LOCUS       Exported               14183 bp DNA     circular SYN 01-APR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 1, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..52
                     /label=oLM1240.LP-LM.F1
     misc_recomb     1..34
                     /standard_name="loxM"
                     /label=loxM
                     /note="Lox2272 (LoxM)"
     primer_bind     complement(1..34)
                     /label=oSP218
     primer_bind     complement(1..33)
                     /label=oSP291_500bp2loxStuffer_EF1alpha_R
     primer_bind     1..31
                     /label=oSP340
     primer_bind     1..26
                     /label=oJML0395
     primer_bind     1..15
                     /label=oJML0187
     primer_bind     complement(4..34)
                     /label=oSP60_Cre_Rev_ins_413
     primer_bind     complement(5..34)
                     /label=oJML0019
     primer_bind     complement(8..34)
                     /label=oJML0389
     primer_bind     complement(9..36)
                     /label=oJML0255 Lvec-HTRA1-UL50-68homol_R
     primer_bind     complement(9..36)
                     /label=oJML0255  Lvec-HTRA1-UL50-68homol_R
     primer_bind     complement(9..35)
                     /label=oJML0239 Lvec-HTRA1-LIMS3homology_R
     primer_bind     complement(9..35)
                     /label=oJML0223
     primer_bind     complement(9..35)
                     /label=oJML0239  Lvec-HTRA1-LIMS3homology_R
     primer_bind     complement(9..34)
                     /label=oJML0254 Lvec-HTRA1-UL100-54homol_R
     primer_bind     complement(9..34)
                     /label=oJML0253 Lvec-HTRA1-UL150-41homol_R
     primer_bind     complement(9..34)
                     /label=oJML0252 Lvec-HTRA1-UL200-28homol_R
     primer_bind     complement(9..34)
                     /label=oJML0251 Lvec-HTRA1-UL250-14homol_R
     primer_bind     complement(9..34)
                     /label=oJML0250 Lvec-HTRA1-UL300-1homol_R
     primer_bind     complement(9..34)
                     /label=oJML0250  Lvec-HTRA1-UL300-1homol_R
     primer_bind     complement(9..34)
                     /label=oJML0251  Lvec-HTRA1-UL250-14homol_R
     primer_bind     complement(9..34)
                     /label=oJML0252  Lvec-HTRA1-UL200-28homol_R
     primer_bind     complement(9..34)
                     /label=oJML0253  Lvec-HTRA1-UL150-41homol_R
     primer_bind     complement(9..34)
                     /label=oJML0254  Lvec-HTRA1-UL100-54homol_R
     primer_bind     12..52
                     /label=oSP90_correcting_CEGS_Frag2_For
     primer_bind     complement(12..52)
                     /label=oSP89_correcting_CEGS_Frag1_Rev
     primer_bind     34..54
                     /label=RB_065_GG
     primer_bind     34..53
                     /label=oLM878.dl5.F
     promoter        35..1213
                     /label=pEF1a
     primer_bind     35..64
                     /label=oSP257_DeletionofloxM
     primer_bind     35..54
                     /label=oLM1273.marker.R
     primer_bind     35..52
                     /label=oLM1204.PL2.F4
     primer_bind     35..52
                     /label=oLM1257.PL1to1050.F
     primer_bind     35..52
                     /label=oJML0049
     primer_bind     complement(64..81)
                     /label=oJML0189
     primer_bind     complement(70..86)
                     /label=oSP287_50bp2loxStuffer_Ef1alpha_R
     primer_bind     complement(96..132)
                     /label=IRL_mAgeI-R3
     primer_bind     96..132
                     /label=IRL_mAgeI-F3
     primer_bind     complement(139..162)
                     /label=oSP66_CEGS_MMEJ_Jnctn1R
     primer_bind     complement(201..224)
                     /label=oSP163_EF1Alpha_Rev_alt
     primer_bind     complement(213..234)
                     /label=oLM1281.RgBlock.F
     primer_bind     complement(264..284)
                     /label=oSP351_EF1alpha_250bp_NheI_R
     intron          266..1204
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(268..286)
                     /label=oSP290_250bp2loxStuffer_EF1alpha_R
     primer_bind     complement(395..454)
                     /label=oSP91_correcting_CEGS_Frag2_Rev
     primer_bind     508..527
                     /label=IRL_pEF1a-SeqF1
     primer_bind     complement(509..534)
                     /label=oSP352_Ef1alpha_500bp_NheI_R
     primer_bind     complement(509..534)
                     /label=oSP291_500bp2loxStuffer_EF1alpha_R
     primer_bind     complement(582..601)
                     /label=DT1084R
     primer_bind     656..666
                     /label=oRB_254_phPGK1-F1
     primer_bind     709..728
                     /label=DT1043F
     primer_bind     810..828
                     /label=oLM787.FAP.3.2-4.F
     primer_bind     814..833
                     /label=oRB_122_pEF1a-qPCR-F
     primer_bind     910..931
                     /label=oLM770.FAP4.F
     primer_bind     complement(943..962)
                     /label=oRB_123_pEF1a-qPCR-R
     primer_bind     complement(972..989)
                     /label=oLM773.FAP3.2.R
     primer_bind     979..997
                     /label=oRB_270_pEF1a-F
