LOCUS       Exported                5641 bp ds-DNA     circular SYN 05-APR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 5, 2022 from SnapGene Viewer 5.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             628..2307
                     /codon_start=1
                     /label=L-sDarken
                     /translation="MDVLSPGQGNNTTSPPAPFETGGNTTGISDVTVSYQVITSLLLGT
                     LIFCAVLGNACVVAAIALERSLQNVANYLIGSLAVTDLMVSVLVLPMAALYQVLNKWTL
                     GQVTCDLFIALNVLCCTSSILHLCAIALDRYWAITDPIDYVNKRTPRRAAALISLTWLI
                     GFLISIPPMLGWRTPEDRSDPDACTISKDHGYTIYSTFGAFYIPLLLMLVLYGRIFRAA
                     RFLSSYKNVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHY
                     LSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPI
                     LVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSR
                     YPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKE
                     DGNILGHKLEYNCFDQLRERKTVKTLGIIMGTFILCWLPFFIVALVLPFCESSCHMPTL
                     LGAIINWLGYSNSLLNPVIYAYFNKDFQNAFKKIIKCKFCRQ"
     polyA_signal    2429..2550
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2557..3012)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3039..3143
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3145..3502
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3353..3488
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3537..4331
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4563..4610
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4939..5527
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttatg gatgtgctca gccctggtca gggcaacaac
      661 accacatcac caccggctcc ctttgagacc ggcggcaaca ctactggtat ctccgacgtg
      721 accgtcagct accaagtgat cacctctctg ctgctgggca cgctcatctt ctgcgcggtg
      781 ctgggcaatg cgtgcgtggt ggctgccatc gccttggagc gctccctgca gaacgtggcc
      841 aattatctta ttggctcttt ggcggtcacc gacctcatgg tgtcggtgtt ggtgctgccc
      901 atggccgcgc tgtatcaggt gctcaacaag tggacactgg gccaggtaac ctgcgacctg
      961 ttcatcgccc tcaacgtgct gtgctgcacc tcatccatct tgcacctgtg cgccatcgcg
     1021 ctggacaggt actgggccat cacggacccc atcgactacg tgaacaagag gacgccccgg
     1081 cgcgccgctg cgctcatctc gctcacttgg cttattggct tcctcatctc tatcccgccc
     1141 atgctgggct ggcgcacccc ggaagaccgc tcggaccccg acgcatgcac cattagcaag
     1201 gatcatggct acactatcta ttccaccttt ggagctttct acatcccgct gctgctcatg
     1261 ctggttctct atgggcgcat attccgagct gcgcgcttcc tgagctcata taagaacgtc
     1321 tatatcaagg ccgacaagca gaagaacggc atcaaggcga acttcaagat ccgccacaac
     1381 atcgaggacg gcggcgtgca gctcgcctac cactaccagc agaacacccc catcggcgac
     1441 ggccccgtgc tgctgcccga caaccactac ctgagcgtgc agtccaaact ttcgaaagac
     1501 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1561 ctcggcatgg acgagctgta caagggcggt accggaggga gcatggtgag caagggcgag
     1621 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1681 aagttcagcg tgtccggcga gggtgagggc gatgccacct acggcaagct gaccctgaag
     1741 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     1801 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     1861 tccgccatgc ccgaaggcta catccaggag cgcaccatct tcttcaagga cgacggcaac
     1921 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1981 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactgt
     2041 tttgaccaac tgcgagagag gaagacagtg aagacgctgg gcatcatcat gggcaccttc
     2101 atcctctgct ggctgccctt cttcatcgtg gctcttgttc tgcccttctg cgagagcagc
     2161 tgccacatgc ccaccctgtt gggcgccata atcaattggc tgggctactc caactctctg
     2221 cttaaccccg tcatttacgc atacttcaac aaggactttc aaaacgcgtt taagaagatc
     2281 attaagtgta agttctgccg ccagtgagcg gccgcgactc tagatcataa tcagccatac
     2341 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     2401 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     2461 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     2521 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
     2581 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     2641 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     2701 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     2761 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     2821 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     2881 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     2941 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     3001 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     3061 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     3121 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
     3181 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3241 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3301 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     3361 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     3421 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     3481 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     3541 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     3601 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     3661 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     3721 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     3781 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     3841 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     3901 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     3961 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     4021 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     4081 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     4141 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     4201 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     4261 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     4321 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     4381 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     4441 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     4501 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     4561 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     4621 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     4681 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     4741 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
     4801 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     4861 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     4921 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4981 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     5041 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     5101 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     5161 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5221 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5281 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     5341 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     5401 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     5461 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5521 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     5581 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
     5641 t
//
