LOCUS       498pDestTol2pA2         6009 bp ss-DNA     circular SYN 01-Feb-2012
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    462..513
                     /label="backbone"
     misc_feature    514..1052
                     /label="Tol2 R arm"
     misc_signal     complement(514..1021)
                     /note="Tol2 right arm"
     frag            514..1021
                     /note="659 to 1166 of Kozak_G2AC_in_pBT2"
     primer_bind     919..938
                     /note="cma5"
     primer_bind     1061..1082
                     /note="M13 R"
     misc_recomb     1097..>1103
                     /note="attR4"
     LTR             1103
                     /note="43 LR start"
     misc_difference 1118..1211
                     /label="QUAS"
     frag            1118..1211
                     /note="688 to 781 of 1078.P5'(QUAS)"
     frag            join(<1212..>2709,<2710..2934)
                     /note="669 to 2500 of Untitled [Split]"
     frag            <1212..1308
                     /note="688 to 878 of 1078.P5'(QUAS)"
     misc_difference 1212..1308
                     /label="carpmpromoter"
     gene            1333..2685
                     /gene="GCaMP7a"
     frag            1333..2682
                     /note="1 to 1350 of Gcamp7a database"
     frag            1333..2682
                     /note="1253 to 2602 of GcamP7a-vector map"
     frag            <1333..>2685
                     /note="1 to 1609 of Untitled [Split]"
     frag            1333..2685
                     /note="669 to 2021 of pME GCaMP7a"
     frag            2710..2905
                     /note="750 to 945 of 491 p3E-polyA"
     misc_feature    2710..2905
                     /note="SV40 late polyA (from pCS2+)"
     primer_bind     2935..2950
                     /note="M13 F"
     polyA_signal    2966..3163
                     /note="polyA"
     polyA_signal    2970..3191
                     /note="SV40 late polyA region"
     polyA_signal    complement(2970..3100)
                     /note="SV40 polyadenylation signal"
     misc_feature    3222..3739
                     /label="Tol2 L arm"
     LTR             3222..3556
                     /label="Tol2 L arm"
                     /note="tol2 exon1"
     frag            3222..3739
                     /note="3005 to 3522 of Kozak_G2AC_in_pBT2"
     misc_signal     3222..3739
                     /note="Tol2 left arm"
     LTR             3346..3365
                     /note="cma 103"
     LTR             3720..3738
                     /note="Tol2 L"
     LTR             3720..3738
                     /note="Tol2 L"
     promoter        3824..3843
                     /note="T3"
     misc_feature    3854..4101
                     /note="lac operon sequence"
     misc_feature    complement(3854..4083)
                     /label="M13 REverse"
     primer_bind     3859..3880
                     /note="M13R Complement"
     primer_bind     complement(3864..3885)
                     /note="pUC"
                     /note="/M13 reverse sequencing primer"
     promoter        complement(3869..3990)
                     /note="Plac"
     primer_bind     complement(3877..3900)
                     /note="Ptac forward sequencing primer (NEB #1260,
                     discontinued)"
     protein_bind    complement(3884..3908)
                     /bound_moiety="LacI"
                     /note="lac operator"
     misc_feature    complement(4083..4146)
                     /citation=[5]
                     /note="L-strand Y effector site"
     rep_origin      4248..4277
                     /note="ColE1-derived plasmid replication origin"
     rep_origin      4263..4299
                     /note="ColE1-derived origin of replication"
     rep_origin      4266..4854
                     /note="pMB1 origin of replication (counterclockwise) (RNAII
                     -35 to RNA"
                     /note="/DNA switch point)"
     misc_RNA        complement(4266..4818)
                     /note="RNAII transcript (complementary strand)"
     rep_origin      complement(4266..4854)
                     /note="pMB1 origin of replication (clockwise) (RNAII -35 to
                     RNA"
                     /note="/DNA switch point)"
     misc_RNA        4708..4815
                     /note="RNAI transcript"
     repeat_region   4880..4917
                     /note="corresponds to one of the 38bp repeats found in Tn3
                     (bp 1-38 and complement (4920-4957))"
                     /rpt_type=inverted
     gene            complement(5025..5885)
                     /gene="bla"
     CDS             complement(5025..5885)
                     /codon_start=1
                     /db_xref="GI:455370"
                     /gene="bla"
                     /note="E-286"
                     /product="beta-lactamase"
                     /protein_id="AAB59737.1"
                     /transl_table=11
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     mat_peptide     complement(5028..5816)
                     /gene="bla"
                     /product="beta-lactamase"
     CDS             5028..5885
                     /note="ampR"
     sig_peptide     complement(5817..5885)
                     /gene="bla"
                     /note="Required for secretion to the periplasm"
                     /note="cleaved off to form the mature beta-lactamase
                     protein."