     primer_bind     complement(1013..1036)
                     /label=oSP353_Ef1alpha_1000bp_NheI_R
     primer_bind     complement(1013..1036)
                     /label=oSP292_1000bp2loxStuffer_EF1alpha_R
     primer_bind     complement(1037..1059)
                     /label=oLM788.FAP.3.2-4.R
     primer_bind     1068..1091
                     /label=oSP67_CEGS_MMEJ_Jnctn2F
     primer_bind     1166..1185
                     /label=Zwaka_Z20-149
     primer_bind     1166..1185
                     /label=IRL_pEF1a-SeqF2
     primer_bind     1166..1185
                     /label=oJML0402
     primer_bind     1166..1185
                     /label=pEF1a-SeqF2
     primer_bind     complement(1189..1213)
                     /label=oJML0257 EF1aProm_R
     primer_bind     1191..1251
                     /label=oJML0240 mMaroon1-EF1ahomol_F
     primer_bind     complement(1193..1254)
                     /label=oJML0243 EF1aProm-mMaroonhomol_R
     primer_bind     complement(1193..1254)
                     /label=oJML0243  EF1aProm-mMaroonhomol_R
     primer_bind     1193..1251
                     /label=oSP217
     primer_bind     complement(1212..1233)
                     /label=oSP56_EF1_Alpha_Rev_small
     CDS             1234..1950
                     /codon_start=1
                     /label=eGFP
                     /note="/ApEinfo_fwdcolor=#5aff2b
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     1234..1254
                     /label=oJML0065
     primer_bind     1234..1254
                     /label=oJML0173
     primer_bind     complement(1234..1253)
                     /label=IRL_GFP+mOrange-SeqR1
     primer_bind     complement(1234..1253)
                     /label=GFP-SeqR1
     primer_bind     1234..1252
                     /label=oSP210_mCherry_Ded1_F
     primer_bind     1234..1252
                     /label=oSP198_mCherry_Pab1_F
     primer_bind     1234..1252
                     /label=oLM952.750.gfp.3.F
     primer_bind     1234..1251
                     /label=oSP294_2000bp2loxStuffer_mCherry_F
     primer_bind     1270..1282
                     /label=oLM743.FAPv1.F1
     primer_bind     complement(1280..1300)
                     /label=GENEWIZ_EGFP-N
     primer_bind     1291..1310
                     /label=IRL_eGFP-2 F
     primer_bind     1357..1380
                     /label=oRB_240-GFP-F
     primer_bind     complement(1376..1395)
                     /label=IRL_eGFP-2 R
     primer_bind     1390..1406
                     /label=oLM938.tetohac.gfp.F
     primer_bind     1450..1469
                     /label=IRL_eGFP-1 F
     primer_bind     complement(1534..1557)
                     /label=oRB_241-GFP-R
     primer_bind     complement(1582..1601)
                     /label=IRL_eGFP-1 R
     primer_bind     complement(1601..1620)
                     /label=oSP141_GFP_int
     primer_bind     complement(1624..1641)
                     /label=oRB_241-GFP-R
     primer_bind     complement(1663..1686)
                     /label=oLM800.synIXR-lox.seq.R2
     primer_bind     complement(1698..1718)
                     /label=oLM939.tetohac.gfp.R
     primer_bind     1872..1892
                     /label=oLM631.human.GFP.F
     primer_bind     1887..1908
                     /label=GENEWIZ_EGFP-C-For
     primer_bind     1889..1908
                     /label=oSP236_GFP_C_F
     primer_bind     complement(1930..1958)
                     /label=oSP148_mCherry_Gly_Ser_R
     primer_bind     complement(1930..1950)
                     /label=oSP199_mCherry_R
     primer_bind     complement(1930..1950)
                     /label=oSP354_mCherry_NheI_R
     primer_bind     complement(1930..1950)
                     /label=oSP296_mCherry2loxStuffer_mCherry_bghpA_R
     primer_bind     complement(1930..1950)
                     /label=oSP544_GFP_Lenti_R
     primer_bind     complement(1930..1950)
                     /label=oSP295_2000bp2loxStuffer_mCherry_R
     primer_bind     complement(1930..1950)
                     /label=oSP224_2lox_LHM21_Rev
     primer_bind     1931..1969
                     /label=RB_062_Gibs
     primer_bind     1931..1969
                     /label=RB_063_Gibs
     primer_bind     complement(1931..1954)
                     /label=RB_066_GG
     primer_bind     complement(1931..1952)
                     /label=oJML0247 mScarlet-P2A8homol_R
     primer_bind     complement(1931..1950)
                     /label=oJML0066
     primer_bind     complement(1931..1950)
                     /label=oJML0174
     primer_bind     complement(1935..1950)
                     /label=GFP-ClaI-R
     primer_bind     complement(1938..1970)
                     /label=RB_063_Gibs
     primer_bind     complement(1938..1970)
                     /label=RB_062_Gibs
     primer_bind     1951..1973
                     /label=RB_067_GG
     CDS             1960..2013
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     1994..2033
                     /label=oSP331_BlasticidinR_F
     primer_bind     complement(1997..2033)
                     /label=oSP330_T2A_R
     CDS             2014..2412
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     2046..2066
