LOCUS       TagRFP_NES              7259 bp    DNA        circular     16-MAR-2022
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..242
                     /locus_tag="SV40 enhancer"
                     /label="SV40 enhancer"
                     /ApEinfo_label="SV40 enhancer"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    243..1561
                     /locus_tag="mEF1 promoter"
                     /label="mEF1 promoter"
                     /ApEinfo_label="mEF1 promoter"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1597..1822
                     /locus_tag="SV40 pA"
                     /label="SV40 pA"
                     /ApEinfo_label="SV40 pA"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2570..2988
                     /locus_tag="CMV enhancer"
                     /label="CMV enhancer"
                     /ApEinfo_label="CMV enhancer"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2989..4307
                     /locus_tag="rEF1 promoter"
                     /label="rEF1 promoter"
                     /ApEinfo_label="rEF1 promoter"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5106..5574
                     /locus_tag="FMDV IRES"
                     /label="FMDV IRES"
                     /ApEinfo_label="FMDV IRES"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5649..6674
                     /locus_tag="HygR"
                     /label="HygR"
                     /ApEinfo_label="HygR"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6675..7259
                     /locus_tag="EF1 pA"
                     /label="EF1 pA"
                     /ApEinfo_label="EF1 pA"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1823..2569
                     /locus_tag="pMB1 ori"
                     /label="pMB1 ori"
                     /ApEinfo_label="pMB1 ori"
                     /ApEinfo_fwdcolor="#ff8040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4308..4313
                     /locus_tag="BspEI"
                     /label="BspEI"
                     /ApEinfo_label="BspEI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5088..5093
                     /locus_tag="BamHI"
                     /label="BamHI"
                     /ApEinfo_label="BamHI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4314..5045
                     /locus_tag="TagRFP"
                     /label="TagRFP"
                     /ApEinfo_label="TagRFP"
                     /ApEinfo_fwdcolor="#fe0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5055..5084
                     /locus_tag="NES"
                     /label="NES"
                     /ApEinfo_label="NES"
                     /ApEinfo_fwdcolor="#006fbf"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 CCTGCAGGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT TAGGTACCTT CTGAGGCGGA
       61 AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA
      121 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA
      181 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC
      241 CCACTAGTGG AGCCGAGAGT AATTCATACA AAAGGAGGGA TCGCCTTCGC AAGGGGAGAG
      301 CCCAGGGACC GTCCCTAAAT TCTCACAGAC CCAAATCCCT GTAGCCGCCC CACGACAGCG
      361 CGAGGAGCAT GCGCTCAGGG CTGAGCGCGG GGAGAGCAGA GCACACAAGC TCATAGACCC
      421 TGGTCGTGGG GGGGAGGACC GGGGAGCTGG CGCGGGGCAA ACTGGGAAAG CGGTGTCGTG
      481 TGCTGGCTCC GCCCTCTTCC CGAGGGTGGG GGAGAACGGT ATATAAGTGC GGCAGTCGCC
      541 TTGGACGTTC TTTTTCGCAA CGGGTTTGCC GTCAGAACGC AGGTGAGGGG CGGGTGTGGC
      601 TTCCGCGGGC CGCCGAGCTG GAGGTCCTGC TCCGAGCGGG CCGGGCCCCG CTGTCGTCGG
      661 CGGGGATTAG CTGCGAGCAT TCCCGCTTCG AGTTGCGGGC GGCGCGGGAG GCAGAGTGCG
      721 AGGCCTAGCG GCAACCCCGT AGCCTCGCCT CGTGTCCGGC TTGAGGCCTA GCGTGGTGTC
      781 CGCGCCGCCG CCGCGTGCTA CTCCGGCCGC ACTCTGGTCT TTTTTTTTTT TGTTGTTGTT
      841 GCCCTGCTGC CTTCGATTGC CGTTCAGCAA TAGGGGCTAA CAAAGGGAGG GTGCGGGGCT
      901 TGCTCGCCCG GAGCCCGGAG AGGTCATGGT TGGGGAGGAA TGGAGGGACA GGAGTGGCGG
      961 CTGGGGCCCG CCCGCCTTCG GAGCACATGT CCGACGCCAC CTGGATGGGG CGAGGCCTGG
     1021 GGTTTTTCCC GAAGCAACCA GGCTGGGGTT AGCGTGCCGA GGCCATGTGG CCCCAGCACC
     1081 CGGCACGATC TGGCTTGGCG GCGCCGCGTT GCCCTGCCTC CCTAACTAGG GTGAGGCCAT
     1141 CCCGTCCGGC ACCAGTTGCG TGCGTGGAAA GATGGCCGCT CCCGGGCCCT GTTGCAAGGA
     1201 GCTCAAAATG GAGGACGCGG CAGCCCGGTG GAGCGGGCGG GTGAGTCACC CACACAAAGG
     1261 AAGAGGGCCT GGTCCCTCAC CGGCTGCTGC TTCCTGTGAC CCCGTGGTCC TATCGGCCGC
     1321 AATAGTCACC TCGGGCTTTT GAGCACGGCT AGTCGCGGCG GGGGGAGGGG ATGTAATGGC
     1381 GTTGGAGTTT GTTCACATTT GGTGGGTGGA GACTAGTCAG GCCAGCCTGG CGCTGGAAGT
     1441 CATTTTTGGA ATTTGTCCCC TTGAGTTTTG AGCGGAGCTA ATTCTCGGGC TTCTTAGCGG
     1501 TTCAAAGGTA TCTTTTAAAC CCTTTTTTAG GTGTTGTGAA AACCACCGCT AATTCAAAGC
     1561 AACCGGTGAT ATCAAAGATC TTGTACAGCT AGCTGGCCAG ACATGATAAG ATACATTGAT
     1621 GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT
     1681 GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT
     1741 TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA
     1801 AACCTCTACA AATGTGGTAT GGAAATGTTA ATTAACTAGC CATGACCAAA ATCCCTTAAC
     1861 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG
     1921 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG
     1981 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA
     2041 GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA
     2101 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA
     2161 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC
     2221 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA
     2281 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA
     2341 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC
     2401 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC
     2461 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG
     2521 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTA ATTAACCTGC
