LOCUS       mCardinal_NLS           7301 bp    DNA        circular     16-MAR-2022
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..242
                     /locus_tag="SV40 enhancer"
                     /label="SV40 enhancer"
                     /ApEinfo_label="SV40 enhancer"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    243..1561
                     /locus_tag="mEF1 promoter"
                     /label="mEF1 promoter"
                     /ApEinfo_label="mEF1 promoter"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1597..1822
                     /locus_tag="SV40 pA"
                     /label="SV40 pA"
                     /ApEinfo_label="SV40 pA"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2570..2988
                     /locus_tag="CMV enhancer"
                     /label="CMV enhancer"
                     /ApEinfo_label="CMV enhancer"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2989..4307
                     /locus_tag="rEF1 promoter"
                     /label="rEF1 promoter"
                     /ApEinfo_label="rEF1 promoter"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5148..5616
                     /locus_tag="FMDV IRES"
                     /label="FMDV IRES"
                     /ApEinfo_label="FMDV IRES"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5691..6716
                     /locus_tag="HygR"
                     /label="HygR"
                     /ApEinfo_label="HygR"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6717..7301
                     /locus_tag="EF1 pA"
                     /label="EF1 pA"
                     /ApEinfo_label="EF1 pA"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1823..2569
                     /locus_tag="pMB1 ori"
                     /label="pMB1 ori"
                     /ApEinfo_label="pMB1 ori"
                     /ApEinfo_fwdcolor="#ff8040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4308..4313
                     /locus_tag="BspEI"
                     /label="BspEI"
                     /ApEinfo_label="BspEI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5130..5135
                     /locus_tag="BamHI"
                     /label="BamHI"
                     /ApEinfo_label="BamHI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4314..5045
                     /locus_tag="mCardinal"
                     /label="mCardinal"
                     /ApEinfo_label="mCardinal"
                     /ApEinfo_fwdcolor="#fe0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5055..5126
                     /locus_tag="SV40 NLS"
                     /label="SV40 NLS"
                     /ApEinfo_label="SV40 NLS"
                     /ApEinfo_fwdcolor="#006fbf"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 CCTGCAGGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT TAGGTACCTT CTGAGGCGGA
       61 AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA
