LOCUS       Exported               10429 bp ds-DNA     circular SYN 15-OCT-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10429)
  AUTHORS   Yvonne Nygard
  TITLE     Direct Submission
  JOURNAL   Exported Apr 4, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10429
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     20..58
                     /label=YN1
                     /note="color: black; sequence: 
                     TTggtctccctgaatgtgcttcagtattacattttttgccttcaacgc"
     promoter        24..123
                     /label=tRNA Phe Promoter
                     /note="color: #ffab26"
     tRNA            124..214
                     /label=tRNA Phe
                     /note="color: #ff70cb; direction: RIGHT"
     misc_feature    215..284
                     /label=HDV Ribozyme
                     /note="color: #85cfff"
     primer_bind     complement(245..284)
                     /label=MS12_Pdr12_sgRNA2_R
                     /note="color: black; sequence: 
                     gactagccttattttaacttgctatttctagctctaaaacTTATACTTAGACATCATTT
                     Cagtcccattcgccacccgaaggtgttgcccagccggcgcc"
     primer_bind     complement(245..284)
                     /label=MS2_Ade2_sgRNA_1_R
                     /note="color: black; sequence: 
                     gactagccttattttaacttgctatttctagctctaaaacTCCTAATATACCAACTGTT
                     Cagtcccattcgccacccgaaggtgttgcccagccggcgcc"
     protein_bind    complement(join(281..284,286..291))
                     /label=BsmBI
                     /label=BsmBI(1)
                     /note="This reverse directional feature has 2 segments:
                        1: 281 .. 284 / #e72e00
                        2: 286 .. 291 / #e72e00"
     promoter        292..421
                     /label=BBa_J72163 GlpT Promoter
                     /note="color: #9eff91"
     RBS             422..457
                     /label=sfGFP Ribosome Binding Site
                     /note="color: #9eff91"
     CDS             458..1174
                     /label=sfGFP
                     /note="color: #34ff03; direction: RIGHT"
     primer_bind     865..884
                     /label=MS21_GFP
                     /note="color: black; sequence: CCTGGGCCATAAGCTGGAAT"
     terminator      1175..1303
                     /label=BBa_B0015 Terminator
                     /note="color: #9eff91"
     primer_bind     1274..1350
                     /label=MS11_Pdr12_sgRNA2_F
                     /note="color: black; sequence: 
                     ggcgccggctgggcaacaccttcgggtggcgaatgggactGAAATGATGTCTAAGTATA
                     Agttttagagctagaaatagcaagttaaaataaggctagtc"
     primer_bind     1274..1350
                     /label=MS1_Ade2_sgRNA_1_F
                     /note="color: black; sequence: 
                     ggcgccggctgggcaacaccttcgggtggcgaatgggactGAACAGTTGGTATATTAGG
                     Agttttagagctagaaatagcaagttaaaataaggctagtc"
     protein_bind    join(1304..1309,1311..1314)
                     /label=BsmBI
                     /note="This feature has 2 segments:
                        1: 1304 .. 1309 / #00ff15
                        2: 1311 .. 1314 / #00ff15"
     misc_RNA        1311..1390
                     /label=sgRNA
                     /note="color: #00ffff; direction: RIGHT"
     terminator      1391..1465
                     /label=tSNR52 terminator
                     /label=tSNR52
                     /note="color: #ff7b81"
     primer_bind     complement(1432..1469)
                     /label=YN2
                     /note="color: black; sequence: 
                     TTggtctcatcagtatccactagacagaagtttgcgttccatactgt"
     misc_feature    1466..1490
                     /label=ConS scar
                     /note="color: #fffd6c"
     protein_bind    1466..1469
                     /label=BsmBI
                     /note="color: #00ff15"
     protein_bind    1491..1494
                     /label=BsaI
                     /note="color: #00ff2c"
     promoter        1495..2194
                     /label=ScPGK1 Promoter
                     /note="color: #00a1ee"
     protein_bind    2200..2203
                     /label=BsaI
