LOCUS       Exported                3231 bp DNA     circular SYN 07-APR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    HAIB_entry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 7, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    410..1199
                     /label=pUC ori
                     /note="pUC ori"
     terminator      complement(1200..1233)
                     /note="terminator BBa_B1002"
     promoter        complement(1257..1298)
                     /note="pS70_16"
     misc_feature    1309..1328
                     /label=Plasmid target DNA (TS)
                     /note="Plasmid target DNA (TS)"
     promoter        1389..1512
                     /note="P lac prom"
     CDS             1519..1842
                     /codon_start=1
                     /label=lacZ-alpha
                     /note="lacZ-alpha"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    1843..2489
                     /label=ccdB Kassette aus pDONR201
                     /note="ccdB Kassette aus pDONR201"
     misc_feature    2184..2489
                     /label=ccdB
                     /note="ccdB"
     misc_feature    2604..2623
                     /label=Plasmid target DNA (TS)
                     /note="Plasmid target DNA (TS)"
     CDS             join(2842..3231,1..402)
                     /codon_start=1
                     /label=NPTII
                     /note="NPTII"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
       61 ctggccgatg tggattgcga aaactgggaa gaggacactc catttaaaga tccgcgcgag
      121 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      181 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
      241 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
      301 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
      361 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagcgggg ccaggaaccg
      421 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      481 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      541 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      601 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      661 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      721 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
      781 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
      841 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
      901 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
      961 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1021 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1081 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1141 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctgg
     1201 cgaaaaaacc ccgccgaagc ggggtttttt gcggtctgac tgcagaagac agccgtcgag
     1261 tacattatac cgtggcacat ttatctgtcc aactcctata tcggcataaa ccaaggtcca
     1321 aacggtgacg gccgttgaga cccagtggta tgggcagctg gcacgacagg tttcccgact
     1381 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     1441 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     1501 ttcacacagg aaacagctat gaccatgatt acgccaagct tgcatgcctg caggtcgact
     1561 ctagaggatc cccgggtacc gagctcgaat tcactggccg tcgttttaca acgtcgtgac
     1621 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     1681 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     1741 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     1801 atatggtgca ctctcagtac aatctgctct gatgccgcat agtcgcgtgg atccggctta
     1861 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1921 actgatatgt atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag
     1981 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     2041 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     2101 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     2161 tgacgagaac agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     2221 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     2281 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     2341 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgcctg
     2401 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     2461 ccattaacct gatgttctgg ggaatataat taagccagcc ccgacacccg ccaacacccg
     2521 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccc
     2581 actcaagtgg gtctcacgct ccgtcaccgt ttggaccttg gttcagtggt ctgtcttccg
     2641 agtcgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     2701 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2761 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2821 taatattgaa aaaggaagag tatggctaaa atgagaatat caccggaatt gaaaaaactg
     2881 atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag
     2941 ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc
     3001 acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt
     3061 ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc
     3121 gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag
     3181 ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg t
//
