LOCUS       BFP_NLS                 7286 bp    DNA        circular     16-MAR-2022
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..242
                     /locus_tag="SV40 enhancer"
                     /label="SV40 enhancer"
                     /ApEinfo_label="SV40 enhancer"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    243..1561
                     /locus_tag="mEF1 promoter"
                     /label="mEF1 promoter"
                     /ApEinfo_label="mEF1 promoter"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1597..1822
                     /locus_tag="SV40 pA"
                     /label="SV40 pA"
                     /ApEinfo_label="SV40 pA"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2570..2988
                     /locus_tag="CMV enhancer"
                     /label="CMV enhancer"
                     /ApEinfo_label="CMV enhancer"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2989..4307
                     /locus_tag="rEF1 promoter"
                     /label="rEF1 promoter"
                     /ApEinfo_label="rEF1 promoter"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5133..5601
                     /locus_tag="FMDV IRES"
                     /label="FMDV IRES"
                     /ApEinfo_label="FMDV IRES"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5676..6701
                     /locus_tag="HygR"
                     /label="HygR"
                     /ApEinfo_label="HygR"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6702..7286
                     /locus_tag="EF1 pA"
                     /label="EF1 pA"
                     /ApEinfo_label="EF1 pA"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1823..2569
                     /locus_tag="pMB1 ori"
                     /label="pMB1 ori"
                     /ApEinfo_label="pMB1 ori"
                     /ApEinfo_fwdcolor="#ff8040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4308..4313
                     /locus_tag="BspEI"
                     /label="BspEI"
                     /ApEinfo_label="BspEI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5115..5120
                     /locus_tag="BamHI"
                     /label="BamHI"
                     /ApEinfo_label="BamHI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4314..5030
                     /locus_tag="BFP"
                     /label="BFP"
                     /ApEinfo_label="BFP"
                     /ApEinfo_fwdcolor="#fe0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5040..5111
                     /locus_tag="SV40 NLS"
                     /label="SV40 NLS"
                     /ApEinfo_label="SV40 NLS"
                     /ApEinfo_fwdcolor="#006fbf"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 CCTGCAGGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT TAGGTACCTT CTGAGGCGGA
       61 AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA
      121 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA
      181 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC
      241 CCACTAGTGG AGCCGAGAGT AATTCATACA AAAGGAGGGA TCGCCTTCGC AAGGGGAGAG
      301 CCCAGGGACC GTCCCTAAAT TCTCACAGAC CCAAATCCCT GTAGCCGCCC CACGACAGCG
      361 CGAGGAGCAT GCGCTCAGGG CTGAGCGCGG GGAGAGCAGA GCACACAAGC TCATAGACCC
      421 TGGTCGTGGG GGGGAGGACC GGGGAGCTGG CGCGGGGCAA ACTGGGAAAG CGGTGTCGTG
      481 TGCTGGCTCC GCCCTCTTCC CGAGGGTGGG GGAGAACGGT ATATAAGTGC GGCAGTCGCC
      541 TTGGACGTTC TTTTTCGCAA CGGGTTTGCC GTCAGAACGC AGGTGAGGGG CGGGTGTGGC
      601 TTCCGCGGGC CGCCGAGCTG GAGGTCCTGC TCCGAGCGGG CCGGGCCCCG CTGTCGTCGG
      661 CGGGGATTAG CTGCGAGCAT TCCCGCTTCG AGTTGCGGGC GGCGCGGGAG GCAGAGTGCG
      721 AGGCCTAGCG GCAACCCCGT AGCCTCGCCT CGTGTCCGGC TTGAGGCCTA GCGTGGTGTC
      781 CGCGCCGCCG CCGCGTGCTA CTCCGGCCGC ACTCTGGTCT TTTTTTTTTT TGTTGTTGTT
      841 GCCCTGCTGC CTTCGATTGC CGTTCAGCAA TAGGGGCTAA CAAAGGGAGG GTGCGGGGCT
      901 TGCTCGCCCG GAGCCCGGAG AGGTCATGGT TGGGGAGGAA TGGAGGGACA GGAGTGGCGG
      961 CTGGGGCCCG CCCGCCTTCG GAGCACATGT CCGACGCCAC CTGGATGGGG CGAGGCCTGG
     1021 GGTTTTTCCC GAAGCAACCA GGCTGGGGTT AGCGTGCCGA GGCCATGTGG CCCCAGCACC
     1081 CGGCACGATC TGGCTTGGCG GCGCCGCGTT GCCCTGCCTC CCTAACTAGG GTGAGGCCAT
     1141 CCCGTCCGGC ACCAGTTGCG TGCGTGGAAA GATGGCCGCT CCCGGGCCCT GTTGCAAGGA
     1201 GCTCAAAATG GAGGACGCGG CAGCCCGGTG GAGCGGGCGG GTGAGTCACC CACACAAAGG
     1261 AAGAGGGCCT GGTCCCTCAC CGGCTGCTGC TTCCTGTGAC CCCGTGGTCC TATCGGCCGC
     1321 AATAGTCACC TCGGGCTTTT GAGCACGGCT AGTCGCGGCG GGGGGAGGGG ATGTAATGGC
     1381 GTTGGAGTTT GTTCACATTT GGTGGGTGGA GACTAGTCAG GCCAGCCTGG CGCTGGAAGT
     1441 CATTTTTGGA ATTTGTCCCC TTGAGTTTTG AGCGGAGCTA ATTCTCGGGC TTCTTAGCGG
     1501 TTCAAAGGTA TCTTTTAAAC CCTTTTTTAG GTGTTGTGAA AACCACCGCT AATTCAAAGC
     1561 AACCGGTGAT ATCAAAGATC TTGTACAGCT AGCTGGCCAG ACATGATAAG ATACATTGAT
     1621 GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT
     1681 GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT
     1741 TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA
     1801 AACCTCTACA AATGTGGTAT GGAAATGTTA ATTAACTAGC CATGACCAAA ATCCCTTAAC
     1861 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG
     1921 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG
     1981 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA
     2041 GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA
     2101 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA
     2161 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC
     2221 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA
     2281 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA
     2341 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC
     2401 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC
     2461 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG
     2521 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTA ATTAACCTGC
