LOCUS       Exported               11632 bp DNA     circular SYN 22-MAR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 22, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11632
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="color: #31849b"
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 143 .. 148 / #ffffff / -35
                        2: 149 .. 166 / #ffffff
                        3: 167 .. 173 / #ffffff / -10"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    295..1098
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
                     /note="color: #ffe4c4"
     polyA_signal    1129..1210
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     CDS             complement(1327..2073)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /note="color: #993366"
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(2092..2602)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    2622..2670
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    2684..2775
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
                     /note="color: #a6acb3"
     promoter        2779..3146
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    2787..2805
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2823..2841
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2859..2877
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2895..2913
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2931..2949
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2967..2985
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    3003..3021
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     CDS             3181..3246
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #cc99b2
                     Cleavage site after base 3246"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3265..4194
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /note="color: #00ffff"
                     /translation="TSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     protein_bind    4222..4246
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    4267..5491
                     /label=beta-globin
                     /note="color: #cc99ff"
     intron          4356..4485
                     /label=intron 1
                     /note="color: #------"
     misc_feature    4511..4513
                     /label=Converted to TAG in NMD(+) PTC39 reporter version
                     /note="color: #ff0000"
     intron          4709..5362
                     /label=intron 2
                     /note="color: #------"
     polyA_signal    5649..5730
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        5752..6262
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6284..6883
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    6890..7114
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    7128..7964
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
                     /note="color: #ffe4c4"
     promoter        complement(8005..8023)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(8030..8046)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     CDS             8184..8486
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be
                     propagated in standard E. coli strains."
                     /note="color: #993366"
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             8835..9629
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(9879..10739)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  9879 .. 10670 / #ccffcc
                        2: 10671 .. 10739 / #ccffcc / signal sequence
                     Cleavage site after base 10670"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10863..11451
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc cctttgcttt
      301 ctctgaccag cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg
      361 ggtcacctct acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct
      421 ccactccctc ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc
      481 acttccttct cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg
      541 tcctctccgg gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc
      601 ccttttcctt ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg
      661 acggatgtct cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt
      721 ctggacaacc ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt
      781 tctttgcctg gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg
      841 ggcagctccc ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca
      901 tggcatcttc caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat
      961 gtccacttca ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc
     1021 accttatatt cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc
     1081 tccaccccac agtggggcaa gctgagtcag tgagcgagga agctcggggc agtgaaaaaa
     1141 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     1201 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     1261 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct
     1321 ggagatttac ccggggagca tgtcaaggtc aaaatcgtca agagcgtcag caggcagcat
     1381 atcaaggtca aagtcgtcaa gggcatcggc tgggagcatg tctaagtcaa aatcgtcaag
     1441 ggcgtcggtc ggcccgccgc tttcgcactt tagctgtttc tccaggccac atatgattag
     1501 ttccaggccg aaaaggaagg caggttcggc tccctgccgg tcgaacagct caattgcttg
     1561 tttcagaagt gggggcatag aatcggtggt aggtgtctct ctttcctctt ttgctacttg
     1621 atgctcctgt tcctccaata cgcagcccag tgtaaagtgg cccacggcgg acagagcgta
     1681 cagtgcgttc tccagggaga agccttgctg acacaggaac gcgagctgat tttccagggt
     1741 ttcgtactgt ttctctgttg ggcgggtgcc gagatgcact ttagccccgt cgcgatgtga
     1801 gaggagagca cagcggtatg acttggcgtt gttccgcaga aagtcttgcc atgactcgcc
