LOCUS       pRsetB-KanR-LiLac       3877 bp ds-DNA     circular SYN 28-MAR-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Yellen Lab, HMS Neurobiology
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 28, 2022 from SnapGene 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        845..863
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             895..917
                     /note="efficient ribosome binding site from 
                     bacteriophageT7gene 10 (Olins and Rangwala, 1989)"
     CDS             925..2478
                     /codon_start=1
                     /label=LiLac
                     /translation="MKHHHHHHHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGD
                     ATYGKLTLKFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQE
                     RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNSSGRTGIDPF
                     TESVLQSQATELLQKKAQLVSFKIQGIMKRIFMGANTLEKFLSDENSAINDTLKRRMLS
                     EFLLANPHVLLVSAIYTNNNERVITAMSMDSKIAYPNTTLNENMTNQIRSLKSITHSDP
                     YYKEVNGDKIYGMDITLPLMGKNQNAIGALNFFLNIDAFYTDVVGKKKSNTFLMGKDGR
                     LLINPNREIQDKILSAINPDRRVAKAVEYYNQNEAGTLSYHSLSGNTETFLAIQPFDFF
                     EEKGNNGNHWRWAIGKYVNKSLVFSSHSNVYITADKQKNGIKANFKIRHNIEDGGVQLA
                     DHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYQ
                     "
     CDS             931..951
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     terminator      2487..2534
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2808..2912
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2913..3707
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaggatct cgatcccgcg
      841 aaattaatac gactcactat agggagacca caacggtttc cctctagaaa taattttgtt
      901 taactttaag aaggagatat acatatgaaa catcaccatc atcatcatca tgtgagcaag
      961 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1021 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1081 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1141 ctgtcctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     1201 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1261 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1321 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1381 aactcctccg gccgcaccgg cattgacccc tttactgaaa gcgtgttgca aagccaagcc
     1441 acagaattgc tgcaaaaaaa agctcagtta gtcagtttta aaattcaagg cattatgaag
     1501 cgcattttta tgggcgctaa cacccttgaa aaatttttaa gcgatgaaaa tagcgctatc
     1561 aacgacaccc tcaaacgccg catgctctct gagtttttgt tagcaaaccc tcatgtgtta
     1621 ttggttagcg cgatttatac gaataataat gaacgtgtca tcactgccat gagcatggat
     1681 tcaaaaatcg cctaccctaa taccacgctc aatgaaaaca tgaccaatca aatccgttcg
     1741 ctcaaaagta ttacccattc agatccctat tataaagagg ttaatggcga taaaatctat
     1801 ggcatggata ttaccctccc ccttatgggt aagaatcaaa atgctattgg cgcgctgaat
     1861 ttctttttaa acattgacgc tttttatacc gatgtggtag gcaagaaaaa gagcaacacc
     1921 tttttaatgg ggaaagacgg ccgtctttta atcaacccta atcgtgagat ccaagataag
     1981 attttaagcg ctatcaatcc agatcgccgt gtcgctaaag ctgtggagta ttacaatcaa
     2041 aacgaagcgg gcactttgag ctaccattca ttgagcggga atacagaaac ctttttagcc
     2101 attcagccct ttgatttttt tgaagaaaaa gggaataacg gcaatcattg gcgttgggca
     2161 attgggaaat atgtcaataa atctttagtc ttttccagcc acagcaacgt ctatatcacc
     2221 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac
     2281 ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2341 ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag
     2401 aagcgcgatc acatggtcct actggagttc gtgaccgccg ccgggatcac tctcggcatg
     2461 gacgagctgt accagtgatt tccggactag cataacccct tggggcctct aaacgggtct
     2521 tgaggggttt tttgctgagc tccgaggggc ccaagctgta gtgggccatc gccctgatag
     2581 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     2641 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     2701 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     2761 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     2821 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     2881 aaatgcttca ataatattga aaaaggaaga gtatgattga acaagatgga ttgcacgcag
     2941 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     3001 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     3061 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     3121 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     3181 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     3241 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     3301 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     3361 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     3421 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     3481 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     3541 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     3601 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     3661 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgactg tcagaccaag
     3721 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3781 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3841 gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