                     /note="color: #00ff2c"
     CDS             2204..6304
                     /label=Cas9
                     /note="color: #00ffff; direction: RIGHT"
     primer_bind     complement(2803..2822)
                     /label=MS18_Cas9_1
                     /note="color: black; sequence: CATCGACTCCGCTTGCATTG"
     primer_bind     3934..3953
                     /label=MS19_Cas9_2
                     /note="color: black; sequence: GGAGATTAGCGGGGTCGAAG"
     primer_bind     5170..5179
                     /label=MS15_Pdr12_check_R
                     /note="color: black; sequence: AATAAGCACCTGCCGTGGTG"
     primer_bind     complement(5869..5888)
                     /label=MS20_Cas9_3
                     /note="color: black; sequence: AGGGGAGAGCCAGCTCATTA"
     sig_peptide     6314..6364
                     /label=2x NLS Tag
                     /note="color: #00ffff; direction: RIGHT"
     protein_bind    6367..6370
                     /label=BsaI
                     /note="color: #00ff2c"
     terminator      6380..6604
                     /label=ScPGK1 Terminator
                     /note="color: #ff8eff"
     primer_bind     6547..6573
                     /label=YN4
                     /note="color: black; sequence: ttcgctcctcttttaatgcctttatgc"
     protein_bind    6605..6608
                     /label=BsaI
                     /note="ApEinfo_fwdcolor=#00FF2C
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#E72E00"
                     /note="color: #00ff2c"
     misc_feature    6609..6633
                     /label=ConE scar
                     /note="color: #fffd6c"
     protein_bind    6609..6612
                     /label=BsmBI
                     /note="color: #00ff15"
     protein_bind    6649..6652
                     /label=BsaI
                     /note="ApEinfo_fwdcolor=#00FF2C
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#E72E00"
                     /note="color: #00ff2c"
     promoter        6657..7033
                     /label=AgTEF Promoter
                     /note="ApEinfo_fwdcolor=#B29990
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#B29990"
                     /note="color: #b29990"
     CDS             7036..7845
                     /label=KanR
                     /note="ApEinfo_fwdcolor=#E490FF
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#E490FF"
                     /note="color: #e490ff; direction: RIGHT"
     primer_bind     complement(7812..7832)
                     /label=MS17_KanF
                     /note="color: black; sequence: tcgagcatcaaatgaaactgc"
     terminator      7846..8047
                     /label=AgTEF Terminator
                     /note="ApEinfo_fwdcolor=#B29990
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#B29990"
                     /note="color: #b29990"
     protein_bind    8048..8051
                     /label=BsaI
                     /note="color: #00ff2c"
     rep_origin      8052..8168
                     /label=CEN6
                     /note="color: #bda661"
     rep_origin      8169..8559
                     /label=ARS4
                     /note="color: #9a969b"
     primer_bind     complement(8175..8197)
                     /label=YN5
                     /note="color: black; sequence: gtatttgttggcgatccccctag"
     protein_bind    8560..8563
                     /label=BsaI
                     /note="color: #00ff2c"
     terminator      complement(8572..8668)
                     /label=AmpR Terminator
                     /note="color: #ff6666; direction: LEFT"
     CDS             complement(8669..9526)
                     /label=AmpR
                     /note="color: #9f4240; direction: LEFT"
     promoter        complement(9527..9657)
                     /label=AmpR Promoter
                     /note="color: #ff6666; direction: LEFT"
     rep_origin      complement(9658..10421)
                     /direction=LEFT
                     /label=ColE1
                     /note="color: #7f7f7f"
ORIGIN
        1 ccctgaattc gcatctagac tgaatgtgct tcagtattac attttttgcc ttcaacgcct
       61 tgattgttct atttttgcta ataataaatc tatttcatcg gactaaaagt ccattagttg
