LOCUS       Exported                5642 bp DNA     circular SYN 11-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDropVn_entry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 7, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..73
                     /note="tet promoter"
     protein_bind    20..38
                     /note="tet operator"
     protein_bind    45..63
                     /note="tet operator"
     promoter        127..250
                     /note="P lac prom"
     protein_bind    147..168
                     /note="CAP binding site"
     promoter        183..213
                     /note="lac promoter"
     promoter        219
                     /note="P"
     protein_bind    221..237
                     /note="lac operator"
     CDS             257..580
                     /codon_start=1
                     /label=lacZ-alpha
                     /note="lacZ-alpha"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAGHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    274..330
                     /label=MCS
                     /note="MCS"
     misc_feature    581..1227
                     /label=ccdB Kassette aus pDONR201
                     /note="ccdB Kassette aus pDONR201"
     CDS             922..1227
                     /codon_start=1
                     /label=ccdB
                     /note="ccdB"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     misc_feature    1335..1338
                     /label=gRNA
                     /note="gRNA"
     misc_feature    1339..1399
                     /label=gRNA
                     /note="gRNA"
     terminator      1428..1466
                     /note="Terminator (BBa_B1006)"
     misc_feature    1493..1598
                     /label=Synthetic promoter
                     /note="Synthetic promoter"
     RBS             1599..1634
                     /label=sfGFP Ribosome Binding Site
                     /note="sfGFP Ribosome Binding Site"
     CDS             1635..2853
                     /codon_start=1
                     /label=mScarlet-1_V__nat
                     /note="mScarlet-1_V__nat"
                     /translation="MRKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLICP*LLAGF*R*SSDQRIYALQSSHGRFYERPRI*N*R*RRRSSIRRYSNRKTES
                     YQRWSTAIFLGYSVSTIYVRFSCIYQTPSRYSRLLQTIFSGRFQMGTCYEF*RWWCSYG
                     YPRYLSGRWYSDLQS*TAWY*LSTRWSSYAEKNHGLGSIYRTSVPRRWRSER*YQNGTA
                     SERWRSLPGRFQNHLQSEKTSSNARCIQR*S*TGYYQPQRRLHRC*TIRTF*RPSLYRW
                     YG*TVQI"
     misc_feature    1647..2134
                     /label=homology
                     /note="homology"
     misc_feature    2135..2154
                     /label=target sequence
                     /note="target sequence"
     misc_feature    2155..2157
                     /label=PAM
                     /note="PAM"
     misc_feature    2170..2657
                     /label=homology
                     /note="homology"
     terminator      2854..2982
                     /label=BBa_B0015 Terminator
                     /note="BBa_B0015 Terminator"
     CDS             complement(2987..3520)
                     /codon_start=1
                     /label=GM(R)
                     /note="GM"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        3521..3587
                     /label=EM7_promoter
                     /note="EM7_promoter"
     terminator      3603..3654
                     /note="T0 terminator"
     misc_feature    3655..3658
                     /label=GGCA
                     /note="GGCA"
     promoter        3690..3743
                     /note="tet promoter"
     protein_bind    3690..3708
                     /note="tet operator"
     protein_bind    3715..3733
                     /note="tet operator"
     misc_feature    3764..3846
                     /label=gRNA
                     /note="gRNA"
     terminator      3851..3929
                     /note="rrnB_T1_terminator"
     rep_origin      4381..4603
                     /label=PSC101 origin
                     /note="PSC101 origin"
     CDS             4651..5601
                     /codon_start=1
                     /label=ORF frame 3
                     /note="Rep101-ts"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFKIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     misc_feature    4817
                     /label=nots mutation T->C
                     /note="nots mutation T->C"
     misc_feature    4927
                     /label=G->A E93K
                     /note="G->A E93K"
     misc_feature    5612..5642
                     /label=Terminator Bba1002
                     /note="Terminator Bba1002"
ORIGIN
        1 tcgctgtagc acctgaagtt ccctatcagt gatagagatt gacatcccta tcagtgatag
       61 agatactgag cactgagacc cagtggtatg ggcagctggc acgacaggtt tcccgactgg
      121 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      181 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      241 cacacaggaa acagctatga ccatgattac gccaagcttg catgcctgca ggtcgactct
      301 agaggatccc cgggtaccga gctcgaattc actggccgtc gttttacaac gtcgtgactg
      361 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagga catccccctt tcgccagctg
      421 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
      481 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
      541 atggtgcact ctcagtacaa tctgctctga tgccgcatag tcgcgtggat ccggcttact
      601 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
      661 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
      721 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
      781 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
      841 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
      901 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
