LOCUS       Exported                5100 bp ds-DNA     circular SYN 22-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCS2+/C-Halotag (Codon optimized for Xenopus)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 22, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    28..33
                     /locus_tag="HindIII"
                     /label=HindIII
     primer_bind     35..52
                     /locus_tag="SP6"
                     /label=SP6
     misc_feature    94..99
                     /locus_tag="Primer S"
                     /label=Primer S
     misc_feature    107
                     /locus_tag="Primer AS"
                     /label=Primer AS
     misc_feature    108..112
                     /locus_tag="Xho1(1)"
                     /label=Xho1
                     /label=Xho1(1)
     misc_feature    113..118
                     /locus_tag="XbaI"
                     /label=XbaI
     misc_feature    113
                     /locus_tag="Xho1"
                     /label=Xho1
     misc_feature    119..1006
                     /locus_tag="Halotag"
                     /label=Halotag
     misc_feature    1007..1009
                     /locus_tag="STOP Codon"
                     /label=STOP Codon
     misc_feature    1011..1023
                     /locus_tag="PCS2+"
                     /label=PCS2+
     polyA_signal    1029..1220
                     /locus_tag="SV40 late polyA"
                     /label=SV40 late polyA
     primer_bind     complement(1270..1289)
                     /locus_tag="T3"
                     /label=T3
     primer_bind     complement(1307..1327)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_binding    complement(1333..1355)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(1695..2377)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     CDS             complement(2475..3134)
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      complement(3595..3901)
                     /direction=LEFT
                     /locus_tag="F1 ori"
                     /label=F1 ori
     CDS             complement(3920..3988)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattca aggcctctcg agtctagagc
      121 agaaatcggt actggctttc cattcgaccc ccattatgtg gaagtcctgg gcgagcgcat
      181 gcactacgtc gatgttggtc cacgcgatgg cacccctgtg ctgttcctgc acggtaaccc
      241 aacctcctcc tacgtgtggc gcaacatcat cccacatgtt gcaccaaccc atcgctgcat
      301 tgctccagac ctgatcggta tgggcaaatc cgacaaacca gacctgggtt atttcttcga
      361 cgaccacgtc cgcttcatgg atgccttcat cgaagccctg ggtctggaag aggtcgtcct
      421 ggtcattcac gactggggct ccgctctggg tttccactgg gccaagcgca atccagagcg
      481 cgtcaaaggt attgcattta tggagttcat ccgccctatc ccaacctggg acgaatggcc
      541 agaatttgcc cgcgagacct tccaggcctt ccgcaccacc gacgtcggcc gcaagctgat
      601 catcgatcag aacgttttta tcgagggtac actgccaatg ggtgtcgtcc gcccactgac
      661 tgaagtcgag atggaccatt accgcgagcc attcctgaat cctgttgacc gcgagccact
      721 gtggcgcttc ccaaacgagc tgccaatcgc cggtgagcca gctaacatcg tcgctctggt
      781 cgaagaatac atggactggc tgcaccagtc ccctgtccca aagctgctgt tctggggcac
      841 cccaggcgtt ctgatcccac cagccgaagc cgctcgcctg gccaaaagcc tgcctaactg
      901 caaggctgtg gacatcggcc caggtctgaa tctgctgcaa gaagacaacc cagacctgat
      961 cggcagcgag atcgctcgct ggctgagcac actcgagatt tccggctaat gtacaaggtt
     1021 acgagatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     1081 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     1141 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     1201 ggaggtgtgg gaggtttttt aattcgcggc cgcggcgcca atgcattggg cccggtaccc
     1261 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     1321 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     1381 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     1441 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     1501 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     1561 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1621 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1681 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1741 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1801 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1861 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1921 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1981 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2041 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2101 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     2161 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2221 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2281 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     2341 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     2401 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     2461 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     2521 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     2581 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     2641 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     2701 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     2761 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     2821 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     2881 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     2941 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3001 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3061 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     3121 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     3181 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     3241 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     3301 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     3361 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     3421 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
     3481 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     3541 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     3601 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     3661 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     3721 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     3781 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3841 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3901 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     3961 cggtgcgggc ctcttcgcta ttacgccagt cgaccatagc caattcaata tggcgtatat
     4021 ggactcatgc caattcaata tggtggatct ggacctgtgc caattcaata tggcgtatat
     4081 ggactcgtgc caattcaata tggtggatct ggaccccagc caattcaata tggcggactt
     4141 ggcaccatgc caattcaata tggcggactt ggcactgtgc caactgggga ggggtctact
     4201 tggcacggtg ccaagtttga ggaggggtct tggccctgtg ccaagtccgc catattgaat
     4261 tggcatggtg ccaataatgg cggccatatt ggctatatgc caggatcaat atataggcaa
     4321 tatccaatat ggccctatgc caatatggct attggccagg ttcaatacta tgtattggcc
     4381 ctatgccata tagtattcca tatatgggtt ttcctattga cgtagatagc ccctcccaat
     4441 gggcggtccc atataccata tatggggctt cctaataccg cccatagcca ctcccccatt
     4501 gacgtcaatg gtctctatat atggtctttc ctattgacgt catatgggcg gtcctattga
     4561 cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa tggcccgcct ggctcaatgc
     4621 ccattgacgt caataggacc acccaccatt gacgtcaatg ggatggctca ttgcccattc
     4681 atatccgttc tcacgccccc tattgacgtc aatgacggta aatggcccac ttggcagtac
     4741 atcaatatct attaatagta acttggcaag tacattacta ttggaaggac gccagggtac
     4801 attggcagta ctcccattga cgtcaatggc ggtaaatggc ccgcgatggc tgccaagtac
     4861 atccccattg acgtcaatgg ggaggggcaa tgacgcaaat gggcgttcca ttgacgtaaa
     4921 tgggcggtag gcgtgcctaa tgggaggtct atataagcaa tgctcgttta gggaaccctg
     4981 gtnctactca cttctccttc tctactnact aactctntct agcttgctac ctnctctaac
     5041 taccctnctt ctcacctcct nctaacagng ctnactactc ctnctcaatg tacctcaagt
//
