LOCUS       Exported               16996 bp ds-DNA     circular SYN 03-MAR-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 16996)
  AUTHORS   yy
  TITLE     Direct Submission
  JOURNAL   Exported Apr 21, 2022 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16996
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..2341
                     /label=FAST-RED
                     /note="color: #a6acb3"
     primer_bind     2358..2379
                     /label=BsaI_GGAG_Pro_F
                     /note="color: black; sequence: 
                     AAGGTCTCAGGAGGAACCGCAACGTTGAAGG"
     promoter        2362..2540
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        2394..2576
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     2523..2543
                     /label=Nos_promoter_F
                     /note="color: black; sequence: CCCCTCGGTATCCAATTAGAG"
     terminator      3176..3428
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
     primer_bind     complement(3362..3382)
                     /label=Nos_Ter_R
                     /note="color: black; sequence: TGCGCGCTATATTTTGTTTTC"
     primer_bind     complement(3404..3432)
                     /label=BsaI_CGCT_Ter_R
                     /note="color: black; sequence: 
                     AAGGTCTCAAGCGGATCTAGTAACATAGATGACACCGC"
     misc_feature    3453..4087
                     /label=E9 terminator
                     /note="color: #a6acb3"
     primer_bind     3999..4020
                     /label=rbcs check R
                     /note="color: black; sequence: CTCAGTAGGATTCTGGTGTGTG"
     primer_bind     4036..4057
                     /label=E9_ter_R
                     /note="color: black; sequence: GCATTGAACTTGACGAACGTTG"
     primer_bind     complement(4036..4054)
                     /label=pRW sequencing F2
                     /note="color: black; sequence: CGTTCGTCAAGTTCAATGC"
     misc_feature    4088..4418
                     /label=L2f
                     /label=nonstandard type: Seq.
                     /note="color: #a6acb3"
     misc_feature    complement(4088..4141)
                     /label=c-term. NLS
                     /label=nonstandard type: NLS
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(join(4142..6808,6998..8434))
                     /codon_start=1
                     /label=nCas9-3-NG
                     /note="This feature has 2 segments:
                        1: 4142 .. 6808 / #993366
                        2: 6998 .. 8434 / #993366"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGDG"
     primer_bind     complement(4255..4274)
                     /label=pRW sequencing F
                     /note="color: black; sequence: GTACTTCGATACCACCATCG"
     primer_bind     complement(4624..4644)
                     /label=BC_B58
                     /note="color: black; sequence: CTCTTTTCGAGCTTGAGAACG"
     primer_bind     4954..4981
                     /label=Cas9_Check_R
                     /note="color: black; sequence: 
                     CGGTTTTCTTAACGATGTTAACCTGTGG"
     primer_bind     complement(5025..5044)
                     /label=BC_B30
                     /note="color: black; sequence: ACAGGAGAGATCGTTTGGGA"
     primer_bind     complement(5410..5430)
                     /label=BC_B57
                     /note="color: black; sequence: ACACCAAGTACGATGAGAACG"
     primer_bind     5460..5479
                     /label=BC_B29rev
                     /note="color: black; sequence: TGCTTGGTGATCTGTCTGGT"
     primer_bind     complement(5629..5651)
                     /label=BC_B29.2
                     /note="color: black; sequence: GTCTGATAACGTTCCATCTGAGG"
     primer_bind     complement(6022..6044)
                     /label=Cas9 check F
                     /note="color: black; sequence: GGGAATCCTTCAGACCGTTAAGG"
     primer_bind     complement(6224..6246)
                     /label=BC_B29
                     /note="color: black; sequence: ACGGAATCAGAGATAAGCAGTCT"
     primer_bind     6668..6687
                     /label=BC_B28rev
                     /note="color: black; sequence: AACCTTGGTAAGCTCGTTGT"
     primer_bind     complement(6873..6892)
                     /label=BC_B56
                     /note="color: black; sequence: GCAATTGCTTTTCTGTAGTT"
     primer_bind     complement(7401..7420)
                     /label=BC_B28
                     /note="color: black; sequence: AGACAGCAGCTTCCAGAGAA"
     primer_bind     complement(7653..7674)
                     /label=BC_B55
                     /note="color: black; sequence: ACTTCGATCTTGCTGAGGATGC"
     primer_bind     7864..7884
                     /label=BC_B27rev
                     /note="color: black; sequence: AGGTCTGAACAAGCTGGATGA"
     misc_feature    7917..8833
                     /label=B27r
                     /label=nonstandard type: Seq.