                     /label=oRB_267_BSD-R
     primer_bind     2070..2129
                     /label=oSP339_Blast_BbsImut_F
     primer_bind     complement(2070..2129)
                     /label=oSP338_Blast_BbsImut_R
     primer_bind     2173..2192
                     /label=oRB_266_BSD-R
     primer_bind     complement(2229..2248)
                     /label=oRB_265_BSD-F
     primer_bind     complement(2386..2445)
                     /label=oSP332_BlasticidinR_R
     polyA_signal    2422..2653
                     /label=bGHpA
                     /label=bGH polyA
     primer_bind     complement(2440..2457)
                     /label=BGHR
     primer_bind     complement(2440..2457)
                     /label=GENEWIZ_BGHR
     primer_bind     complement(2443..2472)
                     /label=oSP296_mCherry2loxStuffer_mCherry_bghpA_R
     primer_bind     2443..2463
                     /label=oSP297_mCherry2loxStuffer_bghpA_F
     primer_bind     2443..2463
                     /label=oJML0007
     primer_bind     2443..2462
                     /label=oSP381_bgH-Hyg_F
     primer_bind     2456..2477
                     /label=oLM1280.LgBlock.R
     primer_bind     2620..2642
                     /label=oLM791.FAP.5-chs4.F
     primer_bind     2632..2651
                     /label=oLM1270.bGHpa.F
     primer_bind     complement(2633..2653)
                     /label=oLM1272.marker.F
     primer_bind     complement(2633..2653)
                     /label=RB_068_GG
     primer_bind     complement(2634..2711)
                     /label=RB_064_Gibs
     primer_bind     complement(2634..2711)
                     /label=RB_064_Gibs
     primer_bind     complement(2636..2653)
                     /label=oLM1258.PL1to1050.R
     primer_bind     2641..2668
                     /label=loxP-NotI-F
     primer_bind     2654..2688
                     /label=oSP282_InsLoxP
     misc_recomb     2654..2687
                     /standard_name="loxP"
                     /label=loxP
                     /note="bacteriophage P1 LoxP site"
     primer_bind     2654..2687
                     /label=oJML0232
     primer_bind     2654..2687
                     /label=oSP218
     primer_bind     complement(2654..2687)
                     /label=oSP218
     primer_bind     2654..2684
                     /label=oSP55_EF1_Alpha_For_ins_413
     primer_bind     2654..2684
                     /label=oSP340
     primer_bind     2654..2679
                     /label=oJML0256 Rvec-HTRA1-UL80homol_F
     primer_bind     2654..2679
                     /label=oJML0228
     primer_bind     2654..2679
                     /label=oJML0224
     primer_bind     2654..2679
                     /label=oJML0395
     primer_bind     2654..2679
                     /label=oJML0256  Rvec-HTRA1-UL80homol_F
     primer_bind     2654..2672
                     /label=oLM731.FAP.3HPRT.F
     primer_bind     complement(2657..2687)
                     /label=oSP60_Cre_Rev_ins_413
     primer_bind     complement(2658..2687)
                     /label=oJML0019
     primer_bind     complement(2661..2687)
                     /label=oJML0389
     primer_bind     complement(2672..2688)
                     /label=oJML0188
     primer_bind     2686..2716
                     /label=oSP417_MmHoxA_piece16_rightvec_F
     primer_bind     2688..2717
                     /label=oSP389_pLM1050_RightVec_MmHoxa32_F
     primer_bind     2688..2705
                     /label=LM182.pRX1.ISceI.Detc
     misc_feature    2706..2905
                     /label=Rvec
     primer_bind     2706..2745
                     /label=oJML0275 pLM1050Fusion-MlyI
     primer_bind     2706..2727
                     /label=oJML0231
     primer_bind     complement(2710..2729)
                     /label=oLM1147.pLM949.HO.R
     primer_bind     complement(2733..2752)
                     /label=oLm1205.PL2.R4
     primer_bind     2830..2849
                     /label=oRB_252_Rvec_gRNA_1F
     primer_bind     2866..2925
                     /label=oLM1090.LEU2.F
     primer_bind     complement(2881..2905)
                     /label=oSP390_pLM1050_RightVec_R
     primer_bind     complement(2881..2905)
                     /label=oLM1278.1081rlink.R
     primer_bind     complement(2882..2905)
                     /label=oLM988.loaders.R3
     primer_bind     complement(2884..2905)
                     /label=oLM1104.1087.R
     primer_bind     complement(2884..2905)
                     /label=oJML0225
     primer_bind     complement(2884..2905)
                     /label=oLM993.acc.R6
     primer_bind     complement(2884..2905)
                     /label=oLM1261.Rvec.R
     primer_bind     2906..2925
                     /label=oLM809.HPRT.LEU2.F
     primer_bind     2906..2925
                     /label=LM91.U-to-L.F
     primer_bind     complement(2909..2930)
                     /label=oLM1271.1110vec.R
     promoter        2926..3179
                     /label=LEU2 promoter
                     /label=pLEU2-guess
                     /note="From Leslie's pLM1050 annotation. Shorter than the 
                     SnapGene default one."
