LOCUS       CBS-1708_pBAD33-T7promo 7406 bp ds-DNA     circular     15-NOV-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      9..337
                     /label="p15A_ori"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    500..516
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     RBS             517..522
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    531..3890
                     /label="PgiCas13b"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             531..3890
                     /label="Translation 531-3890"
                     /translation="MTEQNEKPYNGTYYTLEDKHFWAAFLNLARHNAYITLAHIDRQLAYSKADITNDEDILFFKGQWKNLDNDLERKARLRSLILKHFSFLEGAAYGKKLFESQSSGNKSSKKKELSKKEKEELQANALSLDNLKSILFDFLQKLKDFRNYYSHYRHPESSELPLFDGNMLQRLYNVFDVSVQRVKRDHEHNDKVDPHRHFNHLVRKGKKDKYGNNDNPFFKHHFVDREGTVTEAGLLFFVSLFLEKRDAIWMQKKIRGFKGGTEAYQQMTNEVFCRSRISLPKLKLESLRTDDWMLLDMLNELVRCPKSLYDRLREEDRARFRVPVDILSDEDDTDGTEEDPFKNTLVRHQDRFPYFALRYFDLKKVFTSLRFHIDLGTYHFAIYKKNIGEQPEDRHLTRNLYGFGRIQDFAEEHRPEEWKRLVRDLDYFETGDKPYITQTTPHYHIEKGKIGLRFVPEGQHLWPSPEVGATRTGRSKYAQDKRLTAEAFLSVHELMPMMFYYFLLREKYSEEVSAEKVQGRIKRVIEDVYAVYDAFARDEINTRDELDACLADKGIRRGHLPRQMIAILSQEHKDMEEKVRKKLQEMIADTDHRLDMLDRQTDRKIRIGRKNAGLPKSGVVADWLVRDMMRFQPVAKDTSGKPLNNSKANSTEYRMLQRALALFGGEKERLTPYFRQMNLTGGNNPHPFLHETRWESHTNILSFYRSYLEARKAFLQSIGRSDRVENHRFLLLKEPKTDRQTLVAGWKGEFHLPRGIFTEAVRDCLIEMGYDEVGSYKEVGFMAKAVPLYFERASKDRVQPFYDYPFNVGNSLKPKKGRFLSKEKRAEEWESGKERFRLAKLKKEILEAKEHPYHDFKSWQKFERELRLVKNQDIITWMMCRDLMEENKVEGLDTGTLYLKDIRTDVQEQGSLNVLNRVKPMRLPVVVYRADSRGHVHKEQAPLATVYIEERDTKLLKQGNFKSFVKDRRLNGLFSFVDTGALAMEQYPISKLRVEYELAKYQTARVCAFEQTLELEESLLTRYPHLPDKNFRKMLESWSDPLLDKWPDLHGNVRLLIAVRNAFSHNQYPMYDETLFSSIRKYDPSSPDAIEERMGLNIAHRLSEEVKQAKEMVERIIQA*"
     misc_feature    3972..3990
                     /label="primer KM113"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3979..4136
                     /label="rrnB_term"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4102..4145
                     /label="rrnB_T1 term"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(4546..4830)
                     /label="Ampicillin"
                     /ApEinfo_revcolor="#ff1d00"
                     /ApEinfo_fwdcolor="#ff1d00"
     misc_feature    4889..5188
                     /label="f1 Origin"
                     /ApEinfo_revcolor="#0f770d"
                     /ApEinfo_fwdcolor="#0f770d"
     CDS             complement(5886..6542)
                     /label="CAT/CmR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      6904..8
                     /label="p15A_ori"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
ORIGIN
        1 atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag
       61 tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga
      121 ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga
      181 ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga
      241 aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa
      301 acgatctcaa gaagatcatc ttattaatca gataaaatat ttctaggctc atgagcccga
      361 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
      421 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctgc tcatgtttga
      481 cagcttatca tcgatgcatt aatacgactc actataAGGA GGTCTTTATC ATGACGGAGC
      541 AGAACGAGAA ACCCTATAAC GGCACATATT ACACATTAGA AGACAAACAT TTCTGGGCAG
      601 CATTTTTGAA CCTTGCTCGT CATAACGCCT ACATCACTTT GGCCCACATT GATCGCCAGC
      661 TTGCCTACTC CAAGGCGGAT ATCACGAACG ATGAGGACAT CCTGTTCTTC AAAGGCCAAT
      721 GGAAGAATTT GGACAATGAT CTTGAGCGTA AAGCGCGCCT GCGTAGCTTG ATCTTGAAGC
      781 ACTTTTCCTT TCTTGAAGGG GCTGCCTACG GGAAGAAGTT ATTCGAATCT CAGTCAAGCG
      841 GGAACAAGTC ATCTAAAAAA AAGGAACTGT CCAAAAAGGA AAAAGAAGAG TTGCAAGCCA
      901 ATGCGTTGAG CCTGGACAAC CTTAAAAGTA TTTTATTTGA CTTTCTGCAG AAGTTAAAGG
