LOCUS       Exported                4755 bp ds-DNA     circular SYN 17-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS     pCS2+/C-SNAPf (codon optimized for Xenopus)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 17, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          119..661
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="/locus_tag=source:synthetic DNA construct"
     misc_feature    28..33
                     /locus_tag="HindIII"
                     /label=HindIII
     primer_bind     35..52
                     /locus_tag="SP6"
                     /label=SP6
     misc_feature    94..99
                     /locus_tag="Primer S"
                     /label=Primer S
     misc_feature    107
                     /locus_tag="Primer AS"
                     /label=Primer AS
     misc_feature    108..112
                     /locus_tag="Xho1(1)"
                     /label=Xho1
                     /label=Xho1(1)
     misc_feature    113..118
                     /locus_tag="XbaI"
                     /label=XbaI
     misc_feature    113
                     /locus_tag="Xho1"
                     /label=Xho1
     CDS             119..661
                     /codon_start=1
                     /locus_tag="SNAP-tag(R)"
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAPf-tag
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
     misc_feature    666..678
                     /locus_tag="PCS2+"
                     /label=PCS2+
     polyA_signal    684..875
                     /locus_tag="SV40 late polyA"
                     /label=SV40 late polyA
     primer_bind     complement(925..944)
                     /locus_tag="T3"
                     /label=T3
     primer_bind     complement(962..982)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_binding    complement(988..1010)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(1350..2032)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     CDS             complement(2130..2789)
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      complement(3250..3556)
                     /direction=LEFT
                     /locus_tag="F1 ori"
                     /label=F1 ori
     CDS             complement(3575..3643)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattca aggcctctcg agtctagaga
      121 caaagactgc gaaatgaagc gcaccaccct ggatagccct ctgggcaagc tggaactgtc
      181 tgggtgcgaa cagggcctgc accgtatcat cttcctgggc aaaggaacat ctgccgccga
      241 cgccgtggaa gtgcctgccc cagccgccgt gctgggcgga ccagagccac tgatgcaggc
      301 caccgcctgg ctcaacgcct actttcacca gcctgaggcc atcgaggagt tccctgtgcc
      361 agccctgcac cacccagtgt tccagcagga gagctttacc cgccaggtgc tgtggaaact
      421 gctgaaagtg gtgaagttcg gagaggtcat cagctacagc cacctggccg ccctggccgg
      481 caatcccgcc gccaccgccg ccgtgaaaac cgccctgagc ggaaatcccg tgcccattct
      541 gatcccctgc cacagagtgg tgcagggcga cctggacgtg gggggctacg agggcgggct
      601 cgccgtgaaa gagtggctgc tggcccacga gggccacaga ctgggcaagc ctgggctggg
      661 ttaatgtaca aggttacgag atccagacat gataagatac attgatgagt ttggacaaac
      721 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
      781 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
      841 gtttcaggtt cagggggagg tgtgggaggt tttttaattc gcggccgcgg cgccaatgca
      901 ttgggcccgg tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
      961 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     1021 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     1081 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1141 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1201 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1261 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1321 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1381 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1441 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1501 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1561 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1621 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1681 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1741 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1801 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1861 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1921 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1981 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2041 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2101 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2161 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2221 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2281 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2341 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2401 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2461 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2521 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2581 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2641 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2701 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2761 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2821 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2881 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2941 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3001 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3061 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     3121 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     3181 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     3241 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     3301 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     3361 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     3421 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     3481 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     3541 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
     3601 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagtcgacc atagccaatt
     3661 caatatggcg tatatggact catgccaatt caatatggtg gatctggacc tgtgccaatt
     3721 caatatggcg tatatggact cgtgccaatt caatatggtg gatctggacc ccagccaatt
     3781 caatatggcg gacttggcac catgccaatt caatatggcg gacttggcac tgtgccaact
     3841 ggggaggggt ctacttggca cggtgccaag tttgaggagg ggtcttggcc ctgtgccaag
     3901 tccgccatat tgaattggca tggtgccaat aatggcggcc atattggcta tatgccagga
     3961 tcaatatata ggcaatatcc aatatggccc tatgccaata tggctattgg ccaggttcaa
     4021 tactatgtat tggccctatg ccatatagta ttccatatat gggttttcct attgacgtag
     4081 atagcccctc ccaatgggcg gtcccatata ccatatatgg ggcttcctaa taccgcccat
     4141 agccactccc ccattgacgt caatggtctc tatatatggt ctttcctatt gacgtcatat
     4201 gggcggtcct attgacgtat atggcgcctc ccccattgac gtcaattacg gtaaatggcc
     4261 cgcctggctc aatgcccatt gacgtcaata ggaccaccca ccattgacgt caatgggatg
     4321 gctcattgcc cattcatatc cgttctcacg ccccctattg acgtcaatga cggtaaatgg
     4381 cccacttggc agtacatcaa tatctattaa tagtaacttg gcaagtacat tactattgga
     4441 aggacgccag ggtacattgg cagtactccc attgacgtca atggcggtaa atggcccgcg
     4501 atggctgcca agtacatccc cattgacgtc aatggggagg ggcaatgacg caaatgggcg
     4561 ttccattgac gtaaatgggc ggtaggcgtg cctaatggga ggtctatata agcaatgctc
     4621 gtttagggaa ccctggtnct actcacttct ccttctctac tnactaactc tntctagctt
     4681 gctacctnct ctaactaccc tncttctcac ctcctnctaa cagngctnac tactcctnct
     4741 caatgtacct caagt
//