     primer_bind     2972..2993
                     /label=LM139.synIXR.nomarker.3'detc
     primer_bind     complement(3128..3167)
                     /label=LM134.IXR.nomarker.F
     CDS             3168..4262
                     /codon_start=1
                     /label=LEU2
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(3185..3206)
                     /label=oLM726.LEU2.internal.Rev
     primer_bind     3338..3352
                     /label=oLM635.HIS2.3detc
     primer_bind     3338..3352
                     /label=oLM439.HIS2.200.R
     primer_bind     3927..3946
                     /label=oLM1016.aG.gRNA.r.F
     primer_bind     4197..4218
                     /label=oLM727.LEU2.internal.For
     primer_bind     4218..4237
                     /label=oLM1060.LEU2.int.F
     primer_bind     complement(4232..4264)
                     /label=oSP377_LEU2_pLM453_R
     primer_bind     complement(4233..4262)
                     /label=oLM378.LEU2.R
     terminator      4263..4497
                     /label=ttLEU2
     primer_bind     complement(4497..4516)
                     /label=oLM1058.aro4DLEU2.R
     primer_bind     complement(4497..4516)
                     /label=oLM725.erg6DLEU2.R1
     primer_bind     complement(4497..4516)
                     /label=oLM356.synVI.circle.LEU2.R
     primer_bind     complement(4498..4557)
                     /label=oLM1091.LEU2.R
     primer_bind     complement(4498..4516)
                     /label=oLM810.HPRT.LEU2.R
     primer_bind     4524..4544
                     /label=oJML0229
     misc_feature    4525..4724
                     /label=Rvec
     primer_bind     4525..4544
                     /label=oJML0232
     primer_bind     complement(4705..4724)
                     /label=oJML0230
     primer_bind     complement(4739..4764)
                     /label=oLM1105.1092.R
     CDS             complement(4839..5654)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5071..5089
                     /label=oLM71.IXR#7.R
     primer_bind     complement(5079..5099)
                     /label=oLM1096.seq4.R
     primer_bind     complement(5142..5163)
                     /label=oKB12
     primer_bind     5188..5228
                     /label=oLM1075.pBAC02.F1
     primer_bind     complement(5188..5228)
                     /label=oLM1074.pBAC02.R1
     primer_bind     complement(5456..5476)
                     /label=oLM69.IXR#7.F-vec
     primer_bind     5541..5560
                     /label=oLM632.kanR.R
     primer_bind     5618..5637
                     /label=oLM796.synIXR-lox.R
     promoter        complement(5655..5757)
                     /label=pCat
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(5720..5741)
                     /label=oLM928.leftvec1.F3
     primer_bind     complement(5720..5741)
                     /label=oLM928.leftvec1.F3_
     primer_bind     complement(5722..5741)
                     /label=oLM932.cmr.F
     primer_bind     5724..5747
                     /label=oLM837.Gen9.seq.R
     primer_bind     5743..5782
                     /label=oLM1077.pLM453.F2
     primer_bind     complement(5743..5782)
                     /label=oLM1076.pBAC02.R2
     primer_bind     5767..5796
                     /label=oJML0070
     primer_bind     5767..5786
                     /label=oLM926.leftvec1.F2
     misc_feature    5777..6280
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     complement(5870..5894)
                     /label=oLM718.CEN.ultramer.R2
     primer_bind     complement(5898..5919)
                     /label=oLM580.pigCen.cspcr.R
     primer_bind     5964..6005
                     /label=oJML0096
     primer_bind     5976..6001
                     /label=oLM628.416.R
     primer_bind     complement(5976..5997)
                     /label=oJML0094
     primer_bind     5998..6026
                     /label=oJML0095
     primer_bind     6258..6301
                     /label=oLM1079.pBAC02.F3
     primer_bind     complement(6258..6301)
                     /label=oLM1078.pLM453.R3
     primer_bind     6261..6277
                     /label=oLM458.YFR051C.200.F
     primer_bind     complement(6264..6280)
                     /label=oJML0054
     misc_feature    6388..6735
                     /label=redF
     rep_origin      7131..7745
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     primer_bind     7712..7743
                     /label=oSP91_correcting_CEGS_Frag2_Rev
     misc_feature    7748..7802
                     /label=OriV
     rep_origin      7821..8040
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     primer_bind     7927..7938
                     /label=IRL_mAgeI-F3
     CDS             8131..8886
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     primer_bind     8651..8694
                     /label=oLM1081.pBAC02.F4
     primer_bind     complement(8651..8694)
                     /label=oLM1080.pBAC02.R4
     misc_feature    8889..9139
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             9465..10640
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             10640..11611
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     primer_bind     complement(10789..10799)
                     /label=IRL_eGFP-1 F
     primer_bind     11522..11566
                     /label=oLM1083.pBAC02.F5
     primer_bind     complement(11522..11566)
                     /label=oLM1082.pBAC02.R5
     misc_feature    11684..12157
                     /label=sopC/parC
     primer_bind     12258..12278
                     /label=oLM1093.seq1.F
     primer_bind     12286..12342
                     /label=oJML0396  dTK-TU_PayloadIntegration_F
     primer_bind     12286..12342
                     /label=oJML0396
     primer_bind     12294..12324
                     /label=oLM1257.PL1to1050.F
     primer_bind     12294..12317
                     /label=oLM1103.1084.F
     promoter        12343..12826
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     12343..12360
                     /label=oLM1178.PL2.F1
     primer_bind     12345..12366
                     /label=oJML0278 pPGK-LPmod_F
     primer_bind     12347..12366
                     /label=oLM1202.PL2.v2F1
     primer_bind     complement(12362..12379)
                     /label=oJML0387 hPGKp-Seq_R
     primer_bind     complement(12384..12403)
                     /label=oRB_254_phPGK1-F1
     primer_bind     12637..12646
                     /label=oSP257_DeletionofloxM
     CDS             12832..13824
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995).
                     Recoded to remove several restriction sites."
