LOCUS       BFP_CAAX                7295 bp    DNA        circular     16-MAR-2022
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..242
                     /locus_tag="SV40 enhancer"
                     /label="SV40 enhancer"
                     /ApEinfo_label="SV40 enhancer"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    243..1561
                     /locus_tag="mEF1 promoter"
                     /label="mEF1 promoter"
                     /ApEinfo_label="mEF1 promoter"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1597..1822
                     /locus_tag="SV40 pA"
                     /label="SV40 pA"
                     /ApEinfo_label="SV40 pA"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2570..2988
                     /locus_tag="CMV enhancer"
                     /label="CMV enhancer"
                     /ApEinfo_label="CMV enhancer"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2989..4307
                     /locus_tag="rEF1 promoter"
                     /label="rEF1 promoter"
                     /ApEinfo_label="rEF1 promoter"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5142..5610
                     /locus_tag="FMDV IRES"
                     /label="FMDV IRES"
                     /ApEinfo_label="FMDV IRES"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5685..6710
                     /locus_tag="HygR"
                     /label="HygR"
                     /ApEinfo_label="HygR"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6711..7295
                     /locus_tag="EF1 pA"
                     /label="EF1 pA"
                     /ApEinfo_label="EF1 pA"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1823..2569
                     /locus_tag="pMB1 ori"
                     /label="pMB1 ori"
                     /ApEinfo_label="pMB1 ori"
                     /ApEinfo_fwdcolor="#ff8040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4308..4313
                     /locus_tag="BspEI"
                     /label="BspEI"
                     /ApEinfo_label="BspEI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5124..5129
                     /locus_tag="BamHI"
                     /label="BamHI"
                     /ApEinfo_label="BamHI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4314..5030
                     /locus_tag="BFP"
                     /label="BFP"
                     /ApEinfo_label="BFP"
                     /ApEinfo_fwdcolor="#fe0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5055..5120
                     /locus_tag="CAAX"
                     /label="CAAX"
                     /ApEinfo_label="CAAX"
                     /ApEinfo_fwdcolor="#006fbf"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 CCTGCAGGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT TAGGTACCTT CTGAGGCGGA
       61 AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA
      121 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA
      181 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC
      241 CCACTAGTGG AGCCGAGAGT AATTCATACA AAAGGAGGGA TCGCCTTCGC AAGGGGAGAG
      301 CCCAGGGACC GTCCCTAAAT TCTCACAGAC CCAAATCCCT GTAGCCGCCC CACGACAGCG
      361 CGAGGAGCAT GCGCTCAGGG CTGAGCGCGG GGAGAGCAGA GCACACAAGC TCATAGACCC
      421 TGGTCGTGGG GGGGAGGACC GGGGAGCTGG CGCGGGGCAA ACTGGGAAAG CGGTGTCGTG
      481 TGCTGGCTCC GCCCTCTTCC CGAGGGTGGG GGAGAACGGT ATATAAGTGC GGCAGTCGCC
      541 TTGGACGTTC TTTTTCGCAA CGGGTTTGCC GTCAGAACGC AGGTGAGGGG CGGGTGTGGC
      601 TTCCGCGGGC CGCCGAGCTG GAGGTCCTGC TCCGAGCGGG CCGGGCCCCG CTGTCGTCGG
      661 CGGGGATTAG CTGCGAGCAT TCCCGCTTCG AGTTGCGGGC GGCGCGGGAG GCAGAGTGCG
      721 AGGCCTAGCG GCAACCCCGT AGCCTCGCCT CGTGTCCGGC TTGAGGCCTA GCGTGGTGTC
      781 CGCGCCGCCG CCGCGTGCTA CTCCGGCCGC ACTCTGGTCT TTTTTTTTTT TGTTGTTGTT
      841 GCCCTGCTGC CTTCGATTGC CGTTCAGCAA TAGGGGCTAA CAAAGGGAGG GTGCGGGGCT
      901 TGCTCGCCCG GAGCCCGGAG AGGTCATGGT TGGGGAGGAA TGGAGGGACA GGAGTGGCGG
      961 CTGGGGCCCG CCCGCCTTCG GAGCACATGT CCGACGCCAC CTGGATGGGG CGAGGCCTGG
     1021 GGTTTTTCCC GAAGCAACCA GGCTGGGGTT AGCGTGCCGA GGCCATGTGG CCCCAGCACC
     1081 CGGCACGATC TGGCTTGGCG GCGCCGCGTT GCCCTGCCTC CCTAACTAGG GTGAGGCCAT
     1141 CCCGTCCGGC ACCAGTTGCG TGCGTGGAAA GATGGCCGCT CCCGGGCCCT GTTGCAAGGA
     1201 GCTCAAAATG GAGGACGCGG CAGCCCGGTG GAGCGGGCGG GTGAGTCACC CACACAAAGG
     1261 AAGAGGGCCT GGTCCCTCAC CGGCTGCTGC TTCCTGTGAC CCCGTGGTCC TATCGGCCGC
     1321 AATAGTCACC TCGGGCTTTT GAGCACGGCT AGTCGCGGCG GGGGGAGGGG ATGTAATGGC
     1381 GTTGGAGTTT GTTCACATTT GGTGGGTGGA GACTAGTCAG GCCAGCCTGG CGCTGGAAGT
     1441 CATTTTTGGA ATTTGTCCCC TTGAGTTTTG AGCGGAGCTA ATTCTCGGGC TTCTTAGCGG
     1501 TTCAAAGGTA TCTTTTAAAC CCTTTTTTAG GTGTTGTGAA AACCACCGCT AATTCAAAGC
     1561 AACCGGTGAT ATCAAAGATC TTGTACAGCT AGCTGGCCAG ACATGATAAG ATACATTGAT
     1621 GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT
     1681 GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT
     1741 TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA
     1801 AACCTCTACA AATGTGGTAT GGAAATGTTA ATTAACTAGC CATGACCAAA ATCCCTTAAC
     1861 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG
     1921 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG
     1981 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA
     2041 GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA
     2101 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA
     2161 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC
     2221 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA
     2281 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA
     2341 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC
     2401 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC
     2461 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG
     2521 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTA ATTAACCTGC
