LOCUS       iRFP702_NES             7460 bp    DNA        circular     16-MAR-2022
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..242
                     /locus_tag="SV40 enhancer"
                     /label="SV40 enhancer"
                     /ApEinfo_label="SV40 enhancer"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    243..1561
                     /locus_tag="mEF1 promoter"
                     /label="mEF1 promoter"
                     /ApEinfo_label="mEF1 promoter"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1597..1822
                     /locus_tag="SV40 pA"
                     /label="SV40 pA"
                     /ApEinfo_label="SV40 pA"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2570..2988
                     /locus_tag="CMV enhancer"
                     /label="CMV enhancer"
                     /ApEinfo_label="CMV enhancer"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2989..4307
                     /locus_tag="rEF1 promoter"
                     /label="rEF1 promoter"
                     /ApEinfo_label="rEF1 promoter"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5307..5775
                     /locus_tag="FMDV IRES"
                     /label="FMDV IRES"
                     /ApEinfo_label="FMDV IRES"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5850..6875
                     /locus_tag="HygR"
                     /label="HygR"
                     /ApEinfo_label="HygR"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6876..7460
                     /locus_tag="EF1 pA"
                     /label="EF1 pA"
                     /ApEinfo_label="EF1 pA"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1823..2569
                     /locus_tag="pMB1 ori"
                     /label="pMB1 ori"
                     /ApEinfo_label="pMB1 ori"
                     /ApEinfo_fwdcolor="#ff8040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4308..4313
                     /locus_tag="BspEI"
                     /label="BspEI"
                     /ApEinfo_label="BspEI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5289..5294
                     /locus_tag="BamHI"
                     /label="BamHI"
                     /ApEinfo_label="BamHI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4314..5246
                     /locus_tag="iRFP702"
                     /label="iRFP702"
                     /ApEinfo_label="iRFP702"
                     /ApEinfo_fwdcolor="#fe0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5256..5285
                     /locus_tag="NES"
                     /label="NES"
                     /ApEinfo_label="NES"
                     /ApEinfo_fwdcolor="#006fbf"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 CCTGCAGGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT TAGGTACCTT CTGAGGCGGA
       61 AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA
      121 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA
      181 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC
      241 CCACTAGTGG AGCCGAGAGT AATTCATACA AAAGGAGGGA TCGCCTTCGC AAGGGGAGAG
      301 CCCAGGGACC GTCCCTAAAT TCTCACAGAC CCAAATCCCT GTAGCCGCCC CACGACAGCG
      361 CGAGGAGCAT GCGCTCAGGG CTGAGCGCGG GGAGAGCAGA GCACACAAGC TCATAGACCC
      421 TGGTCGTGGG GGGGAGGACC GGGGAGCTGG CGCGGGGCAA ACTGGGAAAG CGGTGTCGTG
      481 TGCTGGCTCC GCCCTCTTCC CGAGGGTGGG GGAGAACGGT ATATAAGTGC GGCAGTCGCC
      541 TTGGACGTTC TTTTTCGCAA CGGGTTTGCC GTCAGAACGC AGGTGAGGGG CGGGTGTGGC
      601 TTCCGCGGGC CGCCGAGCTG GAGGTCCTGC TCCGAGCGGG CCGGGCCCCG CTGTCGTCGG
      661 CGGGGATTAG CTGCGAGCAT TCCCGCTTCG AGTTGCGGGC GGCGCGGGAG GCAGAGTGCG
      721 AGGCCTAGCG GCAACCCCGT AGCCTCGCCT CGTGTCCGGC TTGAGGCCTA GCGTGGTGTC
      781 CGCGCCGCCG CCGCGTGCTA CTCCGGCCGC ACTCTGGTCT TTTTTTTTTT TGTTGTTGTT
      841 GCCCTGCTGC CTTCGATTGC CGTTCAGCAA TAGGGGCTAA CAAAGGGAGG GTGCGGGGCT
      901 TGCTCGCCCG GAGCCCGGAG AGGTCATGGT TGGGGAGGAA TGGAGGGACA GGAGTGGCGG
      961 CTGGGGCCCG CCCGCCTTCG GAGCACATGT CCGACGCCAC CTGGATGGGG CGAGGCCTGG
     1021 GGTTTTTCCC GAAGCAACCA GGCTGGGGTT AGCGTGCCGA GGCCATGTGG CCCCAGCACC
     1081 CGGCACGATC TGGCTTGGCG GCGCCGCGTT GCCCTGCCTC CCTAACTAGG GTGAGGCCAT
     1141 CCCGTCCGGC ACCAGTTGCG TGCGTGGAAA GATGGCCGCT CCCGGGCCCT GTTGCAAGGA
     1201 GCTCAAAATG GAGGACGCGG CAGCCCGGTG GAGCGGGCGG GTGAGTCACC CACACAAAGG
     1261 AAGAGGGCCT GGTCCCTCAC CGGCTGCTGC TTCCTGTGAC CCCGTGGTCC TATCGGCCGC
     1321 AATAGTCACC TCGGGCTTTT GAGCACGGCT AGTCGCGGCG GGGGGAGGGG ATGTAATGGC
     1381 GTTGGAGTTT GTTCACATTT GGTGGGTGGA GACTAGTCAG GCCAGCCTGG CGCTGGAAGT
     1441 CATTTTTGGA ATTTGTCCCC TTGAGTTTTG AGCGGAGCTA ATTCTCGGGC TTCTTAGCGG
     1501 TTCAAAGGTA TCTTTTAAAC CCTTTTTTAG GTGTTGTGAA AACCACCGCT AATTCAAAGC
     1561 AACCGGTGAT ATCAAAGATC TTGTACAGCT AGCTGGCCAG ACATGATAAG ATACATTGAT
     1621 GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT
     1681 GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT
     1741 TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA
     1801 AACCTCTACA AATGTGGTAT GGAAATGTTA ATTAACTAGC CATGACCAAA ATCCCTTAAC
     1861 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG
     1921 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG
     1981 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA
     2041 GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA
     2101 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA
     2161 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC
     2221 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA
     2281 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA
     2341 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC
     2401 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC
     2461 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG
     2521 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTA ATTAACCTGC
     2581 AGGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC
     2641 ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG
     2701 TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT
     2761 GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA
     2821 GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT
     2881 TACCATGATG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG
     2941 GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGAC TAGTGGAGCC
     3001 GAGAGTAATT CATACAAAAG GAGGGATCGC CTTCGCAAGG GGAGAGCCCA GGGACCGTCC
     3061 CTAAATTCTC ACAGACCCAA ATCCCTGTAG CCGCCCCACG ACAGCGCGAG GAGCATGCGC
     3121 CCAGGGCTGA GCGCGGGTAG ATCAGAGCAC ACAAGCTCAC AGTCCCCGGC GGTGGGGGGA
     3181 GGGGCGCGCT GAGCGGGGGC CAGGGAGCTG GCGCGGGGCA AACTGGGAAA GTGGTGTCGT
     3241 GTGCTGGCTC CGCCCTCTTC CCGAGGGTGG GGGAGAACGG TATATAAGTG CGGTAGTCGC
     3301 CTTGGACGTT CTTTTTCGCA ACGGGTTTGC CGTCAGAACG CAGgtgagtg gcgggtgtgg
     3361 cttccgcggg ccccggagct ggagccctgc tctgagcggg ccgggctgat atgcgagtgt
     3421 cgtccgcagg gtttagctgt gagcattccc acttcgagtg gcgggcggtg cgggggtgag
     3481 agtgcgaggc ctagcggcaa ccccgtagcc tcgcctcgtg tccggcttga ggcctagcgt
     3541 ggtgtccgcc gccgcgtgcc actccggccg cactatgcgt tttttgtcct tgctgccctc
     3601 gattgccttc cagcagcatg ggctaacaaa gggagggtgt ggggctcact cttaaggagc
     3661 ccatgaagct tacgttggat aggaatggaa gggcaggagg ggcgactggg gcccgcccgc
     3721 cttcggagca catgtccgac gccacctgga tggggcgagg cctgtggctt tccgaagcaa
     3781 tcgggcgtga gtttagccta cctgggccat gtggccctag cactgggcac ggtctggcct
     3841 ggcggtgccg cgttcccttg cctcccaaca agggtgaggc cgtcccgccc ggcaccagtt
     3901 gcttgcgcgg aaagatggcc gctcccgggg ccctgttgca aggagctcaa aatggaggac
     3961 gcggcagccc ggtggagcgg gcgggtgagt cacccacaca aaggaagagg gccttgcccc
     4021 tcgccggccg ctgcttcctg tgaccccgtg gtctatcggc cgcatagtca cctcgggctt
     4081 ctcttgagca ccgctcgtcg cggcgggggg aggggatcta atggcgttgg agtttgttca
     4141 catttggtgg gtggagacta gtcaggccag cctggcgctg gaagtcattc ttggaatttg
     4201 cccctttgag tttggagcga ggctaattct caagcctctt agcggttcaa aggtattttc
     4261 taaacccgtt tccagGTGTT GTGAAAGCCA CCGCTAATTC AAAGCAATCC GGAATGGCGC
     4321 GTAAGGTCGA TCTCACCTCC TGCGATCGCG AGCCGATCCA CATCCCCGGC AGCATTCAGC
     4381 CGTGCGGCTG CCTGCTAGCC TGCGACGCGC AGGCGGTGCG GATCACGCGC ATTACGGAAA
     4441 ATGCCGGCGC GTTCTTTGGA CGCGAAACTC CGCGGGTCGG TGAGCTACTC GCCGATTACT
     4501 TCGGCGAGAC CGAAGCCCAT GCGCTGCGCA ACGCACTGGC GCAGTCCTCC GATCCAAAGC
     4561 GACCGGCGCT GATCTTCGGT TGGCGCGACG GCCTGACCGG CCGCACCTTC GACATCTCGC
     4621 TGCATCGCCA TGACGGTACA TCGATCATCG AGTTCGAGCC TGCGGCGGCC GAACAGGCCG
     4681 ACAATCCGCT GCGGCTGACG CGGCAGATCA TCGCGCGCAC CAAAGAACTG AAGTCGCTCG
     4741 AAGAGATGGC CGCACGGGTG CCGCGCTATC TGCAGGCGAT GCTCGGCTAT CACCGCGTGA
     4801 TGTTGTACCG CTTCGCGGAC GACGGCTCCG GCAAAGTGAT CGGCGAGGCG AAGCGCAGCG
     4861 ACCTCGAGAG CTTTCTCGGT CAGCACTTTC CGGCGTCGCT GGTCCCGCAG CAGGCGCGGC
     4921 TACTGTACTT GAAGAACGCG ATCCGCGTGG TCTCGGATTC GCGCGGCATC AGCAGCCGGA
     4981 TCGTGCCCGA GCACGACGCC TCCGGCGCCG CGCTTGATCT GTCGTTCGCG CACCTGCGCA
     5041 GCATCTCGCC TATCCATCTC GAATTTCTGC GGAACATGGG CGTCAGCGCC TCGATGTCGC