     2581 AGGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC
     2641 ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG
     2701 TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT
     2761 GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA
     2821 GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT
     2881 TACCATGATG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG
     2941 GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGAC TAGTGGAGCC
     3001 GAGAGTAATT CATACAAAAG GAGGGATCGC CTTCGCAAGG GGAGAGCCCA GGGACCGTCC
     3061 CTAAATTCTC ACAGACCCAA ATCCCTGTAG CCGCCCCACG ACAGCGCGAG GAGCATGCGC
     3121 CCAGGGCTGA GCGCGGGTAG ATCAGAGCAC ACAAGCTCAC AGTCCCCGGC GGTGGGGGGA
     3181 GGGGCGCGCT GAGCGGGGGC CAGGGAGCTG GCGCGGGGCA AACTGGGAAA GTGGTGTCGT
     3241 GTGCTGGCTC CGCCCTCTTC CCGAGGGTGG GGGAGAACGG TATATAAGTG CGGTAGTCGC
     3301 CTTGGACGTT CTTTTTCGCA ACGGGTTTGC CGTCAGAACG CAGgtgagtg gcgggtgtgg
     3361 cttccgcggg ccccggagct ggagccctgc tctgagcggg ccgggctgat atgcgagtgt
     3421 cgtccgcagg gtttagctgt gagcattccc acttcgagtg gcgggcggtg cgggggtgag
     3481 agtgcgaggc ctagcggcaa ccccgtagcc tcgcctcgtg tccggcttga ggcctagcgt
     3541 ggtgtccgcc gccgcgtgcc actccggccg cactatgcgt tttttgtcct tgctgccctc
     3601 gattgccttc cagcagcatg ggctaacaaa gggagggtgt ggggctcact cttaaggagc
     3661 ccatgaagct tacgttggat aggaatggaa gggcaggagg ggcgactggg gcccgcccgc
     3721 cttcggagca catgtccgac gccacctgga tggggcgagg cctgtggctt tccgaagcaa
     3781 tcgggcgtga gtttagccta cctgggccat gtggccctag cactgggcac ggtctggcct
     3841 ggcggtgccg cgttcccttg cctcccaaca agggtgaggc cgtcccgccc ggcaccagtt
     3901 gcttgcgcgg aaagatggcc gctcccgggg ccctgttgca aggagctcaa aatggaggac
     3961 gcggcagccc ggtggagcgg gcgggtgagt cacccacaca aaggaagagg gccttgcccc
     4021 tcgccggccg ctgcttcctg tgaccccgtg gtctatcggc cgcatagtca cctcgggctt
     4081 ctcttgagca ccgctcgtcg cggcgggggg aggggatcta atggcgttgg agtttgttca
     4141 catttggtgg gtggagacta gtcaggccag cctggcgctg gaagtcattc ttggaatttg
     4201 cccctttgag tttggagcga ggctaattct caagcctctt agcggttcaa aggtattttc
     4261 taaacccgtt tccagGTGTT GTGAAAGCCA CCGCTAATTC AAAGCAATCC GGAATGGTGT
     4321 CAAAAGGTGA AGAACTGATT AAGGAAAATA TGCACATGAA GTTATACATG GAAGGCACGG
     4381 TCAATAATCA CCACTTTAAA TGTACATCTG AAGGAGAGGG AAAACCGTAC GAAGGAACTC
     4441 AGACTATGCG AATCAAAGTC GTGGAGGGTG GCCCGCTGCC TTTCGCCTTC GATATTTTGG
     4501 CTACTAGCTT CATGTACGGA TCTCGCACCT TTATTAACCA TACCCAGGGA ATCCCAGATT
     4561 TCTTTAAACA ATCGTTCCCG GAAGGTTTCA CGTGGGAAAG AGTGACGACA TACGAAGATG
     4621 GAGGAGTCCT CACCGCTACC CAGGACACGA GTTTGCAAGA TGGATGCTTG ATCTATGATG
     4681 TAAAGATTCG AGGCGTAAAT TTCCCTTCGA ACGGCCCAGT CATGCAAAAG AAGACTTTGG
     4741 GATGGGAAGC AAATACTGAA ATGCTTTATC CTGCTGATGG AGGTCTTGAA GGACGTACGG
     4801 ATATGGCACT GAAACTGGTG GGCGGAGGAC ACTTGATATG TAATTTCAAA ACTACGTATC
     4861 GAAGTAAAAA GCCGGCCAAG AATCTGAAAA TGCCAGGAGT TTATTACGTT GACCACAGAC
