LOCUS       pVITRO1_hygro_mc        7310 bp    DNA        circular     15-MAR-2022
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..242
                     /locus_tag="SV40 enhancer"
                     /label="SV40 enhancer"
                     /ApEinfo_label="SV40 enhancer"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    243..1561
                     /locus_tag="mEF1 promoter"
                     /label="mEF1 promoter"
                     /ApEinfo_label="mEF1 promoter"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1597..1822
                     /locus_tag="SV40 pA"
                     /label="SV40 pA"
                     /ApEinfo_label="SV40 pA"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2570..2988
                     /locus_tag="CMV enhancer"
                     /label="CMV enhancer"
                     /ApEinfo_label="CMV enhancer"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2989..4307
                     /locus_tag="rEF1 promoter"
                     /label="rEF1 promoter"
                     /ApEinfo_label="rEF1 promoter"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5157..5625
                     /locus_tag="FMDV IRES"
                     /label="FMDV IRES"
                     /ApEinfo_label="FMDV IRES"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5700..6725
                     /locus_tag="HygR"
                     /label="HygR"
                     /ApEinfo_label="HygR"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6726..7310
                     /locus_tag="EF1 pA"
                     /label="EF1 pA"
                     /ApEinfo_label="EF1 pA"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1823..2569
                     /locus_tag="pMB1 ori"
                     /label="pMB1 ori"
                     /ApEinfo_label="pMB1 ori"
                     /ApEinfo_fwdcolor="#ff8040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4308..4313
                     /locus_tag="BspEI"
                     /label="BspEI"
                     /ApEinfo_label="BspEI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5139..5144
                     /locus_tag="BamHI"
                     /label="BamHI"
                     /ApEinfo_label="BamHI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4314..5045
                     /locus_tag="mCardinal"
                     /label="mCardinal"
                     /ApEinfo_label="mCardinal"
                     /ApEinfo_fwdcolor="#fe0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5070..5135
                     /locus_tag="CAAX"
                     /label="CAAX"
                     /ApEinfo_label="CAAX"
                     /ApEinfo_fwdcolor="#006fbf"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 CCTGCAGGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT TAGGTACCTT CTGAGGCGGA
       61 AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA
      121 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA
      181 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC
      241 CCACTAGTGG AGCCGAGAGT AATTCATACA AAAGGAGGGA TCGCCTTCGC AAGGGGAGAG
      301 CCCAGGGACC GTCCCTAAAT TCTCACAGAC CCAAATCCCT GTAGCCGCCC CACGACAGCG
      361 CGAGGAGCAT GCGCTCAGGG CTGAGCGCGG GGAGAGCAGA GCACACAAGC TCATAGACCC
      421 TGGTCGTGGG GGGGAGGACC GGGGAGCTGG CGCGGGGCAA ACTGGGAAAG CGGTGTCGTG
      481 TGCTGGCTCC GCCCTCTTCC CGAGGGTGGG GGAGAACGGT ATATAAGTGC GGCAGTCGCC
      541 TTGGACGTTC TTTTTCGCAA CGGGTTTGCC GTCAGAACGC AGGTGAGGGG CGGGTGTGGC
      601 TTCCGCGGGC CGCCGAGCTG GAGGTCCTGC TCCGAGCGGG CCGGGCCCCG CTGTCGTCGG
      661 CGGGGATTAG CTGCGAGCAT TCCCGCTTCG AGTTGCGGGC GGCGCGGGAG GCAGAGTGCG
      721 AGGCCTAGCG GCAACCCCGT AGCCTCGCCT CGTGTCCGGC TTGAGGCCTA GCGTGGTGTC
      781 CGCGCCGCCG CCGCGTGCTA CTCCGGCCGC ACTCTGGTCT TTTTTTTTTT TGTTGTTGTT
      841 GCCCTGCTGC CTTCGATTGC CGTTCAGCAA TAGGGGCTAA CAAAGGGAGG GTGCGGGGCT
      901 TGCTCGCCCG GAGCCCGGAG AGGTCATGGT TGGGGAGGAA TGGAGGGACA GGAGTGGCGG
      961 CTGGGGCCCG CCCGCCTTCG GAGCACATGT CCGACGCCAC CTGGATGGGG CGAGGCCTGG
     1021 GGTTTTTCCC GAAGCAACCA GGCTGGGGTT AGCGTGCCGA GGCCATGTGG CCCCAGCACC
     1081 CGGCACGATC TGGCTTGGCG GCGCCGCGTT GCCCTGCCTC CCTAACTAGG GTGAGGCCAT
     1141 CCCGTCCGGC ACCAGTTGCG TGCGTGGAAA GATGGCCGCT CCCGGGCCCT GTTGCAAGGA
     1201 GCTCAAAATG GAGGACGCGG CAGCCCGGTG GAGCGGGCGG GTGAGTCACC CACACAAAGG
     1261 AAGAGGGCCT GGTCCCTCAC CGGCTGCTGC TTCCTGTGAC CCCGTGGTCC TATCGGCCGC
     1321 AATAGTCACC TCGGGCTTTT GAGCACGGCT AGTCGCGGCG GGGGGAGGGG ATGTAATGGC
     1381 GTTGGAGTTT GTTCACATTT GGTGGGTGGA GACTAGTCAG GCCAGCCTGG CGCTGGAAGT
     1441 CATTTTTGGA ATTTGTCCCC TTGAGTTTTG AGCGGAGCTA ATTCTCGGGC TTCTTAGCGG
     1501 TTCAAAGGTA TCTTTTAAAC CCTTTTTTAG GTGTTGTGAA AACCACCGCT AATTCAAAGC
     1561 AACCGGTGAT ATCAAAGATC TTGTACAGCT AGCTGGCCAG ACATGATAAG ATACATTGAT
     1621 GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT
     1681 GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT
     1741 TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA
     1801 AACCTCTACA AATGTGGTAT GGAAATGTTA ATTAACTAGC CATGACCAAA ATCCCTTAAC
     1861 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG
     1921 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG
     1981 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA
     2041 GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA
     2101 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA
     2161 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC
     2221 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA
     2281 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA
     2341 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC
     2401 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC
     2461 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG
     2521 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTA ATTAACCTGC
     2581 AGGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC
     2641 ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG
     2701 TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT
     2761 GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA
     2821 GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT
     2881 TACCATGATG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG
     2941 GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGAC TAGTGGAGCC
     3001 GAGAGTAATT CATACAAAAG GAGGGATCGC CTTCGCAAGG GGAGAGCCCA GGGACCGTCC
     3061 CTAAATTCTC ACAGACCCAA ATCCCTGTAG CCGCCCCACG ACAGCGCGAG GAGCATGCGC
     3121 CCAGGGCTGA GCGCGGGTAG ATCAGAGCAC ACAAGCTCAC AGTCCCCGGC GGTGGGGGGA
     3181 GGGGCGCGCT GAGCGGGGGC CAGGGAGCTG GCGCGGGGCA AACTGGGAAA GTGGTGTCGT
     3241 GTGCTGGCTC CGCCCTCTTC CCGAGGGTGG GGGAGAACGG TATATAAGTG CGGTAGTCGC
     3301 CTTGGACGTT CTTTTTCGCA ACGGGTTTGC CGTCAGAACG CAGgtgagtg gcgggtgtgg
     3361 cttccgcggg ccccggagct ggagccctgc tctgagcggg ccgggctgat atgcgagtgt
     3421 cgtccgcagg gtttagctgt gagcattccc acttcgagtg gcgggcggtg cgggggtgag
     3481 agtgcgaggc ctagcggcaa ccccgtagcc tcgcctcgtg tccggcttga ggcctagcgt
     3541 ggtgtccgcc gccgcgtgcc actccggccg cactatgcgt tttttgtcct tgctgccctc
     3601 gattgccttc cagcagcatg ggctaacaaa gggagggtgt ggggctcact cttaaggagc
     3661 ccatgaagct tacgttggat aggaatggaa gggcaggagg ggcgactggg gcccgcccgc
     3721 cttcggagca catgtccgac gccacctgga tggggcgagg cctgtggctt tccgaagcaa
     3781 tcgggcgtga gtttagccta cctgggccat gtggccctag cactgggcac ggtctggcct
     3841 ggcggtgccg cgttcccttg cctcccaaca agggtgaggc cgtcccgccc ggcaccagtt
     3901 gcttgcgcgg aaagatggcc gctcccgggg ccctgttgca aggagctcaa aatggaggac
     3961 gcggcagccc ggtggagcgg gcgggtgagt cacccacaca aaggaagagg gccttgcccc
     4021 tcgccggccg ctgcttcctg tgaccccgtg gtctatcggc cgcatagtca cctcgggctt
     4081 ctcttgagca ccgctcgtcg cggcgggggg aggggatcta atggcgttgg agtttgttca
     4141 catttggtgg gtggagacta gtcaggccag cctggcgctg gaagtcattc ttggaatttg
     4201 cccctttgag tttggagcga ggctaattct caagcctctt agcggttcaa aggtattttc
     4261 taaacccgtt tccagGTGTT GTGAAAGCCA CCGCTAATTC AAAGCAATCC GGAATGGTGA
     4321 GCAAGGGCGA GGAGCTGATC AAGGAGAACA TGCACATGAA GCTGTACATG GAAGGCACCG
     4381 TGAACAACCA CCACTTCAAG TGCACCACCG AAGGGGAGGG CAAGCCCTAC GAGGGCACCC
     4441 AGACCCAGAG GATTAAGGTG GTGGAGGGAG GCCCCCTGCC GTTCGCATTC GACATCCTGG
     4501 CCACCTGCTT TATGTACGGG AGCAAGACCT TCATCAACCA CACCCAGGGC ATCCCCGATT
     4561 TCTTTAAGCA GTCCTTCCCT GAGGGCTTCA CATGGGAGAG AGTCACCACA TACGAAGACG
     4621 GGGGCGTGCT TACCGTTACC CAGGACACCA GCCTCCAGGA CGGCTGCTTG ATCTACAACG
     4681 TCAAGCTCAG AGGGGTGAAC TTCCCATCCA ACGGCCCTGT GATGCAGAAG AAAACACTCG
     4741 GCTGGGAGGC CACCACCGAG ACCCTGTACC CCGCTGACGG CGGCCTGGAA GGCAGATGCG
     4801 ACATGGCCCT GAAGCTCGTG GGCGGGGGCC ACCTGCACTG CAACCTGAAG ACCACATACA
     4861 GATCCAAGAA ACCCGCTAAG AACCTCAAGA TGCCCGGCGT CTACTTTGTG GACCGCAGAC
     4921 TGGAAAGAAT CAAGGAGGCC GACAATGAGA CCTACGTCGA GCAGCACGAG GTGGCTGTGG