                     /label=Delta-TKmod
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     12832..12849
                     /label=oJML0137 deltaTK-Purohomol_F
     primer_bind     12832..12849
                     /label=oJML0137
     primer_bind     13256..13269
                     /label=oSP236_GFP_C_F
     primer_bind     13256..13269
                     /label=GENEWIZ_EGFP-C-For
     primer_bind     complement(13805..13824)
                     /label=oJML0129 TK-P2Ahomool_R
     primer_bind     complement(13805..13824)
                     /label=oJML0129
     polyA_signal    13828..13949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13861..13892)
                     /label=LM255.NeoR
     primer_bind     complement(13865..13884)
                     /label=GENEWIZ_SV40pA-R
     primer_bind     complement(13865..13884)
                     /label=LM244.SV40pA-R
     primer_bind     13903..13927
                     /label=oLM860.FAP.polyA.seq.R
     primer_bind     13904..13929
                     /label=LM198.HPRT.I2.R3
     primer_bind     13904..13929
                     /label=oSP256_SV40pA_F
     primer_bind     13918..13939
                     /label=LM202.HPRT.gblock.R
     primer_bind     complement(13926..13951)
                     /label=oLM1181.PL2.R2
     primer_bind     complement(13932..13991)
                     /label=oJML0397 dTK-TU_PayloadIntegration_R
     primer_bind     complement(13932..13991)
                     /label=oJML0397
     primer_bind     complement(13932..13949)
                     /label=oLM953.750.gfp.3.R
     misc_feature    13966..14165
                     /label=Lvec
     primer_bind     complement(13966..14005)
                     /label=oLM1258.PL1to1050.R
     primer_bind     13966..13995
                     /label=oLM983.loaders&acc.F1
     primer_bind     13966..13995
                     /label=oLM.1259.Lvec.F
     primer_bind     13966..13991
                     /label=oJML0222
     primer_bind     13966..13990
                     /label=oLM1279.LgBlock.F
     primer_bind     13993..14035
                     /label=oLM1178.PL2.F1
     primer_bind     14054..14073
                     /label=oRB_250_Lvec_gRNA_1F
     primer_bind     14126..14165
                     /label=oJML0275 pLM1050Fusion-MlyI
     primer_bind     14127..14146
                     /label=oLM1146.pLM949.HO.F
     primer_bind     complement(14140..14165)
                     /label=oLM984.loaders.R1
     primer_bind     complement(14140..14165)
                     /label=oLM989.acc.R4
     primer_bind     complement(14146..14183)
                     /label=oSP384_pLM1050_LeftVec_MmHoxa1_R
     primer_bind     complement(14146..14183)
                     /label=oSP418_MmHoxA_piece17_leftvec_R
     primer_bind     join(14160..14183,1..46)
                     /label=RB_061_Gibs
     primer_bind     join(14160..14183,1..46)
                     /label=RB_061_Gibs
     primer_bind     14166..14183
                     /label=LM182.pRX1.ISceI.Detc
     primer_bind     join(14178..14183,1..34)
                     /label=oLM1202.PL2.v2F1
     primer_bind     join(14183,1..31)
                     /label=oSP55_EF1_Alpha_For_ins_413
     primer_bind     join(14183,1..23)
                     /label=oSP282_InsLoxP
ORIGIN
        1 ataacttcgt ataggatact ttatacgaag ttatggctcc ggtgcccgtc agtgggcaga
       61 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
      121 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
      181 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
      241 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
      301 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat
      361 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
      421 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg
      481 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
      541 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
      601 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
      661 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
      721 gtagtctcaa gctggccggc ctgctctggt gcctagtctc gcgccgccgt gtatcgcccc
      781 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
      841 tcccggccct actgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
      901 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
      961 cacggagtac cgggcgctgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc
     1021 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     1081 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     1141 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     1201 tcaggtgtcg tgatctctag aagctgggta ccaatggtga gcaagggcga ggagctgttc
     1261 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     1321 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1381 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     1441 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1501 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1561 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1621 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1681 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     1741 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1801 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     1861 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1921 atcactctcg gcatggacga gctgtacaag ggcagtggag agggcagagg aagtctgcta
