LOCUS Exported 12551 bp DNA circular SYN 01-APR-2022 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12551) AUTHORS NYU School of Medicine TITLE Direct Submission JOURNAL Exported Apr 1, 2022 from SnapGene 6.0.2 https://www.snapgene.com FEATURES Location/Qualifiers source 1..12551 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 1..52 /label=oLM1240.LP-LM.F1 protein_bind 1..34 /allele="lox2272" /label=LoxM /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites." primer_bind complement(1..34) /label=oSP218 primer_bind complement(1..33) /label=oSP291_500bp2loxStuffer_EF1alpha_R primer_bind 1..31 /label=oSP340 primer_bind complement(4..34) /label=oSP60_Cre_Rev_ins_413 primer_bind complement(5..34) /label=oJML0019 primer_bind complement(9..36) /label=oJML0255 Lvec-HTRA1-UL50-68homol_R primer_bind complement(9..35) /label=oJML0239 Lvec-HTRA1-LIMS3homology_R primer_bind complement(9..35) /label=oJML0223 primer_bind complement(9..34) /label=oJML0254 Lvec-HTRA1-UL100-54homol_R primer_bind complement(9..34) /label=oJML0253 Lvec-HTRA1-UL150-41homol_R primer_bind complement(9..34) /label=oJML0252 Lvec-HTRA1-UL200-28homol_R primer_bind complement(9..34) /label=oJML0251 Lvec-HTRA1-UL250-14homol_R primer_bind complement(9..34) /label=oJML0250 Lvec-HTRA1-UL300-1homol_R primer_bind 12..52 /label=oSP90_correcting_CEGS_Frag2_For primer_bind complement(12..52) /label=oSP89_correcting_CEGS_Frag1_Rev primer_bind 34..54 /label=RB_065_GG primer_bind 34..53 /label=oLM878.dl5.F promoter 35..1213 /label=pEF1a primer_bind 35..64 /label=oSP257_DeletionofloxM primer_bind 35..54 /label=oLM1273.marker.R primer_bind 35..52 /label=oLM1204.PL2.F4 primer_bind complement(64..81) /label=oJML0189 primer_bind complement(96..132) /label=IRL_mAgeI-R3 primer_bind 96..132 /label=IRL_mAgeI-F3 primer_bind complement(139..162) /label=oSP66_CEGS_MMEJ_Jnctn1R primer_bind complement(201..224) /label=oSP163_EF1Alpha_Rev_alt primer_bind complement(213..234) /label=oLM1281.RgBlock.F primer_bind complement(264..284) /label=oSP351_EF1alpha_250bp_NheI_R primer_bind complement(268..286) /label=oSP290_250bp2loxStuffer_EF1alpha_R primer_bind complement(395..454) /label=oSP91_correcting_CEGS_Frag2_Rev primer_bind 508..527 /label=IRL_pEF1a-SeqF1 primer_bind complement(509..534) /label=oSP352_Ef1alpha_500bp_NheI_R primer_bind complement(509..534) /label=oSP291_500bp2loxStuffer_EF1alpha_R primer_bind complement(582..601) /label=DT1084R primer_bind 709..728 /label=DT1043F primer_bind 810..828 /label=oLM787.FAP.3.2-4.F primer_bind 814..833 /label=oRB_122_pEF1a-qPCR-F primer_bind 910..931 /label=oLM770.FAP4.F primer_bind complement(943..962) /label=oRB_123_pEF1a-qPCR-R primer_bind complement(972..989) /label=oLM773.FAP3.2.R primer_bind complement(1013..1036) /label=oSP353_Ef1alpha_1000bp_NheI_R primer_bind complement(1013..1036) /label=oSP292_1000bp2loxStuffer_EF1alpha_R primer_bind complement(1037..1059) /label=oLM788.FAP.3.2-4.R primer_bind 1068..1091 /label=oSP67_CEGS_MMEJ_Jnctn2F primer_bind 1166..1185 /label=Zwaka_Z20-149 primer_bind 1166..1185 /label=IRL_pEF1a-SeqF2 primer_bind complement(1189..1213) /label=oJML0257 EF1aProm_R primer_bind 1191..1251 /label=oJML0240 mMaroon1-EF1ahomol_F primer_bind complement(1193..1254) /label=oJML0243 EF1aProm-mMaroonhomol_R primer_bind 1193..1251 /label=oSP217 primer_bind complement(1212..1233) /label=oSP56_EF1_Alpha_Rev_small CDS 1234..1950 /codon_start=1 /label=eGFP /note="/ApEinfo_fwdcolor=#5aff2b /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /ApEinfo_revcolor=green" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind 1234..1254 /label=oJML0065 primer_bind 1234..1254 /label=oJML0173 primer_bind complement(1234..1253) /label=IRL_GFP+mOrange-SeqR1 primer_bind 1234..1252 /label=oSP210_mCherry_Ded1_F primer_bind 1234..1252 /label=oSP198_mCherry_Pab1_F primer_bind 1234..1252 /label=oLM952.750.gfp.3.F primer_bind 1270..1282 /label=oLM743.FAPv1.F1 primer_bind complement(1280..1300) /label=GENEWIZ_EGFP-N primer_bind 1291..1310 /label=IRL_eGFP-2 F primer_bind 1357..1380 /label=oRB_240-GFP-F primer_bind complement(1376..1395) /label=IRL_eGFP-2 R primer_bind 1390..1406 /label=oLM938.tetohac.gfp.F primer_bind 1450..1469 /label=IRL_eGFP-1 F primer_bind complement(1534..1557) /label=oRB_241-GFP-R primer_bind complement(1582..1601) /label=IRL_eGFP-1 R primer_bind complement(1601..1620) /label=oSP141_GFP_int primer_bind complement(1624..1641) /label=oRB_241-GFP-R primer_bind complement(1663..1686) /label=oLM800.synIXR-lox.seq.R2 primer_bind complement(1698..1718) /label=oLM939.tetohac.gfp.R primer_bind 1872..1892 /label=oLM631.human.GFP.F primer_bind 1887..1908 /label=GENEWIZ_EGFP-C-For primer_bind 1889..1908 /label=oSP236_GFP_C_F primer_bind complement(1930..1958) /label=oSP148_mCherry_Gly_Ser_R primer_bind complement(1930..1950) /label=oSP199_mCherry_R primer_bind complement(1930..1950) /label=oSP354_mCherry_NheI_R primer_bind complement(1930..1950) /label=oSP296_mCherry2loxStuffer_mCherry_bghpA_R primer_bind complement(1930..1950) /label=oSP544_GFP_Lenti_R primer_bind complement(1930..1950) /label=oSP295_2000bp2loxStuffer_mCherry_R primer_bind complement(1930..1950) /label=oSP224_2lox_LHM21_Rev primer_bind 1931..1969 /label=RB_062_Gibs primer_bind 1931..1969 /label=RB_063_Gibs primer_bind complement(1931..1954) /label=RB_066_GG primer_bind complement(1931..1952) /label=oJML0247 mScarlet-P2A8homol_R primer_bind complement(1931..1950) /label=oJML0066 primer_bind complement(1931..1950) /label=oJML0174 primer_bind complement(1935..1950) /label=GFP-ClaI-R primer_bind complement(1938..1970) /label=RB_063_Gibs primer_bind complement(1938..1970) /label=RB_062_Gibs primer_bind 1951..1973 /label=RB_067_GG CDS 1960..2013 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" primer_bind 1994..2033 /label=oSP331_BlasticidinR_F primer_bind complement(1997..2033) /label=oSP330_T2A_R CDS 2014..2412 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" primer_bind 2070..2129 /label=oSP339_Blast_BbsImut_F primer_bind complement(2070..2129) /label=oSP338_Blast_BbsImut_R primer_bind complement(2386..2445) /label=oSP332_BlasticidinR_R polyA_signal 2422..2653 /label=bGHpA /label=bGH polyA primer_bind complement(2440..2457) /label=BGHR primer_bind complement(2443..2472) /label=oSP296_mCherry2loxStuffer_mCherry_bghpA_R primer_bind 2443..2463 /label=oSP297_mCherry2loxStuffer_bghpA_F primer_bind 2443..2463 /label=oJML0007 primer_bind 2443..2462 /label=oSP381_bgH-Hyg_F primer_bind 2456..2477 /label=oLM1280.LgBlock.R primer_bind 2620..2642 /label=oLM791.FAP.5-chs4.F primer_bind 2632..2651 /label=oLM1270.bGHpa.F primer_bind complement(2633..2653) /label=oLM1272.marker.F primer_bind complement(2633..2653) /label=RB_068_GG primer_bind complement(2634..2711) /label=RB_064_Gibs primer_bind complement(2634..2711) /label=RB_064_Gibs primer_bind 2641..2668 /label=loxP-NotI-F primer_bind 2654..2688 /label=oSP282_InsLoxP protein_bind 2654..2687 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." primer_bind 