     4981 CCAGATACTG CGACCTCCCT AGCAAACTGG GGCACAAACT TAATGGCATG GACGAGCTGT
     5041 ACAAGTCTGG CAGCGGAGGC TCTGGAGGCA AAGAAAAGAT GAGCAAAGAT GGTAAAAAGA
     5101 AGAAAAAGAA GTCAAAGACA AAGTGTGTAA TTATGTGAGG ATCCCGTACG CCTAGGAGCA
     5161 GGTTTCCCCA ATGACACAAA ACGTGCAACT TGAAACTCCG CCTGGTCTTT CCAGGTCTAG
     5221 AGGGGTAACA CTTTGTACTG CGTTTGGCTC CACGCTCGAT CCACTGGCGA GTGTTAGTAA
     5281 CAGCACTGTT GCTTCGTAGC GGAGCATGAC GGCCGTGGGA ACTCCTCCTT GGTAACAAGG
     5341 ACCCACGGGG CCAAAAGCCA CGCCCACACG GGCCCGTCAT GTGTGCAACC CCAGCACGGC
     5401 GACTTTACTG CGAAACCCAC TTTAAAGTGA CATTGAAACT GGTACCCACA CACTGGTGAC
     5461 AGGCTAAGGA TGCCCTTCAG GTACCCCGAG GTAACACGCG ACACTCGGGA TCTGAGAAGG
     5521 GGACTGGGGC TTCTATAAAA GCGCTCGGTT TAAAAAGCTT CTATGCCTGA ATAGGTGACC
     5581 GGAGGTCGGC ACCTTTCCTT TGCAATTACT GACCCTATGA ATACACTGAC TGTTTGACAA
     5641 TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC TCACTATAGG AGGGCCACCA
     5701 TGAAGAAACC TGAACTGACA GCAACTTCTG TTGAGAAGTT TCTCATTGAA AAATTTGATT
     5761 CTGTTTCTGA TCTCATGCAG CTGTCTGAAG GTGAAGAAAG CAGAGCCTTT TCTTTTGATG
     5821 TTGGAGGAAG AGGTTATGTT CTGAGGGTCA ATTCTTGTGC TGATGGTTTT TACAAAGACA
     5881 GATATGTTTA CAGACACTTT GCCTCTGCTG CTCTGCCAAT TCCAGAAGTT CTGGACATTG
     5941 GAGAATTTTC TGAATCTCTC ACCTACTGCA TCAGCAGAAG AGCACAAGGA GTCACTCTCC
     6001 AGGATCTCCC TGAAACTGAG CTGCCAGCTG TTCTGCAACC TGTTGCTGAA GCAATGGATG
     6061 CCATTGCAGC AGCTGATCTG AGCCAAACCT CTGGATTTGG TCCTTTTGGT CCCCAAGGCA
     6121 TTGGTCAGTA CACCACTTGG AGGGATTTCA TTTGTGCCAT TGCTGATCCT CATGTCTATC
     6181 ACTGGCAGAC TGTGATGGAT GACACAGTTT CTGCTTCTGT TGCTCAGGCA CTGGATGAAC
     6241 TCATGCTGTG GGCAGAAGAT TGTCCTGAAG TCAGACACCT GGTCCATGCT GATTTTGGAA
     6301 GCAACAATGT TCTGACAGAC AATGGCAGAA TCACTGCAGT CATTGACTGG TCTGAAGCCA
     6361 TGTTTGGAGA TTCTCAATAT GAGGTTGCCA ACATTTTTTT TTGGAGACCT TGGCTGGCTT
     6421 GCATGGAACA ACAAACAAGA TATTTTGAAA GAAGACACCC AGAACTGGCT GGTTCCCCCA
     6481 GACTGAGAGC CTACATGCTC AGAATTGGCC TGGACCAACT GTATCAATCT CTGGTTGATG
     6541 GAAACTTTGA TGATGCTGCT TGGGCACAAG GAAGATGTGA TGCCATTGTG AGGTCTGGTG
     6601 CTGGAACTGT TGGAAGAACT CAAATTGCAA GAAGGTCTGC TGCTGTTTGG ACTGATGGAT
     6661 GTGTTGAAGT TCTGGCTGAC TCTGGAAACA GGAGACCCTC CACAAGACCC AGAGCCAAGG
     6721 AATGAATATT AGCTAGATTA TCCCTAATAC CTGCCACCCC ACTCTTAATC AGTGGTGGAA
     6781 GAACGGTCTC AGAACTGTTT GTTTCAATTG GCCATTTAAG TTTAGTAGTA AAAGACTGGT
     6841 TAATGATAAC AATGCATCGT AAAACCTTCA GAAGGAAAGG AGAATGTTTT GTGGACCACT
     6901 TTGGTTTTCT TTTTTGCGTG TGGCAGTTTT AAGTTATTAG TTTTTAAAAT CAGTACTTTT
     6961 TAATGGAAAC AACTTGACCA AAAATTTGTC ACAGAATTTT GAGACCCATT AAAAAAGTTA
     7021 AATGAGAAAC CTGTGTGTTC CTTTGGTCAA CACCGAGACA TTTAGGTGAA AGACATCTAA
     7081 TTCTGGTTTT ACGAATCTGG AAACTTCTTG AAAATGTAAT TCTTGAGTTA ACACTTCTGG
     7141 GTGGAGAATA GGGTTGTTTT CCCCCCACAT AATTGGAAGG GGAAGGAATA TCATTTAAAG
     7201 CTATGGGAGG GTTTCTTTGA TTACAACACT GGAGAGAAAT GCAGCATGTT GCTGATTGCC
     7261 TGTCACTAAA ACAGGCCAAA AACTGAGTCC TTGGGTTGCA TAGAAAGCTG 
//