     1981 acatgcggtg acgtcgagga gaatcctggc ccaatggcca agcctttgtc tcaagaagaa
     2041 tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc tgaggactac
     2101 agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat
     2161 cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca
     2221 gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc
     2281 tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag
     2341 gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg
     2401 tgggagggct aagaattcta actagagctc gctgatcagc ctcgactgtg ccttctagtt
     2461 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     2521 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     2581 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gagaatagca
     2641 ggcatgctgg ggaataactt cgtatagcat acattatacg aagttattag ggataacagg
     2701 gtaattttag ggtagcatca ggaatctgaa ccctcagaaa gtggggatcc cgggtataga
     2761 cctttatctg cggttcaagt taggcataag gctgcatgct accttgtcac acctacactg
     2821 ctcgaagtaa atatgggaag cgtgcgacct ggctccaggc gttccgcgcc gccacgtgtt
     2881 cgttaactgt tgattggtgg cacatagagg tcgcctgacg catatacctt tttcaactga
     2941 aaaattggga gaaaaaggaa aggtgagagc gccggaaccg gcttttcata tagaatagag
     3001 aagcgttcat gactaaatgc ttgcatcaca atacttgaag ttgacaatat tatttaagga
     3061 cctattgttt tttccaatag gtggttagca atcgtcttac tttctaactt ttcttacctt
     3121 ttacatttca gcaatatata tatatatttc aaggatatac cattctaatg tctgccccta
     3181 agaagatcgt cgttttgcca ggtgaccacg ttggtcaaga aatcacagcc gaagccatta
     3241 aggttcttaa agctatttct gatgttcgtt ccaatgtcaa gttcgatttc gaaaatcatt
     3301 taattggtgg tgctgctatc gatgctacag gtgttccact tccagatgag gcgctggaag
     3361 cctccaagaa ggctgatgcc gttttgttag gtgctgtggg tggtcctaaa tggggtaccg
     3421 gtagtgttag acctgaacaa ggtttactaa aaatccgtaa agaacttcaa ttgtacgcca
     3481 acttaagacc atgtaacttt gcatccgact ctcttttaga cttatctcca atcaagccac
     3541 aatttgctaa aggtactgac ttcgttgttg tcagagaatt agtgggaggt atttactttg
     3601 gtaagagaaa ggaagacgat ggtgatggtg tcgcttggga tagtgaacaa tacaccgttc
     3661 cagaagtgca aagaatcaca agaatggccg ctttcatggc cctacaacat gagccaccat
     3721 tgcctatttg gtccttggat aaagctaatg ttttggcctc ttcaagatta tggagaaaaa
     3781 ctgtggagga aaccatcaag aacgaattcc ctacattgaa ggttcaacat caattgattg
     3841 attctgccgc catgatccta gttaagaacc caacccacct aaatggtatt ataatcacca
     3901 gcaacatgtt tggtgatatc atctccgatg aagcctccgt tatcccaggt tccttgggtt
     3961 tgttgccatc tgcgtccttg gcctctttgc cagacaagaa caccgcattt ggtttgtacg
     4021 aaccatgcca cggttctgct ccagatttgc caaagaataa ggtcaaccct atcgccacta
     4081 tcttgtctgc tgcaatgatg ttgaaattgt cattgaactt gcctgaagaa ggtaaggcca
     4141 ttgaagatgc agttaaaaag gttttggatg caggtatcag aactggtgat ttaggtggtt
     4201 ccaacagtac caccgaagtc ggtgatgctg tcgccgaaga agttaagaaa atccttgctt
     4261 aaaaagattc tcttttttta tgatatttgt acataaactt tataaatgaa attcataata
     4321 gaaacgacac gaaattacaa aatggaatat gttcataggg tagacgaaac tatatacgca
     4381 atctacatac atttatcaag aaggagaaaa aggaggatgt aaaggaatac aggtaagcaa
     4441 attgatacta atggctcaac gtgataagga aaaagaattg cactttaaca ttaatattga
     4501 caaggaggag ggcaccgcgg ccgcccaact acgcagccga tcaacaacag aaacgcgacg
     4561 gtcgagatga ggaacgcaca ctggagcctt tatcagatgg tgttgatttc aattactccg
     4621 gagcgtgatg cggatcaaag taaccgcgat ggaaggtggg ggactcgaaa tagatccgtt
     4681 cttagccctg cgaaacatct actgtacacc acatgcaaca gccacaattc tcatgtttga
     4741 cagcttatca tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcaa
     4801 ccaggcgttt aagggcacca ataactgcct taaaaaaatt agaaaaactc atcgagcatc
     4861 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     4921 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
     4981 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     5041 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     5101 agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     5161 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagccag acgaaatacg
     5221 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     5281 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     5341 gtttttccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     5401 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     5461 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     5521 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     5581 ccatataaat cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt
     5641 tgaatatggc tcattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     5701 tccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg
     5761 tccctgggtc cttttcatca cgtgctataa aaataattat aatttaaatt ttttaatata
     5821 aatatataaa ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt
     5881 tccgaagatg taaaagactc tagggggatc gccaacaaat actacctttt atcttgctct
     5941 tcctgctctc aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac
     6001 gaaaatcctg tgattttaca ttttacttat cgttaatcga atgtatatct atttaatctg
     6061 cttttcttgt ctaataaata tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct
     6121 ttgtttattt ttttttcttc attccgtaac tcttctacct tctttattta ctttctaaaa
     6181 tccaaataca aaacataaaa ataaataaac acagagtaaa ttcccaaatt attccatcat
     6241 taaaagatac gaggcgcgtg taagttacag gcaagcgatc ttttcgccaa aagttggccc
     6301 agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg
     6361 tcacaggtat ttattcgcga taagctcatg gagcggcgta accgtcgcac aggaaggaca
     6421 gagaaagcgc ggatctggga agtgacggac agaacggtca ggacctggat tggggaggcg
     6481 gttgccgccg ctgctgctga cggtgtgacg ttctctgttc cggtcacacc acatacgttc
     6541 cgccattcct atgcgatgca catgctgtat gccggtatac cgctgaaagt tctgcaaagc
     6601 ctgatgggac ataagtccat cagttcaacg gaagtctaca cgaaggtttt tgcgctggat
     6661 gtggctgccc ggcaccgggt gcagtttgcg atgccggagt ctgatgcggt tgcgatgctg
     6721 aaacaattat cctgagaata aatgccttgg cctttatatg gaaatgtgga actgagtgga
     6781 tatgctgttt ttgtctgtta aacagagaag ctggctgtta tccactgaga agcgagcgaa
     6841 acagtcggga aaatctccca ttatcgtaga gatccgcatt attaatctca ggagcctgtg
     6901 tagcgtttat aggaagtagt gttctgtcat gatgcctgca agcggtaacg aaaacgattt
     6961 gaatatgcct tcaggaacaa tagaaatctt cgtgcggtgt tacgttgaag tggagcggat
     7021 tatgtcagca atggacagaa caacctaatg aacacagaac catgatgtgg tctgtccttt
     7081 tacagccagt agtgctcgcc gcagtcgagc gacagggcga agccctcgag ctggttgccc
     7141 tcgccgctgg gctggcggct gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc
     7201 cgtgtgcgag acaccgcggc cggccgccgg cgttgtggat acctcgcgga aaacttggcc
     7261 ctcactgaca gatgaggggc ggacgttgac acttgagggg ccgactcacc cggcgcggcg
     7321 ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt ggagctggac agcctcgcaa
     7381 atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga tgatgtggac aagcctgggg
     7441 ataagtgccc tgcggtattg acacttgagg ggcgcgacta ctgacagatg aggggcgcga
     7501 tccttgacac ttgaggggca gagtgctgac agatgagggg cgcacctatt gacatttgag
     7561 gggctgtcca caggcagaaa atccagcatt tgcaagggtt tccgcccgtt tttcggccac
     7621 cgctaacctg tcttttaacc tgcttttaaa ccaatattta taaaccttgt ttttaaccag
     7681 ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc ttctcgaacc
     7741 ctcccggtcg agtgagcgag gaagcaccag ggaacagcac ttatatattc tgcttacaca
     7801 cgatgcctga aaaaacttcc cttggggtta tccacttatc cacggggata tttttataat
     7861 tatttttttt atagttttta gatcttcttt tttagagcgc cttgtaggcc tttatccatg
     7921 ctggttctag agaaggtgtt gtgacaaatt gccctttcag tgtgacaaat caccctcaaa
     7981 tgacagtcct gtctgtgaca aattgccctt aaccctgtga caaattgccc tcagaagaag
     8041 ctgttttttc acaaagttat ccctgcttat tgactctttt ttatttagtg tgacaatcta
     8101 aaaacttgtc acacttcaca tggatctgtc atggcggaaa cagcggttat caatcacaag
     8161 aaacgtaaaa atagcccgcg aatcgtccag tcaaacgacc tcactgaggc ggcatatagt
     8221 ctctcccggg atcaaaaacg tatgctgtat ctgttcgttg accagatcag aaaatctgat
     8281 ggcaccctac aggaacatga cggtatctgc gagatccatg ttgctaaata tgctgaaata
     8341 ttcggattga cctctgcgga agccagtaag gatatacggc aggcattgaa gagtttcgcg
     8401 gggaaggaag tggtttttta tcgccctgaa gaggatgccg gcgatgaaaa aggctatgaa
     8461 tcttttcctt ggtttatcaa acgtgcgcac agtccatcca gagggcttta cagtgtacat
     8521 atcaacccat atctcattcc cttctttatc gggttacaga accggtttac gcagtttcgg
     8581 cttagtgaaa caaaagaaat caccaatccg tatgccatgc gtttatacga atccctgtgt
     8641 cagtatcgta agccggatgg ctcaggcatt gtctctctga aaatcgactg gatcatagag
     8701 cgttaccagc tgcctcaaag ttaccagcgt atgcctgact tccgccgccg cttcctgcag
     8761 gtctgtgtta atgagatcaa cagcagaact ccaatgcgcc tctcatacat tgagaaaaag
     8821 aaaggccgcc agacgactca tatcgtattt tccttccgcg atatcacttc catgacgaca
     8881 ggatagtctg agggttatct gtcacagatt tgagggtggt tcgtcacatt tgttctgacc
     8941 tactgagggt aatttgtcac agttttgctg tttccttcag cctgcatgga ttttctcata
     9001 ctttttgaac tgtaattttt aaggaagcca aatttgaggg cagtttgtca cagttgattt
     9061 ccttctcttt cccttcgtca tgtgacctga tatcgggggt tagttcgtca tcattgatga
     9121 gggttgatta tcacagttta ttactctgaa ttggctatcc gcgtgtgtac ctctacctgg
     9181 agtttttccc acggtggata tttcttcttg cgctgagcgt aagagctatc tgacagaaca
     9241 gttcttcttt gcttcctcgc cagttcgctc gctatgctcg gttacacggc tgcggcgagc
     9301 gctagtgata ataagtgact gaggtatgtg ctcttcttat ctccttttgt agtgttgctc
     9361 ttattttaaa caactttgcg gttttttgat gactttgcga ttttgttgtt gctttgcagt
     9421 aaattgcaag atttaataaa aaaacgcaaa gcaatgatta aaggatgttc agaatgaaac
     9481 tcatggaaac acttaaccag tgcataaacg ctggtcatga aatgacgaag gctatcgcca
     9541 ttgcacagtt taatgatgac agcccggaag cgaggaaaat aacccggcgc tggagaatag
     9601 gtgaagcagc ggatttagtt ggggtttctt ctcaggctat cagagatgcc gagaaagcag
     9661 ggcgactacc gcacccggat atggaaattc gaggacgggt tgagcaacgt gttggttata
     9721 caattgaaca aattaatcat atgcgtgatg tgtttggtac gcgattgcga cgtgctgaag
     9781 acgtatttcc accggtgatc ggggttgctg cccataaagg tggcgtttac aaaacctcag
     9841 tttctgttca tcttgctcag gatctggctc tgaaggggct acgtgttttg ctcgtggaag
     9901 gtaacgaccc ccagggaaca gcctcaatgt atcacggatg ggtaccagat cttcatattc
     9961 atgcagaaga cactctcctg cctttctatc ttggggaaaa ggacgatgtc acttatgcaa
    10021 taaagcccac ttgctggccg gggcttgaca ttattccttc ctgtctggct ctgcaccgta