     1861 ttccaggggg caggagtggg tatgatgcct gtccagcatc tcgattggca gggcatcgag
     1921 cagggcccgc ttgttcttca cgtgccagta cagggtaggc tgctcaactc ccagcttttg
     1981 agcgagtttc cttgtcgtca ggccttcgat accgactcca ttgagtaatt ccagagcaga
     2041 gtttatgact ttgctcttgt ccagtctaga catggtaatt cgatgatccc cctggggaga
     2101 gaggtcggtg attcggtcaa cgagggagcc gactgccgac gtgcgctccg gaggcttgca
     2161 gaatgcggaa caccgcgcgg gcaggaacag ggcccacact accgccccac accccgcctc
     2221 ccgcaccgcc ccttcccggc cgctgctctc ggcgcgccct gctgagcagc cgctattggc
     2281 cacagcccat cgcggtcggc gcgctgccat tgctccctgg cgctgtccgt ctgcgagggt
     2341 actagtgaga cgtgcggctt ccgtttgtca cgtccggcac gccgcgaacc gcaaggaacc
     2401 ttcccgactt aggggcggag caggaagcgt cgccgggggg cccacaaggg tagcggcgaa
     2461 gatccgggtg acgctgcgaa cggacgtgaa gaatgtgcga gacccagggt cggcgccgct
     2521 gcgtttcccg gaaccacgcc cagagcagcc gcgtccctgc gcaaacccag ggctgccttg
     2581 gaaaaggcgc aaccccaacc ccgtggaatt atcacctcga gaataaaata tctttatttt
     2641 cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc
     2701 aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg
     2761 ccagaacatt tctctctcga gtttactccc tatcagtgat agagaacgta tgaagagttt
     2821 actccctatc agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg
     2881 tataaggagt ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag
     2941 tgatagagaa cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt
     3001 actccctatc agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa
     3061 aagagtgctg attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt
     3121 tccgtaccac ttcctaccct cgtaaagaat tcgcggccgc aaccgtcaga attaaccatg
     3181 gactacaaag accatgacgg tgattataaa gatcatgaca tcgattacaa ggatgacgat
     3241 gacaagcttg cggccgcgaa ttcaacttcg aaagtttatg atccagaaca aaggaaacgg
     3301 atgataactg gtccgcagtg gtgggccaga tgtaaacaaa tgaatgttct tgattcattt
     3361 attaattatt atgattcaga aaaacatgca gaaaatgctg ttattttttt acatggtaac
     3421 gcggcctctt cttatttatg gcgacatgtt gtgccacata ttgagccagt agcgcggtgt
     3481 attataccag accttattgg tatgggcaaa tcaggcaaat ctggtaatgg ttcttatagg
     3541 ttacttgatc attacaaata tcttactgca tggtttgaac ttcttaattt accaaagaag
     3601 atcatttttg tcggccatga ttggggtgct tgtttggcat ttcattatag ctatgagcat
     3661 caagataaga tcaaagcaat agttcacgct gaaagtgtag tagatgtgat tgaatcatgg
     3721 gatgaatggc ctgatattga agaagatatt gcgttgatca aatctgaaga aggagaaaaa
     3781 atggttttgg agaataactt cttcgtggaa accatgttgc catcaaaaat catgagaaag
     3841 ttagaaccag aagaatttgc agcatatctt gaaccattca aagagaaagg tgaagttcgt
     3901 cgtccaacat tatcatggcc tcgtgaaatc ccgttagtaa aaggtggtaa acctgacgtt
     3961 gtacaaattg ttaggaatta taatgcttat ctacgtgcaa gtgatgattt accaaaaatg
     4021 tttattgaat cggacccagg attcttttcc aatgctattg ttgaaggtgc caagaagttt
     4081 cctaatactg aatttgtcaa agtaaaaggt cttcattttt cgcaagaaga tgcacctgat
     4141 gaaatgggaa aatatatcaa atcgttcgtt gagcgagttc tcaaaaatga acaagcgaat
     4201 tcatcgatag atctgatatc aacaagtttg tacaaaaaag caggctccgc ggccgccccc
     4261 ttcaccgtgc atctgactcc tgaggagaag tctgccgtta ctgccctgtg gggcaaggtg
     4321 aacgtggatg aagttggtgg tgaggccctg ggcaggttgg tatcaaggtt acaagacagg
     4381 tttaaggaga ccaatagaaa ctgggcatgt ggagacagag aagactcttg ggtttctgat
     4441 aggcactgac tctctctgcc tattggtcta ttttcccacc cttaggctgc tggtggtcta
     4501 cccttggacc cagaggttct ttgagtcctt tggggatctg tccactcctg atgctgttat
     4561 gggcaaccct aaggtgaagg ctcatggcaa gaaagtgctc ggtgccttta gtgatggcct
     4621 ggctcacctg gacaacctca agggcacctt tgccacactg agtgagctgc actgtgacaa
     4681 gctgcacgtg gatcctgaga acttcagggt gagtctgatg ggcacctcct gggtttcctt
     4741 cccctggcta ttctgctcaa ccttcctatc agaaaaaaag gggaagcgat tctagggagc
     4801 agtctccatg actgtgtgtg gagtgttgac aagagttcgg atattttatt ctctactcag
     4861 aattgctgct ccccctcact ctgttctgtg ttgtcatttc ctctttcttt ggtaagcttt
     4921 taatttccag ttgcatttta ctaaattaat taagctggtt atttacttcc catcctgata
     4981 tcagcttccc ctcctccttt cctcccagtc cttctctctc tcctctctct ttctctaatc
     5041 ctttcctttc cctcagttca tttcttcttc tttgatctac gtttgtttgt ctttttaaat
     5101 attgccttgt aacttgctca gaggacaagg aagatatgtc cctgtttctt ctcatagctc
     5161 tcaagaatag tagcataatt ggcttttatg ccagggtgac aggggaagaa tatattttac
     5221 atataaattc tgtttgacat aggattctta taataatttg tcagtagttt aaggttgcaa
     5281 acaaatgtct ttgtaaataa gcctgcagta tctggtattt ttgctctaca gttatgttga
     5341 tggttcttcc atattcccac agctcctggg caatatgatc gtgattgtgc tgggccacca
     5401 cctgggcaag gatttcaccc ccgctgcaca ggctgccttc cagaaggtgg tggctggagt
     5461 ggccactgcc ctggcccaca agtatcacta aaagggtggg cgcgccgacc cagctttctt
     5521 gttctgcagc atatgtctag aggatcataa tcagccatac cacatttgta gaggttttac
     5581 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     5641 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5701 atttcacaaa taaagcattt ttttcactgc cgacgtcagg tggcactttt cggggttggg
     5761 gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg
     5821 ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag
     5881 cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc
     5941 cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc
     6001 acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc
     6061 gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga
     6121 aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg
     6181 gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg
     6241 aatcaccgac ctctctcccc agggggatcc accggagctt accatgaccg agtacaagcc