      121 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA
      181 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC
      241 CCACTAGTGG AGCCGAGAGT AATTCATACA AAAGGAGGGA TCGCCTTCGC AAGGGGAGAG
      301 CCCAGGGACC GTCCCTAAAT TCTCACAGAC CCAAATCCCT GTAGCCGCCC CACGACAGCG
      361 CGAGGAGCAT GCGCTCAGGG CTGAGCGCGG GGAGAGCAGA GCACACAAGC TCATAGACCC
      421 TGGTCGTGGG GGGGAGGACC GGGGAGCTGG CGCGGGGCAA ACTGGGAAAG CGGTGTCGTG
      481 TGCTGGCTCC GCCCTCTTCC CGAGGGTGGG GGAGAACGGT ATATAAGTGC GGCAGTCGCC
      541 TTGGACGTTC TTTTTCGCAA CGGGTTTGCC GTCAGAACGC AGGTGAGGGG CGGGTGTGGC
      601 TTCCGCGGGC CGCCGAGCTG GAGGTCCTGC TCCGAGCGGG CCGGGCCCCG CTGTCGTCGG
      661 CGGGGATTAG CTGCGAGCAT TCCCGCTTCG AGTTGCGGGC GGCGCGGGAG GCAGAGTGCG
      721 AGGCCTAGCG GCAACCCCGT AGCCTCGCCT CGTGTCCGGC TTGAGGCCTA GCGTGGTGTC
      781 CGCGCCGCCG CCGCGTGCTA CTCCGGCCGC ACTCTGGTCT TTTTTTTTTT TGTTGTTGTT
      841 GCCCTGCTGC CTTCGATTGC CGTTCAGCAA TAGGGGCTAA CAAAGGGAGG GTGCGGGGCT
      901 TGCTCGCCCG GAGCCCGGAG AGGTCATGGT TGGGGAGGAA TGGAGGGACA GGAGTGGCGG
      961 CTGGGGCCCG CCCGCCTTCG GAGCACATGT CCGACGCCAC CTGGATGGGG CGAGGCCTGG
     1021 GGTTTTTCCC GAAGCAACCA GGCTGGGGTT AGCGTGCCGA GGCCATGTGG CCCCAGCACC
     1081 CGGCACGATC TGGCTTGGCG GCGCCGCGTT GCCCTGCCTC CCTAACTAGG GTGAGGCCAT
     1141 CCCGTCCGGC ACCAGTTGCG TGCGTGGAAA GATGGCCGCT CCCGGGCCCT GTTGCAAGGA
     1201 GCTCAAAATG GAGGACGCGG CAGCCCGGTG GAGCGGGCGG GTGAGTCACC CACACAAAGG
     1261 AAGAGGGCCT GGTCCCTCAC CGGCTGCTGC TTCCTGTGAC CCCGTGGTCC TATCGGCCGC
     1321 AATAGTCACC TCGGGCTTTT GAGCACGGCT AGTCGCGGCG GGGGGAGGGG ATGTAATGGC
     1381 GTTGGAGTTT GTTCACATTT GGTGGGTGGA GACTAGTCAG GCCAGCCTGG CGCTGGAAGT
     1441 CATTTTTGGA ATTTGTCCCC TTGAGTTTTG AGCGGAGCTA ATTCTCGGGC TTCTTAGCGG
     1501 TTCAAAGGTA TCTTTTAAAC CCTTTTTTAG GTGTTGTGAA AACCACCGCT AATTCAAAGC
     1561 AACCGGTGAT ATCAAAGATC TTGTACAGCT AGCTGGCCAG ACATGATAAG ATACATTGAT
     1621 GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT
     1681 GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT
     1741 TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA
     1801 AACCTCTACA AATGTGGTAT GGAAATGTTA ATTAACTAGC CATGACCAAA ATCCCTTAAC
     1861 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG
     1921 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG
     1981 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA
     2041 GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA
     2101 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA
     2161 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC
     2221 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA
     2281 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA
     2341 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC
     2401 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC
     2461 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG
     2521 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTA ATTAACCTGC