     4921 TTGAAAGAAT TAAGGAGGCA GATAAAGAAA CGTACGTGGA ACAACACGAA GTAGCCGTTG
     4981 CTCGCTATTG TGACTTGCCA TCGAAACTTG GACATAAACT TAATGGAATG GATGAACTTT
     5041 ATAAAggaag ctccCTCCAG AAGAAACTGG AGGAACTGGA ACTGTGAGGA TCCCGTACGC
     5101 CTAGGAGCAG GTTTCCCCAA TGACACAAAA CGTGCAACTT GAAACTCCGC CTGGTCTTTC
     5161 CAGGTCTAGA GGGGTAACAC TTTGTACTGC GTTTGGCTCC ACGCTCGATC CACTGGCGAG
     5221 TGTTAGTAAC AGCACTGTTG CTTCGTAGCG GAGCATGACG GCCGTGGGAA CTCCTCCTTG
     5281 GTAACAAGGA CCCACGGGGC CAAAAGCCAC GCCCACACGG GCCCGTCATG TGTGCAACCC
     5341 CAGCACGGCG ACTTTACTGC GAAACCCACT TTAAAGTGAC ATTGAAACTG GTACCCACAC
     5401 ACTGGTGACA GGCTAAGGAT GCCCTTCAGG TACCCCGAGG TAACACGCGA CACTCGGGAT
     5461 CTGAGAAGGG GACTGGGGCT TCTATAAAAG CGCTCGGTTT AAAAAGCTTC TATGCCTGAA
     5521 TAGGTGACCG GAGGTCGGCA CCTTTCCTTT GCAATTACTG ACCCTATGAA TACACTGACT
     5581 GTTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACT CACTATAGGA
     5641 GGGCCACCAT GAAGAAACCT GAACTGACAG CAACTTCTGT TGAGAAGTTT CTCATTGAAA
     5701 AATTTGATTC TGTTTCTGAT CTCATGCAGC TGTCTGAAGG TGAAGAAAGC AGAGCCTTTT
     5761 CTTTTGATGT TGGAGGAAGA GGTTATGTTC TGAGGGTCAA TTCTTGTGCT GATGGTTTTT
     5821 ACAAAGACAG ATATGTTTAC AGACACTTTG CCTCTGCTGC TCTGCCAATT CCAGAAGTTC
     5881 TGGACATTGG AGAATTTTCT GAATCTCTCA CCTACTGCAT CAGCAGAAGA GCACAAGGAG
     5941 TCACTCTCCA GGATCTCCCT GAAACTGAGC TGCCAGCTGT TCTGCAACCT GTTGCTGAAG
     6001 CAATGGATGC CATTGCAGCA GCTGATCTGA GCCAAACCTC TGGATTTGGT CCTTTTGGTC
     6061 CCCAAGGCAT TGGTCAGTAC ACCACTTGGA GGGATTTCAT TTGTGCCATT GCTGATCCTC
     6121 ATGTCTATCA CTGGCAGACT GTGATGGATG ACACAGTTTC TGCTTCTGTT GCTCAGGCAC
     6181 TGGATGAACT CATGCTGTGG GCAGAAGATT GTCCTGAAGT CAGACACCTG GTCCATGCTG
     6241 ATTTTGGAAG CAACAATGTT CTGACAGACA ATGGCAGAAT CACTGCAGTC ATTGACTGGT
     6301 CTGAAGCCAT GTTTGGAGAT TCTCAATATG AGGTTGCCAA CATTTTTTTT TGGAGACCTT
     6361 GGCTGGCTTG CATGGAACAA CAAACAAGAT ATTTTGAAAG AAGACACCCA GAACTGGCTG
     6421 GTTCCCCCAG ACTGAGAGCC TACATGCTCA GAATTGGCCT GGACCAACTG TATCAATCTC
     6481 TGGTTGATGG AAACTTTGAT GATGCTGCTT GGGCACAAGG AAGATGTGAT GCCATTGTGA
     6541 GGTCTGGTGC TGGAACTGTT GGAAGAACTC AAATTGCAAG AAGGTCTGCT GCTGTTTGGA
     6601 CTGATGGATG TGTTGAAGTT CTGGCTGACT CTGGAAACAG GAGACCCTCC ACAAGACCCA
     6661 GAGCCAAGGA ATGAATATTA GCTAGATTAT CCCTAATACC TGCCACCCCA CTCTTAATCA
     6721 GTGGTGGAAG AACGGTCTCA GAACTGTTTG TTTCAATTGG CCATTTAAGT TTAGTAGTAA
     6781 AAGACTGGTT AATGATAACA ATGCATCGTA AAACCTTCAG AAGGAAAGGA GAATGTTTTG
     6841 TGGACCACTT TGGTTTTCTT TTTTGCGTGT GGCAGTTTTA AGTTATTAGT TTTTAAAATC
     6901 AGTACTTTTT AATGGAAACA ACTTGACCAA AAATTTGTCA CAGAATTTTG AGACCCATTA
     6961 AAAAAGTTAA ATGAGAAACC TGTGTGTTCC TTTGGTCAAC ACCGAGACAT TTAGGTGAAA
     7021 GACATCTAAT TCTGGTTTTA CGAATCTGGA AACTTCTTGA AAATGTAATT CTTGAGTTAA
     7081 CACTTCTGGG TGGAGAATAG GGTTGTTTTC CCCCCACATA ATTGGAAGGG GAAGGAATAT
     7141 CATTTAAAGC TATGGGAGGG TTTCTTTGAT TACAACACTG GAGAGAAATG CAGCATGTTG
     7201 CTGATTGCCT GTCACTAAAA CAGGCCAAAA ACTGAGTCCT TGGGTTGCAT AGAAAGCTG
//