BASE COUNT  1731 A  1341 C  1416 G  1521 T  0 OTHER
ORIGIN
        1 CCACCTAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA
       61 GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA
      121 CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG
      181 ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACGC TGGCTAAGAA
      241 CTCATCAGCC TCCCCGGTCC ATCTACCCAC GTACCAATGC ACCAATTGGC CACAATGACG
      301 GCTACTACAT GGTGCCATTC CTTCCTCTTT ATAGGAATGG AGACTACCTC CTGTCCAACA
      361 AGGCTCTTGG ATACGAGTAC GCCTACCTGT TGGACCCAGG TCATTGCACA ACACCAGAAA
      421 TGCCCTCTGA TCTGCAAAAG ACGTGAATAT CTGTTCAGAC ACCCATATCC ACTCTGTTCC
      481 ACACAGGTCA GAGGTTTGTC CAGGAGTTCT TGACAGAGGT GTAAAAAGTA CTCAAAAATT
      541 TTACTCAAGT GAAAGTACAA GTACTTAGGG AAAATTTTAC TCAATTAAAA GTAAAAGTAT
      601 CTGGCTAGAA TCTTACTTGA GTAAAAGTAA AAAAGTACTC CATTAAAATT GTACTTGAGT
      661 ATTAAGGAAG TAAAAGTAAA AGCAAGAAAG AAAACTAGAG ATTCTTGTTT AAGCTTTTAA
      721 TCTCAAAAAA CATTAAATGA AATGCATACA AGGTTTTATC CTGCTTTAGA ACTGTTTGTA
      781 TTTAATTATC AAACTATAAG ACAGACAATC TAATGCCAGT ACACGCTACT CAAAGTTGTA
      841 AAACCTCAGA TTTAACTTCA GTAGAAGCTG ATTCTCAAAA TTGTTAGTGT CAAGCCTAGC
      901 TCTTTTGGGG CTGAAAAGCA ATCCTGCAGT GCTGAAAAGC CTCTCACAGG CAGCCGATGC
      961 GGGAAGAGGT GTATTAGTCT TGATAGAGAG GCTGCAAATA GCAGGAAACG TGAGCAGAGA
     1021 CTCCCTGGTG TCTGAAACAC AGGCCAGATG GGCCCTCGAG CAGGAAACAG CTATGACCAT
     1081 GATTACGCCA AGCTATCAAC TTTGTATAGA AAAGTTGGGG TAATCGCTTA TCCTCGGATA
     1141 AACAATTATC CTCACGGGTA ATCGCTTATC CGCTCGGGTA ATCGCTTATC CTCGGGTAAT
     1201 CGCTTATCCT TGCAAGGGTC GACTCTAGAG GGTATATAAT GGATCCCATC GCGTCTCAGC
     1261 CTCACTTTGA GCTCCTCCAC ACGAATTCCC TCGACCTCGA AGACGCGTCA AGTTTGTACA
     1321 AAAAAGCAGG CTATGCGCGG CAGCCACCAC CACCACCACG GCATGGCATC AATGACCGGC
     1381 GGCCAGCAGA TGGGAAGAGA CCTGTATGAC GACGACGACA AAGACCTGGC CACCATGGTG
     1441 GATAGCTCTA GAAGGAAGTG GAATAAAACT GGCCACGCCG TGCGCGCAAT TGGTAGACTG
     1501 TCCTCACTGG AGAACGTCTA CATCAAGGCA GACAAGCAGA AAAACGGCAT CAAGGCTAAC
     1561 TTCAAGATCA GGCACAACAT CGAGGATGGA GGCGTGCAGC TGGCATACCA TTATCAGCAG
     1621 AACACCCCTA TCGGAGACGG CCCTGTGCTG CTGCCAGATA ACCACTACCT GTCAGTCCAG
     1681 AATAAGCTGA GTAAAGACCC AAACGAAAAA CGCGATCATA TGGTGCTGCT GGAGTTCGTC
     1741 ACAGCTGCCG GTATTACCCT GGGAATGGAC GAGCTGTATA AAGGTGGAAC AGGCGGTTCT
     1801 ATGGTGTCCA AGGGAGAGGA ACTGTTCACC GGAGTGGTCC CCATCCTGGT GGAGCTGGAC
     1861 GGCGATGTCA ACGGTCATAA ATTTAGTGTG AGCGGTGAAG GAGAGGGCGA TGCTACTTAC
     1921 GGAAAGCTGA CACTGAAATT CATCTGTACA ACCGGAAAGC TGCCTGTGCC ATGGCCCACA
     1981 CTGGTCACTA CACTGACCTA CGGAGTGCAG TGCTTTAGCA GGTATCCCGA CCACATGAAG
     2041 CAGCATGATT TCTTTAAATC TGCAATGCCT GAAGGCTACA TCCAGGAGAG AACTATTTTC
     2101 TTTAAGGACG ATGGATACTA CAAAACTAGG GCTGAAGTGA AGTTCGAGGG CGACACACTG
     2161 GTGAACCGCA TCGTCCTGAA GGGAATCGAC TTCAAGGAAG ATGGAAACAT CCTGGGCCAC
     2221 AAACTCGAGT ACAATACTAG AGATCAGCTG ACAGAGGAGC AGATTGCTGA GTTCAAAGAG
     2281 GCCTTCAGCC TGTTTGACAA GGATGGCGAC GGTACAATCA CCACTAAGGA GCTGGGCACC
     2341 GTGCTGAGGT CTCTGGGTCA GAACCCAACA GAGGCCGAGC TGCAGGACAT GATCAACGAG
     2401 GTGGATGCAG ACGGAGATGG CACTATTGAT TTCCCCGAGT TTCTGACAAT GATGGCTCGC
     2461 AAGATGAAAT ACACCGACTC AGAGGAAGAG ATCAGGGAGG CCTTCCGCGT GTTTGATAAA
     2521 GACGGTGACG GATACATTAG TGCAGCTGAG CTGAGACATG TGATGACCAA TCTGGGAGAG
     2581 AAGCTGACTG ACGAGGAAGT GGATGAGATG ATCAGGGAGG CAGATATTGA CGGCGATGGT
     2641 CAGGTGGACT ATGAGGAGTT CGTGCAGATG ATGACCGCTA AGTAGACCCA GCTTTCTTGT
     2701 ACAAAGTGGG ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA
     2761 ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC
     2821 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT
     2881 CAGGGGGAGG TGTGGGAGGT TTTTTCAACT TTATTATACA TAGTTGATAA TTCACTGGCC
     2941 GTCGTTTTAC GGTACCATCG ATGATGATCC AGACATGATA AGATACATTG ATGAGTTTGG
     3001 ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT
     3061 TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA
     3121 TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA
     3181 CAAATGTGGT ATGGCTGATT ATGATCCTCT AGATCAGATC TGCGAAGATA CGGCCACGGG
     3241 TGCTCTTGAT CCTGTGGCTG ATTTTGGACT GTGCTGCTCG CAGCTGCTGA TGAATCACAT
     3301 ACTTCCTCCA TTTTCTTCCA CTGATTGACT GTTATAATTT CCCTAATTTC CAGGTCAAGG
     3361 TGCTGTGCAT TGTGGTAATA GATGTGACAT GACGTCACTT CCAAAGGACC AATGAACATG
     3421 TCTGACCAAT TTCATATAAT GTGAAAACGA TTTTCATAGG CAGAATAAAT AACATTTAAA
     3481 TTAAACTGGG CATCAGCGCA ATTCAATTGG TTTGGTAATA GCAAGGGAAA ATAGAATGAA
     3541 GTGATCTCCA AAAAATAAGT ACTTTTTGAC TGTAAATAAA ATTGTAAGGA GTAAAAAGTA
     3601 CTTTTTTTTC TAAAAAAATG TAATTAAGTA AAAGTAAAAG TATTGATTTT TAATTGTACT
     3661 CAAGTAAAGT AAAAATCCCC AAAAATAATA CTTAAGTACA GTAATCAAGT AAAATTACTC
     3721 AAGTACTTTA CACCTCTGGT TCTTGACCCC CTACCTTCAG CAAGCCCAGC AGATCCACTA
     3781 GTTCTAGAGC GGCCGCCACC GCGGTGGAGC TCCAGCTTTT GTTCCCTTTA GTGAGGGTTA
     3841 ATTGCGCGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC
     3901 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA
     3961 GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG
     4021 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG
     4081 CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG
     4141 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA
     4201 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG
     4261 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG
     4321 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC
     4381 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG
     4441 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT
     4501 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC
     4561 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC
     4621 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG
     4681 TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA
     4741 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC
     4801 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT
     4861 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     4921 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     4981 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     5041 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     5101 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     5161 CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     5221 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     5281 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     5341 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     5401 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     5461 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     5521 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     5581 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     5641 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     5701 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     5761 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     5821 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     5881 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     5941 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC
     6001 CGAAAAGTG
//
