LOCUS       Exported               12349 bp DNA     circular SYN 22-MAR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 22, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 143 .. 148 / #ffffff / -35
                        2: 149 .. 166 / #ffffff
                        3: 167 .. 173 / #ffffff / -10"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    295..1098
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
                     /note="color: #ffe4c4"
     polyA_signal    1129..1210
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     CDS             complement(1327..2073)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /note="color: #993366"
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(2092..2602)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    2622..2670
                     /note="synthetic polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    2684..2775
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
                     /note="color: #a6acb3"
     promoter        2779..3146
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    2787..2805
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2823..2841
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2859..2877
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2895..2913
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2931..2949
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2967..2985
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    3003..3021
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     CDS             3181..3246
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #cc99b2
                     Cleavage site after base 3246"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3265..4911
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /note="color: #ffcc00"
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     protein_bind    4939..4963
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    4984..6208
                     /label=beta-globin
                     /note="color: #cc99ff"
     intron          5073..5202
                     /label=intron 1
                     /note="color: #------"
     misc_feature    5228..5230
                     /label=PTC39
                     /note="color: #ff0000"
     intron          5426..6079
                     /label=intron 2
                     /note="color: #------"
     polyA_signal    6366..6447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        6469..6979
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             7001..7600
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    7607..7831
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    7845..8681
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
                     /note="color: #ffe4c4"
     promoter        complement(8722..8740)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(8747..8763)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     CDS             8901..9203
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /note="color: #993366"
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             9552..10346
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(10596..11456)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 10596 .. 11387 / #ccffcc
                        2: 11388 .. 11456 / #ccffcc / signal sequence
                     Cleavage site after base 11387"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11580..12168
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc cctttgcttt
      301 ctctgaccag cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg
      361 ggtcacctct acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct
      421 ccactccctc ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc
      481 acttccttct cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg
      541 tcctctccgg gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc
      601 ccttttcctt ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg
      661 acggatgtct cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt
      721 ctggacaacc ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt
      781 tctttgcctg gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg
      841 ggcagctccc ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca
      901 tggcatcttc caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat
      961 gtccacttca ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc
     1021 accttatatt cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc
     1081 tccaccccac agtggggcaa gctgagtcag tgagcgagga agctcggggc agtgaaaaaa
     1141 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     1201 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     1261 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct
     1321 ggagatttac ccggggagca tgtcaaggtc aaaatcgtca agagcgtcag caggcagcat
     1381 atcaaggtca aagtcgtcaa gggcatcggc tgggagcatg tctaagtcaa aatcgtcaag
     1441 ggcgtcggtc ggcccgccgc tttcgcactt tagctgtttc tccaggccac atatgattag
     1501 ttccaggccg aaaaggaagg caggttcggc tccctgccgg tcgaacagct caattgcttg
     1561 tttcagaagt gggggcatag aatcggtggt aggtgtctct ctttcctctt ttgctacttg
     1621 atgctcctgt tcctccaata cgcagcccag tgtaaagtgg cccacggcgg acagagcgta
     1681 cagtgcgttc tccagggaga agccttgctg acacaggaac gcgagctgat tttccagggt
     1741 ttcgtactgt ttctctgttg ggcgggtgcc gagatgcact ttagccccgt cgcgatgtga
     1801 gaggagagca cagcggtatg acttggcgtt gttccgcaga aagtcttgcc atgactcgcc
     1861 ttccaggggg caggagtggg tatgatgcct gtccagcatc tcgattggca gggcatcgag
     1921 cagggcccgc ttgttcttca cgtgccagta cagggtaggc tgctcaactc ccagcttttg
     1981 agcgagtttc cttgtcgtca ggccttcgat accgactcca ttgagtaatt ccagagcaga
     2041 gtttatgact ttgctcttgt ccagtctaga catggtaatt cgatgatccc cctggggaga
     2101 gaggtcggtg attcggtcaa cgagggagcc gactgccgac gtgcgctccg gaggcttgca
