct acctgtgcgt gtcgctgggc
      901 ttcctggtgc gtctggtcgt gggccatgcc agcgtggcct gcagccgcga gcacaaccac
      961 atccactacg agaccacggg ccctgcactg tgcaccatcg tcttcctcct ggtctacttc
     1021 ttcggcatgg ccagctccat ctggtgggtc atcctgtcgc tcacctggtt cctggccgcc
     1081 ggcatgaagt ggggcaacga ggccatcgcg ggctacgcgc agtacttcca cctggctgcg
     1141 tggctcatcc ccagcgtcaa gtccatcacg gcactggcgc tgagctccgt ggacggggac
     1201 ccagtggccg gcatctgcta cgtgggcaac cagaacctga actcgctgcg cggcttcgtg
     1261 ctgggcccgc tggtgctcta cctgctggtg ggcacgctct tcctgctggc gggcttcgtg
     1321 tcgctcttcc gcatccgcag cgtcatcaag cagggcggca ccaagacgga caagctggag
     1381 aagctcatga tccgcatcgg catcttcacg ctgctctata cggtccccgc cagcattgtg
     1441 gtggcctgct acctgtacga gcagcactac cgcgagagct gggaggcggc gctcacctgc
     1501 gcctgcccgg gccacgacac cggccagccg cgcgccaagc ccgagtactg ggtgctcatg
     1561 ctcaagtact tcatgtgcct ggtggtgggc atcacgtcgg gcgtctggat ctggtcgggc
     1621 aagacggtgg agtcgtggcg gcgtttcacc agccgctgct gctgccgccc gcggcgcggc
     1681 cacaagagcg ggggcgccat ggccgcaggg gactaccccg aggcgagcgc cgcgctcaca
     1741 ggcaggaccg ggccgccggg ccccgccgcc acctaccaca agcaggtgtc cctgtcgcac
     1801 gtgtaagaat tcggtggtgg tggttcaatg gtgagcaagg gcgaggagct gttcaccggg
     1861 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcggtccg
     1921 gcgagggcga gggcgtgccc ctccgcaccc ctccaccccc caccaacacc gggcccg
//
