LOCUS       pAAV-CAG-LiLac          6264 bp ds-DNA     circular SYN 28-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Yellen Lab, HMS Neurobiology
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 28, 2022 from SnapGene 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    18..205
                     /label=ITR
     misc_feature    209..1145
                     /label=CAG (CBA)
     CDS             1155..2711
                     /codon_start=1
                     /label=LiLac
                     /translation="MKHHHHHHHGVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEG
                     DATYGKLTLKFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQ
                     ERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNSSGRTGIDP
                     FTESVLQSQATELLQKKAQLVSFKIQGIMKRIFMGANTLEKFLSDENSAINDTLKRRML
                     SEFLLANPHVLLVSAIYTNNNERVITAMSMDSKIAYPNTTLNENMTNQIRSLKSITHSD
                     PYYKEVNGDKIYGMDITLPLMGKNQNAIGALNFFLNIDAFYTDVVGKKKSNTFLMGKDG
                     RLLINPNREIQDKILSAINPDRRVAKAVEYYNQNEAGTLSYHSLSGNTETFLAIQPFDF
                     FEEKGNNGNHWRWAIGKYVNKSLVFSSHSNVYITADKQKNGIKANFKIRHNIEDGGVQL
                     ADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELY
                     Q"
     misc_feature    2728..3315
                     /label=WPRE
     misc_feature    3354..3473
                     /label=SV40_PA_terminator
     misc_feature    3581..3770
                     /label=ITR
     misc_feature    3991..4610
                     /label=pBR322_origin
     misc_feature    complement(4765..5625)
                     /label=Ampicilin
     misc_feature    complement(5667..5695)
                     /label=Ampr promoter
BASE COUNT         1475 a         1669 c         1619 g         1500 t
ORIGIN
        1 tcttcgctat tacgccagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
       61 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      121 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      181 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
      241 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      301 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      361 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      421 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
      481 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
      541 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
      601 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
      661 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
      721 aagcgcgcgg cgggcgggag tcgctgcgac gctgccttcg ccccgtgccc cgctccgccg
      781 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
      841 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt
      901 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg
      961 gctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
     1021 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc
     1081 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
     1141 gaattggatc caccatgaaa catcaccatc accatcacca tggagtgagc aagggcgagg
     1201 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1261 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1321 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgtcct
     1381 ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1441 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1501 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1561 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactcct
     1621 ccggccgcac cggcattgac ccctttactg aaagcgtgtt gcaaagccaa gccacagaat
     1681 tgctgcaaaa aaaagctcag ttagtcagtt ttaaaattca aggcattatg aagcgcattt
     1741 ttatgggcgc taacaccctt gaaaaatttt taagcgatga aaatagcgct atcaacgaca
     1801 ccctcaaacg ccgcatgctc tctgagtttt tgttagcaaa ccctcatgtg ttattggtta
     1861 gcgcgattta tacgaataat aatgaacgtg tcatcactgc catgagcatg gattcaaaaa
     1921 tcgcctaccc taataccacg ctcaatgaaa acatgaccaa tcaaatccgt tcgctcaaaa
     1981 gtattaccca ttcagatccc tattataaag aggttaatgg cgataaaatc tatggcatgg
     2041 atattaccct cccccttatg ggtaagaatc aaaatgctat tggcgcgctg aatttctttt
     2101 taaacattga cgctttttat accgatgtgg taggcaagaa aaagagcaac acctttttaa
     2161 tggggaaaga cggccgtctt ttaatcaacc ctaatcgtga gatccaagat aagattttaa
     2221 gcgctatcaa tccagatcgc cgtgtcgcta aagctgtgga gtattacaat caaaacgaag
     2281 cgggcacttt gagctaccat tcattgagcg ggaatacaga aaccttttta gccattcagc
     2341 cctttgattt ttttgaagaa aaagggaata acggcaatca ttggcgttgg gcaattggga
     2401 aatatgtcaa taaatcttta gtcttttcca gccacagcaa cgtctatatc accgccgaca
     2461 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
     2521 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     2581 ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg
     2641 atcacatggt cctactggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     2701 tgtaccagtg atttccggaa tcgataatca acctctggat tacaaaattt gtgaaagatt
     2761 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     2821 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     2881 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     2941 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     3001 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     3061 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     3121 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     3181 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     3241 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     3301 ggccgcctcc ccgcatcgat accgtcgacc cgggcggccg cttcgagcag acatgataag
     3361 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     3421 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     3481 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta
     3541 aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatcttccta gagcatggct
     3601 acgtagataa gtagcatggc gggttaatca ttaactacaa ggaaccccta gtgatggagt
     3661 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
     3721 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcattaatg
     3781 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     3841 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3901 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3961 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4021 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4081 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4141 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4201 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4261 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4321 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4381 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4441 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4501 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4561 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4621 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4681 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4741 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4801 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4861 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4921 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4981 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5041 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     5101 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     5161 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5221 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5281 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5341 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5401 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5461 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5521 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     5581 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     5641 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5701 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt
     5761 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     5821 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     5881 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     5941 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     6001 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     6061 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     6121 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     6181 gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt
     6241 tgggaagggc gatcggtgcg ggcc
//