     2161 gaatgcggaa caccgcgcgg gcaggaacag ggcccacact accgccccac accccgcctc
     2221 ccgcaccgcc ccttcccggc cgctgctctc ggcgcgccct gctgagcagc cgctattggc
     2281 cacagcccat cgcggtcggc gcgctgccat tgctccctgg cgctgtccgt ctgcgagggt
     2341 actagtgaga cgtgcggctt ccgtttgtca cgtccggcac gccgcgaacc gcaaggaacc
     2401 ttcccgactt aggggcggag caggaagcgt cgccgggggg cccacaaggg tagcggcgaa
     2461 gatccgggtg acgctgcgaa cggacgtgaa gaatgtgcga gacccagggt cggcgccgct
     2521 gcgtttcccg gaaccacgcc cagagcagcc gcgtccctgc gcaaacccag ggctgccttg
     2581 gaaaaggcgc aaccccaacc ccgtggaatt atcacctcga gaataaaata tctttatttt
     2641 cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc
     2701 aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg
     2761 ccagaacatt tctctctcga gtttactccc tatcagtgat agagaacgta tgaagagttt
     2821 actccctatc agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg
     2881 tataaggagt ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag
     2941 tgatagagaa cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt
     3001 actccctatc agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa
     3061 aagagtgctg attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt
     3121 tccgtaccac ttcctaccct cgtaaagaat tcgcggccgc aaccgtcaga attaaccatg
     3181 gactacaaag accatgacgg tgattataaa gatcatgaca tcgattacaa ggatgacgat
     3241 gacaagcttg cggccgcgaa ttcagaagac gccaaaaaca taaagaaagg cccggcgcca
     3301 ttctatccgc tggaagatgg aaccgctgga gagcaactgc ataaggctat gaagagatac
     3361 gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgga catcacttac
     3421 gctgagtact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat
     3481 acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg
     3541 ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa
     3601 ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg
     3661 caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat tatcatggat
     3721 tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct
     3781 cccggtttta atgaatacga ttttgtgcca gagtccttcg atagggacaa gacaattgca
     3841 ctgatcatga actcctctgg atctactggt ctgcctaaag gtgtcgctct gcctcataga
     3901 actgcctgcg tgagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg
     3961 gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc
     4021 ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt
     4081 ctgaggagcc ttcaggatta caagattcaa agtgcgctgc tggtgccaac cctattctcc
     4141 ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct
     4201 tctggtggcg ctcccctctc taaggaagtc ggggaagcgg ttgccaagag gttccatctg
     4261 ccaggtatca ggcaaggata tgggctcact gagactacat cagctattct gattacaccc
     4321 gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt
     4381 gtggatctgg ataccgggaa aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga
     4441 ggtcctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac
     4501 aaggatggat ggctacattc tggagacata gcttactggg acgaagacga acacttcttc
     4561 atcgttgacc gcctgaagtc tctgattaag tacaaaggct atcaggtggc tcccgctgaa
     4621 ttggaatcca tcttgctcca acaccccaac atcttcgacg caggtgtcgc aggtcttccc
     4681 gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg
     4741 acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc
     4801 ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga
     4861 aaaatcagag agatcctcat aaaggccaag aagggcggaa agatcgccgt ggcgaattca
     4921 tcgatagatc tgatatcaac aagtttgtac aaaaaagcag gctccgcggc cgcccccttc
     4981 accgtgcatc tgactcctga ggagaagtct gccgttactg ccctgtgggg caaggtgaac
     5041 gtggatgaag ttggtggtga ggccctgggc aggttggtat caaggttaca agacaggttt
     5101 aaggagacca atagaaactg ggcatgtgga gacagagaag actcttgggt ttctgatagg
     5161 cactgactct ctctgcctat tggtctattt tcccaccctt aggctgctgg tggtctaccc
     5221 ttggacctag aggttctttg agtcctttgg ggatctgtcc actcctgatg ctgttatggg
     5281 caaccctaag gtgaaggctc atggcaagaa agtgctcggt gcctttagtg atggcctggc
     5341 tcacctggac aacctcaagg gcacctttgc cacactgagt gagctgcact gtgacaagct
     5401 gcacgtggat cctgagaact tcagggtgag tctgatgggc acctcctggg tttccttccc
     5461 ctggctattc tgctcaacct tcctatcaga aaaaaagggg aagcgattct agggagcagt
     5521 ctccatgact gtgtgtggag tgttgacaag agttcggata ttttattctc tactcagaat
     5581 tgctgctccc cctcactctg ttctgtgttg tcatttcctc tttctttggt aagcttttaa
     5641 tttccagttg cattttacta aattaattaa gctggttatt tacttcccat cctgatatca
     5701 gcttcccctc ctcctttcct cccagtcctt ctctctctcc tctctctttc tctaatcctt
     5761 tcctttccct cagttcattt cttcttcttt gatctacgtt tgtttgtctt tttaaatatt
     5821 gccttgtaac ttgctcagag gacaaggaag atatgtccct gtttcttctc atagctctca
     5881 agaatagtag cataattggc ttttatgcca gggtgacagg ggaagaatat attttacata
     5941 taaattctgt ttgacatagg attcttataa taatttgtca gtagtttaag gttgcaaaca
     6001 aatgtctttg taaataagcc tgcagtatct ggtatttttg ctctacagtt atgttgatgg
     6061 ttcttccata ttcccacagc tcctgggcaa tatgatcgtg attgtgctgg gccaccacct
     6121 gggcaaggat ttcacccccg ctgcacaggc tgccttccag aaggtggtgg ctggagtggc
     6181 cactgccctg gcccacaagt atcactaaaa gggtgggcgc gccgacccag ctttcttgtt
     6241 ctgcagcata tgtctagagg atcataatca gccataccac atttgtagag gttttacttg
     6301 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     6361 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6421 tcacaaataa agcatttttt tcactgccga cgtcaggtgg cacttttcgg ggttggggtt
     6481 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
     6541 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
     6601 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
     6661 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
     6721 tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
     6781 atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg
     6841 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
     6901 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
     6961 caccgacctc tctccccagg gggatccacc ggagcttacc atgaccgagt acaagcccac