                     /note="color: #a6acb3"
     primer_bind     complement(8409..8435)
                     /label=Cas9ng_moclo_R
                     /note="color: black; sequence: 
                     AAGGTCTCCGACAAGAAGTACTCTATCGGACTTGC"
     CDS             complement(8435..9625)
                     /codon_start=1
                     /label=TadA_Hv
                     /note="color: #993366"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEGW
                     NRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRVVF
                     GARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKKAQ
                     SSTDSGGSSGGSSGSETPGTSESATPESSGGSSGGSSEVEFSHEYWMRHALTLAKRARD
                     EREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVT
                     FEPCVMCAGAMIHSRIGRVVFGVRNAKTGAAGSLMDVLHYPGMNHRVEITEGILADECA
                     ALLCYFFRMPRQVFNAQKKAQSSTDSGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     primer_bind     9028..9046
                     /label=Linker_moclo_R
                     /note="color: black; sequence: AAGGTCTCAAGATCCGCCGCTAGACCC"
     misc_feature    9125..9625
                     /label=TadA_WT
                     /note="color: #a6acb3"
     misc_feature    complement(9327..9626)
                     /label=L2r
                     /label=nonstandard type: Seq.
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    9627..11318
                     /label=RPS5a promoter
                     /note="color: #a6acb3"
     primer_bind     complement(9686..9705)
                     /label=RPS5A Sequence F
                     /note="color: black; sequence: CTTTCTCCAAATCCTCTCTC"
     primer_bind     complement(9713..9732)
                     /label=RPS5A Sequence F2
                     /note="color: black; sequence: CGACGCAAACGATCTTCAGG"
     primer_bind     10426..10445
                     /label=AtRPS5A_promoter_R
                     /note="color: black; sequence: CACTCCATTGATGTTCCGTG"
     misc_feature    11339..11540
                     /label=pU6-26
                     /note="color: #a6acb3"
     primer_bind     11401..11419
                     /label=U6 promoter sgRNA sequencing_2
                     /note="color: black; sequence: AAGCAGGCCCATTTATATG"
     primer_bind     11447..11466
                     /label=U6 promoter sgRNA sequencing
                     /note="color: black; sequence: GGCCCATTTAAGTTGAAAAC"
     primer_bind     complement(11522..11551)
                     /label=BsaI_p4_F
                     /note="color: black; sequence: 
                     aaGGTCTCaCAATcactacttcgactctagct"
     primer_bind     11541..11566
                     /label=addLacZ_F
                     /note="color: black; sequence: 
                     ATGAAGACAAATTGTGAGACCGTCACAGCTTGTCTG"
     CDS             complement(11641..11964)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /note="color: #993366"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     11646..11665
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /note="color: black; sequence: CGGCATCAGAGCAGATTGTA"
     primer_bind     11822..11841
                     /label=pMLBart Seq-F
                     /note="color: black; sequence: GCTGCAAGGCGATTAAGTTG"
     primer_bind     11854..11873
                     /label=Fw_NC
                     /note="color: black; sequence: GTTTTCCCAGTCACGACGTT"
     primer_bind     11859..11881
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
                     /note="color: black; sequence: CCCAGTCACGACGTTGTAAAACG"
     primer_bind     complement(11873..11890)
                     /label=M13-F-Reverse Complement
                     /note="color: black; sequence: ACTGGCCGTCGTTTTACA"
     primer_bind     11873..11890
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
                     /note="color: black; sequence: TGTAAAACGACGGCCAGT"
     primer_bind     11874..11891
                     /label=FW-1-K153A: M13 fwd
                     /note="color: black; sequence: GTAAAACGACGGCCAGTG"
     primer_bind     11874..11890
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    11891..11947
