LOCUS       Exported                9051 bp ds-DNA     circular SYN 27-JAN-2021
DEFINITION  synthetic circular DNA
ACCESSION   VB900088-2239fsw
VERSION     .
KEYWORDS    1531.pLV-CMV-EGFP-Hygro.dna
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 1, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9051
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /label=RSV promoter
                     /note="/application_notes=Strong promoter; drives 
                     transcription of viral RNA in packaging cells."
                     /note="/description=Rous sarcoma virus enhancer/promoter"
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
     LTR             230..410
                     /label=-Delta-5' LTR
                     /note="/application_notes=Allows transcription of viral RNA
                     and its packaging into virus."
                     /note="/description=Truncated HIV-1 5' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
     misc_feature    521..565
                     /label=-Psi
                     /label=nonstandard type: Miscellaneous
                     /note="/application_notes=Allows packaging of viral RNA 
                     into virus."
                     /note="/description=HIV-1 packaging signal"
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
     misc_feature    1075..1308
                     /label=RRE
                     /label=nonstandard type: Miscellaneous
                     /note="/application_notes=Rev protein binding site that 
                     allows Rev-dependent nuclear export of viral RNA during 
                     viral packaging."
                     /note="/description=HIV-1 Rev response element"
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
     misc_feature    1803..1920
                     /label=cPPT
                     /label=nonstandard type: Miscellaneous
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1 cDNA through a central DNA flap."
                     /note="/description=Central polypurine tract"
                     /note="/full_name=Central polypurine tract"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
     promoter        1959..2547
                     /label=CMV
                     /note="/application_notes=Strong promoter; may have 
                     variable strength in some cell types."
                     /note="/description=Human cytomegalovirus immediate early 
                     enhancer/promoter"
                     /note="/full_name=Human cytomegalovirus immediate early 
                     promoter"
                     /note="/official_designation=CMV"
                     /note="/uuid=bbb1016d51c4484eaa5a960df4edf21a"
     misc_feature    2572..2577
                     /label=Kozak
                     /label=nonstandard type: Miscellaneous
                     /note="/application_notes=Facilitates translation 
                     initiation of ATG start codon downstream of the Kozak 
                     sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     misc_feature    2578..3297
                     /label=EGFP
                     /label=nonstandard type: ORF
                     /note="/application_notes=Commonly used green fluorescent 
                     protein; ranked high in brightness, photostability and pH 
                     stability among all fluorescent proteins."
                     /note="/description=Enhanced green fluorescent protein; 
                     codon optimized based on a variant of wild type GFP from 
                     the jellyfish Aequorea victoria"
                     /note="/full_name=EGFP reporter gene"
                     /note="/official_designation=EGFP"
                     /note="/uuid=0470849bb38e4f4b8a1fe5d436422af3"
     misc_feature    3336..3933
                     /label=WPRE
                     /label=nonstandard type: Miscellaneous
                     /note="/application_notes=Enhances virus stability in 
                     packaging cells, leading to higher titer of packaged virus;
                     enhances higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis virus 
                     posttranscriptional regulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptional regulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     promoter        3952..4462
                     /label=mPGK promoter
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Mouse phosphoglycerate kinase 1 
                     promoter"
                     /note="/full_name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/official_designation=mPGK promoter"
                     /note="/uuid=bce93795b5fd4173b8b9abc832edad80"
     misc_feature    4475..5500
                     /label=Hygro
                     /label=nonstandard type: Marker
                     /note="/application_notes=Allows cells to be resistant to 
                     hygromycin B."
                     /note="/description=Hygromycin resistance gene"
                     /note="/full_name=Hygromycin resistance gene"
                     /note="/official_designation=Hygro"
                     /note="/uuid=9135dd0aa64645f3aa3c381325794022"
     LTR             5571..5805
                     /label=-Delta-U3/3' LTR
                     /note="/application_notes=Allows packaging of viral RNA 
                     into virus; self-inactivates the 5' LTR by a copying 
                     mechanism during viral genome integration; contains 
                     polyadenylation signal for transcription termination."
                     /note="/description=Truncated HIV-1 3' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
     polyA_signal    5878..6012
                     /label=SV40 early pA
                     /note="/application_notes=Allows transcription termination 
                     and polyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Simian virus 40 early polyadenylation 
                     signal"
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
     misc_feature    6966..7826
                     /label=Ampicillin
                     /label=nonstandard type: ORF
                     /note="/application_notes=Allows E. coli to be resistant to
                     ampicillin."
                     /note="/description=Ampicillin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
     rep_origin      7997..8585
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/application_notes=Facilitates plasmid replication 
                     in E. coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgta gttattaata gtaatcaatt
     1981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2281 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2341 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2401 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2461 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2521 agagctggtt tagtgaaccg tcagatccaa gtttgtacaa aaaagcaggc tgccaccatg
     2581 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     2641 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     2701 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     2761 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     2821 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     2881 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2941 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     3001 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     3061 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     3121 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     3181 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     3241 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaacc
     3301 cagctttctt gtacaaagtg gtgataatcg aattccgata atcaacctct ggattacaaa
     3361 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     3421 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     3481 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     3541 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     3601 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     3661 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3721 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     3781 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     3841 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     3901 cggatctccc tttgggccgc ctccccgcat cgggaattcc cgcggttcga attctaccgg
     3961 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     4021 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     4081 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     4141 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     4201 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     4261 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     4321 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     4381 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     4441 tcatctccgg gcctttcgac ctcacgtggc caccatgaaa aagcctgaac tcaccgcgac
     4501 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     4561 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     4621 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     4681 ggccgcgctc ccgattccgg aagtgcttga cattggggaa tttagcgaga gcctgaccta
     4741 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     4801 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     4861 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     4921 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     4981 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     5041 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     5101 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     5161 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     5221 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     5281 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     5341 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     5401 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     5461 aaaccgacgc cccagcactc gtccgagggc aaaggaatag ggtaccttta agaccaatga
     5521 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     5581 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     5641 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     5701 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     5761 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     5821 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     5881 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5941 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6001 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6061 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6121 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     6181 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     6241 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     6301 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     6361 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6421 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6481 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6541 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6601 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6661 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6721 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6781 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     6841 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6901 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6961 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     7021 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     7081 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     7141 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     7201 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7261 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7321 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7381 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7441 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7501 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7561 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     7621 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7681 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7741 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7801 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     7861 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7921 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7981 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8041 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8101 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8161 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8221 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8281 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8341 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8401 ccacgcttcc cgaagagaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8461 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8521 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8581 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8641 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     8701 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8761 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     8821 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     8881 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     8941 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     9001 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct t
//