2654..2687 /label=oJML0232 primer_bind 2654..2687 /label=oSP218 primer_bind complement(2654..2687) /label=oSP218 primer_bind 2654..2684 /label=oSP55_EF1_Alpha_For_ins_413 primer_bind 2654..2684 /label=oSP340 primer_bind 2654..2679 /label=oJML0256 Rvec-HTRA1-UL80homol_F primer_bind 2654..2679 /label=oJML0228 primer_bind 2654..2679 /label=oJML0224 primer_bind 2654..2672 /label=oLM731.FAP.3HPRT.F primer_bind complement(2657..2687) /label=oSP60_Cre_Rev_ins_413 primer_bind complement(2658..2687) /label=oJML0019 primer_bind 2686..2716 /label=oSP417_MmHoxA_piece16_rightvec_F primer_bind 2688..2717 /label=oSP389_pLM1050_RightVec_MmHoxa32_F primer_bind 2688..2705 /label=LM182.pRX1.ISceI.Detc misc_feature 2706..2905 /label=Rvec primer_bind 2706..2745 /label=oJML0275 pLM1050Fusion-MlyI primer_bind 2706..2727 /label=oJML0231 primer_bind complement(2710..2729) /label=oLM1147.pLM949.HO.R primer_bind complement(2733..2752) /label=oLm1205.PL2.R4 primer_bind 2866..2925 /label=oLM1090.LEU2.F primer_bind complement(2881..2905) /label=oSP390_pLM1050_RightVec_R primer_bind complement(2881..2905) /label=oLM1278.1081rlink.R primer_bind complement(2882..2905) /label=oLM988.loaders.R3 primer_bind complement(2884..2905) /label=oLM1104.1087.R primer_bind 2906..2925 /label=oLM809.HPRT.LEU2.F primer_bind 2906..2925 /label=LM91.U-to-L.F primer_bind complement(2909..2930) /label=oLM1271.1110vec.R promoter 2926..3167 /label=pLEU2 /label=pLEU2-guess primer_bind 2972..2993 /label=LM139.synIXR.nomarker.3'detc primer_bind complement(3128..3167) /label=LM134.IXR.nomarker.F CDS 3168..4262 /codon_start=1 /label=LEU2 /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG DLGGSNSTTEVGDAVAEEVKKILA" primer_bind complement(3185..3206) /label=oLM726.LEU2.internal.Rev primer_bind 3338..3352 /label=oLM635.HIS2.3detc primer_bind 3338..3352 /label=oLM439.HIS2.200.R primer_bind 3927..3946 /label=oLM1016.aG.gRNA.r.F primer_bind 4197..4218 /label=oLM727.LEU2.internal.For primer_bind 4218..4237 /label=oLM1060.LEU2.int.F primer_bind complement(4232..4264) /label=oSP377_LEU2_pLM453_R primer_bind complement(4233..4262) /label=oLM378.LEU2.R terminator 4263..4497 /label=ttLEU2 primer_bind complement(4497..4516) /label=oLM1058.aro4DLEU2.R primer_bind complement(4497..4516) /label=oLM725.erg6DLEU2.R1 primer_bind complement(4497..4516) /label=oLM356.synVI.circle.LEU2.R primer_bind complement(4498..4557) /label=oLM1091.LEU2.R primer_bind complement(4498..4516) /label=oLM810.HPRT.LEU2.R primer_bind 4524..4544 /label=oJML0229 misc_feature 4525..4724 /label=Rvec primer_bind 4525..4544 /label=oJML0232 primer_bind complement(4705..4724) /label=oJML0230 primer_bind complement(4739..4764) /label=oLM1105.1092.R CDS complement(4839..5654) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 5071..5089 /label=oLM71.IXR#7.R primer_bind complement(5079..5099) /label=oLM1096.seq4.R primer_bind complement(5142..5163) /label=oKB12 primer_bind 5188..5228 /label=oLM1075.pBAC02.F1 primer_bind complement(5188..5228) /label=oLM1074.pBAC02.R1 primer_bind complement(5456..5476) /label=oLM69.IXR#7.F-vec primer_bind 5541..5560 /label=oLM632.kanR.R primer_bind 5618..5637 /label=oLM796.synIXR-lox.R promoter complement(5655..5757) /label=pCat /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" primer_bind complement(5720..5741) /label=oLM928.leftvec1.F3 primer_bind complement(5722..5741) /label=oLM932.cmr.F primer_bind 5724..5747 /label=oLM837.Gen9.seq.R primer_bind 5743..5782 /label=oLM1077.pLM453.F2 primer_bind