     7021 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     7081 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     7141 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     7201 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     7261 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     7321 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     7381 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     7441 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     7501 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     7561 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga ggtaccctgt gccttctagt
     7621 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     7681 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     7741 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     7801 aggcatgctg gggatgcggt gggctctatg gggtaccaag ctttactagg gacaggattg
     7861 gtgacagaaa agccccatcc ttaggcctcc tccttcctag tctcctgata ttgggtctaa
     7921 cccccacctc ctgttaggca gattccttat ctggtgacac acccccattt cctggagcca
     7981 tctctctcct tgccagaacc tctaaggttt gcttacgatg gagccagaga ggatcctggg
     8041 agggagagct tggcaggggg tgggagggaa gggggggatg cgtgacctgc ccggttctca
     8101 gtggccaccc tgcgctaccc tctcccagaa cctgagctgc tctgacgcgg ctgtctggtg
     8161 cgtttcactg atcctggtgc tgcagcttcc ttacacttcc caagaggaga agcagtttgg
     8221 aaaaacaaaa tcagaataag ttggtcctga gttctaactt tggctcttca cctttctagt
     8281 ccccaattta tattgttcct ccgtgcgtca gttttacctg tgagataagg ccagtagcca
     8341 gccccgtcct ggcagggctg tggtgaggag gggggtgtcc gtgtggaaaa ctccctttgt
     8401 gagaatggtg cgtcctaggt gttcaccagg tcgtggccgc ctctactccc tttctctttc
     8461 tccatccttc tttccttaaa gagtccccag tgctatctgg gacatattcc tccgcccaga
     8521 gcagggtccc gcttccctaa ggccctgctc tgggcttctg ggtttgagtc cttggcaagc
     8581 ccaggagagg cgctcaggct tccctgtccc ccttcctcgt ccaccatctc atgcccctgg
     8641 ctctcctgcc ccttccctac aggggttcct ggctctgctc taagggcaag ggcgaattcg
     8701 cggccgctaa attcaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
     8761 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     8821 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     8881 tgcgcagcct atacgtacgg cagtttaagg tttacaccta taaaagagag agccgttatc
     8941 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc ggggcgacgg atggtgatcc
     9001 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     9061 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     9121 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     9181 acctgatgtt ctggggaata taaatgtcag gcatgagatt atcaaaaagg atcttcacct
     9241 agatcctttt cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca
     9301 gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca
     9361 gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat
     9421 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     9481 tcttgccgcc aaggatctga tggcgcaggg gatcaagctc tgatcaagag acaggatgag
     9541 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     9601 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     9661 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     9721 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     9781 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     9841 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     9901 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     9961 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
    10021 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
    10081 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
    10141 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
    10201 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
    10261 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
    10321 atcgccttct tgacgagttc ttctgaatta ttaacgctta caatttcctg atgcggtatt
    10381 ttctccttac gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat
    10441 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    10501 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    10561 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    10621 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    10681 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    10741 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    10801 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    10861 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    10921 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    10981 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    11041 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    11101 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    11161 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    11221 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    11281 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    11341 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    11401 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    11461 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgac caaaatccct
    11521 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    11581 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    11641 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    11701 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    11761 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    11821 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    11881 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    11941 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    12001 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    12061 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    12121 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    12181 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    12241 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    12301 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//
