LOCUS       Exported                9445 bp DNA     circular SYN 22-MAR-2022
DEFINITION  .
ACCESSION   VB170530-1031pbc
VERSION     .
KEYWORDS    VB170530-1031pbc.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 22, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9445
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV  promoter
                     /note="/application_notes=Strong promoter; drives 
                     transcriptionof viral RNA in packaging cells."
                     /note="/description=Rous sarcoma virus enhancer/promoter"
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
                     /note="color: #5566f5; direction: RIGHT"
     LTR             230..410
                     /label=Delta-5' LTR
                     /note="/application_notes=Allows transcription of viral RNA
                     andits packaging into virus."
                     /note="/description=Truncated HIV-1 5' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
                     /note="color: #ef6cdf; direction: RIGHT"
     misc_feature    521..565
                     /label=Psi
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus."
                     /note="/description=HIV-1 packaging signal"
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
                     /note="color: #c54b7c; direction: RIGHT"
     misc_feature    1075..1308
                     /label=RRE
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Rev protein binding site that 
                     allowsRev-dependent nuclear export of viral RNA during 
                     viral packaging."
                     /note="/description=HIV-1 Rev response element"
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
                     /note="color: #d05c0a; direction: RIGHT"
     misc_feature    1803..1920
                     /label=cPPT
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1cDNA through a central DNA flap."
                     /note="/description=Central polypurine tract"
                     /note="/full_name=Central polypurine tract"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
                     /note="color: #fd3434; direction: RIGHT"
     promoter        1959..3137
                     /label=EF1A
                     /note="/application_notes=Strong promoter."
                     /note="/description=Human eukaryotic translation 
                     elongationfactor 1 alpha1 promoter"
                     /note="/full_name=Human eukaryotic translation elongation 
                     factor1 alpha1 promoter"
                     /note="/official_designation=EF1A"
                     /note="/uuid=69c052aac71a4f49bab5b7ae1f46ace8"
                     /note="color: #46c6ef; direction: RIGHT"
     misc_feature    3162..3167
                     /label=Kozak
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
                     /note="color: #eda481; direction: RIGHT"
     misc_feature    3168..3914
                     /label=Tet3G
                     /label=nonstandard type:  ORF
                     /note="/application_notes=It binds to TRE3G promoter to 
                     activategene transcription only in the presence of 
                     tetracycline or its analogs (e.g. doxycycline). It has 
                     higher sensitivity to the inducing drug compared to its 
                     predecessor, rtTA."
                     /note="/description=Improved reverse tetracycline 
                     responsivetranscriptional activator (3rd generation)"
                     /note="/full_name=Improved reverse tetracycline 
                     responsivetranscriptional activator (3rd generation)"
                     /note="/official_designation=Tet3G"
                     /note="/uuid=25484972a4bf4858a339bd694b26ad08"
                     /note="color: #8ccc27; direction: RIGHT"
     misc_feature    3953..4550
                     /label=WPRE
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Enhances viral RNA stability 
                     inpackaging cells, leading to higher titer of packaged 
                     virus."
                     /note="/description=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
                     /note="color: #da3bb5; direction: RIGHT"
     promoter        4569..5079
                     /label=mPGK promoter
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Mouse phosphoglycerate kinase 1 
                     promoter"
                     /note="/full_name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/official_designation=mPGK promoter"
                     /note="/uuid=bce93795b5fd4173b8b9abc832edad80"
                     /note="color: #e4b930; direction: RIGHT"
     misc_feature    5089..5883
                     /label=Neo
                     /label=nonstandard type:  Marker
                     /note="/application_notes=Allows cells to be resistant 
                     togeneticin (G418)."
                     /note="/description=Neomycin resistance gene"
                     /note="/full_name=Neomycin resistance gene"
                     /note="/official_designation=Neo"
                     /note="/uuid=c76d56a17a3845f5afec8e8d5a6916c2"
                     /note="color: #7a00b5; direction: RIGHT"
     LTR             5965..6199
                     /label=Delta-U3/3' LTR
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus; self-inactivates the 5' LTR by a copying 
                     mechanism during viral genome integration; contains 
                     polyadenylation signal for transcription termination."
                     /note="/description=Truncated HIV-1 3' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
                     /note="color: #05696d; direction: RIGHT"
     polyA_signal    6272..6406
                     /label=SV40 early pA
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Simian virus 40 early polyadenylation 
                     signal"
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
                     /note="color: #f54c6b; direction: RIGHT"
     misc_feature    7360..8220
                     /label=Ampicillin
                     /label=nonstandard type:  ORF
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampicillin."
                     /note="/description=Ampicillin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
                     /note="color: #944603; direction: RIGHT"
     rep_origin      8391..8979
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
                     /note="color: #ce0084"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg tctagactgg
     3181 acaagagcaa agtcataaac tctgctctgg aattactcaa tggagtcggt atcgaaggcc
     3241 tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg tactggcacg
     3301 tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac aggcatcata
     3361 cccactcctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac aacgccaagt
     3421 cataccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc ggcacccgcc
     3481 caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt cagcaaggct
     3541 tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca ctgggctgcg
     3601 tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct accaccgatt
     3661 ctatgccccc acttctgaaa caagcaattg agctgttcga ccggcaggga gccgaacctg
     3721 ccttcctttt cggcctggaa ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa
     3781 gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga catgctccca gccgatgccc
     3841 ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt gaccttgaca
     3901 tgctccccgg gtaaacccag ctttcttgta caaagtggtg ataatcgaat tccgataatc
     3961 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     4021 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     4081 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     4141 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     4201 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     4261 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     4321 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     4381 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     4441 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     4501 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcgg gaattcccgc
     4561 ggttcgaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     4621 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     4681 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     4741 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     4801 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     4861 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     4921 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     4981 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     5041 cgctgttctc ctcttcctca tctccgggcc tttcgacctc acgtgcgcat gattgaacaa
     5101 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     5161 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     5221 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     5281 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     5341 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     5401 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     5461 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     5521 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     5581 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     5641 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     5701 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     5761 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     5821 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     5881 tgagcgggac tctgggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     5941 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     6001 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     6061 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     6121 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6181 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     6241 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     6301 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6361 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     6421 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6481 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     6541 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     6601 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     6661 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     6721 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     6781 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     6841 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     6901 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     6961 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     7021 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     7081 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     7141 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     7201 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     7261 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     7321 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     7381 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     7441 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     7501 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     7561 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     7621 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     7681 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     7741 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     7801 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     7861 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     7921 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     7981 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     8041 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     8101 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     8161 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     8221 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     8281 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     8341 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     8401 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     8461 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     8521 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     8581 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     8641 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     8701 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     8761 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaaga gagaaaggcg
     8821 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     8881 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8941 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     9001 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     9061 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     9121 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     9181 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     9241 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     9301 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     9361 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     9421 gaacaaaagc tggagctgca agctt
//