     2581 AGGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC
     2641 ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG
     2701 TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT
     2761 GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA
     2821 GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT
     2881 TACCATGATG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG
     2941 GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGAC TAGTGGAGCC
     3001 GAGAGTAATT CATACAAAAG GAGGGATCGC CTTCGCAAGG GGAGAGCCCA GGGACCGTCC
     3061 CTAAATTCTC ACAGACCCAA ATCCCTGTAG CCGCCCCACG ACAGCGCGAG GAGCATGCGC
     3121 CCAGGGCTGA GCGCGGGTAG ATCAGAGCAC ACAAGCTCAC AGTCCCCGGC GGTGGGGGGA
     3181 GGGGCGCGCT GAGCGGGGGC CAGGGAGCTG GCGCGGGGCA AACTGGGAAA GTGGTGTCGT
     3241 GTGCTGGCTC CGCCCTCTTC CCGAGGGTGG GGGAGAACGG TATATAAGTG CGGTAGTCGC
     3301 CTTGGACGTT CTTTTTCGCA ACGGGTTTGC CGTCAGAACG CAGgtgagtg gcgggtgtgg
     3361 cttccgcggg ccccggagct ggagccctgc tctgagcggg ccgggctgat atgcgagtgt
     3421 cgtccgcagg gtttagctgt gagcattccc acttcgagtg gcgggcggtg cgggggtgag
     3481 agtgcgaggc ctagcggcaa ccccgtagcc tcgcctcgtg tccggcttga ggcctagcgt
     3541 ggtgtccgcc gccgcgtgcc actccggccg cactatgcgt tttttgtcct tgctgccctc
     3601 gattgccttc cagcagcatg ggctaacaaa gggagggtgt ggggctcact cttaaggagc
     3661 ccatgaagct tacgttggat aggaatggaa gggcaggagg ggcgactggg gcccgcccgc
     3721 cttcggagca catgtccgac gccacctgga tggggcgagg cctgtggctt tccgaagcaa
     3781 tcgggcgtga gtttagccta cctgggccat gtggccctag cactgggcac ggtctggcct
     3841 ggcggtgccg cgttcccttg cctcccaaca agggtgaggc cgtcccgccc ggcaccagtt
     3901 gcttgcgcgg aaagatggcc gctcccgggg ccctgttgca aggagctcaa aatggaggac
     3961 gcggcagccc ggtggagcgg gcgggtgagt cacccacaca aaggaagagg gccttgcccc
     4021 tcgccggccg ctgcttcctg tgaccccgtg gtctatcggc cgcatagtca cctcgggctt
     4081 ctcttgagca ccgctcgtcg cggcgggggg aggggatcta atggcgttgg agtttgttca
     4141 catttggtgg gtggagacta gtcaggccag cctggcgctg gaagtcattc ttggaatttg
     4201 cccctttgag tttggagcga ggctaattct caagcctctt agcggttcaa aggtattttc
     4261 taaacccgtt tccagGTGTT GTGAAAGCCA CCGCTAATTC AAAGCAATCC GGAATGGTGA
     4321 GCAAGGGCGA GGAGCTGATC AAGGAGAACA TGCACATGAA GCTGTACATG GAAGGCACCG
     4381 TGAACAACCA CCACTTCAAG TGCACCACCG AAGGGGAGGG CAAGCCCTAC GAGGGCACCC
     4441 AGACCCAGAG GATTAAGGTG GTGGAGGGAG GCCCCCTGCC GTTCGCATTC GACATCCTGG
     4501 CCACCTGCTT TATGTACGGG AGCAAGACCT TCATCAACCA CACCCAGGGC ATCCCCGATT
     4561 TCTTTAAGCA GTCCTTCCCT GAGGGCTTCA CATGGGAGAG AGTCACCACA TACGAAGACG
     4621 GGGGCGTGCT TACCGTTACC CAGGACACCA GCCTCCAGGA CGGCTGCTTG ATCTACAACG
     4681 TCAAGCTCAG AGGGGTGAAC TTCCCATCCA ACGGCCCTGT GATGCAGAAG AAAACACTCG
     4741 GCTGGGAGGC CACCACCGAG ACCCTGTACC CCGCTGACGG CGGCCTGGAA GGCAGATGCG
     4801 ACATGGCCCT GAAGCTCGTG GGCGGGGGCC ACCTGCACTG CAACCTGAAG ACCACATACA
     4861 GATCCAAGAA ACCCGCTAAG AACCTCAAGA TGCCCGGCGT CTACTTTGTG GACCGCAGAC
     4921 TGGAAAGAAT CAAGGAGGCC GACAATGAGA CCTACGTCGA GCAGCACGAG GTGGCTGTGG
