LOCUS       Exported                5847 bp ds-DNA     circular SYN 21-NOV-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pDisplay-V5-NXPH1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Howarth M, Takao K, Hayashi Y, Ting AY
  TITLE     Targeting quantum dots to surface proteins in living cells with 
            biotin ligase.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 May 24. 102(21):7583-8.
  PUBMED    15897449
REFERENCE   2  (bases 1 to 5847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 22, 2022 from SnapGene 5.1.0
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 May 24. 102(21):7583-8."
FEATURES             Location/Qualifiers
     source          1..5847
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #a6acb3"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate earlypromoter"
                     /note="color: #ffffff"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff"
     misc_feature    719..784
                     /label=Signal peptide
                     /note="color: #00ff40"
     misc_feature    785..826
                     /label=V5-tag
                     /note="color: #993366"
     misc_feature    827..1585
                     /label=mNXPH1
                     /note="color: #99ccff"
     primer_bind     complement(1597..1614)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse 
                     primer.Also called BGH reverse"
                     /note="color: #a020f0; direction: LEFT"
     polyA_signal    1603..1827
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(1959..2547)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(2039..2058)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: #a020f0; direction: LEFT"
     polyA_signal    2876..2923
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinasepolyadenylation
                     signal (Cole and Stacy, 1985)"
                     /note="color: #a6acb3"
     primer_bind     2948..2967
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
                     /note="color: #a020f0; direction: RIGHT"
     CDS             complement(3155..3943)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #993366"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEHQGL
                     APAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALA
                     TRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3266..3285)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     3870..3889
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        complement(3978..4338)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      3992..4130
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     complement(4049..4068)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     4295..4315
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
                     /note="color: #a020f0; direction: RIGHT"
     CDS             complement(4398..5258)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5021..5040
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        complement(5259..5363)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      5390..5845
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; 
                     arrowindicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     complement(5477..5496)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     5687..5708
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcat
      721 ggagacagac acactcctgc tatgggtact gctgctctgg gttccaggtt ccactggtga
      781 ctatggcaaa cctattccga atcctcttct tggtctcgat tctacaagat ccgccaacct
      841 aacaaatggt gggaaatcgg aactactgaa gtcagggagc agcaaatcca cgctaaagca
      901 catatggaca gaaagcagca aagacttgtc tatcagtcgg ctcctctcac agactttccg
      961 tggtaaagaa aatgacacag atttggacct gcgctatgac accccagaac cttattctga
     1021 gcaagacctc tgggactggc tgaggaactc cacagatctt caggagcctc ggcccagggc
     1081 caaaagacgg cccattgtta aaactggcaa gtttaagaaa atgtttggat ggggtgattt
     1141 tcattccaac atcaaaacag tgaagctaaa cctgttgata actgggaaaa ttgtagatca
     1201 cggcaatgga acctttagtg tatatttcag gcataactcc actggtcaag ggaatgtatc
     1261 tgtcagcttg gtgcccccaa caaaaatcgt ggaattcgat ttggcacaac aaaccgtgat
     1321 tgatgctaaa gattccaagt ccttcaactg tcgcattgag tatgagaagg ttgacaaggc
     1381 caccaagaac acactctgca actacgaccc ttcaaaaacc tgttaccagg agcagaccca
     1441 aagtcacgtg tcctggctct gctctaagcc cttcaaggtg atctgtattt acatttcctt
     1501 ttatagtaca gactataaac tagtacagaa ggtatgccct gactacaact atcacagtga
     1561 cacaccttac tttccctccg gatgagtcga gatcagcctc gactgtgcct tctagttgcc
     1621 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     1681 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     1741 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     1801 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagt ggcggtaata
     1861 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1921 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1981 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2041 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2101 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2161 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2221 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2281 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2341 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     2401 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2461 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2521 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2581 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2641 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2701 taacctgagg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca
     2761 cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg gccccaatgg
     2821 ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg tttatgaaca
     2881 aacgacccaa caccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct
     2941 tccggtattg tctccttccg tgtttcagtt agcctccccc tagggtgggc gaagaactcc
     3001 agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag
     3061 cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc
     3121 gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat
     3181 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag
     3241 cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc
     3301 ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca
     3361 tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc
     3421 tcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tgatcatcct
     3481 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
     3541 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
     3601 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
     3661 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
     3721 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac
     3781 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
     3841 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
     3901 aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc
     3961 ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc
     4021 tggaatagct cagaggccga ggaggcggcc tcggcctctg cataaataaa aaaaattagt
     4081 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
     4141 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     4201 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata
     4261 cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac attccacagc
     4321 tggttctttc cgcctcagga ctcttccttt ttcaataaat caatctaaag tatatatgag
     4381 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4441 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4501 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4561 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4621 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4681 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4741 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4801 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4861 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4921 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4981 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5041 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5101 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5161 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5221 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5281 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5341 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt
     5401 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5461 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5521 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5581 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5641 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5701 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5761 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     5821 aacaaaatat taacgcttac aatttac
//
