LOCUS       Exported                6620 bp ds-DNA     circular UNA 04-APR-2022
DEFINITION  Ligation of 2 sequences.
ACCESSION   urn.local...16-ena11kr
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 5, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6620
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..6205
                     /standard_name="pFS_1061_pET30_pCat-tetR-Term-ptetA-FsRT-C 
                     as1(opt)-Cas2(opt)-3xTerm_GGC(BbsI)-Leader-ARRAY2-DR2-FaqI 
                     _Term_NotI_ (digested by BbsI) Fragment 2 extraction 
                     (concatenated sequence 1)"
                     /label=Geneious type: Concatenated sequence
                     /note="Geneious type: Concatenated sequence"
     promoter        180..208
     CDS             complement(211..1026)
                     /standard_name="KanR"
                     /label=KanR
     misc_feature    1132..1751
                     /standard_name="pBR322 origin"
                     /label=pBR322 origin
                     /note="Geneious type: origin of replication"
     gene            complement(2166..2357)
                     /standard_name="ROP"
                     /label=ROP
     protein_bind    2841..2878
                     /standard_name="operator: P(Cat)"
                     /label=operator: P(Cat) binding site
     -35_signal      2844..2849
     -10_signal      2867..2872
     RBS             2887..2898
     gene            2905..3528
                     /standard_name="tetR gene"
                     /label=tetR gene
                     /note="tetR"
     terminator      3576..3619
     terminator      3660..3679
     promoter        3702..3759
     protein_bind    3702..3721
                     /standard_name="tetR binding site protein bind"
                     /label=tetR binding site protein bind binding site
                     /note="tetR binding site"
     -35_signal      3721..3726
     protein_bind    3727..3746
                     /standard_name="tetR binding site protein bind"
                     /label=tetR binding site protein bind binding site
                     /note="tetR binding site"
     -10_signal      3744..3749
     RBS             3770..3777
     CDS             3784..5682
                     /standard_name="FsRT-Cas1(opt.)"
                     /label=FsRT-Cas1(opt.)
                     /note="Geneious type: cds"
     gene            5689..5705
                     /standard_name="T7_transl_en_RBS"
                     /label=T7_transl_en_RBS
     CDS             5714..5974
                     /standard_name="FsCas2(opt.)"
                     /label=FsCas2(opt.)
                     /note="Geneious type: cds"
     misc_feature    join(5976..6205,6248..6620,1..5979)
                     /standard_name="FS_3046_gBlock_transcription_stimulated_sp 
                     acer_acquisition (digested by NotI, XhoI) Fragment 3 
                     (concatenated sequence 1)"
                     /label=Geneious type: Concatenated sequence
                     /note="Geneious type: Concatenated sequence"
     terminator      5992..6034
     terminator      6035..6081
     terminator      6079..6201
     terminator      6135..6189
     misc_feature    6202..6251
                     /standard_name="FS_3212.3213_J23103 (digested by BbsI)
                     Fragment 2 (concatenated sequence 2)"
                     /label=Geneious type: Concatenated sequence
                     /note="Geneious type: Concatenated sequence"
     promoter        6207..6241
     promoter        6245
     misc_feature    6248..6620
                     /standard_name="pFS_1061_pET30_pCat-tetR-Term-ptetA-FsRT-C 
                     as1(opt)-Cas2(opt)-3xTerm_GGC(BbsI)-Leader-ARRAY2-DR2-FaqI 
                     _Term_NotI_ (digested by BbsI) Fragment 2 - 
                     FS_3046_gBlock_transcription_stimulated_spacer_acquisition 
                     (digested by NotI, XhoI) Fragment 3 (concatenated sequence 
                     1) (concatenated sequence 3)"
                     /label=Geneious type: Concatenated sequence
                     /note="Geneious type: Concatenated sequence"
     promoter        6253..6443
     misc_feature    6444..6472
                     /standard_name="FsCRISPR_DR_2"
                     /label=FsCRISPR_DR_2
                     /note="Geneious type: CRISPR repeat"
     misc_feature    6478..6528
                     /standard_name="50 bp randomized"
                     /label=50 bp randomized
     terminator      complement(6529..6618)
ORIGIN
        1 ggccgctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
       61 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttca
      121 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      181 tcaaatatgt atccgctcat gaattaattc ttagaaaaac tcatcgagca tcaaatgaaa
      241 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
      301 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
      361 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
      421 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
      481 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
      541 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
      601 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
      661 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
      721 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
      781 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
      841 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
      901 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
      961 atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg
     1021 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgacca
     1081 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1141 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1201 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1261 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1321 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1381 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1441 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1501 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1561 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1621 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1681 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1741 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1801 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1861 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     1921 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg
     1981 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
     2041 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
     2101 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     2161 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca
     2221 tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg
     2281 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
     2341 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg
     2401 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
     2461 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
     2521 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc
     2581 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt
     2641 tccagacttt acgaaacacg gaaaccgaag atcattcatg ttgttgctca ggtcgcagac
     2701 gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca
     2761 gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc
     2821 cgtggggccg ccatgccggc ggcacgtaag aggttccaac tttcaccata atgaaacata
     2881 ctagagaaag aggagaaata ctagatgtcc agattagata aaagtaaagt gattaacagc
     2941 gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag
     3001 aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc
     3061 gacgccttag ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg
     3121 gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt
     3181 catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact
     3241 ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat
     3301 gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa
     3361 gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa
     3421 gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg
     3481 atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtcctaata acactgatag
     3541 tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     3601 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     3661 gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca gtgatagaga
     3721 ttgacatccc tatcagtgat agagatactg agcactacta gagaagcttg cggaggagca
     3781 tgcatgttta ccatcgacga gatgctgagc aagaacaacc aacgtctggc gttcgaacac
     3841 tttgcgacca aaaacgacgg ttgcggcccg gacggtatgc acgttagcga actggagaag
     3901 tactggcgta tgaatcacga ccagatcatt agcgatctga agaaccagga ataccagccg
     3961 ggtattatcc tgattcgtga gcacatgaat aaaaccggca aacgccgtaa tatcgcgagc
     4021 ctgaacgtta ttgatcgttt cattacccgt ctgctgagcc agaaactgaa ccgttatctg
     4081 gcgccgattt tctgcgagaa cagctatgcg taccaggaca gcaaaggcgt tatgccggcg
     4141 gttctgaaag cgaaggagta cgttgagctg ggtatgcgtc acgtgattga aatcgatctg
     4201 aaaaactact ttgacaccat tccgctggaa aacctgatcc cggagatcga acgctacatc
     4261 accgatgagg cggttctgca tctgatcaag caatacctgt tttgcgatat tagcttcgag
     4321 ggcaaaatca gccgtaaaac ccaaggtatc gttcaaggta acgcgatcag cccgatcctg
     4381 agcaacctgt acctgaacga cttcgacaag gaactggacg agagcaagct gtgctggatt
     4441 cgttacgcgg acaatatcta catctatatg gatagctatg agaaggcgct gctggtgtat
     4501 agcgagctga ccgagcgtct ggagcgtcgc aagctgaccg ttaacaagga gaaaagcggc
     4561 gtttttgatg tgagcacccg tagcattctg ggctacgaca tcctgattcg taacaagaaa
     4621 gtggacgttc gtaagcatat ttacaaaagc gttaatcagt acagcaactg gcacgatagc
     4681 cgcctggagt ttatcaacgg tcgttaccac atcaccagcg atggcatcct gaatcgccaa
     4741 gacttcggcc tgctgttcga gaatgagcag aaaaaacact acatcccggt tgaggttagc
     4801 gatcagctga acatctacgg taatgttacc ctggcgagca atgtgctgca aagtttcagc
     4861 aaccgcgaaa tcaaagtgag cttcttcgac aagtatggtc gtctgattgg tagcttcctg
     4921 ccggaaaaaa ccaagaaaag cgcggagatc attctggtgc agagcaagaa ctatctgaac
     4981 gaagatgtgc gtatggatac cgcgcgccgt atggaaattg cgggtctgca caacatccgt
     5041 gcgaatctgc gctactacga taagaaacac aagggcgact ttaaagagaa ggttgacgcg
     5101 attagcggct acattgacgc gctgaaccgt gcgccgagcg ttaatgatat gatgctgctg
     5161 gaagcgaaag cgcgccaact gtattatacc tgttttaacc aaatcctgga aaccagcgac
     5221 ttccagttcg aaaagcgtac caagcgtccg ccgaaagatg cgatcaatgc gtgcattagc
     5281 ttcggcaata ccctgctgta taatctattt gtgaatatta tctggaagaa gggtctggac
     5341 ccgcgctttg gcgttgttca cgcgagcaac aaacgcaatc aaagcctgaa cctggacttc
     5401 gcggacatct ttaaaccgat cgtgattgac cgcattatct tcaccatgat caataagaaa
     5461 atgctgaccc tgctgaccga ttttgaaacc agcaaccagg gtgtgtatct gagccgtgag
     5521 ggcaagaaca tcttcctgca aatgtacgag gagaagctga aaagccgcat taccatcaag
     5581 ggtaaagaga tgagctatta ccagctgctg gagagcgaag tgcagaatta caagaatttc
     5641 attctgaccg gcgaaaccta taaaccgtat aagtattatt aacgtacgtt aactttaaga
     5701 aggagaactt aagatgtatg ttatcctggt gtacgacatc caccagaagc gtgttggtaa
     5761 agcgctgaaa atctgccgta agtacctgat ccacatccaa aagagcgtgt tcgaaggcaa
     5821 catcaccgag agcaaactga aggcgctgaa agaggaactg ggtcacctga ttgacaccca
     5881 gatggatagc gtgattatct accacctgga cagcgttaag tacaccaaaa aggagcagat
     5941 cggtattgtg cagagcacca gcaatgtgat ctaactcgag tagcctagca taaccccgcg
     6001 gggcctcttc gggggtctcg cggggttttt tgctataaaa cgaaaggctc agtcgaaaga
     6061 ctgggccttt cgttttatct gctaacaaag cccgaaagga agctgagttg gctgctgcca
     6121 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     6181 tgctgaaagg aggaactata tccggactga tagctagctc agtcctaggg attatgctag
     6241 ctctagtagt ggcggggaag cttatgttcc atagcaaaaa gtcggtcagt ctcgtggctg
     6301 aaatcatgag ttccacaaaa tggctgaaat tcaaggaaaa tcaggaatct cagaaaaacg
     6361 atcgaccgac tttttcgata aaatggttgc aaaaatgaga aaaatctgat ttaatagaat
     6421 ctgaaaacag cggaaatgct gttgtcgtac tttacctaaa aggaattgaa acgtccccgc
     6481 caggttgaat ccgatatttg gaggtacgat ggaacagtct gggtgggatt gagaagagaa
     6541 aagaaaaccg ccgatcctgt ccaccgcatt actgcaaggt agtggacaag accggcggtc
     6601 ttaagttttt tggctgaagc
//