     4981 CCAGATACTG CGACCTCCCT AGCAAACTGG GGCACAAACT TAATGGCATG GACGAGCTGT
     5041 ACAAGggaag ctccGATCCA AAAAAGAAGA GAAAGGTAGA CCCCAAAAAG AAAAGGAAGG
     5101 TGGACCCTAA GAAAAAGCGC AAAGTGTGAG GATCCCGTAC GCCTAGGAGC AGGTTTCCCC
     5161 AATGACACAA AACGTGCAAC TTGAAACTCC GCCTGGTCTT TCCAGGTCTA GAGGGGTAAC
     5221 ACTTTGTACT GCGTTTGGCT CCACGCTCGA TCCACTGGCG AGTGTTAGTA ACAGCACTGT
     5281 TGCTTCGTAG CGGAGCATGA CGGCCGTGGG AACTCCTCCT TGGTAACAAG GACCCACGGG
     5341 GCCAAAAGCC ACGCCCACAC GGGCCCGTCA TGTGTGCAAC CCCAGCACGG CGACTTTACT
     5401 GCGAAACCCA CTTTAAAGTG ACATTGAAAC TGGTACCCAC ACACTGGTGA CAGGCTAAGG
     5461 ATGCCCTTCA GGTACCCCGA GGTAACACGC GACACTCGGG ATCTGAGAAG GGGACTGGGG
     5521 CTTCTATAAA AGCGCTCGGT TTAAAAAGCT TCTATGCCTG AATAGGTGAC CGGAGGTCGG
     5581 CACCTTTCCT TTGCAATTAC TGACCCTATG AATACACTGA CTGTTTGACA ATTAATCATC
     5641 GGCATAGTAT ATCGGCATAG TATAATACGA CTCACTATAG GAGGGCCACC ATGAAGAAAC
     5701 CTGAACTGAC AGCAACTTCT GTTGAGAAGT TTCTCATTGA AAAATTTGAT TCTGTTTCTG
     5761 ATCTCATGCA GCTGTCTGAA GGTGAAGAAA GCAGAGCCTT TTCTTTTGAT GTTGGAGGAA
     5821 GAGGTTATGT TCTGAGGGTC AATTCTTGTG CTGATGGTTT TTACAAAGAC AGATATGTTT
     5881 ACAGACACTT TGCCTCTGCT GCTCTGCCAA TTCCAGAAGT TCTGGACATT GGAGAATTTT
     5941 CTGAATCTCT CACCTACTGC ATCAGCAGAA GAGCACAAGG AGTCACTCTC CAGGATCTCC
     6001 CTGAAACTGA GCTGCCAGCT GTTCTGCAAC CTGTTGCTGA AGCAATGGAT GCCATTGCAG
     6061 CAGCTGATCT GAGCCAAACC TCTGGATTTG GTCCTTTTGG TCCCCAAGGC ATTGGTCAGT
     6121 ACACCACTTG GAGGGATTTC ATTTGTGCCA TTGCTGATCC TCATGTCTAT CACTGGCAGA
     6181 CTGTGATGGA TGACACAGTT TCTGCTTCTG TTGCTCAGGC ACTGGATGAA CTCATGCTGT
     6241 GGGCAGAAGA TTGTCCTGAA GTCAGACACC TGGTCCATGC TGATTTTGGA AGCAACAATG
     6301 TTCTGACAGA CAATGGCAGA ATCACTGCAG TCATTGACTG GTCTGAAGCC ATGTTTGGAG
     6361 ATTCTCAATA TGAGGTTGCC AACATTTTTT TTTGGAGACC TTGGCTGGCT TGCATGGAAC
     6421 AACAAACAAG ATATTTTGAA AGAAGACACC CAGAACTGGC TGGTTCCCCC AGACTGAGAG
     6481 CCTACATGCT CAGAATTGGC CTGGACCAAC TGTATCAATC TCTGGTTGAT GGAAACTTTG
     6541 ATGATGCTGC TTGGGCACAA GGAAGATGTG ATGCCATTGT GAGGTCTGGT GCTGGAACTG
     6601 TTGGAAGAAC TCAAATTGCA AGAAGGTCTG CTGCTGTTTG GACTGATGGA TGTGTTGAAG
     6661 TTCTGGCTGA CTCTGGAAAC AGGAGACCCT CCACAAGACC CAGAGCCAAG GAATGAATAT
     6721 TAGCTAGATT ATCCCTAATA CCTGCCACCC CACTCTTAAT CAGTGGTGGA AGAACGGTCT
     6781 CAGAACTGTT TGTTTCAATT GGCCATTTAA GTTTAGTAGT AAAAGACTGG TTAATGATAA
     6841 CAATGCATCG TAAAACCTTC AGAAGGAAAG GAGAATGTTT TGTGGACCAC TTTGGTTTTC
     6901 TTTTTTGCGT GTGGCAGTTT TAAGTTATTA GTTTTTAAAA TCAGTACTTT TTAATGGAAA
     6961 CAACTTGACC AAAAATTTGT CACAGAATTT TGAGACCCAT TAAAAAAGTT AAATGAGAAA
     7021 CCTGTGTGTT CCTTTGGTCA ACACCGAGAC ATTTAGGTGA AAGACATCTA ATTCTGGTTT
     7081 TACGAATCTG GAAACTTCTT GAAAATGTAA TTCTTGAGTT AACACTTCTG GGTGGAGAAT
     7141 AGGGTTGTTT TCCCCCCACA TAATTGGAAG GGGAAGGAAT ATCATTTAAA GCTATGGGAG
     7201 GGTTTCTTTG ATTACAACAC TGGAGAGAAA TGCAGCATGT TGCTGATTGC CTGTCACTAA
     7261 AACAGGCCAA AAACTGAGTC CTTGGGTTGC ATAGAAAGCT G 
//
