LOCUS       CBS-3816_lac-PbuCas13b- 7816 bp ds-DNA     circular     26-NOV-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(220..322)
                     /label="cat promoter"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     rep_origin      complement(848..1393)
                     /label="p15A ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        1683..1712
                     /label="Lac promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     RBS             1713..1718
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     gene            1727..5110
                     /label="pbcas13b"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             1727..5110
                     /label="Translation 1727-5110"
     promoter        5112..5146
                     /label="J23119 promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    5152..5187
                     /label="DR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5188..5217
                     /label="Spacer-T-deGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5218..5253
                     /label="DR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     gene            complement(join(7376..7816,1..219))
                     /label="CmR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 GAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT
       61 GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT
      121 ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA
      181 TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA
      241 AAATCTCGAT AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT
      301 GGAACCTCTT ACGTGCCGAT CAACGTCTCA TTTTCGCCAA AAGTTGGCCC AGGGCTTCCC
      361 GGTATCAACA GGGACACCAG GATTTATTTA TTCTGCGAAG TGATCTTCCG TCACAGGTAT
      421 TTATTCGGCG CAAAGTGCGT CGGGTGATGC TGCCAACTTA CTGATTTAGT GTATGATGGT
      481 GTTTTTGAGG TGCTCCAGTG GCTTCTGTTT CTATCAGCTG TCCCTCCTGT TCAGCTACTG
      541 ACGGGGTGGT GCGTAACGGC AAAAGCACCG CCGGACATCA GCGCTAGCGG AGTGTATACT
      601 GGCTTACTAT GTTGGCACTG ATGAGGGTGT CAGTGAAGTG CTTCATGTGG CAGGAGAAAA
      661 AAGGCTGCAC CGGTGCGTCA GCAGAATATG TGATACAGGA TATATTCCGC TTCCTCGCTC
      721 ACTGACTCGC TACGCTCGGT CGTTCGACTG CGGCGAGCGG AAATGGCTTA CGAACGGGGC
      781 GGAGATTTCC TGGAAGATGC CAGGAAGATA CTTAACAGGG AAGTGAGAGG GCCGCGGCAA
      841 AGCCGTTTTT CCATAGGCTC CGCCCCCCTG ACAAGCATCA CGAAATCTGA CGCTCAAATC
      901 AGTGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGCGGCTCCC
      961 TCGTGCGCTC TCCTGTTCCT GCCTTTCGGT TTACCGGTGT CATTCCGCTG TTATGGCCGC
     1021 GTTTGTCTCA TTCCACGCCT GACACTCAGT TCCGGGTAGG CAGTTCGCTC CAAGCTGGAC
     1081 TGTATGCACG AACCCCCCGT TCAGTCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
     1141 GAGTCCAACC CGGAAAGACA TGCAAAAGCA CCACTGGCAG CAGCCACTGG TAATTGATTT
