LOCUS       Exported                3210 bp DNA     circular SYN 07-APR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    HA_entry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 7, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    485..1274
                     /label=pUC ori
                     /note="pUC ori"
     terminator      complement(1275..1308)
                     /note="terminator BBa_B1002"
     promoter        complement(1354..1382)
                     /note="S70_F1"
     misc_feature    1393..1412
                     /label=Plasmid target DNA (TS)
                     /note="Plasmid target DNA (TS)"
     misc_feature    1413..1415
                     /label=PAM
                     /note="PAM"
     promoter        1473..1596
                     /note="P lac prom"
     CDS             1603..1926
                     /codon_start=1
                     /label=lacZ-alpha
                     /note="lacZ-alpha"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    1927..2573
                     /label=ccdB Kassette aus pDONR201
                     /note="ccdB Kassette aus pDONR201"
     misc_feature    2268..2573
                     /label=ccdB
                     /note="ccdB"
     CDS             join(2896..3210,1..477)
                     /codon_start=1
                     /label=NPTII
                     /note="NPTII"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc
       61 gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac
      121 ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagagga cactccattt
      181 aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc
      241 ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc
      301 tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc
      361 cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg
      421 gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga attgttttag
      481 cggggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      541 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      601 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      661 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
      721 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
      781 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
      841 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
      901 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
      961 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1021 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1081 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1141 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1201 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1261 gtaaacttgg tctggcgaaa aaaccccgcc gaagcggggt tttttgcggt ctgactgcag
     1321 aagacagccg tcgagttagc tagatatcga tacattataa cagttccttc tgcaaatgtc
     1381 aactcctatg gtaaccaagg tccaaacggt gacggccgtt gagacccagt ggtatgggca
     1441 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     1501 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     1561 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     1621 agcttgcatg cctgcaggtc gactctagag gatccccggg taccgagctc gaattcactg
     1681 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     1741 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     1801 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
     1861 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     1921 gcatagtcgc gtggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct
     1981 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt
     2041 atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg
     2101 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg
     2161 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt
     2221 tattgaaatg aacggctctt ttgctgacga gaacagggac tggtgaaatg cagtttaagg
     2281 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta
     2341 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata
     2401 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga
     2461 ccaccgatat ggccagtgtg cctgtctccg ttatcgggga agaagtggct gatctcagcc
     2521 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taattaagcc
     2581 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     2641 ccgcttacag acaagctgtg acccactcaa gtgggtctca ggagctgtct tccgtgacga
     2701 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     2761 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     2821 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     2881 tgaaaaagga agagtatggc taaaatgaga atatcaccgg aattgaaaaa actgatcgaa
     2941 aaataccgct gcgtaaaaga tacggaagga atgtctcctg ctaaggtata taagctggtg
     3001 ggagaaaatg aaaacctata tttaaaaatg acggacagcc ggtataaagg gaccacctat
     3061 gatgtggaac gggaaaagga catgatgcta tggctggaag gaaagctgcc tgttccaaag
     3121 gtcctgcact ttgaacggca tgatggctgg agcaatctgc tcatgagtga ggccgatggc
     3181 gtcctttgct cggaagagta tgaagatgaa
//