      961 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg
     1021 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
     1081 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgcctgtc
     1141 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     1201 attaacctga tgttctgggg aatataatta agccagcccc gacacccgcc aacacccgct
     1261 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgacccac
     1321 tcaagtgggt ctcagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     1381 caacttgaaa aagtggcacc gagtcggtgc tttttttacg gtcatgtaaa aaaaaacccc
     1441 gcccctgaca gggcggggtt ttttttcagt caagcctctc ttgacatcgc tagaaattgt
     1501 cttaagatat gttgcttatt taatgaaacg gcaggttctc ttgacatcgc tagaaattgt
     1561 cttaagatat gttgcttatt taatgaaacg gcaggttcgg atcttagcta ctagagaaag
     1621 aggagaaata ctagatgcgt aaaggcgaag cagtgatcaa agaatttatg cgcttcaaag
     1681 ttcacatgga aggttctatg aacggccacg aatttgaaat tgaaggtgaa ggcgaaggtc
     1741 gtccatacga aggtactcaa accgcaaaac tgaaagttac caaaggtggt ccactgccat
     1801 tttcttggga tattctgtct ccacaattta tgtacggttc tcgtgcattt atcaaacacc
     1861 cagcagatat tccagactac tacaaacaat cttttccgga aggtttcaaa tgggaacgtg
     1921 ttatgaattt tgaagatggt ggtgcagtta cggttaccca agatacctct ctggaagatg
     1981 gtactctgat ctacaaagtt aaactgcgtg gtactaactt tccaccagat ggtccagtta
     2041 tgcagaaaaa aaccatgggt tgggaagcat ctaccgaacg tctgtaccca gaagatggcg
     2101 ttctgaaagg tgatatcaaa atggcactgc gtctgatatg cccgtagcta ctagcgggtt
     2161 tctaaaggtg aagcagtgat caaagaattt atgcgcttca aagttcacat ggaaggttct
     2221 atgaacggcc acgaatttga aattgaaggt gaaggcgaag gtcgtccata cgaaggtact
     2281 caaaccgcaa aactgaaagt taccaaaggt ggtccactgc cattttcttg ggatattctg
     2341 tctccacaat ttatgtacgg ttctcgtgca tttatcaaac acccagcaga tattccagac
     2401 tactacaaac aatcttttcc ggaaggtttc aaatgggaac gtgttatgaa ttttgaagat
     2461 ggtggtgcag ttacggttac ccaagatacc tctctggaag atggtactct gatctacaaa
     2521 gttaaactgc gtggtactaa ctttccacca gatggtccag ttatgcagaa aaaaaccatg
     2581 ggttgggaag catctaccga acgtctgtac ccagaagatg gcgttctgaa aggtgatatc
     2641 aaaatggcac tgcgtctgaa agatggcggt cgttacctgg cagatttcaa aaccacctac
     2701 aaagcgaaaa aaccagttca aatgccaggt gcatacaacg ttgatcgtaa actggatatt
     2761 accagccaca acgaagatta caccgttgtt gaacaatacg aacgttctga aggccgtcac
     2821 tctaccggtg gtatggatga actgtacaaa taaccaggca tcaaataaaa cgaaaggctc
     2881 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     2941 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta taatgattag gtggcggtgc
     3001 ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca
     3061 ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg
     3121 cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctctccgg
     3181 agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg ggcagaacgt
     3241 aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgca atgaatgtct
     3301 tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta
     3361 catctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag
     3421 ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc
     3481 gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat ggtttagttc ctcaccttgt
     3541 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacaa ctccggcagg
     3601 acttgttcag aacgctcggt cttgcacacc gggcgttttt tctttgtgag tccaggcaag
     3661 ctttcgctaa ggatgatttc tggaattctt ccctatcagt gatagagatt gacatcccta
     3721 tcagtgatag agatactgag cacgctcgat cgctaatcgt tatgttttag agctagaaat
     3781 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
     3841 ttttttcaag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     3901 tttatctgtt gtttgtcggt gaacgctctg ggtgcattag ccagtctgaa tgacctgtca
     3961 cgggataatc cgaagtggtc agactggaaa atcagagggc aggaactgct gaacagcaaa
     4021 aagtcagata gcaccacata gcagacccgc cataaaacgc cctgagaagc ccgtgacggg
     4081 cttttcttgt attatgggta gtttccttgc atgaatccat aaaaggcgcc tgtagtgcca
     4141 tttaccccca ttcactgcca gagccgtgag cgcagcgaac tgaatgtcac gaaaaagaca
     4201 gcgactcagg tgcctgatgg tcggagacaa aaggaatatt cagcgatttg cccgattgcg
     4261 gccgcaaccg agcttgcgag ggtgctactt aagcctttag ggttttaagg tctgttttgt
     4321 agaggagcaa acagcgtttg cgacatcctt ttgtaatact gcggaactga ctaaagtagt
     4381 gagttataca cagggctggg atctattctt tttatctttt tttattcttt ctttattcta
     4441 taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc ggaatttaca
     4501 gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt acagataccc
     4561 acaactcaaa ggaaaaggac tagtaattat cattgactag cccatctcaa ttggtatagt
     4621 gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt caaagcaaat
     4681 gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat tttatgctgt
     4741 gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac ggtatcgttc
     4801 acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta tggtgtatta
     4861 gctaaagcaa ccagagagct gatgacgaga actgtggaaa tcaggaatcc tttggttaaa
     4921 ggctttaaga ttttccagtg gacaaactat gccaagttct caagcgaaaa attagaatta
     4981 gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat aaaatataat
     5041 ctggaacatg ttaagtcttt tgaaaacaaa tactctatga ggatttatga gtggttatta
     5101 aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct tgatgaattt
     5161 aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa ccaatgggtt
     5221 ttgaaaccaa taagtaaaga tttaaacact tacagcaata tgaaattggt ggttgataag
     5281 cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca aatggatctc
     5341 gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc aacaaccatt
     5401 acatcagatt cctacctaca taacggacta agaaaaacac tacacgatgc tttaactgca
     5461 aaaattcagc tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag taagtatgat
     5521 ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa ccacactaga gaacatactg
     5581 gctaaatacg gaaggatctg agtccatacg tcgcaaaaaa ccccgctttc ggcggggttt
     5641 tt
//