      961 ATTTTCGTAA CTACTATAGC CACTATCGTC ACCCGGAAAG CTCGGAGCTT CCCTTATTTG
     1021 ATGGCAATAT GTTGCAACGC CTGTATAACG TATTTGATGT GTCGGTCCAG CGCGTTAAGC
     1081 GTGACCACGA GCACAACGAC AAGGTTGATC CTCATCGCCA TTTTAATCAC TTAGTTCGCA
     1141 AAGGCAAAAA GGACAAATAC GGTAATAATG ATAATCCATT CTTCAAACAT CACTTCGTTG
     1201 ACCGCGAGGG GACAGTTACA GAGGCAGGTC TTCTGTTCTT TGTCTCATTA TTCCTTGAGA
     1261 AACGTGACGC AATTTGGATG CAAAAGAAAA TCCGTGGGTT TAAGGGAGGT ACGGAAGCCT
     1321 ACCAGCAAAT GACTAACGAG GTTTTCTGCC GTTCGCGCAT TAGCCTTCCT AAACTTAAAT
     1381 TAGAATCCTT GCGTACGGAT GACTGGATGC TGCTGGACAT GCTTAACGAA TTAGTACGTT
     1441 GTCCTAAGAG TCTGTATGAC CGTCTTCGTG AAGAAGACCG CGCTCGCTTT CGTGTACCTG
     1501 TAGACATTCT GAGCGACGAA GATGATACAG ACGGCACGGA GGAAGATCCC TTTAAAAATA
     1561 CATTAGTACG TCACCAGGAT CGTTTCCCCT ACTTTGCATT ACGCTACTTT GATTTGAAAA
     1621 AGGTGTTCAC CTCTTTACGT TTCCACATCG ATCTTGGTAC CTACCATTTT GCGATCTACA
     1681 AAAAGAATAT TGGGGAGCAA CCCGAGGATC GTCATTTGAC CCGTAATTTG TACGGGTTTG
     1741 GTCGTATTCA GGATTTTGCA GAAGAGCACC GTCCGGAGGA GTGGAAACGC TTGGTGCGCG
     1801 ACTTGGACTA CTTTGAAACC GGTGATAAGC CCTATATTAC GCAGACTACT CCACACTACC
     1861 ACATTGAGAA AGGTAAAATT GGGTTACGTT TTGTGCCAGA GGGTCAACAT TTATGGCCTA
     1921 GCCCTGAGGT TGGGGCCACC CGTACGGGCC GCTCTAAGTA TGCGCAAGAC AAACGCCTTA
     1981 CTGCGGAGGC ATTTTTGTCT GTTCACGAGT TAATGCCCAT GATGTTCTAT TACTTCCTGT
     2041 TACGTGAGAA ATATTCTGAA GAAGTCTCCG CGGAGAAAGT GCAAGGGCGT ATTAAACGCG
     2101 TCATCGAGGA CGTCTATGCT GTGTACGACG CCTTCGCACG CGACGAGATT AACACCCGCG
     2161 ATGAGCTTGA CGCCTGTCTG GCTGACAAGG GGATCCGTCG CGGACACCTG CCCCGTCAGA
     2221 TGATTGCAAT CTTGTCGCAG GAACATAAGG ACATGGAAGA GAAGGTACGT AAGAAACTTC
     2281 AGGAAATGAT CGCTGACACG GATCACCGTT TGGACATGCT GGACCGCCAA ACAGATCGCA
     2341 AAATTCGCAT TGGCCGTAAA AATGCAGGAC TTCCTAAGTC CGGGGTCGTA GCAGATTGGC
     2401 TTGTGCGTGA CATGATGCGT TTTCAGCCCG TGGCGAAAGA TACTAGCGGG AAACCACTGA
     2461 ACAACAGTAA GGCGAACTCC ACTGAATATC GCATGTTGCA GCGCGCCTTG GCTCTGTTTG
     2521 GGGGCGAGAA GGAGCGTCTT ACGCCGTACT TCCGTCAAAT GAATTTAACG GGAGGTAATA
     2581 ACCCGCATCC CTTCCTTCAT GAAACACGCT GGGAAAGCCA CACCAATATC TTGTCATTTT
     2641 ATCGCTCATA TTTAGAAGCC CGTAAAGCGT TCCTTCAGTC GATTGGGCGC TCCGATCGTG
     2701 TTGAGAATCA CCGCTTCCTT TTACTTAAGG AGCCCAAAAC AGACCGTCAA ACATTGGTTG
     2761 CAGGATGGAA GGGTGAGTTC CACTTGCCTC GTGGGATCTT CACCGAGGCT GTCCGCGACT
     2821 GTCTTATCGA AATGGGCTAC GATGAGGTTG GTTCGTATAA GGAAGTCGGG TTCATGGCAA
     2881 AGGCTGTACC ACTGTACTTT GAGCGTGCTT CCAAAGACCG TGTACAACCC TTTTATGATT
     2941 ATCCGTTTAA CGTAGGAAAC TCTTTGAAAC CAAAGAAAGG ACGTTTCCTG TCGAAAGAAA
     3001 AGCGTGCAGA GGAGTGGGAA AGTGGGAAAG AGCGTTTTCG CTTAGCAAAG TTGAAGAAAG
     3061 AAATCCTGGA GGCCAAGGAG CATCCATATC ACGATTTCAA ATCTTGGCAA AAATTCGAGC
     3121 GCGAGCTTCG CTTAGTAAAG AATCAGGATA TCATTACTTG GATGATGTGT CGTGATTTGA
     3181 TGGAAGAGAA TAAGGTTGAG GGCTTAGATA CAGGTACCCT GTATTTGAAG GACATTCGCA
     3241 CGGACGTGCA GGAACAGGGG AGCCTGAATG TTCTTAACCG TGTGAAGCCG ATGCGCCTTC
     3301 CTGTTGTCGT ATATCGTGCC GACAGTCGCG GTCACGTCCA TAAAGAACAG GCCCCTTTGG
     3361 CAACTGTGTA CATCGAAGAA CGCGATACTA AACTTTTAAA ACAAGGCAAC TTTAAGAGTT
     3421 TCGTTAAGGA CCGCCGCCTG AACGGATTGT TCTCTTTCGT AGATACAGGT GCTCTTGCCA
     3481 TGGAACAGTA TCCGATTAGT AAACTGCGCG TGGAGTATGA GCTTGCCAAA TATCAGACTG
     3541 CTCGCGTTTG CGCTTTCGAA CAAACACTGG AGTTAGAAGA GTCGTTACTT ACTCGTTACC
     3601 CACACCTGCC TGACAAGAAC TTCCGCAAAA TGCTGGAGTC GTGGTCGGAT CCGCTGTTAG
     3661 ATAAATGGCC CGACCTTCAC GGGAATGTCC GCTTATTAAT TGCGGTTCGC AATGCTTTCT
     3721 CACACAATCA ATATCCAATG TATGATGAGA CCTTGTTTTC ATCTATCCGT AAATACGACC
     3781 CTAGTAGCCC AGACGCCATC GAAGAACGCA TGGGCTTAAA CATCGCACAT CGTTTATCCG
     3841 AAGAAGTCAA GCAGGCAAAA GAGATGGTTG AACGTATTAT TCAAGCTTAA aagcttggct
     3901 gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg
     3961 gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc
     4021 cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag
     4081 tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     4141 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
     4201 ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc
     4261 aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact
     4321 cttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4381 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4441 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4501 gaaagtaaaa gatgctgaag atcagttggg tgcagcaaac tattaactgg cgaactactt
     4561 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4621 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4681 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     4741 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     4801 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     4861 tagattgatt tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4921 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4981 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5041 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     5101 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5161 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc
     5221 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5281 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttaaa aggatctagg
     5341 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5401 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5461 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5521 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5581 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5641 catacctcgc tctgctaatc ctgttaccag tcaggcattt gagaagcaca cggtcacact
     5701 gcttccggta gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc
     5761 ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt catccgctta
     5821 ttatcactta ttcaggcgta gcaaccaggc gtttaagggc accaataact gccttaaaaa
     5881 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
     5941 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
     6001 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
     6061 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata
     6121 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     6181 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     6241 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     6301 tcaccgtctt tcattgccat acggaattcc ggatgagcat tcatcaggcg ggcaagaatg
     6361 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     6421 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     6481 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     6541 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     6601 cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc
     6661 caaaagttgg cccagggctt cccggtatca acagggacac caggatttat ttattctgcg
     6721 aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga tgctgccaac
     6781 ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag
     6841 ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca
     6901 tcagcgctag cggagtgtat actggcttac tatgttggca ctgatgaggg tgtcagtgaa
     6961 gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg tcagcagaat atgtgataca
     7021 ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag
     7081 cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca
     7141 gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca
     7201 tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca
     7261 ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg
     7321 tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt
     7381 aggcagttcg ctccaagctg gactgt
//