complement(5743..5782) /label=oLM1076.pBAC02.R2 primer_bind 5767..5796 /label=oJML0070 primer_bind 5767..5786 /label=oLM926.leftvec1.F2 misc_feature 5777..6280 /label=CEN/ARS /note="S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence" primer_bind complement(5870..5894) /label=oLM718.CEN.ultramer.R2 primer_bind complement(5898..5919) /label=oLM580.pigCen.cspcr.R primer_bind 5964..6005 /label=oJML0096 primer_bind 5976..6001 /label=oLM628.416.R primer_bind complement(5976..5997) /label=oJML0094 primer_bind 5998..6026 /label=oJML0095 primer_bind 6258..6301 /label=oLM1079.pBAC02.F3 primer_bind complement(6258..6301) /label=oLM1078.pLM453.R3 primer_bind 6261..6277 /label=oLM458.YFR051C.200.F misc_feature 6388..6735 /label=redF rep_origin 7131..7745 /label=oriV /note="origin of replication for the bacterial F plasmid" primer_bind 7712..7743 /label=oSP91_correcting_CEGS_Frag2_Rev misc_feature 7748..7802 /label=OriV rep_origin 7821..8040 /label=ori2 /note="secondary origin of replication for the bacterial F plasmid; also known as oriS" primer_bind 7927..7938 /label=IRL_mAgeI-F3 CDS 8131..8886 /codon_start=1 /gene="repE" /product="replication initiation protein for the bacterial F plasmid" /label=repE /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM RLSYIEKKKGRQTTHIVFSFRDITSMTTG" primer_bind 8651..8694 /label=oLM1081.pBAC02.F4 primer_bind complement(8651..8694) /label=oLM1080.pBAC02.R4 misc_feature 8889..9139 /gene="incC" /label=incC /note="incompatibility region of the bacterial F plasmid" CDS 9465..10640 /codon_start=1 /gene="sopA" /product="partitioning protein for the bacterial F plasmid" /label=sopA /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR" CDS 10640..11611 /codon_start=1 /gene="sopB" /product="partitioning protein for the bacterial F plasmid" /label=sopB /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP" primer_bind complement(10789..10799) /label=IRL_eGFP-1 F primer_bind 11522..11566 /label=oLM1083.pBAC02.F5 primer_bind complement(11522..11566) /label=oLM1082.pBAC02.R5 misc_feature 11684..12157 /label=sopC/parC primer_bind 12258..12278 /label=oLM1093.seq1.F primer_bind 12294..12317 /label=oLM1103.1084.F misc_feature 12334..12533 /label=Lvec primer_bind 12334..12363 /label=oLM983.loaders&acc.F1 primer_bind 12334..12359 /label=oJML0222 primer_bind 12334..12358 /label=oLM1279.LgBlock.F primer_bind 12494..12533 /label=oJML0275 pLM1050Fusion-MlyI primer_bind 12495..12514 /label=oLM1146.pLM949.HO.F primer_bind complement(12508..12533) /label=oLM984.loaders.R1 primer_bind complement(12508..12533) /label=oLM989.acc.R4 primer_bind complement(12514..12551) /label=oSP384_pLM1050_LeftVec_MmHoxa1_R primer_bind complement(12514..12551) /label=oSP418_MmHoxA_piece17_leftvec_R primer_bind join(12528..12551,1..46) /label=RB_061_Gibs primer_bind join(12528..12551,1..46) /label=RB_061_Gibs primer_bind 12534..12551 /label=LM182.pRX1.ISceI.Detc primer_bind join(12551,1..31) /label=oSP55_EF1_Alpha_For_ins_413 primer_bind join(12551,1..23) /label=oSP282_InsLoxP ORIGIN 1 ataacttcgt ataggatact ttatacgaag ttatggctcc ggtgcccgtc agtgggcaga 61 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc 121 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt 181 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg 241 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct 301 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat 361 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg 421 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg 481 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa 541 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca 601 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc 661 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg 721 gtagtctcaa gctggccggc ctgctctggt gcctagtctc gcgccgccgt gtatcgcccc 781 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct 841 tcccggccct actgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg 901 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc 961 cacggagtac cgggcgctgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc 1021 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga 1081 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga 1141 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt 1201 tcaggtgtcg tgatctctag aagctgggta ccaatggtga gcaagggcga ggagctgttc 1261 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 1321 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 1381 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 1441 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 1501 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 1561 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 1621 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 1681 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 1741 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 1801 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 1861 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 1921 atcactctcg gcatggacga gctgtacaag ggcagtggag agggcagagg aagtctgcta 1981 acatgcggtg acgtcgagga gaatcctggc ccaatggcca agcctttgtc tcaagaagaa 2041 tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc tgaggactac 2101 agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat 2161 cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca 2221 gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc 2281 tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag 2341 gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg 2401 tgggagggct aagaattcta actagagctc gctgatcagc ctcgactgtg ccttctagtt 2461 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc 2521 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt 2581 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gagaatagca 2641 ggcatgctgg ggaataactt cgtatagcat acattatacg aagttattag ggataacagg 2701 gtaattttag ggtagcatca ggaatctgaa ccctcagaaa gtggggatcc cgggtataga 2761 cctttatctg cggttcaagt taggcataag gctgcatgct accttgtcac acctacactg 2821 ctcgaagtaa atatgggaag cgtgcgacct ggctccaggc gttccgcgcc gccacgtgtt 2881 cgttaactgt tgattggtgg cacatagagg tcgcctgacg catatacctt tttcaactga 2941 aaaattggga gaaaaaggaa aggtgagagc gccggaaccg gcttttcata tagaatagag 3001 aagcgttcat gactaaatgc ttgcatcaca atacttgaag ttgacaatat tatttaagga 3061 cctattgttt tttccaatag gtggttagca atcgtcttac tttctaactt ttcttacctt 3121 ttacatttca gcaatatata tatatatttc aaggatatac cattctaatg tctgccccta 3181 agaagatcgt cgttttgcca ggtgaccacg ttggtcaaga aatcacagcc gaagccatta 3241 aggttcttaa agctatttct gatgttcgtt ccaatgtcaa gttcgatttc gaaaatcatt 3301 taattggtgg tgctgctatc gatgctacag gtgttccact tccagatgag gcgctggaag 3361 cctccaagaa ggctgatgcc gttttgttag gtgctgtggg tggtcctaaa tggggtaccg 3421 gtagtgttag acctgaacaa ggtttactaa aaatccgtaa agaacttcaa ttgtacgcca 3481 acttaagacc atgtaacttt gcatccgact ctcttttaga cttatctcca atcaagccac 3541 aatttgctaa aggtactgac ttcgttgttg tcagagaatt agtgggaggt atttactttg 3601 gtaagagaaa ggaagacgat ggtgatggtg tcgcttggga tagtgaacaa tacaccgttc 3661 cagaagtgca