      121 taagcggatt tagctcagtt gggagagcgc cagactgaag aaaaacttcg gtcaagtcat
      181 ctggaggtcc tgtgttcgat ccacagaatt cgcagatggc cggcatggtc ccagcctcct
      241 cgctggcgcc ggctgggcaa caccttcggg tggcgaatgg gacttgagac ggaaagtgaa
      301 acgtgatttc atgcgtcatt ttgaacattt tgtaaatctt atttaataat gtgtgcggca
      361 attcacattt aatttatgaa tgttttctta acatcgcggc aactcaagaa acggcaggtt
      421 cggatcttag ctactagaga aagaggagaa atactagatg cgtaaaggcg aagagctgtt
      481 cactggtgtc gtccctattc tggtggaact ggatggtgat gtcaacggtc ataagttttc
      541 cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa ctgacgctga agttcatctg
      601 tactactggt aaactgccgg ttccttggcc gactctggta acgacgctga cttatggtgt
      661 tcagtgcttt gctcgttatc cggaccatat gaagcagcat gacttcttca agtccgccat
      721 gccggaaggc tatgtgcagg aacgcacgat ttcctttaag gatgacggca cgtacaaaac
      781 gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac cgcattgagc tgaaaggcat
      841 tgactttaaa gaggacggca atatcctggg ccataagctg gaatacaatt ttaacagcca
      901 caatgtttac atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt ttaaaattcg
      961 ccacaacgtg gaggatggca gcgtgcagct ggctgatcac taccagcaaa acactccaat
     1021 cggtgatggt cctgttctgc tgccagacaa tcactatctg agcacgcaaa gcgttctgtc
     1081 taaagatccg aacgagaaac gcgatcatat ggttctgctg gagttcgtaa ccgcagcggg
     1141 catcacgcat ggtatggatg aactgtacaa atgaccaggc atcaaataaa acgaaaggct
     1201 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1261 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atacgtctca gttttagagc
     1321 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     1381 cggtgctttt tttatttttt gtcactattg ttatgtaaaa tgccacctct gacagtatgg
     1441 aacgcaaact tctgtctagt ggatactgaa ctggccgata attgcagacg aacggtgagt
     1501 aaggaaagag tgaggaacta tcgcatacct gcatttaaag atgccgattt gggcgcgaat
     1561 cctttatttt ggcttcaccc tcatactatt atcagggcca gaaaaaggaa gtgtttccct
     1621 ccttcttgaa ttgatgttac cctcataaag cacgtggcct cttatcgaga aagaaattac
     1681 cgtcgctcgt gatttgtttg caaaaagaac aaaactgaaa aaacccagac acgctcgact
     1741 tcctgtcatc ctattgattg cagcttccaa tttcgtcaca caacaaggtc ctagcgacgg
     1801 ctcacaggtt ttgtaacaag caatcgaagg ttctggaatg gcgggaaagg gtttagtacc
     1861 acatgctatg atgcccactg tgatctccag agcaaagttc gttcgatcgt actgttactc
     1921 tctctctttc aaacagaatt gtccgaatcg tgtgacaaca acagcctgtt ctcacacact
     1981 cttttcttct aaccaagggg gtggtttagt ttagtagaac ctcgtgaaac ttacatttac
     2041 atatatataa acttgcataa attggtcaat gcaagaaata catatttggt cttttctaat
     2101 tcgtagtttt tcaagttctt agatgctttc tttttctctt ttttacagat catcaaggaa
     2161 gtaattatct actttttaca acaaatataa aacaagatct atggacaaga agtattctat
     2221 cggactggat atcgggacta atagcgtcgg gtgggccgtg atcactgacg agtacaaggt
     2281 gccctctaag aagttcaagg tgctcgggaa caccgaccgg cattccatca agaaaaatct
     2341 gatcggagct ctcctctttg attcagggga aaccgctgaa gcaacccgcc tcaagcggac
     2401 tgctagacgg cggtacacca ggaggaagaa ccggatttgt taccttcaag agatattctc
     2461 caacgaaatg gcaaaggtcg acgacagctt cttccatagg ctggaagaat cattcctcgt
     2521 ggaagaggat aagaagcatg aacggcatcc catcttcggt aatatcgtcg acgaggtggc
     2581 ctatcacgag aaatacccaa ccatctacca tcttcgcaaa aagctggtgg actcaaccga
     2641 caaggcagac ctccggctta tctacctggc cctggcccac atgatcaagt tcagaggcca
     2701 cttcctgatc gagggcgacc tcaatcctga caatagcgat gtggataaac tgttcatcca
     2761 gctggtgcag acttacaacc agctctttga agagaacccc atcaatgcaa gcggagtcga
     2821 tgccaaggcc attctgtcag cccggctgtc aaagagccgc agacttgaga atcttatcgc
     2881 tcagctgccg ggtgaaaaga aaaatggact gttcgggaac ctgattgctc tttcacttgg
     2941 gctgactccc aatttcaagt ctaatttcga cctggcagag gatgccaagc tgcaactgtc
     3001 caaggacacc tatgatgacg atctcgacaa cctcctggcc cagatcggtg accaatacgc
     3061 cgaccttttc cttgctgcta agaatctttc tgacgccatc ctgctgtctg acattctccg
     3121 cgtgaacact gaaatcacca aggcccctct ttcagcttca atgattaagc ggtatgatga
     3181 gcaccaccag gacctgaccc tgcttaaggc actcgtccgg cagcagcttc cggagaagta
     3241 caaggaaatc ttctttgacc agtcaaagaa tggatacgcc ggctacatcg acggaggtgc
     3301 ctcccaagag gaattttata agtttatcaa acctatcctt gagaagatgg acggcaccga
     3361 agagctcctc gtgaaactga atcgggagga tctgctgcgg aagcagcgca ctttcgacaa
