LOCUS       Exported               11380 bp DNA     circular SYN 22-MAR-2022
DEFINITION  .
ACCESSION   VB190715-1035jad
VERSION     .
KEYWORDS    VB190715-1035jad.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 22, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11380
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="/application_notes=Pol III promoter; drives 
                     expression ofsmall RNAs."
                     /note="/description=Human U6 small nuclear 1 promoter"
                     /note="/full_name=Human U6 promoter"
                     /note="/official_designation=U6"
                     /note="/uuid=0f9979e4676b4788b9441e4543b07317"
                     /note="color: #1f36a9; direction: RIGHT"
     misc_feature    251..346
                     /label={long left}
                     /label=nonstandard type:  gRNA
                     /note="/application_notes="
                     /note="/description=only for mdx cells, targets the 
                     pointmutation"
                     /note="/official_designation={long left}"
                     /note="/uuid=55e883e0786f4540a771744b059d1533"
                     /note="color: #db6901; direction: RIGHT"
     terminator      347..352
                     /label=Terminator
                     /note="/application_notes=Allows transcription termination 
                     ofsmall RNA transcribed by Pol III RNA polymerase."
                     /note="/description=Pol III transcription terminator"
                     /note="/official_designation=Terminator"
                     /note="/uuid=a2ca82806fa441698297d9fb7e458916"
                     /note="color: #800c0c; direction: RIGHT"
     promoter        361..609
                     /label=U6 promoter
                     /note="/application_notes=Pol III promoter; drives 
                     expression ofsmall RNAs."
                     /note="/description=Human U6 small nuclear 1 promoter"
                     /note="/full_name=Human U6 promoter"
                     /note="/official_designation=U6"
                     /note="/uuid=0f9979e4676b4788b9441e4543b07317"
                     /note="color: #0c5d90; direction: RIGHT"
     misc_feature    611..706
                     /label={long right}
                     /label=nonstandard type:  gRNA
                     /note="/application_notes="
                     /note="/official_designation={long right}"
                     /note="/uuid=7e837bbca324464690ac855b10316722"
                     /note="color: #f58600; direction: RIGHT"
     terminator      707..712
                     /label=Terminator
                     /note="/application_notes=Allows transcription termination 
                     ofsmall RNA transcribed by Pol III RNA polymerase."
                     /note="/description=Pol III transcription terminator"
                     /note="/official_designation=Terminator"
                     /note="/uuid=a2ca82806fa441698297d9fb7e458916"
                     /note="color: #5566f5; direction: RIGHT"
     promoter        726..1523
                     /label=CBh promoter
                     /note="/application_notes=Strong promoter."
                     /note="/description=CMV early enhancer fused to modified 
                     chickenbeta-actin promoter"
                     /note="/full_name=The short version of CAG promoter"
                     /note="/official_designation=CBh"
                     /note="/uuid=ebd6140b3e0445c4a59db5b875853bca"
                     /note="color: #ef6cdf; direction: RIGHT"
     misc_feature    1536..5807
                     /label=hCas9
                     /label=nonstandard type:  Nuclease
                     /note="/application_notes=Generates double-strand DNA 
                     breaks."
                     /note="/description=Human codon-optimized CRISPR 
                     associatedprotein 9 from Streptococcus pyogenes (with 
                     3xFLAG tag and nuclear localization signal), also known as 
                     SpCas9"
                     /note="/full_name=Human codon-optimized CRISPR associated 
                     protein9 with 3×FLAG tag and NLS"
                     /note="/official_designation=hCas9"
                     /note="/uuid=8da389c91be543b8b233432ded2ef9af"
                     /note="color: #c54b7c; direction: RIGHT"
     polyA_signal    5838..6045
                     /label=BGH pA
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Bovine growth hormone polyadenylation 
                     signal"
                     /note="/full_name=Bovine growth hormone polyadenylation"
                     /note="/official_designation=BGH pA"
                     /note="/uuid=4a09734ca6c745b3aadc757c1638b651"
                     /note="color: #d05c0a; direction: RIGHT"
     promoter        6057..6644
                     /label=CMV promoter
                     /note="/application_notes=Strong promoter; may have 
                     variablestrength in some cell types."
                     /note="/description=Human cytomegalovirus immediate 
                     earlyenhancer/promoter"
                     /note="/full_name=Human cytomegalovirus immediate early 
                     promoter"
                     /note="/official_designation=CMV"
                     /note="/uuid=5257af780b9a4261b6ff271ec041eff9"
                     /note="color: #fd3434; direction: RIGHT"
     misc_feature    6676..8409
                     /label=mCherry/Hygro
                     /label=nonstandard type:  Marker
                     /note="/application_notes=Allows cells to be visualized by 
                     redfluorescence and resistant to hygromycin B."