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
                     /note="color: #99ccff"
     primer_bind     complement(11960..11976)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     11960..11976
                     /label=M13-R-Reverse Complement
                     /note="color: black; sequence: GTCATAGCTGTTTCCTG"
     primer_bind     complement(11960..11976)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     complement(11973..11995)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG"
     protein_bind    11984..12000
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(12008..12038)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 12008 .. 12014 / #ffffff / -10
                        2: 12015 .. 12032 / #ffffff
                        3: 12033 .. 12038 / #ffffff / -35"
     primer_bind     complement(12008..12026)
                     /label=pMLBart Seq-R
                     /note="color: black; sequence: GCTTCCGGCTCGTATGTTG"
     primer_bind     complement(12011..12031)
                     /label=pGEMTEasy insert_R
                     /note="color: black; sequence: TTTATGCTTCCGGCTCGTATG"
     protein_bind    12053..12074
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     complement(12121..12140)
                     /label=addLacZ_R
                     /note="color: black; sequence: 
                     ATGAAGACAAAAACTGAGACCGCAGCTGGC"
     primer_bind     12130..12159
                     /label=BsaI_p4_R
                     /note="color: black; sequence: 
                     aaGGTCTCaGTTTAAGAGCTATGCTGGAAACA"
     primer_bind     12136..12156
                     /label=AarI_add
                     /note="color: black; sequence: 
                     tgtggtctcaATTGaaatgcaggtgatgactcacctgcataaGTTTAAGAGCTATGCTG
                     GAA"
     primer_bind     12137..12156
                     /label=pguide1_FOR
                     /note="color: black; sequence: 
                     TGTGGTCTCAATTGGGAATTGGTAAGACTACCATGTTTAAGAGCTATGCTGGAA"
     primer_bind     12137..12156
                     /label=pguide2_FOR
                     /note="color: black; sequence: 
                     TGTGGTCTCAATTGGGTAAGACTACCATTGCTAGGTTTAAGAGCTATGCTGGAA"
     primer_bind     12137..12156
                     /label=R422Gguide_FOR
                     /note="color: black; sequence: 
                     TGTGGTCTCAATTGAGTGAGATATGAAAGATTGTGTTTAAGAGCTATGCTGGAA"
     primer_bind     12183..12208
                     /label=pICH47751_sgRNA_check_F
                     /note="color: black; sequence: AGTCCGTTATCAACTTGAAAAAGTGG"
     primer_bind     complement(12274..12293)
                     /label=sgRNA_R
                     /note="color: black; sequence: 
                     tgtggtctcaAGCGACCCCAGAAATTGAAC"
     primer_bind     complement(12346..12370)
                     /label=pICH47751_check_F
                     /note="color: black; sequence: CGCCAATATATCCTGTCAAACACTG"
     misc_feature    12353..12377
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     primer_bind     complement(12356..12378)
                     /label=MoClo_check_F
                     /note="color: black; sequence: GGTTTACCCGCCAATATATCCTG"
     CDS             12657..13286
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /note="color: #993366"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13715..14788
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /note="color: #993366"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     primer_bind     14221..14235
                     /label=pICH47751_check_R
                     /note="color: black; sequence: AACTTAATAACACATTGCGGACGT"
     rep_origin      14854..15048
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /note="color: #ffff00"
     promoter        15110..15214
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             15215..16009
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     16012..16046
                     /label=HR4 ICE106 R
                     /note="color: black; sequence: 
                     GTCAGACCAAGTTTACTCATATATACTTTAGATTG"