     5101 TGTCGATCAT CATTGACGGC ACGCTATGGG GATTGATCAT CTGTCATCAT TACGAGCCGC
     5161 GTGCCGTGCC GATGGCGCAG CGCGTCGCGG CCGAAATGTT CGCCGACTTC TTATCGCTGC
     5221 ACTTCACCGC CGCCCACCAC CAACGCggaa gctccCTCCA GAAGAAACTG GAGGAACTGG
     5281 AACTGTGAGG ATCCCGTACG CCTAGGAGCA GGTTTCCCCA ATGACACAAA ACGTGCAACT
     5341 TGAAACTCCG CCTGGTCTTT CCAGGTCTAG AGGGGTAACA CTTTGTACTG CGTTTGGCTC
     5401 CACGCTCGAT CCACTGGCGA GTGTTAGTAA CAGCACTGTT GCTTCGTAGC GGAGCATGAC
     5461 GGCCGTGGGA ACTCCTCCTT GGTAACAAGG ACCCACGGGG CCAAAAGCCA CGCCCACACG
     5521 GGCCCGTCAT GTGTGCAACC CCAGCACGGC GACTTTACTG CGAAACCCAC TTTAAAGTGA
     5581 CATTGAAACT GGTACCCACA CACTGGTGAC AGGCTAAGGA TGCCCTTCAG GTACCCCGAG
     5641 GTAACACGCG ACACTCGGGA TCTGAGAAGG GGACTGGGGC TTCTATAAAA GCGCTCGGTT
     5701 TAAAAAGCTT CTATGCCTGA ATAGGTGACC GGAGGTCGGC ACCTTTCCTT TGCAATTACT
     5761 GACCCTATGA ATACACTGAC TGTTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT
     5821 ATAATACGAC TCACTATAGG AGGGCCACCA TGAAGAAACC TGAACTGACA GCAACTTCTG
     5881 TTGAGAAGTT TCTCATTGAA AAATTTGATT CTGTTTCTGA TCTCATGCAG CTGTCTGAAG
     5941 GTGAAGAAAG CAGAGCCTTT TCTTTTGATG TTGGAGGAAG AGGTTATGTT CTGAGGGTCA
     6001 ATTCTTGTGC TGATGGTTTT TACAAAGACA GATATGTTTA CAGACACTTT GCCTCTGCTG
     6061 CTCTGCCAAT TCCAGAAGTT CTGGACATTG GAGAATTTTC TGAATCTCTC ACCTACTGCA
     6121 TCAGCAGAAG AGCACAAGGA GTCACTCTCC AGGATCTCCC TGAAACTGAG CTGCCAGCTG
     6181 TTCTGCAACC TGTTGCTGAA GCAATGGATG CCATTGCAGC AGCTGATCTG AGCCAAACCT
     6241 CTGGATTTGG TCCTTTTGGT CCCCAAGGCA TTGGTCAGTA CACCACTTGG AGGGATTTCA
     6301 TTTGTGCCAT TGCTGATCCT CATGTCTATC ACTGGCAGAC TGTGATGGAT GACACAGTTT
     6361 CTGCTTCTGT TGCTCAGGCA CTGGATGAAC TCATGCTGTG GGCAGAAGAT TGTCCTGAAG
     6421 TCAGACACCT GGTCCATGCT GATTTTGGAA GCAACAATGT TCTGACAGAC AATGGCAGAA
     6481 TCACTGCAGT CATTGACTGG TCTGAAGCCA TGTTTGGAGA TTCTCAATAT GAGGTTGCCA
     6541 ACATTTTTTT TTGGAGACCT TGGCTGGCTT GCATGGAACA ACAAACAAGA TATTTTGAAA
     6601 GAAGACACCC AGAACTGGCT GGTTCCCCCA GACTGAGAGC CTACATGCTC AGAATTGGCC
     6661 TGGACCAACT GTATCAATCT CTGGTTGATG GAAACTTTGA TGATGCTGCT TGGGCACAAG
     6721 GAAGATGTGA TGCCATTGTG AGGTCTGGTG CTGGAACTGT TGGAAGAACT CAAATTGCAA
     6781 GAAGGTCTGC TGCTGTTTGG ACTGATGGAT GTGTTGAAGT TCTGGCTGAC TCTGGAAACA
     6841 GGAGACCCTC CACAAGACCC AGAGCCAAGG AATGAATATT AGCTAGATTA TCCCTAATAC
     6901 CTGCCACCCC ACTCTTAATC AGTGGTGGAA GAACGGTCTC AGAACTGTTT GTTTCAATTG
     6961 GCCATTTAAG TTTAGTAGTA AAAGACTGGT TAATGATAAC AATGCATCGT AAAACCTTCA
     7021 GAAGGAAAGG AGAATGTTTT GTGGACCACT TTGGTTTTCT TTTTTGCGTG TGGCAGTTTT
     7081 AAGTTATTAG TTTTTAAAAT CAGTACTTTT TAATGGAAAC AACTTGACCA AAAATTTGTC
     7141 ACAGAATTTT GAGACCCATT AAAAAAGTTA AATGAGAAAC CTGTGTGTTC CTTTGGTCAA
     7201 CACCGAGACA TTTAGGTGAA AGACATCTAA TTCTGGTTTT ACGAATCTGG AAACTTCTTG
     7261 AAAATGTAAT TCTTGAGTTA ACACTTCTGG GTGGAGAATA GGGTTGTTTT CCCCCCACAT
     7321 AATTGGAAGG GGAAGGAATA TCATTTAAAG CTATGGGAGG GTTTCTTTGA TTACAACACT
     7381 GGAGAGAAAT GCAGCATGTT GCTGATTGCC TGTCACTAAA ACAGGCCAAA AACTGAGTCC
     7441 TTGGGTTGCA TAGAAAGCTG    
//
