LOCUS       Exported                2377 bp DNA     linear   UNA 13-APR-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 17, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2377
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        72..90
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             123..167
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /note="color: #993366
                     Cleavage site after base 167"
                     /translation="MSALLILALVGAAVA"
     CDS             168..191
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     CDS             204..692
                     /codon_start=1
                     /label=Fzd4
                     /note="color: #993366"
                     /translation="EERRCDPIRIAMCQNLGYNVTKMPNLVGHELQTDAELQLTTFTPL
                     IQYGCSSQLQFFLCSVYVPMCTEKINIPIGPCGGMCLSVKRRCEPVLREFGFAWPDTLN
                     CSKFPPQNDHNHMCMEGPGDEEVPLPHKTPIQPGEECHSVGSNSDQYIWVKRSLNCVLK
                     "
     misc_feature    555..563
                     /label=Feature 5
                     /note="color: #a6acb3"
     CDS             693..2258
                     /codon_start=1
                     /label=Fzd6
                     /note="color: #339966"
                     /translation="CPNMYFKSDELEFAKSFIGTVSIFCLCATLFTFLTFLIDVRRFRY
                     PERPIIYYSVCYSIVSLMYFIGFLLGDSTACNKADEKLELGDTVVLGSQNKACTVLFML
                     LYFFTMAGTVWWVILTITWFLAAGRKWSCEAIEQKAVWFHAVAWGTPGFLTVMLLALNK
                     VEGDNISGVCFVGLYDLDASRYFVLLPLCLCVFVGLSLLLAGIISLNHVRQVIQHDGRN
                     QEKLKKFMIRIGVFSGLYLVPLVTLLGCYVYEQVNRITWEITWVSDHCRQYHIPCPYQA
                     KAKARPELALFMIKYLMTLIVGISAVFWVGSKKTCTEWAGFFKRNRKRDPISESRRVLQ
                     ESCEFFLKHNSKVKHKKKHYKPSSHKLKVISKSMGTSTGATANHGTSAVAITSHDYLGQ
                     ETLTEIQTSPETSMREVKADGASTPRLREQDCGEPASPAASISRLSGEQVDGKGQAGSV
                     SESARSEGRISPKSDITDTGLAQSNNLQVPSSSEPSSLKGSTSLLVHPVSGVRKEQGGG
                     CHSDT"
     CDS             2347..2364
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
ORIGIN
        1 ccgatgtggg aagcatatag cagagctctc tggctaacta gagaacccac tgcttactgg
       61 cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagtt aagcttggta
      121 ccatgtctgc acttctgatc ctagctcttg ttggagctgc agttgctgac tacaaagacg
      181 atgacgacaa gcttgcggcc gcagaggagc ggcgctgcga ccccatccgc atcgccatgt
      241 gccagaacct cggctacaac gtgaccaaga tgcccaactt agtgggacac gagctgcaga
      301 cagacgccga gctgcagctg acaactttca cgccgctcat ccagtacggc tgctccagcc
      361 agctgcagtt cttcctttgt tcggtttatg tgccaatgtg cacagagaag atcaacatcc
      421 ccatcggccc gtgcggtggc atgtgccttt cagtcaagag acgctgtgaa ccagtcctga
      481 gagaatttgg gtttgcctgg cccgacaccc tgaactgcag caagttcccg ccccagaacg
      541 accacaacca catgtgcatg gaaggaccag gtgatgaaga ggttcccttg ccccacaaga
      601 ctcccatcca gcccggggaa gagtgccact ccgtgggaag caattctgat cagtacatct
      661 gggtgaagag gagcctgaac tgtgttctca agtgccccaa catgtatttt aaaagtgatg
      721 agctagagtt tgcaaaaagt tttattggaa cagtttcaat attttgtctt tgtgcaactc
      781 tgttcacatt ccttactttt ttaattgatg ttagaagatt cagataccca gagagaccaa
      841 ttatatatta ctctgtctgt tacagcattg tatctcttat gtacttcatt ggatttttgc
      901 tgggcgatag cacagcctgc aataaggcag atgagaagct agaacttggt gacactgttg
      961 tcctaggctc tcaaaataag gcttgcaccg ttttgttcat gcttttgtat tttttcacaa
     1021 tggctggcac tgtgtggtgg gtgattctta ccattacttg gttcttagct gcaggaagaa
     1081 aatggagttg tgaagccatc gagcaaaaag cagtgtggtt tcatgctgtt gcatggggaa
     1141 caccaggttt cctgactgtt atgcttcttg ctctgaacaa agttgaagga gacaacatta
     1201 gtggagtttg ctttgttggc ctttatgacc tggatgcttc tcgctacttt gtactcttgc
     1261 cactgtgcct ttgtgtgttt gttgggctct ctcttctttt agctggcatt atttccttaa
     1321 atcatgttcg acaagtcata caacatgatg gccggaacca agaaaaacta aagaaattta
     1381 tgattcgaat tggagtcttc agcggcttgt atcttgtgcc attagtgaca cttctcggat
     1441 gttacgtcta tgagcaagtg aacaggatta cctgggagat aacttgggtc tctgatcatt
     1501 gtcgtcagta ccatatccca tgtccttatc aggcaaaagc aaaagctcga ccagaattgg
     1561 ctttatttat gataaaatac ctgatgacat taattgttgg catctctgct gtcttctggg
     1621 ttggaagcaa aaagacatgc acagaatggg ctgggttttt taaacgaaat cgcaagagag
     1681 atccaatcag tgaaagtcga agagtactac aggaatcatg tgagtttttc ttaaagcaca
     1741 attctaaagt taaacacaaa aagaagcact ataaaccaag ttcacacaag ctgaaggtca
     1801 tttccaaatc catgggaacc agcacaggag ctacagcaaa tcatggcact tctgcagtag
     1861 caattactag ccatgattac ctaggacaag aaactttgac agaaatccaa acctcaccag
     1921 aaacatcaat gagagaggtg aaagcggacg gagctagcac ccccaggtta agagaacagg
     1981 actgtggtga acctgcctcg ccagcagcat ccatctccag actttctggg gaacaggtcg
     2041 acgggaaggg ccaggcaggc agtgtatctg aaagtgcgcg gagtgaagga aggattagtc
     2101 caaagagtga tattactgac actggcctgg cacagagcaa caatttgcag gtccccagtt
     2161 cttcagaacc aagcagcctc aaaggttcca catctctgct tgttcaccca gtttcaggag
     2221 tgagaaaaga gcagggaggt ggttgtcatt cagatacttg agaattctgg gaaggtaaag
     2281 agagagaaga gggggaatgg ttgggtgaag ccaggaaaag gcaacgagac tgtggtatag
     2341 ctcgagcatc atcaccatca ccatgagtag ccatggc
//
