LOCUS       Exported                5126 bp ds-DNA     circular SYN 22-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCS2+/N-Halotag (codon optimized for Xenopus)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 22, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..16
                     /locus_tag="pCS2 S"
                     /label=pCS2 S
     primer_bind     35..52
                     /locus_tag="SP6"
                     /label=SP6
     misc_feature    87..89
                     /locus_tag="Xen Kozak Sequence"
                     /label=Xen Kozak Sequence
     misc_feature    90..980
                     /locus_tag="Halotag"
                     /label=Halotag
     polyA_signal    1055..1246
                     /locus_tag="SV40 late polyA"
                     /label=SV40 late polyA
     primer_bind     complement(1296..1315)
                     /locus_tag="T3"
                     /label=T3
     misc_feature    1321..1343
                     /locus_tag="pCS2 AS"
                     /label=pCS2 AS
     primer_bind     complement(1333..1353)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_binding    complement(1359..1381)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(1721..2403)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     CDS             complement(2501..3160)
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      complement(3621..3927)
                     /direction=LEFT
                     /locus_tag="F1 ori"
                     /label=F1 ori
     CDS             complement(3946..4014)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atccaaacca tggcagaaat cggtactggc tttccattcg
      121 acccccatta tgtggaagtc ctgggcgagc gcatgcacta cgtcgatgtt ggtccacgcg
      181 atggcacccc tgtgctgttc ctgcacggta acccaacctc ctcctacgtg tggcgcaaca
      241 tcatcccaca tgttgcacca acccatcgct gcattgctcc agacctgatc ggtatgggca
      301 aatccgacaa accagacctg ggttatttct tcgacgacca cgtccgcttc atggatgcct
      361 tcatcgaagc cctgggtctg gaagaggtcg tcctggtcat tcacgactgg ggctccgctc
      421 tgggtttcca ctgggccaag cgcaatccag agcgcgtcaa aggtattgca tttatggagt
      481 tcatccgccc tatcccaacc tgggacgaat ggccagaatt tgcccgcgag accttccagg
      541 ccttccgcac caccgacgtc ggccgcaagc tgatcatcga tcagaacgtt tttatcgagg
      601 gtacactgcc aatgggtgtc gtccgcccac tgactgaagt cgagatggac cattaccgcg
      661 agccattcct gaatcctgtt gaccgcgagc cactgtggcg cttcccaaac gagctgccaa
      721 tcgccggtga gccagctaac atcgtcgctc tggtcgaaga atacatggac tggctgcacc
      781 agtcccctgt cccaaagctg ctgttctggg gcaccccagg cgttctgatc ccaccagccg
      841 aagccgctcg cctggccaaa agcctgccta actgcaaggc tgtggacatc ggcccaggtc
      901 tgaatctgct gcaagaagac aacccagacc tgatcggcag cgagatcgct cgctggctga
      961 gcacactcga gatttccggc tccggactca gatctcgagc tcaagcttcg aattctgcag
     1021 tctagaacta ttagtgagtc gtattacgta gatccagaca tgataagata cattgatgag
     1081 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     1141 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     1201 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt cgcggccgcg
     1261 gcgccaatgc attgggcccg gtacccagct tttgttccct ttagtgaggg ttaattgcgc
     1321 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     1381 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     1441 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1501 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1561 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1621 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1681 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1741 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1801 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1861 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1921 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1981 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2041 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2101 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2161 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2221 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2281 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2341 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2401 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2461 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2521 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2581 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2641 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2701 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2761 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2821 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2881 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2941 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3001 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3061 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3121 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3181 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3241 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3301 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3361 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3421 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3481 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     3541 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     3601 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     3661 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     3721 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     3781 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     3841 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     3901 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     3961 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagtcgac
     4021 catagccaat tcaatatggc gtatatggac tcatgccaat tcaatatggt ggatctggac
     4081 ctgtgccaat tcaatatggc gtatatggac tcgtgccaat tcaatatggt ggatctggac
     4141 cccagccaat tcaatatggc ggacttggca ccatgccaat tcaatatggc ggacttggca
     4201 ctgtgccaac tggggagggg tctacttggc acggtgccaa gtttgaggag gggtcttggc
     4261 cctgtgccaa gtccgccata ttgaattggc atggtgccaa taatggcggc catattggct
     4321 atatgccagg atcaatatat aggcaatatc caatatggcc ctatgccaat atggctattg
     4381 gccaggttca atactatgta ttggccctat gccatatagt attccatata tgggttttcc
     4441 tattgacgta gatagcccct cccaatgggc ggtcccatat accatatatg gggcttccta
     4501 ataccgccca tagccactcc cccattgacg tcaatggtct ctatatatgg tctttcctat
     4561 tgacgtcata tgggcggtcc tattgacgta tatggcgcct cccccattga cgtcaattac
     4621 ggtaaatggc ccgcctggct caatgcccat tgacgtcaat aggaccaccc accattgacg
     4681 tcaatgggat ggctcattgc ccattcatat ccgttctcac gccccctatt gacgtcaatg
     4741 acggtaaatg gcccacttgg cagtacatca atatctatta atagtaactt ggcaagtaca
     4801 ttactattgg aaggacgcca gggtacattg gcagtactcc cattgacgtc aatggcggta
     4861 aatggcccgc gatggctgcc aagtacatcc ccattgacgt caatggggag gggcaatgac
     4921 gcaaatgggc gttccattga cgtaaatggg cggtaggcgt gcctaatggg aggtctatat
     4981 aagcaatgct cgtttaggga accctggtnc tactcacttc tccttctcta ctnactaact
     5041 ctntctagct tgctacctnc tctaactacc ctncttctca cctcctncta acagngctna
     5101 ctactcctnc tcaatgtacc tcaagt
//