     rep_origin      16180..16768
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     16669..16688
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     primer_bind     16673..16694
                     /label=pYYL001_Seq_R_1
                     /note="color: black; sequence: AACGCCTGGTATCTTTATAGTC"
     primer_bind     16845..16868
                     /label=MoClo_check_R
                     /note="color: black; sequence: CCTGTATCGAGTGGTGATTTTGTG"
     primer_bind     16845..16868
                     /label=pICH47751_sgRNA_check_R
                     /note="color: black; sequence: CCTGTATCGAGTGGTGATTTTGTG"
     misc_feature    16904..16928
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     primer_bind     16945..16968
                     /label=pICH47751_check_R
                     /note="color: black; sequence: AACTTAATAACACATTGCGGACGT"
ORIGIN
        1 tgccgaattc ggatccggag ttctagaatg tcgcggaaca aattttaaaa ctaaatccta
       61 aatttttcta attttgttgc caatagtgga tatgtgggcc gtatagaagg aatctattga
      121 aggcccaaac ccatactgac gagcccaaag gttcgttttg cgttttatgt ttcggttcga
      181 tgccaacgcc acattctgag ctaggcaaaa aacaaacgtg tctttgaata gactcctctc
      241 gttaacacat gcagcggctg catggtgacg ccattaacac gtggcctaca attgcatgat
      301 gtctccattg acacgtgact tctcgtctcc tttcttaata tatctaacaa acactcctac
      361 ctcttccaaa atatatacac atctttttga tcaatctctc attcaaaatc tcattctctc
      421 tagtaaacaa gaacaaaaaa atggcggata cagctagagg aacccatcac gatatcatcg
      481 gcagagatca gtacccgatg atgggccgag atcgtgacca gtaccagatg tccggacgag
      541 gatctgacta ctccaagtct aggcagattg ctaaagctgc aactgctgtc acagctggtg
      601 gttccctcct tgttctctcc agccttaccc ttgttggaac tgtcatagct ttgactgttg
      661 caacacctct gctcgttatc ttcagcccaa tccttgtccc ggctctcatc acagttgcac
      721 tcctcatcac cggttttctt tcctctggag ggtttggcat tgccgctata accgttttct
      781 cttggattta caagtaagca cacatttatc atcttacttc ataattttgt gcaatatgtg
      841 catgcatgtg ttgagccagt agctttggat caattttttt ggtcgaataa caaatgtaac
      901 aataagaaat tgcaaattct agggaacatt tggttaacta aatacgaaat ttgacctagc
      961 tagcttgaat gtgtctgtgt atatcatcta tataggtaaa atgcttggta tgatacctat
     1021 tgattgtgaa taggtacgca acgggagagc acccacaggg atcagacaag ttggacagtg
     1081 caaggatgaa gttgggaagc aaagctcagg atctgaaaga cagagctcag tactacggac
     1141 agcaacatac tggtggggaa catgaccgtg accgtactcg tggtggccag cacactacta
     1201 tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca
     1261 accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
     1321 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca
     1381 gcttcatgta cggcagcaga accttcatca accacaccca gggcatcccc gacttcttta
     1441 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gatgggggcg
     1501 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
     1561 tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
     1621 aggccaacac cgagatgctg taccccgctg acggcggcct ggaaggcaga agcgacatgg
     1681 ccctgaagct cgtgggcggg ggccacctga tctgcaactt caagaccaca tacagatcca
     1741 agaaacccgc taagaacctc aagatgcccg gcgtctacta tgtggaccac agactggaaa
     1801 gaatcaagga ggccgacaaa gaaacctacg tcgagcagca cgaggtggct gtggccagat
     1861 actgcgacct ccctagcaaa ctggggcaca agtgagctta ccccactgat gtcatcgtca
     1921 tagtccaata actccaatgt cggggagtta gtttatgagg aataaagtgt ttagaatttg
     1981 atcaggggga gataataaaa gccgagtttg aatctttttg ttataagtaa tgtttatgtg
     2041 tgtttctata tgttgtcaaa tggtaccatg ttttttttcc tctctttttg taacttgcaa
     2101 gtgttgtgtt gtactttatt tggcttcttt gtaagttggt aacggtggtc tatatatgga
     2161 aaaggtcttg ttttgttaaa cttatgttag ttaactggat tcgtctttaa ccacaaaaag
     2221 ttttcaataa gctacaaatt tagacacgca agccgatgca gtcattagta catatattta
     2281 ttgcaagtga ttacatggca acccaaactt caaaaacagt aggttgctcc atttagtcgc
     2341 tgcaagaatt caagcttgga ggaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2401 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2461 taaggtcact atcagctagc aaatatttct tgtcaaaatg ctccactgac gttccataaa
     2521 ttcccctcgg tatccaatta gagtctcata ttcactctca atccaaataa tctgcaccgg