    10081 ttgaaactga gttaatgggc aaatttgatg aaggtaaact gcccaccgat ccacacctga
    10141 tgctccgact ggccattgaa actgttgctc atgactatga tgtcatagtt attgacagcg
    10201 cgcctaacct gggtatcggc acgattaatg tcgtatgtgc tgctgatgtg ctgattgttc
    10261 ccacgcctgc tgagttgttt gactacacct ccgcactgca gtttttcgat atgcttcgtg
    10321 atctgctcaa gaacgttgat cttaaagggt tcgagcctga tgtacgtatt ttgcttacca
    10381 aatacagcaa tagcaatggc tctcagtccc cgtggatgga ggagcaaatt cgggatgcct
    10441 ggggaagcat ggttctaaaa aatgttgtac gtgaaacgga tgaagttggt aaaggtcaga
    10501 tccggatgag aactgttttt gaacaggcca ttgatcaacg ctcttcaact ggtgcctgga
    10561 gaaatgctct ttctatttgg gaacctgtct gcaatgaaat tttcgatcgt ctgattaaac
    10621 cacgctggga gattagataa tgaagcgtgc gcctgttatt ccaaaacata cgctcaatac
    10681 tcaaccggtt gaagatactt cgttatcgac accagctgcc ccgatggtgg attcgttaat
    10741 tgcgcgcgta ggagtaatgg ctcgcggtaa tgccattact ttgcctgtat gtggtcggga
    10801 tgtgaagttt actcttgaag tgctccgggg tgatagtgtt gagaagacct ctcgggtatg
    10861 gtcaggtaat gaacgtgacc aggagctgct tactgaggac gcactggatg atctcatccc
    10921 ttcttttcta ctgactggtc aacagacacc ggcgttcggt cgaagagtat ctggtgtcat
    10981 agaaattgcc gatgggagtc gccgtcgtaa agctgctgca cttaccgaaa gtgattatcg
    11041 tgttctggtt ggcgagctgg atgatgagca gatggctgca ttatccagat tgggtaacga
    11101 ttatcgccca acaagtgctt atgaacgtgg tcagcgttat gcaagccgat tgcagaatga
    11161 atttgctgga aatatttctg cgctggctga tgcggaaaat atttcacgta agattattac
    11221 ccgctgtatc aacaccgcca aattgcctaa atcagttgtt gctctttttt ctcaccccgg
    11281 tgaactatct gcccggtcag gtgatgcact tcaaaaagcc tttacagata aagaggaatt
    11341 acttaagcag caggcatcta accttcatga gcagaaaaaa gctggggtga tatttgaagc
    11401 tgaagaagtt atcactcttt taacttctgt gcttaaaacg tcatctgcat caagaactag
    11461 tttaagctca cgacatcagt ttgctcctgg agcgacagta ttgtataagg gcgataaaat
    11521 ggtgcttaac ctggacaggt cccgtgttcc aactgagtgt atagagaaaa ttgaggccat
    11581 tcttaaggaa cttgaaaagc cagcaccctg atgcgacctc gttttagtct acgtttatct
    11641 gtctttactt aatgtccttt gttacaggcc agaaagcata actggcctga atattctctc
    11701 tgggcccact gttccacttg tatcgtcggt ctgataatca gactgggacc acggtcccac
    11761 tcgtatcgtc ggtctgatta ttagtctggg accacggtcc cactcgtatc gtcggtctga
    11821 ttattagtct gggaccacgg tcccactcgt atcgtcggtc tgataatcag actgggacca
    11881 cggtcccact cgtatcgtcg gtctgattat tagtctggga ccatggtccc actcgtatcg
    11941 tcggtctgat tattagtctg ggaccacggt cccactcgta tcgtcggtct gattattagt
    12001 ctggaaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac cacggtccca
    12061 ctcgtatcgt cggtctgatt attagtctgg gaccacgatc ccactcgtgt tgtcggtctg
    12121 attatcggtc tgggaccacg gtcccacttg tattgtcgat cagactatca gcgtgagact
    12181 acgattccat caatgcctgt caagggcaag tattgacatg tcgtcgtaac ctgtagaacg
    12241 gagtaacctc ggtgtgcggt tgtatgcctg cggtggattg ctgctgtgtc ctgcttatcc
    12301 acaacatttt gcgcacggtt atgtctcaga gacgcctgca ggggggttgg ggttgcgcct
    12361 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
    12421 acgcagcggc gccgaccctg ggcctcgcac attcttcacg tccgttcgca gcgtcacccg
    12481 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
    12541 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctgac
    12601 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
    12661 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggctggg aaggggcggt
    12721 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
    12781 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttggag gatgcccacg
    12841 ctactgcggg tttatataga cggtccccac gggatgggga aaaccaccac cacgcaactg
    12901 ctggtggccc tgggttcgcg cgacgatatc gtctacgtac ccgagccgat gacttactgg
    12961 cgggtgctgg gggcttccga gacaatcgcg aacatctaca ccacacaaca ccgcctcgac
    13021 cagggtgaga tatccgccgg ggacgcggcg gtggtaatga caagcgccca gataacaatg
    13081 gggatgcctt atgccgtgac cgacgccgtt ctggctcctc atatcggggg ggaggctggg
    13141 agctcacatg ccccgccccc ggccctcacc ctcatcttcg accgccatcc catcgccgcc
    13201 ctcctgtgct accccgccgc gcgctacctt atgggcagca tgacccccca ggccgtgctg
    13261 gcgttcgtgg ccctcatccc gccgaccttg cccggcacca acatcgtgct tggggccctt
    13321 ccggaggaca gacacatcga ccgcctggcc aaacgccagc gccccggcga gcggctggac
    13381 ctggctatgc tggctgcgat tcgccgcgtt tacgggctac ttgccaatac ggtgcggtat
    13441 ctgcaatgcg gcgggtcgtg gcgggaggac tggggacagc tttcggggac cgccgtgccg
    13501 ccccagggtg ccgagcccca gagcaacgcg ggcccacgac cccatatcgg ggacacgtta
    13561 tttaccctgt ttcgggcccc cgagttgctg gcccccaacg gcgacctgta taacgtgttt
    13621 gcctgggcct tggacgtctt ggccaaacgc ctccgttcca tgcacgtctt tatcctggat
    13681 tacgaccaat cgcccgccgg ctgccgggac gccctgctgc aacttacctc cgggatggtc
    13741 cagacccacg tcaccacccc cggctccata ccgacgatat gcgacctggc gcgcacgttt
    13801 gcccgggaga tgggggaggc taactaaaac ttgtttattg cagcttataa tggttacaaa
    13861 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    13921 ggtttgtcca aactcatcaa tgtatcttac ctgcagggac gggcctaagg tagctaccaa
    13981 tatttagttt ctaagccttg cgacagacct cccacttaga ttgccacgca tagagctagc
    14041 gagtcagcga aaagcatgac gcgctttcaa gcgtggcgag tatgtgaacc aaggcttcgg
    14101 acaggactat atacttaggt ttgatctcgc cccgagaact gtaaacctca acatttatag
    14161 attattaggg ataacagggt aat
//
