LOCUS       Exported                3111 bp DNA     circular SYN 07-APR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    HB_entry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 7, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          15
                     /mol_type="other DNA"
                     /organism="pKDsgRNA-ack"
     source          15
                     /mol_type="other DNA"
                     /organism="pTKIP-cat"
     source          1281
                     /mol_type="other DNA"
                     /organism="pTKIP-cat"
     source          1281
                     /mol_type="other DNA"
                     /organism="pKDsgRNA-ack"
     source          1281
                     /mol_type="other DNA"
                     /organism="pKDsgRNA-ack"
     source          1281
                     /mol_type="other DNA"
                     /organism="pTKIP-cat"
     source          1307
                     /mol_type="other DNA"
                     /organism="pTKIP-cat"
     source          1307
                     /mol_type="other DNA"
                     /organism="pKDsgRNA-ack"
     source          1314
                     /mol_type="other DNA"
                     /organism="pKDsgRNA-ack"
     source          1314
                     /mol_type="other DNA"
                     /organism="pTKIP-cat"
     promoter        73..196
                     /note="P lac prom"
     promoter        165
                     /note="P"
     CDS             203..526
                     /codon_start=1
                     /label=lacZ-alpha
                     /note="lacZ-alpha"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    527..1173
                     /label=ccdB Kassette aus pDONR201
                     /note="ccdB Kassette aus pDONR201"
     misc_feature    868..1173
                     /label=ccdB
                     /note="ccdB"
     misc_feature    1288..1307
                     /label=Plasmid target DNA (TS)
                     /note="Plasmid target DNA (TS)"
     CDS             1523..2314
                     /codon_start=1
                     /label=NPTII
                     /note="NPTII"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    2322..3111
                     /label=pUC ori
                     /note="pUC ori"
ORIGIN
        1 gacgaagaca gcgctccgtt gagacccagt ggtatgggca gctggcacga caggtttccc
       61 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
      121 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      181 caatttcaca caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc
      241 gactctagag gatccccggg taccgagctc gaattcactg gccgtcgttt tacaacgtcg
      301 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
      361 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
      421 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
      481 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagtcgc gtggatccgg
      541 cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat
      601 atatactgat atgtataccc gaagtatgtc aaaaagaggt gtgctatgaa gcagcgtatt
      661 acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct
      721 ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga
      781 aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt
      841 ttgctgacga gaacagggac tggtgaaatg cagtttaagg tttacaccta taaaagagag
      901 agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg
      961 atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac
     1021 ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg
     1081 cctgtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa
     1141 aacgccatta acctgatgtt ctggggaata taattaagcc agccccgaca cccgccaaca
     1201 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     1261 acccactcaa gtgggtctca cgctccgtca ccgtttggac cttggttcag tggtctgtct
     1321 tcgacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     1381 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     1441 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1501 ataatattga aaaaggaaga gtatggctaa aatgagaata tcaccggaat tgaaaaaact
     1561 gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa
     1621 gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac
     1681 cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt
     1741 tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctaa tgagtgaggc
     1801 cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga
     1861 gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac
     1921 gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga
     1981 tgtggattgc gaaaactggg aagaggacac tccatttaaa gatccgcgcg agctgtatga
     2041 ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga
     2101 cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg
     2161 cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg
     2221 ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa
     2281 aataaaatat tatattttac tggatgaatt gttttagcgg ggccaggaac cgtaaaaagg
     2341 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2401 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2461 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2521 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2581 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2641 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2701 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2761 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     2821 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2881 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2941 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3001 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3061 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct g
//