     3421 tgggagcatt ccccaccaga tccatcttgg ggagcttcac gccatccttc ggcgccaaga
     3481 ggacttctac ccctttctta aggacaacag ggagaagatt gagaaaattc tcactttccg
     3541 catcccctac tacgtgggac ccctcgccag aggaaatagc cggtttgctt ggatgaccag
     3601 aaagtcagaa gaaactatca ctccctggaa cttcgaagag gtggtggaca agggagccag
     3661 cgctcagtca ttcatcgaac ggatgactaa cttcgataag aacctcccca atgagaaggt
     3721 cctgccgaaa cattccctgc tctacgagta ctttaccgtg tacaacgagc tgaccaaggt
     3781 gaaatatgtc accgaaggga tgaggaagcc cgcattcctg tcaggcgaac aaaagaaggc
     3841 aattgtggac cttctgttca agaccaatag aaaggtgacc gtgaagcagc tgaaggagga
     3901 ctatttcaag aaaattgaat gcttcgactc tgtggagatt agcggggtcg aagatcggtt
     3961 caacgcaagc ctgggtactt accatgatct gcttaagatc atcaaggaca aggattttct
     4021 ggacaatgag gagaacgagg acatccttga ggacattgtc ctgactctca ctctgttcga
     4081 ggaccgggaa atgatcgagg agaggcttaa gacctacgcc catctgttcg acgataaagt
     4141 gatgaagcaa cttaaacgga gaagatatac cggatgggga cgccttagcc gcaaactcat
     4201 caacggaatc cgggacaaac agagcggaaa gaccattctt gatttcctta agagcgacgg
     4261 attcgctaat cgcaacttca tgcaacttat ccatgatgat tccctgacct ttaaggagga
     4321 catccagaag gcccaagtgt ctggacaagg tgactcactg cacgagcata tcgcaaatct
     4381 ggctggttca cccgctatta agaagggtat tctccagacc gtgaaagtcg tggacgagct
     4441 ggtcaaggtg atgggtcgcc ataaaccaga gaacattgtc atcgagatgg ccagggaaaa
     4501 ccagactacc cagaagggac agaagaacag cagggagcgg atgaaaagaa ttgaggaagg
     4561 gattaaggag ctcgggtcac agatccttaa agagcacccg gtggaaaaca cccagcttca
     4621 gaatgagaag ctctatctgt actaccttca aaatggacgc gatatgtatg tggaccaaga
     4681 gcttgatatc aacaggctct cagactacga cgtggaccat atcgtccctc agagcttcct
     4741 caaagacgac tcaattgaca ataaggtgct gactcgctca gacaagaacc ggggaaagtc
     4801 agataacgtg ccctcagagg aagtcgtgaa aaagatgaag aactattggc gccagcttct
     4861 gaacgcaaag ctgatcactc agcggaagtt cgacaatctc actaaggctg agaggggcgg
     4921 actgagcgaa ctggacaaag caggattcat taaacggcaa cttgtggaga ctcggcagat
     4981 tactaaacat gtcgcccaaa tccttgactc acgcatgaat accaagtacg acgaaaacga
     5041 caaacttatc cgcgaggtga aggtgattac cctgaagtcc aagctggtca gcgatttcag
     5101 aaaggacttt caattctaca aagtgcggga gatcaataac tatcatcatg ctcatgacgc
     5161 atatctgaat gccgtggtgg gaaccgccct gatcaagaag tacccaaagc tggaaagcga
     5221 gttcgtgtac ggagactaca aggtctacga cgtgcgcaag atgattgcca aatctgagca
     5281 ggagatcgga aaggccaccg caaagtactt cttctacagc aacatcatga atttcttcaa
     5341 gaccgaaatc acccttgcaa acggtgagat ccggaagagg ccgctcatcg agactaatgg
     5401 ggagactggc gaaatcgtgt gggacaaggg cagagatttc gctaccgtgc gcaaagtgct
     5461 ttctatgcct caagtgaaca tcgtgaagaa aaccgaggtg caaaccggag gcttttctaa
     5521 ggaatcaatc ctccccaagc gcaactccga caagctcatt gcaaggaaga aggattggga
     5581 ccctaagaag tacggcggat tcgattcacc aactgtggct tattctgtcc tggtcgtggc
     5641 taaggtggaa aaaggaaagt ctaagaagct caagagcgtg aaggaactgc tgggtatcac
     5701 cattatggag cgcagctcct tcgagaagaa cccaattgac tttctcgaag ccaaaggtta
     5761 caaggaagtc aagaaggacc ttatcatcaa gctcccaaag tatagcctgt tcgaactgga
     5821 gaatgggcgg aagcggatgc tcgcctccgc tggcgaactt cagaagggta atgagctggc
     5881 tctcccctcc aagtacgtga atttcctcta ccttgcaagc cattacgaga agctgaaggg
     5941 gagccccgag gacaacgagc aaaagcaact gtttgtggag cagcataagc attatctgga
     6001 cgagatcatt gagcagattt ccgagttttc taaacgcgtc attctcgctg atgccaacct
     6061 cgataaagtc cttagcgcat acaataagca cagagacaaa ccaattcggg agcaggctga
     6121 gaatatcatc cacctgttca ccctcaccaa tcttggtgcc cctgccgcat tcaagtactt
     6181 cgacaccacc atcgaccgga aacgctatac ctccaccaaa gaagtgctgg acgccaccct
     6241 catccaccag agcatcaccg gactttacga aactcggatt gacctctcac agctcggagg
     6301 ggatgaggga gctgatccaa aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt
     6361 ataaggatcc taactcgaga ttgaattgaa ttgaaatcga tagatcaatt tttttctttt
     6421 ctctttcccc atcctttacg ctaaaataat agtttatttt attttttgaa tattttttat
     6481 ttatatacgt atatatagac tattatttat cttttaatga ttattaagat ttttattaaa
     6541 aaaaaattcg ctcctctttt aatgccttta tgcagttttt ttttcccatt cgatatttct
     6601 atgtgctgag cagttacaga gatgttacga accactagtg cactgcagta cagtttagct
     6661 tgcctcgtcc ccgccgggtc acccggccag cgacatggag gcccagaata ccctccttga