     1201 AGAGGAGTTA GTCTTGAAGT CATGCGCCGG TTAAGGCTAA ACTGAAAGGA CAAGTTTTGG
     1261 TGACTGCGCT CCTCCAAGCC AGTTACCTCG GTTCAAAGAG TTGGTAGCTC AGAGAACCTT
     1321 CGAAAAACCG CCCTGCAAGG CGGTTTTTTC GTTTTCAGAG CAAGAGATTA CGCGCAGACC
     1381 AAAACGATCT CAAGAAGATC ATCTTATTAA TCAGATAAAA TATTTCTAGA TTTCAGTGCA
     1441 ATTTATCTCT TCAAATGTAG CACCTGAAGT CAGCCCCATA CGATATAAGT TGTAATTCTC
     1501 ATGTTTGACA GCTTATCATC GATAAGCTTT AATGCGGTAG TTTATCACAG TTAAATTGCT
     1561 AACGCAGTCA GGCACCGTGT ATGAAATCTA ACAATGCGCT CATCGTCATC CTCGGCACCG
     1621 TCACCCTGGA TGCTGTAGGC ATAGGCTTGG TTATGCCGGT ACTGCCGGGC CTCTTGCGGG
     1681 ATTTTACACT TTATGCTTCC GGCTCGTATG TTAGGAGGTC TTTATCATGC AAAAACAAGA
     1741 TAAATTATTT GTAGATAGAA AGAAAAATGC TATATTTGCC TTTCCTAAAT ATATAACAAT
     1801 AATGGAAAAC AAAGAGAAAC CTGAACCAAT TTATTATGAA TTGACAGATA AGCATTTCTG
     1861 GGCTGCCTTT CTTAACTTGG CGCGCCACAA TGTCTATACC ACCATTAATC ACATCAACAG
     1921 ACGTTTGGAA ATAGCAGAGC TAAAAGACGA TGGATATATG ATGGGCATTA AAGGCAGCTG
     1981 GAACGAGCAA GCGAAAAAAC TCGACAAAAA AGTTCGGCTG AGAGACTTGA TCATGAAACA
     2041 CTTTCCTTTT CTGGAAGCTG CAGCTTATGA AATGACAAAC TCTAAAAGTC CCAACAATAA
     2101 GGAGCAACGG GAAAAGGAAC AATCCGAAGC CCTATCACTT AACAACTTGA AGAATGTTCT
     2161 ATTCATTTTC TTGGAAAAAC TTCAGGTTCT CAGGAACTAC TACTCCCATT ACAAGTATTC
     2221 TGAAGAATCC CCAAAACCGA TATTTGAAAC AAGCCTTTTG AAAAACATGT ATAAGGTTTT
     2281 CGATGCCAAT GTGCGGTTAG TAAAGAGAGA CTATATGCAT CATGAGAATA TAGACATGCA
     2341 AAGAGATTTT ACACATCTCA ACAGGAAAAA ACAAGTGGGA CGAACAAAAA ACATCATAGA
     2401 CAGTCCTAAC TTTCATTACC ATTTTGCAGA TAAAGAAGGC AATATGACCA TAGCAGGCTT
     2461 ACTGTTCTTC GTCTCTTTAT TTCTTGATAA AAAAGACGCT ATTTGGATGC AGAAAAAGCT
     2521 AAAGGGATTT AAAGATGGCA GAAACTTGCG TGAGCAAATG ACAAACGAAG TATTTTGCAG
     2581 AAGCCGTATA TCTTTGCCTA AGCTAAAATT AGAGAATGTA CAGACAAAAG ACTGGATGCA
     2641 ACTTGATATG CTTAACGAAT TGGTTCGCTG TCCCAAATCG CTGTATGAAC GGTTACGAGA
     2701 AAAAGATAGG GAAAGTTTTA AAGTACCTTT CGATATTTTC TCTGACGACT ATAATGCTGA
     2761 AGAAGAACCA TTTAAGAACA CGCTTGTAAG GCATCAAGAC CGCTTTCCAT ATTTTGTCTT
     2821 GAGATATTTC GATTTAAATG AGATATTCGA ACAACTCCGC TTCCAAATAG ATTTAGGTAC
     2881 CTATCATTTC TCTATCTATA ACAAACGTAT CGGTGACGAA GATGAAGTTC GCCATCTCAC
     2941 ACATCATCTA TATGGCTTTG CGCGCATTCA GGACTTTGCA CCACAAAACC AACCAGAAGA
     3001 ATGGAGAAAA TTAGTGAAAG ACTTAGATCA TTTTGAGACA TCGCAAGAGC CTTACATATC
     3061 TAAGACTGCT CCACATTACC ATTTAGAGAA TGAAAAGATA GGCATTAAGT TTTGCAGTGC
     3121 CCACAACAAC CTCTTTCCAT CTCTTCAGAC CGACAAAACA TGCAATGGAA GAAGTAAATT
     3181 TAATCTCGGA ACACAATTTA CAGCAGAAGC ATTTTTAAGT GTCCACGAAT TGTTGCCGAT
     3241 GATGTTTTAT TATCTGTTAT TGACCAAGGA TTACTCCAGA AAAGAAAGCG CAGACAAAGT
     3301 AGAGGGAATT ATAAGAAAAG AAATAAGCAA CATATACGCT ATCTACGATG CTTTTGCCAA
     3361 TAATGAGATA AATAGCATTG CAGACTTAAC CCGTCGGCTT CAAAATACAA ATATACTACA
