LOCUS       498pDestTol2pA2         5441 bp ss-DNA     circular SYN 01-Feb-2012
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    462..513
                     /label="backbone"
     misc_feature    514..1052
                     /label="Tol2 R arm"
     misc_signal     complement(514..1021)
                     /note="Tol2 right arm"
     frag            514..1021
                     /note="659 to 1166 of Kozak_G2AC_in_pBT2"
     primer_bind     919..938
                     /note="cma5"
     primer_bind     1061..1082
                     /note="M13 R"
     misc_recomb     1097..>1102
                     /note="attR4"
     LTR             1102
                     /note="43 LR start"
     frag            join(1103..>1213,<1310..>2053,<2114..2344)
                     /note="1 to 1086 of Untitled [Split]"
     misc_difference 1120..1213
                     /label="QUAS"
     frag            1120..1213
                     /note="688 to 781 of 1078.P5'(QUAS)"
     frag            1337..2053
                     /note="674 to 1390 of 956 pME-EGFP no stop"
     CDS             1337..2053
                     /label="EGFP"
                     /note="; /ApEinfo_fwdcolor=#00ff00;
                     /ApEinfo_revcolor=#00ff00"
     misc_structure  1528..1541
                     /label="EGFP-like chromophore"
                     /note="; /ApEinfo_fwdcolor=#00cc00;
                     /ApEinfo_revcolor=#00cc00"
     misc_feature    2141..2336
                     /note="SV40 late polyA (from pCS2+)"
     frag            2141..2336
                     /note="750 to 945 of 491 p3E-polyA"
     misc_recomb     <2345..2358
                     /note="attR3"
     LTR             2345
                     /note="1727 begin bkbn"
     primer_bind     2367..2382
                     /note="M13 F"
     polyA_signal    2398..2595
                     /note="polyA"
     polyA_signal    2402..2623
                     /note="SV40 late polyA region"
     polyA_signal    complement(2402..2532)
                     /note="SV40 polyadenylation signal"
     misc_signal     2654..3171
                     /note="Tol2 left arm"
     frag            2654..3171
                     /note="3005 to 3522 of Kozak_G2AC_in_pBT2"
     LTR             2654..2988
                     /label="Tol2 L arm"
                     /note="tol2 exon1"
     misc_feature    2654..3171
                     /label="Tol2 L arm"
     LTR             2778..2797
                     /note="cma 103"
     LTR             3152..3170
                     /note="Tol2 L"
     LTR             3152..3170
                     /note="Tol2 L"
     promoter        3256..3275
                     /note="T3"
     misc_feature    3286..3533
                     /note="lac operon sequence"
     misc_feature    complement(3286..3515)
                     /label="M13 REverse"
     primer_bind     3291..3312
                     /note="M13R Complement"
     primer_bind     complement(3296..3317)
                     /note="pUC"
                     /note="; /M13 reverse sequencing primer"
     promoter        complement(3301..3422)
                     /note="Plac"
     primer_bind     complement(3309..3332)
                     /note="Ptac forward sequencing primer (NEB #1260,
                     discontinued)"
     protein_bind    complement(3316..3340)
                     /bound_moiety="LacI"
                     /note="lac operator"
     misc_feature    complement(3515..3578)
                     /citation=[5]
                     /note="L-strand Y effector site"
     rep_origin      3680..3709
                     /note="ColE1-derived plasmid replication origin"
     rep_origin      3695..3731
                     /note="ColE1-derived origin of replication"
     rep_origin      3698..4286
                     /note="pMB1 origin of replication (counterclockwise) (RNAII
                     -35 to RNA"
                     /note="; /DNA switch point)"
     misc_RNA        complement(3698..4250)
                     /note="RNAII transcript (complementary strand)"
     rep_origin      complement(3698..4286)
                     /note="pMB1 origin of replication (clockwise) (RNAII -35 to
                     RNA"
                     /note="; /DNA switch point)"
     misc_RNA        4140..4247
                     /note="RNAI transcript"
     repeat_region   4312..4349
                     /note="corresponds to one of the 38bp repeats found in Tn3
                     (bp 1-38 and complement (4920-4957))"
                     /rpt_type=inverted
     gene            complement(4457..5317)
                     /gene="bla"
     CDS             complement(4457..5317)
                     /codon_start=1
                     /db_xref="GI:455370"
                     /gene="bla"
                     /note="E-286"
                     /product="beta-lactamase"
                     /protein_id="AAB59737.1"
                     /transl_table=11
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     mat_peptide     complement(4460..5248)
                     /gene="bla"
                     /product="beta-lactamase"
     CDS             4460..5317
                     /note="ampR"
     sig_peptide     complement(5249..5317)
                     /gene="bla"
                     /note="Required for secretion to the periplasm"
                     /note="cleaved off to form the mature beta-lactamase
                     protein."