     6301 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     6361 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     6421 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     6481 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     6541 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     6601 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     6661 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     6721 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     6781 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     6841 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaggtaccc tgtgccttct
     6901 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     6961 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     7021 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     7081 agcaggcatg ctggggatgc ggtgggctct atggggtacc aagctttact agggacagga
     7141 ttggtgacag aaaagcccca tccttaggcc tcctccttcc tagtctcctg atattgggtc
     7201 taacccccac ctcctgttag gcagattcct tatctggtga cacaccccca tttcctggag
     7261 ccatctctct ccttgccaga acctctaagg tttgcttacg atggagccag agaggatcct
     7321 gggagggaga gcttggcagg gggtgggagg gaaggggggg atgcgtgacc tgcccggttc
     7381 tcagtggcca ccctgcgcta ccctctccca gaacctgagc tgctctgacg cggctgtctg
     7441 gtgcgtttca ctgatcctgg tgctgcagct tccttacact tcccaagagg agaagcagtt
     7501 tggaaaaaca aaatcagaat aagttggtcc tgagttctaa ctttggctct tcacctttct
     7561 agtccccaat ttatattgtt cctccgtgcg tcagttttac ctgtgagata aggccagtag
     7621 ccagccccgt cctggcaggg ctgtggtgag gaggggggtg tccgtgtgga aaactccctt
     7681 tgtgagaatg gtgcgtccta ggtgttcacc aggtcgtggc cgcctctact ccctttctct
     7741 ttctccatcc ttctttcctt aaagagtccc cagtgctatc tgggacatat tcctccgccc
     7801 agagcagggt cccgcttccc taaggccctg ctctgggctt ctgggtttga gtccttggca
     7861 agcccaggag aggcgctcag gcttccctgt cccccttcct cgtccaccat ctcatgcccc
     7921 tggctctcct gccccttccc tacaggggtt cctggctctg ctctaagggc aagggcgaat
     7981 tcgcggccgc taaattcaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg
     8041 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     8101 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     8161 agttgcgcag cctatacgta cggcagttta aggtttacac ctataaaaga gagagccgtt
     8221 atcgtctgtt tgtggatgta cagagtgata ttattgacac gccggggcga cggatggtga
     8281 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
     8341 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
     8401 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     8461 ttaacctgat gttctgggga atataaatgt caggcatgag attatcaaaa aggatcttca
     8521 cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg
     8581 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt
     8641 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg
     8701 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
     8761 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat
     8821 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     8881 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     8941 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     9001 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     9061 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     9121 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     9181 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     9241 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     9301 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     9361 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     9421 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     9481 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     9541 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     9601 tctatcgcct tcttgacgag ttcttctgaa ttattaacgc ttacaatttc ctgatgcggt
     9661 attttctcct tacgcatctg tgcggtattt cacaccgcat caggtggcac ttttcgggga
     9721 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     9781 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     9841 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     9901 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     9961 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    10021 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    10081 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    10141 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    10201 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    10261 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    10321 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    10381 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    10441 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    10501 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    10561 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    10621 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    10681 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    10741 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gaccaaaatc
    10801 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    10861 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    10921 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    10981 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    11041 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    11101 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    11161 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    11221 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    11281 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    11341 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    11401 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    11461 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    11521 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    11581 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga ag
//