aagaatcaca agaatggccg ctttcatggc cctacaacat gagccaccat 3721 tgcctatttg gtccttggat aaagctaatg ttttggcctc ttcaagatta tggagaaaaa 3781 ctgtggagga aaccatcaag aacgaattcc ctacattgaa ggttcaacat caattgattg 3841 attctgccgc catgatccta gttaagaacc caacccacct aaatggtatt ataatcacca 3901 gcaacatgtt tggtgatatc atctccgatg aagcctccgt tatcccaggt tccttgggtt 3961 tgttgccatc tgcgtccttg gcctctttgc cagacaagaa caccgcattt ggtttgtacg 4021 aaccatgcca cggttctgct ccagatttgc caaagaataa ggtcaaccct atcgccacta 4081 tcttgtctgc tgcaatgatg ttgaaattgt cattgaactt gcctgaagaa ggtaaggcca 4141 ttgaagatgc agttaaaaag gttttggatg caggtatcag aactggtgat ttaggtggtt 4201 ccaacagtac caccgaagtc ggtgatgctg tcgccgaaga agttaagaaa atccttgctt 4261 aaaaagattc tcttttttta tgatatttgt acataaactt tataaatgaa attcataata 4321 gaaacgacac gaaattacaa aatggaatat gttcataggg tagacgaaac tatatacgca 4381 atctacatac atttatcaag aaggagaaaa aggaggatgt aaaggaatac aggtaagcaa 4441 attgatacta atggctcaac gtgataagga aaaagaattg cactttaaca ttaatattga 4501 caaggaggag ggcaccgcgg ccgcccaact acgcagccga tcaacaacag aaacgcgacg 4561 gtcgagatga ggaacgcaca ctggagcctt tatcagatgg tgttgatttc aattactccg 4621 gagcgtgatg cggatcaaag taaccgcgat ggaaggtggg ggactcgaaa tagatccgtt 4681 cttagccctg cgaaacatct actgtacacc acatgcaaca gccacaattc tcatgtttga 4741 cagcttatca tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcaa 4801 ccaggcgttt aagggcacca ataactgcct taaaaaaatt agaaaaactc atcgagcatc 4861 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt 4921 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat 4981 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa 5041 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa 5101 agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa 5161 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagccag acgaaatacg 5221 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact 5281 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct 5341 gtttttccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc 5401 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta 5461 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc 5521 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac 5581 ccatataaat cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt 5641 tgaatatggc tcattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg 5701 tccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg 5761 tccctgggtc cttttcatca cgtgctataa aaataattat aatttaaatt ttttaatata 5821 aatatataaa ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt 5881 tccgaagatg taaaagactc tagggggatc gccaacaaat actacctttt atcttgctct 5941 tcctgctctc aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac 6001 gaaaatcctg tgattttaca ttttacttat cgttaatcga atgtatatct atttaatctg 6061 cttttcttgt ctaataaata tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct 6121 ttgtttattt ttttttcttc attccgtaac tcttctacct tctttattta ctttctaaaa 6181 tccaaataca aaacataaaa ataaataaac acagagtaaa ttcccaaatt attccatcat 6241 taaaagatac gaggcgcgtg taagttacag gcaagcgatc ttttcgccaa aagttggccc 6301 agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg 6361 tcacaggtat ttattcgcga taagctcatg gagcggcgta accgtcgcac aggaaggaca 6421 gagaaagcgc ggatctggga agtgacggac agaacggtca ggacctggat tggggaggcg 6481 gttgccgccg ctgctgctga cggtgtgacg ttctctgttc cggtcacacc acatacgttc 6541 cgccattcct atgcgatgca catgctgtat gccggtatac cgctgaaagt tctgcaaagc 6601 ctgatgggac ataagtccat cagttcaacg gaagtctaca cgaaggtttt tgcgctggat 6661 gtggctgccc ggcaccgggt gcagtttgcg atgccggagt ctgatgcggt