     3421 AGGCCATTTG CCAAAACAAA TGATTTCCAT ATTGAAAGGC AGACAAAAAG ATATGGGAAA
     3481 AGAGGCTGAG CGCAAAATTG GGGAAATGAT AGACGACACC CAAAGAAGAC TTGACTTGCT
     3541 CTGCAAGCAG ACAAATCAGA AGATAAGGAT TGGCAAAAGG AATGCCGGTC TGCTAAAATC
     3601 GGGAAAAATT GCAGATTGGT TGGTAAATGA CATGATGCGC TTCCAACCTG TTCAAAAAGA
     3661 CCAAAATAAC ATTCCAATAA ACAATAGCAA GGCTAACAGC ACCGAATATC GAATGCTTCA
     3721 GCGTGCGCTT GCCCTTTTCG GCAGTGAGAA CTTCCGCCTT AAGGCATATT TTAATCAAAT
     3781 GAATCTTGTG GGGAATGACA ATCCGCATCC TTTCCTTGCA GAAACACAAT GGGAACATCA
     3841 AACTAACATT CTGTCTTTCT ACCGAAACTA TTTGGAAGCG AGGAAAAAGT ATCTTAAGGG
     3901 CTTAAAACCA CAAAACTGGA AACAATATCA GCATTTTCTC ATACTTAAAG TACAGAAGAC
     3961 CAACCGCAAC ACATTAGTTA CAGGATGGAA AAACAGTTTC AATCTCCCCC GTGGAATATT
     4021 TACCCAACCA ATAAGGGAAT GGTTTGAGAA ACATAATAAT TCAAAAAGAA TATACGACCA
     4081 AATACTATCT TTTGACCGAG TAGGGTTTGT TGCAAAAGCT ATTCCCTTAT ACTTTGCAGA
     4141 AGAATATAAA GACAATGTAC AGCCTTTCTA CGATTATCCG TTCAATATCG GCAACAGACT
     4201 TAAACCAAAA AAAAGGCAAT TCTTAGATAA AAAAGAGCGT GTCGAATTGT GGCAAAAGAA
     4261 TAAAGAGCTA TTCAAAAATT ATCCGTCTGA AAAGAAAAAG ACAGACTTAG CTTATCTTGA
     4321 TTTTTTATCA TGGAAAAAGT TCGAACGCGA ACTGCGACTT ATCAAGAATC AGGATATCGT
     4381 CACATGGTTG ATGTTCAAAG AACTATTCAA TATGGCTACT GTGGAAGGAT TGAAGATTGG
     4441 AGAAATACAT CTGAGGGACA TCGATACAAA CACGGCAAAT GAGGAAAGCA ACAATATTCT
     4501 AAATAGAATC ATGCCCATGA AGTTGCCTGT TAAAACATAC GAAACAGACA ATAAAGGAAA
     4561 TATATTAAAG GAAAGACCTT TAGCAACCTT TTATATCGAA GAAACGGAAA CCAAAGTCCT
     4621 GAAACAGGGC AATTTCAAAG CATTGGTAAA AGACCGAAGA CTCAATGGGC TATTCTCATT
     4681 TGCAGAAACT ACAGACTTGA ACTTAGAGGA ACATCCTATC AGCAAATTGA GTGTAGACCT
     4741 TGAGCTTATA AAATATCAGA CTACAAGGAT TAGTATCTTT GAGATGACAT TAGGACTGGA
     4801 AAAGAAACTC ATAGACAAAT ATTCCACACT ACCTACAGAT AGCTTCAGAA ATATGCTTGA
     4861 AAGGTGGTTG CAGTGCAAAG CTAATAGACC GGAATTAAAG AATTACGTTA ATAGTTTGAT
     4921 TGCAGTGAGA AATGCTTTTT CCCACAACCA GTATCCCATG TATGATGCCA CACTCTTTGC
     4981 TGAAGTTAAA AAATTTACTC TTTTCCCTTC TGTCGATACA AAAAAGATTG AGCTAAATAT
     5041 AGCGCCACAG TTACTGGAAA TAGTAGGAAA AGCCATAAAA GAAATTGAAA AATCAGAAAA
     5101 TAAAAACTAA ATTGACAGCT AGCTCAGTCC TAGGTATAAT GCTAGCGCTG AGTTGCATCT
     5161 GCCTTCTTTT TGAAAGGTAA AAACAACCGT CGCCGTCCAG CTCGACCAGG ATGGGAAGTT
     5221 GCATCTGCCT TCTTTTTGAA AGGTAAAAAC AACATCGTCC ATTCCGACAG CATCGCCAGT
     5281 CACTATGGCG TGCTGCTAGC GCTATATGCG TTGATGCAAT TTCTATGCGC ACCCGTTCTC
     5341 GGAGCACTGT CCGACCGCTT TGGCCGCCGC CCAGTCCTGC TCGCTTCGCT ACTTGGAGCC
     5401 ACTATCGACT ACGCGATCAT GGCGACCACA CCCGTCCTGT GGATCCTCTA CGCCGGACGC
     5461 ATCGTGGCCG GCATCACCGG CGCCACAGGT GCGGTTGCTG GCGCCTATAT CGCCGACATC
     5521 ACCGATGGGG AAGATCGGGC TCGCCACTTC GGGCTCATGA GCGCTTGTTT CGGCGTGGGT
