LOCUS       Exported               14404 bp DNA     circular SYN 09-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 14404)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Mar 24, 2022 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14404
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        complement(102..120)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        909..1013
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1014..1874
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2045..2633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2879..3208
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3059..3194
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4342..4407
                     /label=small t intron
     CDS             4537..4557
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4982..5116
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             5285..5918
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5965..6090
                     /label=HIV-1 psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6582..6815
                     /label=HIV-1 RRE
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
     misc_feature    7329..7446
                     /label=HIV-1 cPPT
                     /note="central polypurine tract from pol gene of human 
                     immunodeficiency virus type 1"
     promoter        7509..7719
                     /label=SV40 promoter
     rep_origin      7578..7713
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7735..7755
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      7804..7813
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             7810..11913
                     /codon_start=1
                     /label=dCas9
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11917..11943
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             11962..11982
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11989..12009
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     12043..12065
                     /label=BamHI-5xGCN4-F
     CDS             12073..12129
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12196..12252
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12319..12375
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12442..12498
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12565..12621
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(12666..12697)
                     /label=NotI-5xGCN4-R
     CDS             12712..12732
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     12736..12768
                     /label=NheI-P2A-F
     CDS             12742..12798
                     /codon_start=1
                     /label=P2A
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             12811..13509
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(13487..13509)
                     /label=EcoRI-TagBFP-R
     CDS             13519..13539
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    13561..14149
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
     CDS             complement(14032..14043)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    join(14248..14404,1..77)
                     /label=3'LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     LTR             join(14248..14404,1..77)
                     /label=3' LTR (Delta-U3)
     LTR             join(14301..14404,1..77)
                     /label=5' LTR
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
       61 gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat
      121 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
      181 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
      241 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
      301 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
      361 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
      421 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
      481 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
      541 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
      601 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct agctctgatg
      661 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
      721 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
      781 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
      841 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
      901 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      961 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1021 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1081 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1141 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1201 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1261 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1321 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1381 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1441 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1501 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1561 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1621 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1681 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1741 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1801 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1861 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1921 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1981 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2041 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2101 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2161 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     2221 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2281 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2341 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2401 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2461 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2521 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2581 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2641 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2701 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2761 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2821 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
     2881 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     2941 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3001 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3061 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3121 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3181 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
     3241 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     3301 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     3361 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     3421 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     3481 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
     3541 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     3601 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
     3661 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
     3721 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
     3781 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
     3841 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
     3901 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
     3961 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
     4021 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
     4081 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
     4141 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
     4201 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
     4261 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
     4321 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
     4381 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
     4441 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
     4501 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
     4561 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
     4621 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
     4681 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
     4741 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
     4801 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
     4861 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     4921 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     4981 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5041 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5101 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     5161 cccataccct attaccactg ccaattacct agtggtttca tttactctaa acctgtgatt
     5221 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
     5281 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
     5341 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     5401 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     5461 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     5521 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     5581 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     5641 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     5701 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     5761 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5821 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5881 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     5941 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     6001 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     6061 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     6121 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     6181 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     6241 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     6301 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     6361 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     6421 cagcaagcgg ccggtgatct tcagacctgg acgatatata tgagggacaa ttggagaagt