     6721 cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtaca tttagcccat
     6781 acatccccat gtataatcat ttgcatccat acattttgat ggccgcacgg cgcgaagcaa
     6841 aaattacggc tcctcgctcc agacctgcga gcagggaaac gctcccctca cagacgcgtt
     6901 gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt tgccactgag
     6961 gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc acatcacatc
     7021 cgaacataaa caaaaatggg taaggaaaag actcacgttt cgaggccgcg attaaattcc
     7081 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     7141 gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     7201 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     7261 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     7321 accactgcga tccccggcaa aacagcattc caggtattag aagaatatcc tgattcaggt
     7381 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     7441 aattgtcctt ttaacagcga tcgcgtattt cgtctggctc aggcgcaatc acgaatgaat
     7501 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     7561 gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt
     7621 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     7681 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     7741 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     7801 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaaagtaa ctgacaataa
     7861 aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg tagttgttct
     7921 attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca tctgcccaga
     7981 tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc
     8041 tatactggag tatcacgtgc tataaaaata attataattt aaatttttta atataaatat
     8101 ataaattaaa aatagaaagt aaaaaaagaa attaaagaaa aaatagtttt tgttttccga
     8161 agatgtaaaa gactctaggg ggatcgccaa caaatactac cttttatctt gctcttcctg
     8221 ctctcaggta ttaatgccga attgtttcat cttgtctgtg tagaaaacca cacacgaaaa
     8281 tcctgtgatt ttacatttta cttatcgtta atcgaatgta tatctattta atctgctttt
     8341 cttgtctaat aaatatatat gtaaagtacg ctttttgttg aaatttttta aacctttgtt
     8401 tatttttttt tcttcattcc gtaactcttc taccttcttt atttactttc taaaatccaa
     8461 atacaaaaca taaaaataaa taaacacaga gtaaattccc aaattattcc atcattaaaa
     8521 gatacgaggc gcgtgtaagt tacaggcaag cgatccgtcc cgagcggccg cgattatcaa
     8581 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8641 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8701 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8761 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgggac ccacgctcac
     8821 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     8881 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8941 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9001 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9061 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9121 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9181 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9241 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9301 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9361 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9421 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9481 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9541 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9601 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgtca
     9661 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     9721 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     9781 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     9841 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     9901 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     9961 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    10021 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    10081 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    10141 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    10201 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    10261 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    10321 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    10381 tgttctttcc tgcgttatcc cctgattctg tggataaccg tgcggccgc
//