                     /note="/description=mCherry fused with Hygro"
                     /note="/full_name=mCherry and hygromycin dual reporter 
                     gene"
                     /note="/official_designation=mCherry/Hygro"
                     /note="/uuid=9bf5b71e7afa4ef8b0748790b8897552"
                     /note="color: #46c6ef; direction: RIGHT"
     polyA_signal    8413..8634
                     /label=SV40 late pA
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Simian virus 40 late polyadenylation 
                     signal"
                     /note="/official_designation=SV40 late pA"
                     /note="/uuid=d23bf533e0924dfcb932fc3ddd77e791"
                     /note="color: #eda481; direction: RIGHT"
     misc_feature    9700..10560
                     /label=Ampicillin
                     /label=nonstandard type:  ORF
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampicillin."
                     /note="/description=Ampicillin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=5bcc09480c0e4c07b95ddccd8242d769"
                     /note="color: #da3bb5; direction: RIGHT"
     rep_origin      10731..11319
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=b978ad4ff785460db448caf63b728237"
                     /note="color: #e4b930"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg tctttgaaag agcaataaaa gttttagagc tagaaatagc aagttaaaat
      301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttggccggcc
      361 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
      421 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      481 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      541 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      601 cgaaacaccg atttcaggta agccgaggtt gttttagagc tagaaatagc aagttaaaat
      661 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgtctagag
      721 gtacccgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      781 ccattgacgt caatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      841 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      901 attgacgtca atgacggtaa atggcccgcc tggcattgtg cccagtacat gaccttatgg
      961 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg
     1021 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat
     1081 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg
     1141 ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca
     1201 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg
     1261 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg
     1321 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
     1381 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagctgag caagaggtaa
     1441 gggtttaagg gatggttggt tggtggggta ttaatgttta attacctgga gcacctgcct
     1501 gaaatcactt tttttcaggt tggaccggtg ccaccatgga ctataaggac cacgacggag
     1561 actacaagga tcatgatatt gattacaaag acgatgacga taagatggcc ccaaagaaga
     1621 agcggaaggt cggtatccac ggagtcccag cagccgacaa gaagtacagc atcggcctgg
     1681 acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca
     1741 agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag
     1801 ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa
     1861 gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga
     1921 tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg
     1981 ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg
     2041 agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg
     2101 acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga
     2161 tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc
     2221 agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg
     2281 ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc
     2341 ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc
     2401 ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca
     2461 cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt
     2521 ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca
     2581 ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc
     2641 aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga
     2701 ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg
     2761 aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc
     2821 tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca
     2881 tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt
     2941 acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct
     3001 actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg
     3061 aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga
     3121 gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca
     3181 agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg
     3241 tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg
     3301 acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca
     3361 agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct
     3421 ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg
     3481 aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag
     3541 agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc
     3601 agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca
     3661 tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca
     3721 acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga
     3781 aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca
     3841 gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag
     3901 tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca
     3961 cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag
     4021 agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga
     4081 agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca
     4141 tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg
     4201 actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg
     4261 tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca
     4321 agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg
     4381 aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc
     4441 acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga
     4501 tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt
     4561 tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga
     4621 acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt
     4681 acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg
     4741 gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga
     4801 ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg
     4861 gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc
     4921 cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta
     4981 tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga
     5041 agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg
     5101 aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg
     5161 aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag
     5221 tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc
     5281 ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct
     5341 ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg
     5401 aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca
     5461 tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag
     5521 tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca
     5581 tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca
     5641 ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc
     5701 agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga ggcgacaaaa
     5761 ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagtaagaa ttcctagagc
     5821 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     5881 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     5941 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     6001 cagcaagggg gaggattggg aagagaatag caggcatgct ggggagcggc cgccgcgttg
     6061 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     6121 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     6181 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     6241 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     6301 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     6361 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     6421 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     6481 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     6541 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     6601 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
     6661 acccactgcg ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag
     6721 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     6781 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     6841 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     6901 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     6961 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     7021 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     7081 tccgacggcc ccgtaatgca gaagaaaacc atgggctggg aggcctcctc cgagcggatg
     7141 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     7201 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     7261 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     7321 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     7381 aagatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     7441 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     7501 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     7561 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     7621 attggggaat ttagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
     7681 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
     7741 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     7801 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     7861 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     7921 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     7981 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     8041 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     8101 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     8161 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     8221 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     8281 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     8341 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     8401 aaggaatagg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     8461 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     8521 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     8581 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtagcggcc
     8641 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     8701 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     8761 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
     8821 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     8881 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg
     8941 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     9001 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     9061 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     9121 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     9181 acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc
     9241 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     9301 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     9361 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     9421 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     9481 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     9541 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     9601 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9661 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     9721 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     9781 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     9841 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     9901 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     9961 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
    10021 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
    10081 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    10141 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    10201 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
    10261 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    10321 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    10381 tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat tgcagcactg
    10441 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    10501 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    10561 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    10621 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    10681 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    10741 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    10801 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    10861 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    10921 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    10981 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    11041 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    11101 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    11161 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    11221 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    11281 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    11341 ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