     5581 ATGGTGGCAG GCCCCGTGGC CGGGGGACTG TTGGGCGCCA TCTCCTTGCA TGCACCATTC
     5641 CTTGCGGCGG CGGTGCTCAA CGGCCTCAAC CTACTACTGG GCTGCTTCCT AATGCAGGAG
     5701 TCGCATAAGG GAGAGCGTCG ACCGATGCCC TTGAGAGCCT TCAACCCAGT CAGCTCCTTC
     5761 CGGTGGGCGC GGGGCATGAC TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA
     5821 CTCGTAGGAC AGGTGCCGGC AGCGCTCTGG GTCATTTTCG GCGAGGACCG CTTTCGCTGG
     5881 AGCGCGACGA TGATCGGCCT GTCGCTTGCG GTATTCGGAA TCTTGCACGC CCTCGCTCAA
     5941 GCCTTCGTCA CTGGTCCCGC CACCAAACGT TTCGGCGAGA AGCAGGCCAT TATCGCCGGC
     6001 ATGGCGGCCG ACGCGCTGGG CTACGTCTTG CTGGCGTTCG CGACGCGAGG CTGGATGGCC
     6061 TTCCCCATTA TGATTCTTCT CGCTTCCGGC GGCATCGGGA TGCCCGCGTT GCAGGCCATG
     6121 CTGTCCAGGC AGGTAGATGA CGACCATCAG GGACAGCTTC AAGGATCGCT CGCGGCTCTT
     6181 ACCAGCCTAA CTTCGATCAT TGGACCGCTG ATCGTCACGG CGATTTATGC CGCCTCGGCG
     6241 AGCACATGGA ACGGGTTGGC ATGGATTGTA GGCGCCGCCC TATACCTTGT CTGCCTCCCC
     6301 GCGTTGCGTC GCGGTGCATG GAGCCGGGCC ACCTCGACCT GAATGGAAGC CGGCGGCACC
     6361 TCGCTAACGG ATTCACCACT CCAAGAATTG GAGCCAATCA ATTCTTGCGG AGAACTGTGA
     6421 ATGCGCAAAC CAACCCTTGG CAGAACATAT CCATCGCGTC CGCCATCTCC AGCAGCCGCA
     6481 CGCGGCGCAT CTCGGGCAGC GTTGGGTCCT GGCCACGGGT GCGCATGATC GTGCTCCTGT
     6541 CGTTGAGGAC CCGGCTAGGC TGGCGGGGTT GCCTTACTGG TTAGCAGAAT GAATCACCGA
     6601 TACGCGAGCG AACGTGAAGC GACTGCTGCT GCAAAACGTC TGCGACCTGA GCAACAACAT
     6661 GAATGGTCTT CGGTTTCCGT GTTTCGTAAA GTCTGGAAAC GCGGAAGTCC CCTACGTGCT
     6721 GCTGAAGTTG CCCGCAACAG AGAGTGGAAC CAACCGGTGA TACCACGATA CTATGACTGA
     6781 GAGTCAACGC CATGAGCGGC CTCATTTCTT ATTCTGAGTT ACAACAGTCC GCACCGCTGT
     6841 CCGGTAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT CGCCGCACTT ATGACTGTCT
     6901 TCTTTATCAT GCAACTCGTA GGACAGGTGC CGGCAGCGCC CAACAGTCCC CCGGCCACGG
     6961 GGCCTGCCAC CATACCCACG CCGAAACAAG CGCCCTGCAC CATTATGTTC CGGATCTGCA
     7021 TCGCAGGATG CTGCTGGCTA CCCTGTGGAA CACCTACATC TGTATTAACG AAGCGCTAAC
     7081 CGTTTTTATC AGGCTCTGGG AGGCAGAATA AATGATCATA TCGTCAATTA TTACCTCCAC
     7141 GGGGAGAGCC TGAGCAAACT GGCCTCAGGC ATTTGAGAAG CACACGGTCA CACTGCTTCC
     7201 GGTAGTCAAT AAACCGGTAA ACCAGCAATA GACATAAGCG GCTATTTAAC GACCCTGCCC
     7261 TGAACCGACG ACCGGGTCGA ATTTGCTTTC GAATTTCTGC CATTCATCCG CTTATTATCA
     7321 CTTATTCAGG CGTAGCACCA GGCGTTTAAG GGCACCAATA ACTGCCTTAA AAAAATTACG
     7381 CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA
     7441 AGCCATCACA GACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT
     7501 GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT
     7561 TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA
     7621 TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA
     7681 TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG
     7741 TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT
     7801 CTTTCATTGC CATACG
//