tgcgatgctg 6721 aaacaattat cctgagaata aatgccttgg cctttatatg gaaatgtgga actgagtgga 6781 tatgctgttt ttgtctgtta aacagagaag ctggctgtta tccactgaga agcgagcgaa 6841 acagtcggga aaatctccca ttatcgtaga gatccgcatt attaatctca ggagcctgtg 6901 tagcgtttat aggaagtagt gttctgtcat gatgcctgca agcggtaacg aaaacgattt 6961 gaatatgcct tcaggaacaa tagaaatctt cgtgcggtgt tacgttgaag tggagcggat 7021 tatgtcagca atggacagaa caacctaatg aacacagaac catgatgtgg tctgtccttt 7081 tacagccagt agtgctcgcc gcagtcgagc gacagggcga agccctcgag ctggttgccc 7141 tcgccgctgg gctggcggct gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc 7201 cgtgtgcgag acaccgcggc cggccgccgg cgttgtggat acctcgcgga aaacttggcc 7261 ctcactgaca gatgaggggc ggacgttgac acttgagggg ccgactcacc cggcgcggcg 7321 ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt ggagctggac agcctcgcaa 7381 atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga tgatgtggac aagcctgggg 7441 ataagtgccc tgcggtattg acacttgagg ggcgcgacta ctgacagatg aggggcgcga 7501 tccttgacac ttgaggggca gagtgctgac agatgagggg cgcacctatt gacatttgag 7561 gggctgtcca caggcagaaa atccagcatt tgcaagggtt tccgcccgtt tttcggccac 7621 cgctaacctg tcttttaacc tgcttttaaa ccaatattta taaaccttgt ttttaaccag 7681 ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc ttctcgaacc 7741 ctcccggtcg agtgagcgag gaagcaccag ggaacagcac ttatatattc tgcttacaca 7801 cgatgcctga aaaaacttcc cttggggtta tccacttatc cacggggata tttttataat 7861 tatttttttt atagttttta gatcttcttt tttagagcgc cttgtaggcc tttatccatg 7921 ctggttctag agaaggtgtt gtgacaaatt gccctttcag tgtgacaaat caccctcaaa 7981 tgacagtcct gtctgtgaca aattgccctt aaccctgtga caaattgccc tcagaagaag 8041 ctgttttttc acaaagttat ccctgcttat tgactctttt ttatttagtg tgacaatcta 8101 aaaacttgtc acacttcaca tggatctgtc atggcggaaa cagcggttat caatcacaag 8161 aaacgtaaaa atagcccgcg aatcgtccag tcaaacgacc tcactgaggc ggcatatagt 8221 ctctcccggg atcaaaaacg tatgctgtat ctgttcgttg accagatcag aaaatctgat 8281 ggcaccctac aggaacatga cggtatctgc gagatccatg ttgctaaata tgctgaaata 8341 ttcggattga cctctgcgga agccagtaag gatatacggc aggcattgaa gagtttcgcg 8401 gggaaggaag tggtttttta tcgccctgaa gaggatgccg gcgatgaaaa aggctatgaa 8461 tcttttcctt ggtttatcaa acgtgcgcac agtccatcca gagggcttta cagtgtacat 8521 atcaacccat atctcattcc cttctttatc gggttacaga accggtttac gcagtttcgg 8581 cttagtgaaa caaaagaaat caccaatccg tatgccatgc gtttatacga atccctgtgt 8641 cagtatcgta agccggatgg ctcaggcatt gtctctctga aaatcgactg gatcatagag 8701 cgttaccagc tgcctcaaag ttaccagcgt atgcctgact tccgccgccg cttcctgcag 8761 gtctgtgtta atgagatcaa cagcagaact ccaatgcgcc tctcatacat tgagaaaaag 8821 aaaggccgcc agacgactca tatcgtattt tccttccgcg atatcacttc catgacgaca 8881 ggatagtctg agggttatct gtcacagatt tgagggtggt tcgtcacatt tgttctgacc 8941 tactgagggt aatttgtcac agttttgctg tttccttcag cctgcatgga ttttctcata 9001 ctttttgaac tgtaattttt aaggaagcca aatttgaggg cagtttgtca cagttgattt 9061 ccttctcttt cccttcgtca tgtgacctga tatcgggggt tagttcgtca tcattgatga 9121 gggttgatta tcacagttta ttactctgaa ttggctatcc gcgtgtgtac ctctacctgg 9181 agtttttccc acggtggata tttcttcttg cgctgagcgt aagagctatc tgacagaaca 9241 gttcttcttt gcttcctcgc cagttcgctc gctatgctcg gttacacggc tgcggcgagc 9301 gctagtgata ataagtgact gaggtatgtg ctcttcttat ctccttttgt agtgttgctc 9361 ttattttaaa caactttgcg gttttttgat gactttgcga ttttgttgtt gctttgcagt 9421 aaattgcaag atttaataaa aaaacgcaaa gcaatgatta aaggatgttc agaatgaaac 9481 tcatggaaac acttaaccag tgcataaacg ctggtcatga aatgacgaag gctatcgcca 9541 ttgcacagtt taatgatgac agcccggaag cgaggaaaat aacccggcgc tggagaatag 9601 gtgaagcagc ggatttagtt ggggtttctt ctcaggctat