     2581 AGGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC
     2641 ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG
     2701 TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT
     2761 GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA
     2821 GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT
     2881 TACCATGATG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG
     2941 GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGAC TAGTGGAGCC
     3001 GAGAGTAATT CATACAAAAG GAGGGATCGC CTTCGCAAGG GGAGAGCCCA GGGACCGTCC
     3061 CTAAATTCTC ACAGACCCAA ATCCCTGTAG CCGCCCCACG ACAGCGCGAG GAGCATGCGC
     3121 CCAGGGCTGA GCGCGGGTAG ATCAGAGCAC ACAAGCTCAC AGTCCCCGGC GGTGGGGGGA
     3181 GGGGCGCGCT GAGCGGGGGC CAGGGAGCTG GCGCGGGGCA AACTGGGAAA GTGGTGTCGT
     3241 GTGCTGGCTC CGCCCTCTTC CCGAGGGTGG GGGAGAACGG TATATAAGTG CGGTAGTCGC
     3301 CTTGGACGTT CTTTTTCGCA ACGGGTTTGC CGTCAGAACG CAGgtgagtg gcgggtgtgg
     3361 cttccgcggg ccccggagct ggagccctgc tctgagcggg ccgggctgat atgcgagtgt
     3421 cgtccgcagg gtttagctgt gagcattccc acttcgagtg gcgggcggtg cgggggtgag
     3481 agtgcgaggc ctagcggcaa ccccgtagcc tcgcctcgtg tccggcttga ggcctagcgt
     3541 ggtgtccgcc gccgcgtgcc actccggccg cactatgcgt tttttgtcct tgctgccctc
     3601 gattgccttc cagcagcatg ggctaacaaa gggagggtgt ggggctcact cttaaggagc
     3661 ccatgaagct tacgttggat aggaatggaa gggcaggagg ggcgactggg gcccgcccgc
     3721 cttcggagca catgtccgac gccacctgga tggggcgagg cctgtggctt tccgaagcaa
     3781 tcgggcgtga gtttagccta cctgggccat gtggccctag cactgggcac ggtctggcct
     3841 ggcggtgccg cgttcccttg cctcccaaca agggtgaggc cgtcccgccc ggcaccagtt
     3901 gcttgcgcgg aaagatggcc gctcccgggg ccctgttgca aggagctcaa aatggaggac
     3961 gcggcagccc ggtggagcgg gcgggtgagt cacccacaca aaggaagagg gccttgcccc
     4021 tcgccggccg ctgcttcctg tgaccccgtg gtctatcggc cgcatagtca cctcgggctt
     4081 ctcttgagca ccgctcgtcg cggcgggggg aggggatcta atggcgttgg agtttgttca
     4141 catttggtgg gtggagacta gtcaggccag cctggcgctg gaagtcattc ttggaatttg
     4201 cccctttgag tttggagcga ggctaattct caagcctctt agcggttcaa aggtattttc
     4261 taaacccgtt tccagGTGTT GTGAAAGCCA CCGCTAATTC AAAGCAATCC GGAATGGTGA
     4321 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG
     4381 TAAACGGCCA CAAGTTCAGC GTGAGGGGCG AGGGCGAGGG CGATGCCACC AACGGCAAGC
     4441 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA
     4501 CCACCCTGAG CCACGGCGTG CAGTGCTTCG CCCGCTACCC CGACCACATG AAGCAGCACG
     4561 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG
     4621 ACGACGGCAC CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC
     4681 GCATCGAGCT GAAGGGCGTC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG
     4741 AGTACAACTT CAACAGCCAC AACATCTATA TCATGGCCGT CAAGCAGAAG AACGGCATCA
     4801 AGGTGAACTT CAAGATCCGC CACAACGTGG AGGACGGCAG CGTGCAGCTC GCCGACCACT
     4861 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAGC CACTACCTGA
     4921 GCACCCAGTC CGTGCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG
     4981 AGTTCCGCAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG ggaagctccG
     5041 ATCCAAAAAA GAAGAGAAAG GTAGACCCCA AAAAGAAAAG GAAGGTGGAC CCTAAGAAAA
     5101 AGCGCAAAGT GTGAGGATCC CGTACGCCTA GGAGCAGGTT TCCCCAATGA CACAAAACGT
     5161 GCAACTTGAA ACTCCGCCTG GTCTTTCCAG GTCTAGAGGG GTAACACTTT GTACTGCGTT
     5221 TGGCTCCACG CTCGATCCAC TGGCGAGTGT TAGTAACAGC ACTGTTGCTT CGTAGCGGAG
     5281 CATGACGGCC GTGGGAACTC CTCCTTGGTA ACAAGGACCC ACGGGGCCAA AAGCCACGCC
     5341 CACACGGGCC CGTCATGTGT GCAACCCCAG CACGGCGACT TTACTGCGAA ACCCACTTTA
     5401 AAGTGACATT GAAACTGGTA CCCACACACT GGTGACAGGC TAAGGATGCC CTTCAGGTAC
     5461 CCCGAGGTAA CACGCGACAC TCGGGATCTG AGAAGGGGAC TGGGGCTTCT ATAAAAGCGC
     5521 TCGGTTTAAA AAGCTTCTAT GCCTGAATAG GTGACCGGAG GTCGGCACCT TTCCTTTGCA
     5581 ATTACTGACC CTATGAATAC ACTGACTGTT TGACAATTAA TCATCGGCAT AGTATATCGG
     5641 CATAGTATAA TACGACTCAC TATAGGAGGG CCACCATGAA GAAACCTGAA CTGACAGCAA
     5701 CTTCTGTTGA GAAGTTTCTC ATTGAAAAAT TTGATTCTGT TTCTGATCTC ATGCAGCTGT
     5761 CTGAAGGTGA AGAAAGCAGA GCCTTTTCTT TTGATGTTGG AGGAAGAGGT TATGTTCTGA
     5821 GGGTCAATTC TTGTGCTGAT GGTTTTTACA AAGACAGATA TGTTTACAGA CACTTTGCCT
     5881 CTGCTGCTCT GCCAATTCCA GAAGTTCTGG ACATTGGAGA ATTTTCTGAA TCTCTCACCT
     5941 ACTGCATCAG CAGAAGAGCA CAAGGAGTCA CTCTCCAGGA TCTCCCTGAA ACTGAGCTGC
     6001 CAGCTGTTCT GCAACCTGTT GCTGAAGCAA TGGATGCCAT TGCAGCAGCT GATCTGAGCC
     6061 AAACCTCTGG ATTTGGTCCT TTTGGTCCCC AAGGCATTGG TCAGTACACC ACTTGGAGGG
     6121 ATTTCATTTG TGCCATTGCT GATCCTCATG TCTATCACTG GCAGACTGTG ATGGATGACA
     6181 CAGTTTCTGC TTCTGTTGCT CAGGCACTGG ATGAACTCAT GCTGTGGGCA GAAGATTGTC
     6241 CTGAAGTCAG ACACCTGGTC CATGCTGATT TTGGAAGCAA CAATGTTCTG ACAGACAATG
     6301 GCAGAATCAC TGCAGTCATT GACTGGTCTG AAGCCATGTT TGGAGATTCT CAATATGAGG
     6361 TTGCCAACAT TTTTTTTTGG AGACCTTGGC TGGCTTGCAT GGAACAACAA ACAAGATATT
     6421 TTGAAAGAAG ACACCCAGAA CTGGCTGGTT CCCCCAGACT GAGAGCCTAC ATGCTCAGAA
     6481 TTGGCCTGGA CCAACTGTAT CAATCTCTGG TTGATGGAAA CTTTGATGAT GCTGCTTGGG
     6541 CACAAGGAAG ATGTGATGCC ATTGTGAGGT CTGGTGCTGG AACTGTTGGA AGAACTCAAA
     6601 TTGCAAGAAG GTCTGCTGCT GTTTGGACTG ATGGATGTGT TGAAGTTCTG GCTGACTCTG
     6661 GAAACAGGAG ACCCTCCACA AGACCCAGAG CCAAGGAATG AATATTAGCT AGATTATCCC
     6721 TAATACCTGC CACCCCACTC TTAATCAGTG GTGGAAGAAC GGTCTCAGAA CTGTTTGTTT
     6781 CAATTGGCCA TTTAAGTTTA GTAGTAAAAG ACTGGTTAAT GATAACAATG CATCGTAAAA
     6841 CCTTCAGAAG GAAAGGAGAA TGTTTTGTGG ACCACTTTGG TTTTCTTTTT TGCGTGTGGC
     6901 AGTTTTAAGT TATTAGTTTT TAAAATCAGT ACTTTTTAAT GGAAACAACT TGACCAAAAA
     6961 TTTGTCACAG AATTTTGAGA CCCATTAAAA AAGTTAAATG AGAAACCTGT GTGTTCCTTT
     7021 GGTCAACACC GAGACATTTA GGTGAAAGAC ATCTAATTCT GGTTTTACGA ATCTGGAAAC
     7081 TTCTTGAAAA TGTAATTCTT GAGTTAACAC TTCTGGGTGG AGAATAGGGT TGTTTTCCCC
     7141 CCACATAATT GGAAGGGGAA GGAATATCAT TTAAAGCTAT GGGAGGGTTT CTTTGATTAC
     7201 AACACTGGAG AGAAATGCAG CATGTTGCTG ATTGCCTGTC ACTAAAACAG GCCAAAAACT
     7261 GAGTCCTTGG GTTGCATAGA AAGCTG   
//