     6481 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     6541 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     6601 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     6661 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     6721 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     6781 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     6841 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     6901 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     6961 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     7021 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     7081 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     7141 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     7201 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     7261 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
     7321 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     7381 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     7441 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
     7501 gaattcaggc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     7561 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7621 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     7681 tccagaagta gtgaggaggc ttttttggag gcctaggcta cgcgcgccac catgcccaag
     7741 aagaagcgca aggtgggacg cgtctgcagg atatcaagct tgcggtaccg cgggcccggg
     7801 atcgccacca tggacaagaa gtacagcatc ggcctggcca tcggcaccaa ctctgtgggc
     7861 tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac
     7921 accgaccggc acagcatcaa gaagaacctg atcggcgccc tgctgttcga cagcggagaa
     7981 acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac
     8041 cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc
     8101 ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc
     8161 atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac
     8221 ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc
     8281 ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac
     8341 aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag
     8401 gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc
     8461 aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg
     8521 ttcggcaacc tgattgccct gagcctgggc ctgaccccca acttcaagag caacttcgac
     8581 ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga cctggacaac
     8641 ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc
     8701 gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa ggcccccctg
     8761 agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct gctgaaagct
     8821 ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca gagcaagaac
     8881 ggctacgccg gctacatcga tggcggagcc agccaggaag agttctacaa gttcatcaag
     8941 cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa cagagaggac
     9001 ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat ccacctggga
     9061 gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa ggacaaccgg
     9121 gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc tctggccagg
     9181 ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac cccctggaac
     9241 ttcgaggaag tggtggacaa gggcgccagc gcccagagct tcatcgagcg gatgaccaac
     9301 ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac
     9361 ttcaccgtgt acaacgagct gaccaaagtg aaatacgtga ccgagggaat gagaaagccc
     9421 gccttcctga gcggcgagca gaaaaaagcc atcgtggacc tgctgttcaa gaccaaccgg
     9481 aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg cttcgactcc
     9541 gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata ccacgatctg
     9601 ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga cattctggaa
     9661 gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga acggctgaaa
     9721 acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg gagatacacc
     9781 ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca gtccggcaag
     9841 acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat gcagctgatc
     9901 cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc cggccagggc
     9961 gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa gaagggcatc
    10021 ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca caagcccgag
    10081 aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca gaagaacagc
    10141 cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca gatcctgaaa
    10201 gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag
    10261 aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc cgactacgat
    10321 gtggacgcta tcgtgcctca gagctttctg aaggacgact ccatcgataa caaagtgctg
    10381 actcggagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag
    10441 aagatgaaga actactggcg ccagctgctg aatgccaagc tgattaccca gaggaagttc
    10501 gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc cggcttcatc
    10561 aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat cctggactcc
    10621 cggatgaaca ctaagtacga cgagaacgac aaactgatcc gggaagtgaa agtgatcacc
    10681 ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa agtgcgcgag
    10741 atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg
    10801 atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac
    10861 gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc caagtacttc
    10921 ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa cggcgagatc
    10981 cggaagcggc ctctgatcga gacaaacggc gaaacaggcg agatcgtgtg ggataagggc
    11041 cgggactttg ccaccgtgcg gaaagtgctg tctatgcccc aagtgaatat cgtgaaaaag
    11101 accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag gaacagcgac
    11161 aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt cgacagcccc
    11221 accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc caagaaactg
    11281 aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt cgagaagaat
    11341 cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct gatcatcaag
    11401 ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct ggcctctgcc
    11461 ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa cttcctgtac
    11521 ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca gaaacagctg
    11581 tttgtggaac agcacaaaca ctacctggac gagatcatcg agcagatcag cgagttctcc
    11641 aagagagtga tcctggccga cgctaatctg gacaaggtgc tgagcgccta caacaagcac
    11701 agagacaagc ctatcagaga gcaggccgag aatatcatcc acctgtttac cctgaccaat
    11761 ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa gaggtacacc
    11821 agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg cctgtacgag
    11881 acacggatcg acctgtctca gctgggaggc gacgcctatc cctatgacgt gcccgattat
    11941 gccagcctgg gcagcggctc ccccaagaaa aaacgcaagg tggaagatcc taagaaaaag
    12001 cggaaagtgg acggcattgg tagtgggagc aacggcagca gcggatccaa cggtccgact
    12061 gacgccgcgg aagaggagct tctgagcaaa aactatcacc tcgaaaacga ggttgcgcga
    12121 ctgaagaaag gaagcgggtc cggtggaagt ggctccggat ctggaggttc tggcagcgga
    12181 ggtagcggca gtggcgaaga gctccttagt aagaactatc atctggaaaa tgaggtagcg
    12241 cgcttaaaga aagggtcggg aagtggcggc agcggaagtg ggagtggagg gagcggttct
    12301 ggcggttccg gcagtggaga ggagttgctg tctaagaact accacttaga aaacgaagtc
    12361 gcacggctaa aaaaaggttc cggctccggc ggctccggtt ctggaagcgg gggctcggga
    12421 tcaggtggat ctggatcagg agaggaattg ctttccaaaa actaccacct tgagaatgag
    12481 gtggccaggt taaagaaggg gagcggctcg gggggtagtg gatcggggtc gggcgggtca
    12541 ggaagcggtg gtagcggatc tggggaggag ctgctctcga agaattacca tttggagaac
    12601 gaagtggcga gactaaagaa gggaagcggt agtggtggtt cagggtctgg ttcaggtggc
    12661 agtgggtctg ggggctcagg gtccgggtcg cggccgcaag gtggaggtgg acccaagaag
    12721 aagcgcaagg tgggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg
    12781 gaggagaacc ctggacctgc accaggaagt atgagcgagc tgattaagga gaacatgcac
    12841 atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac atccgagggc
    12901 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct
    12961 ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa gaccttcatc
    13021 aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg cttcacatgg
    13081 gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc
    13141 caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac atccaacggc
    13201 cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct gtaccccgct
    13261 gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg gagccatctg
    13321 atcgcaaaca tcaagaccac atatagatcc aagaaacccg ctaagaacct caagatgcct
    13381 ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa cgagacctac
    13441 gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa actggggcac
    13501 aagcttaatg gtggaggtcc aaaaaagaaa aggaaggtct aacctgcagg gcttatcgat
    13561 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
    13621 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
    13681 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
    13741 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
    13801 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
    13861 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
    13921 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
    13981 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
    14041 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
    14101 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tggtacctgc
    14161 agggatcgat cactgaggta cctttaagac caatgactta caaggcagct gtagatctta
    14221 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
    14281 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
    14341 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
    14401 tagt
//