     2581 AGGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC
     2641 ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG
     2701 TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT
     2761 GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA
     2821 GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT
     2881 TACCATGATG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG
     2941 GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGAC TAGTGGAGCC
     3001 GAGAGTAATT CATACAAAAG GAGGGATCGC CTTCGCAAGG GGAGAGCCCA GGGACCGTCC
     3061 CTAAATTCTC ACAGACCCAA ATCCCTGTAG CCGCCCCACG ACAGCGCGAG GAGCATGCGC
     3121 CCAGGGCTGA GCGCGGGTAG ATCAGAGCAC ACAAGCTCAC AGTCCCCGGC GGTGGGGGGA
     3181 GGGGCGCGCT GAGCGGGGGC CAGGGAGCTG GCGCGGGGCA AACTGGGAAA GTGGTGTCGT
     3241 GTGCTGGCTC CGCCCTCTTC CCGAGGGTGG GGGAGAACGG TATATAAGTG CGGTAGTCGC
     3301 CTTGGACGTT CTTTTTCGCA ACGGGTTTGC CGTCAGAACG CAGgtgagtg gcgggtgtgg
     3361 cttccgcggg ccccggagct ggagccctgc tctgagcggg ccgggctgat atgcgagtgt
     3421 cgtccgcagg gtttagctgt gagcattccc acttcgagtg gcgggcggtg cgggggtgag
     3481 agtgcgaggc ctagcggcaa ccccgtagcc tcgcctcgtg tccggcttga ggcctagcgt
     3541 ggtgtccgcc gccgcgtgcc actccggccg cactatgcgt tttttgtcct tgctgccctc
     3601 gattgccttc cagcagcatg ggctaacaaa gggagggtgt ggggctcact cttaaggagc
     3661 ccatgaagct tacgttggat aggaatggaa gggcaggagg ggcgactggg gcccgcccgc
     3721 cttcggagca catgtccgac gccacctgga tggggcgagg cctgtggctt tccgaagcaa
     3781 tcgggcgtga gtttagccta cctgggccat gtggccctag cactgggcac ggtctggcct
     3841 ggcggtgccg cgttcccttg cctcccaaca agggtgaggc cgtcccgccc ggcaccagtt
     3901 gcttgcgcgg aaagatggcc gctcccgggg ccctgttgca aggagctcaa aatggaggac
     3961 gcggcagccc ggtggagcgg gcgggtgagt cacccacaca aaggaagagg gccttgcccc
     4021 tcgccggccg ctgcttcctg tgaccccgtg gtctatcggc cgcatagtca cctcgggctt
     4081 ctcttgagca ccgctcgtcg cggcgggggg aggggatcta atggcgttgg agtttgttca
     4141 catttggtgg gtggagacta gtcaggccag cctggcgctg gaagtcattc ttggaatttg
     4201 cccctttgag tttggagcga ggctaattct caagcctctt agcggttcaa aggtattttc
     4261 taaacccgtt tccagGTGTT GTGAAAGCCA CCGCTAATTC AAAGCAATCC GGAATGGTGA
     4321 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG
     4381 TAAACGGCCA CAAGTTCAGC GTGAGGGGCG AGGGCGAGGG CGATGCCACC AACGGCAAGC
     4441 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA
     4501 CCACCCTGAG CCACGGCGTG CAGTGCTTCG CCCGCTACCC CGACCACATG AAGCAGCACG
     4561 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG
     4621 ACGACGGCAC CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC
     4681 GCATCGAGCT GAAGGGCGTC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG
     4741 AGTACAACTT CAACAGCCAC AACATCTATA TCATGGCCGT CAAGCAGAAG AACGGCATCA
     4801 AGGTGAACTT CAAGATCCGC CACAACGTGG AGGACGGCAG CGTGCAGCTC GCCGACCACT
     4861 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAGC CACTACCTGA
     4921 GCACCCAGTC CGTGCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG
     4981 AGTTCCGCAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TCTGGCAGCG
     5041 GAGGCTCTGG AGGCAAAGAA AAGATGAGCA AAGATGGTAA AAAGAAGAAA AAGAAGTCAA
     5101 AGACAAAGTG TGTAATTATG TGAGGATCCC GTACGCCTAG GAGCAGGTTT CCCCAATGAC
     5161 ACAAAACGTG CAACTTGAAA CTCCGCCTGG TCTTTCCAGG TCTAGAGGGG TAACACTTTG
     5221 TACTGCGTTT GGCTCCACGC TCGATCCACT GGCGAGTGTT AGTAACAGCA CTGTTGCTTC
     5281 GTAGCGGAGC ATGACGGCCG TGGGAACTCC TCCTTGGTAA CAAGGACCCA CGGGGCCAAA
     5341 AGCCACGCCC ACACGGGCCC GTCATGTGTG CAACCCCAGC ACGGCGACTT TACTGCGAAA
     5401 CCCACTTTAA AGTGACATTG AAACTGGTAC CCACACACTG GTGACAGGCT AAGGATGCCC
     5461 TTCAGGTACC CCGAGGTAAC ACGCGACACT CGGGATCTGA GAAGGGGACT GGGGCTTCTA
     5521 TAAAAGCGCT CGGTTTAAAA AGCTTCTATG CCTGAATAGG TGACCGGAGG TCGGCACCTT
     5581 TCCTTTGCAA TTACTGACCC TATGAATACA CTGACTGTTT GACAATTAAT CATCGGCATA
     5641 GTATATCGGC ATAGTATAAT ACGACTCACT ATAGGAGGGC CACCATGAAG AAACCTGAAC
     5701 TGACAGCAAC TTCTGTTGAG AAGTTTCTCA TTGAAAAATT TGATTCTGTT TCTGATCTCA
     5761 TGCAGCTGTC TGAAGGTGAA GAAAGCAGAG CCTTTTCTTT TGATGTTGGA GGAAGAGGTT
     5821 ATGTTCTGAG GGTCAATTCT TGTGCTGATG GTTTTTACAA AGACAGATAT GTTTACAGAC
     5881 ACTTTGCCTC TGCTGCTCTG CCAATTCCAG AAGTTCTGGA CATTGGAGAA TTTTCTGAAT
     5941 CTCTCACCTA CTGCATCAGC AGAAGAGCAC AAGGAGTCAC TCTCCAGGAT CTCCCTGAAA
     6001 CTGAGCTGCC AGCTGTTCTG CAACCTGTTG CTGAAGCAAT GGATGCCATT GCAGCAGCTG
     6061 ATCTGAGCCA AACCTCTGGA TTTGGTCCTT TTGGTCCCCA AGGCATTGGT CAGTACACCA
     6121 CTTGGAGGGA TTTCATTTGT GCCATTGCTG ATCCTCATGT CTATCACTGG CAGACTGTGA
     6181 TGGATGACAC AGTTTCTGCT TCTGTTGCTC AGGCACTGGA TGAACTCATG CTGTGGGCAG
     6241 AAGATTGTCC TGAAGTCAGA CACCTGGTCC ATGCTGATTT TGGAAGCAAC AATGTTCTGA
     6301 CAGACAATGG CAGAATCACT GCAGTCATTG ACTGGTCTGA AGCCATGTTT GGAGATTCTC
     6361 AATATGAGGT TGCCAACATT TTTTTTTGGA GACCTTGGCT GGCTTGCATG GAACAACAAA
     6421 CAAGATATTT TGAAAGAAGA CACCCAGAAC TGGCTGGTTC CCCCAGACTG AGAGCCTACA
     6481 TGCTCAGAAT TGGCCTGGAC CAACTGTATC AATCTCTGGT TGATGGAAAC TTTGATGATG
     6541 CTGCTTGGGC ACAAGGAAGA TGTGATGCCA TTGTGAGGTC TGGTGCTGGA ACTGTTGGAA
     6601 GAACTCAAAT TGCAAGAAGG TCTGCTGCTG TTTGGACTGA TGGATGTGTT GAAGTTCTGG
     6661 CTGACTCTGG AAACAGGAGA CCCTCCACAA GACCCAGAGC CAAGGAATGA ATATTAGCTA
     6721 GATTATCCCT AATACCTGCC ACCCCACTCT TAATCAGTGG TGGAAGAACG GTCTCAGAAC
     6781 TGTTTGTTTC AATTGGCCAT TTAAGTTTAG TAGTAAAAGA CTGGTTAATG ATAACAATGC
     6841 ATCGTAAAAC CTTCAGAAGG AAAGGAGAAT GTTTTGTGGA CCACTTTGGT TTTCTTTTTT
     6901 GCGTGTGGCA GTTTTAAGTT ATTAGTTTTT AAAATCAGTA CTTTTTAATG GAAACAACTT
     6961 GACCAAAAAT TTGTCACAGA ATTTTGAGAC CCATTAAAAA AGTTAAATGA GAAACCTGTG
     7021 TGTTCCTTTG GTCAACACCG AGACATTTAG GTGAAAGACA TCTAATTCTG GTTTTACGAA
     7081 TCTGGAAACT TCTTGAAAAT GTAATTCTTG AGTTAACACT TCTGGGTGGA GAATAGGGTT
     7141 GTTTTCCCCC CACATAATTG GAAGGGGAAG GAATATCATT TAAAGCTATG GGAGGGTTTC
     7201 TTTGATTACA ACACTGGAGA GAAATGCAGC ATGTTGCTGA TTGCCTGTCA CTAAAACAGG
     7261 CCAAAAACTG AGTCCTTGGG TTGCATAGAA AGCTG  
//