BASE COUNT  1525 A  1288 C  1240 G  1379 T  9 OTHER
ORIGIN
        1 CCACCTAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA
       61 GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA
      121 CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG
      181 ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACGC TGGCTAAGAA
      241 CTCATCAGCC TCCCCGGTCC ATCTACCCAC GTACCAATGC ACCAATTGGC CACAATGACG
      301 GCTACTACAT GGTGCCATTC CTTCCTCTTT ATAGGAATGG AGACTACCTC CTGTCCAACA
      361 AGGCTCTTGG ATACGAGTAC GCCTACCTGT TGGACCCAGG TCATTGCACA ACACCAGAAA
      421 TGCCCTCTGA TCTGCAAAAG ACGTGAATAT CTGTTCAGAC ACCCATATCC ACTCTGTTCC
      481 ACACAGGTCA GAGGTTTGTC CAGGAGTTCT TGACAGAGGT GTAAAAAGTA CTCAAAAATT
      541 TTACTCAAGT GAAAGTACAA GTACTTAGGG AAAATTTTAC TCAATTAAAA GTAAAAGTAT
      601 CTGGCTAGAA TCTTACTTGA GTAAAAGTAA AAAAGTACTC CATTAAAATT GTACTTGAGT
      661 ATTAAGGAAG TAAAAGTAAA AGCAAGAAAG AAAACTAGAG ATTCTTGTTT AAGCTTTTAA
      721 TCTCAAAAAA CATTAAATGA AATGCATACA AGGTTTTATC CTGCTTTAGA ACTGTTTGTA
      781 TTTAATTATC AAACTATAAG ACAGACAATC TAATGCCAGT ACACGCTACT CAAAGTTGTA
      841 AAACCTCAGA TTTAACTTCA GTAGAAGCTG ATTCTCAAAA TTGTTAGTGT CAAGCCTAGC
      901 TCTTTTGGGG CTGAAAAGCA ATCCTGCAGT GCTGAAAAGC CTCTCACAGG CAGCCGATGC
      961 GGGAAGAGGT GTATTAGTCT TGATAGAGAG GCTGCAAATA GCAGGAAACG TGAGCAGAGA
     1021 CTCCCTGGTG TCTGAAACAC AGGCCAGATG GGCCCTCGAG CAGGAAACAG CTATGACCAT
     1081 GATTACGCCA AGCTATCAAC TTTGTATAGA AAAGTTGNNG GGTAATCGCT TATCCTCGGA
     1141 TAAACAATTA TCCTCACGGG TAATCGCTTA TCCGCTCGGG TAATCGCTTA TCCTCGGGTA
     1201 ATCGCTTATC CTTCAAGGGT CGACTCTAGA GGGTATATAA TGGATCCCAT CGCGTCTCAG
     1261 CCTCACTTTG AGCTCCTCCA CACGAATTCC CTCGACCTCG AAGACGCGTN CAAGTTTGTA
     1321 CAAAAAAGCA GGCTNNATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT
     1381 CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA
     1441 GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC
     1501 CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA
     1561 CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA
     1621 GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT
     1681 CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG
     1741 CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC
     1801 CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG
     1861 CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT
     1921 GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA
     1981 GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA
     2041 CGAGCTGTAC AAGGGCGGTG GAAGATCTGG GAATTCAAGG CCTCTCGAGC CTCTAGATTC
     2101 TGCAGCCCTA TAGNACCCAG CTTTCTTGTA CAAAGTGGNN GATCCAGACA TGATAAGATA
     2161 CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA
     2221 AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA
     2281 CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTNCAA
     2341 CTTTATTATA CATAGTTGAT AATTCACTGG CCGTCGTTTT ACGGTACCAT CGATGATGAT
     2401 CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA
     2461 AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC
     2521 AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG
     2581 TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCCT
     2641 CTAGATCAGA TCTGCGAAGA TACGGCCACG GGTGCTCTTG ATCCTGTGGC TGATTTTGGA
     2701 CTGTGCTGCT CGCAGCTGCT GATGAATCAC ATACTTCCTC CATTTTCTTC CACTGATTGA
     2761 CTGTTATAAT TTCCCTAATT TCCAGGTCAA GGTGCTGTGC ATTGTGGTAA TAGATGTGAC
     2821 ATGACGTCAC TTCCAAAGGA CCAATGAACA TGTCTGACCA ATTTCATATA ATGTGAAAAC
     2881 GATTTTCATA GGCAGAATAA ATAACATTTA AATTAAACTG GGCATCAGCG CAATTCAATT
     2941 GGTTTGGTAA TAGCAAGGGA AAATAGAATG AAGTGATCTC CAAAAAATAA GTACTTTTTG
     3001 ACTGTAAATA AAATTGTAAG GAGTAAAAAG TACTTTTTTT TCTAAAAAAA TGTAATTAAG
     3061 TAAAAGTAAA AGTATTGATT TTTAATTGTA CTCAAGTAAA GTAAAAATCC CCAAAAATAA
     3121 TACTTAAGTA CAGTAATCAA GTAAAATTAC TCAAGTACTT TACACCTCTG GTTCTTGACC
     3181 CCCTACCTTC AGCAAGCCCA GCAGATCCAC TAGTTCTAGA GCGGCCGCCA CCGCGGTGGA
     3241 GCTCCAGCTT TTGTTCCCTT TAGTGAGGGT TAATTGCGCG CTTGGCGTAA TCATGGTCAT
     3301 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA
     3361 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC
     3421 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC
     3481 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT
     3541 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC
     3601 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA
     3661 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG
     3721 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA
     3781 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC
     3841 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
     3901 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC
     3961 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
     4021 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT
     4081 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA
     4141 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
     4201 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA
     4261 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG
     4321 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT
     4381 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT
     4441 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC
     4501 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG
     4561 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG
     4621 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT
     4681 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG
     4741 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT
     4801 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA
     4861 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG
     4921 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT
     4981 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA
     5041 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA
     5101 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT
     5161 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT
     5221 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA
     5281 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT
     5341 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA
     5401 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT G
//