cagagatgcc gagaaagcag 9661 ggcgactacc gcacccggat atggaaattc gaggacgggt tgagcaacgt gttggttata 9721 caattgaaca aattaatcat atgcgtgatg tgtttggtac gcgattgcga cgtgctgaag 9781 acgtatttcc accggtgatc ggggttgctg cccataaagg tggcgtttac aaaacctcag 9841 tttctgttca tcttgctcag gatctggctc tgaaggggct acgtgttttg ctcgtggaag 9901 gtaacgaccc ccagggaaca gcctcaatgt atcacggatg ggtaccagat cttcatattc 9961 atgcagaaga cactctcctg cctttctatc ttggggaaaa ggacgatgtc acttatgcaa 10021 taaagcccac ttgctggccg gggcttgaca ttattccttc ctgtctggct ctgcaccgta 10081 ttgaaactga gttaatgggc aaatttgatg aaggtaaact gcccaccgat ccacacctga 10141 tgctccgact ggccattgaa actgttgctc atgactatga tgtcatagtt attgacagcg 10201 cgcctaacct gggtatcggc acgattaatg tcgtatgtgc tgctgatgtg ctgattgttc 10261 ccacgcctgc tgagttgttt gactacacct ccgcactgca gtttttcgat atgcttcgtg 10321 atctgctcaa gaacgttgat cttaaagggt tcgagcctga tgtacgtatt ttgcttacca 10381 aatacagcaa tagcaatggc tctcagtccc cgtggatgga ggagcaaatt cgggatgcct 10441 ggggaagcat ggttctaaaa aatgttgtac gtgaaacgga tgaagttggt aaaggtcaga 10501 tccggatgag aactgttttt gaacaggcca ttgatcaacg ctcttcaact ggtgcctgga 10561 gaaatgctct ttctatttgg gaacctgtct gcaatgaaat tttcgatcgt ctgattaaac 10621 cacgctggga gattagataa tgaagcgtgc gcctgttatt ccaaaacata cgctcaatac 10681 tcaaccggtt gaagatactt cgttatcgac accagctgcc ccgatggtgg attcgttaat 10741 tgcgcgcgta ggagtaatgg ctcgcggtaa tgccattact ttgcctgtat gtggtcggga 10801 tgtgaagttt actcttgaag tgctccgggg tgatagtgtt gagaagacct ctcgggtatg 10861 gtcaggtaat gaacgtgacc aggagctgct tactgaggac gcactggatg atctcatccc 10921 ttcttttcta ctgactggtc aacagacacc ggcgttcggt cgaagagtat ctggtgtcat 10981 agaaattgcc gatgggagtc gccgtcgtaa agctgctgca cttaccgaaa gtgattatcg 11041 tgttctggtt ggcgagctgg atgatgagca gatggctgca ttatccagat tgggtaacga 11101 ttatcgccca acaagtgctt atgaacgtgg tcagcgttat gcaagccgat tgcagaatga 11161 atttgctgga aatatttctg cgctggctga tgcggaaaat atttcacgta agattattac 11221 ccgctgtatc aacaccgcca aattgcctaa atcagttgtt gctctttttt ctcaccccgg 11281 tgaactatct gcccggtcag gtgatgcact tcaaaaagcc tttacagata aagaggaatt 11341 acttaagcag caggcatcta accttcatga gcagaaaaaa gctggggtga tatttgaagc 11401 tgaagaagtt atcactcttt taacttctgt gcttaaaacg tcatctgcat caagaactag 11461 tttaagctca cgacatcagt ttgctcctgg agcgacagta ttgtataagg gcgataaaat 11521 ggtgcttaac ctggacaggt cccgtgttcc aactgagtgt atagagaaaa ttgaggccat 11581 tcttaaggaa cttgaaaagc cagcaccctg atgcgacctc gttttagtct acgtttatct 11641 gtctttactt aatgtccttt gttacaggcc agaaagcata actggcctga atattctctc 11701 tgggcccact gttccacttg tatcgtcggt ctgataatca gactgggacc acggtcccac 11761 tcgtatcgtc ggtctgatta ttagtctggg accacggtcc cactcgtatc gtcggtctga 11821 ttattagtct gggaccacgg tcccactcgt atcgtcggtc tgataatcag actgggacca 11881 cggtcccact cgtatcgtcg gtctgattat tagtctggga ccatggtccc actcgtatcg 11941 tcggtctgat tattagtctg ggaccacggt cccactcgta tcgtcggtct gattattagt 12001 ctggaaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac cacggtccca 12061 ctcgtatcgt cggtctgatt attagtctgg gaccacgatc ccactcgtgt tgtcggtctg 12121 attatcggtc tgggaccacg gtcccacttg tattgtcgat cagactatca gcgtgagact 12181 acgattccat caatgcctgt caagggcaag tattgacatg tcgtcgtaac ctgtagaacg 12241 gagtaacctc ggtgtgcggt tgtatgcctg cggtggattg ctgctgtgtc ctgcttatcc 12301 acaacatttt gcgcacggtt atgtggacgg gcctaaggta gctaccaata tttagtttct 12361 aagccttgcg acagacctcc cacttagatt gccacgcata gagctagcga gtcagcgaaa 12421 agcatgacgc gctttcaagc gtggcgagta tgtgaaccaa ggcttcggac aggactatat 12481 acttaggttt gatctcgccc cgagaactgt aaacctcaac atttatagat tattagggat 12541 aacagggtaa t //