     2581 atctggatcg tttcgcatgt ctcctgaaag aaggcctgct gatattagaa gggctactga
     2641 ggctgatatg cctgctgtgt gcactattgt gaatcattac attgagactt ctactgtgaa
     2701 tttcaggact gagcctcaag aaccacaaga gtggactgat gatcttgtga ggcttagaga
     2761 gaggtatcct tggcttgttg ctgaagtgga tggtgaagtg gctggtattg cttatgctgg
     2821 tccttggaag gctaggaatg cttacgattg gactgctgag tctactgttt acgtgtctcc
     2881 taggcatcaa agaactggtc ttggatctac tctttacact catcttttga agtctttgga
     2941 ggctcagggt ttcaagtctg tggttgctgt gattggactt cctaatgatc cttctgtgag
     3001 gatgcatgag gctcttggtt atgctcctag gggaatgctt agagctgctg gtttcaaaca
     3061 tggaaattgg catgatgtgg gattctggca gcttgatttc tctcttcctg ttcctcctag
     3121 acctgttctt cctgtgactg agatttgagc gggactctgg atctagagtc aagcagatcg
     3181 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     3241 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     3301 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     3361 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     3421 actagatccg ctactagaat tcgagctcag cgttgtcaat caattggcaa gtcataaaat
     3481 gcattaaaaa atattttcat actcaactac aaatccatga gtataactat aattataaag
     3541 caatgattag aatctgacaa ggattctgga aaattacata aaggaaagtt cataaatgtc
     3601 taaaacacaa gaggacatac ttgtattcag taacatttgc agcttttcta ggtctgaaaa
     3661 tatatttgtt gcctagtgaa taagcataat ggtacaacta caagtgtttt actcctcata
     3721 ttaacttcgg tcattagagg ccacgatttg acacattttt actcaaaaca aaatgtttgc
     3781 atatctctta taatttcaaa ttcaacacac aacaaataag agaaaaaaca aataatatta
     3841 atttgagaat gaacaaaagg accatatcat tcattaactc ttctccatcc atttccattt
     3901 cacagttcga tagcgaaaac cgaataaaaa acacagtaaa ttacaagcac aacaaatggt
     3961 acaagaaaaa cagttttccc aatgccataa tactcaaact cagtaggatt ctggtgtgtg
     4021 cgcaatgaaa ctgatgcatt gaacttgacg aacgttgtcg aaaccgatga tacgaacgaa
     4081 agcaagctca cttcttcttc ttagcctgtc cagccttctt ggtagcagct ggcctcttgc
     4141 caccatctcc tccaagctga gaaagatcga ttcttgtctc gtaaagtccg gtgatagact
     4201 ggtggataag ggtagcatca agaacctcct tggtagatct gtaaactttt ctatcgatgg
     4261 tggtatcgaa gtacttgaaa gctcttggag caccaaggtt ggtaagggtg aaaaggtgga
     4321 tgatgttctc agcctgctct ctgattggct tatctctgtg cttgttgtaa gcagaaagaa
     4381 ccttatcaag gttagcatca gcaaggataa ctctcttaga gaactcagag atttgctcga
     4441 tgatctcatc aaggtagtgc ttgtgctgct caacgaaaag ctgcttctgc tcgttatcct
     4501 ctggagatcc cttcaacttc tcgtagtgag aagcaaggta aaggaagtta acgtacttag
     4561 atggaagagc aagctcgttt cccttctgaa gaaatctagc agaagcaagc attctctttc
     4621 ttccgttctc aagctcgaaa agagagtact ttggcaactt gatgataaga tccttcttaa
     4681 cctccttgta tcccttagcc tcaaggaaat cgattgggtt cttctcgaaa gaagaacgct
     4741 ccatgatggt gattccaaga agctccttaa cagacttcaa cttcttagac tttcccttct
     4801 caaccttagc aacaacaaga acagagtaag caacggttgg agaaacgaat cctccgtact
     4861 tctttgggtc ccaatccttc tttctagcga tcaacttatc agagtttctc tttggtctga
     4921 tagactcctt agagaatcct ccggtctgaa cctcggtttt cttaacgatg ttaacctgtg
     4981 gcatagaaag aacctttcta acggtagcga aatctcttcc cttatcccaa acgatctctc
     5041 ctgtctctcc gtttgtctcg ataagtggtc tttttctgat ctctccgtta gcaagggtga
     5101 tctcggtctt gaagaagttc atgatgttag agtagaagaa gtacttagcg gtagcctttc
     5161 cgatctcctg ctcagactta gcgatcatct ttctaacatc gtaaaccttg taatctccgt
     5221 aaacgaactc agactccaac tttgggtact tcttgataag agcggttcca acaacagcgt
     5281 taaggtaagc atcgtgagcg tggtggtagt tgttgatctc tctaaccttg tagaactgga
     5341 aatcctttct gaaatcagaa accaacttag acttcaaggt gataacctta acctctctga
     5401 tcaacttatc gttctcatcg tacttggtgt tcatacgaga atcaaggatc tgagcaacgt
     5461 gcttggtgat ctgtctggtt tcaacaagct gtctcttgat gaatccagcc ttatcaagct
     5521 cagaaagtcc tcctctctca gccttggtaa ggttatcgaa ctttctctgg gtgatcaact
     5581 tagcgttaag aagctgtctc cagtagttct tcatcttctt aacaacctcc tcagatggaa
     5641 cgttatcaga ctttcctctg ttcttatcag aacgggtaag aaccttgtta tcgatagaat
     5701 catccttcaa gaaagactgt ggaacgatgt gatcaacatc gtaatcagaa agtctgttga
     5761 tgtcaagctc ctgatcaacg tacatatctc ttccgttctg aaggtagtaa aggtacaact
     5821 tctcgttctg aagctgggtg ttctcaactg ggtgctcctt caagatttga gatccaagct
     5881 ccttgattcc ctcctcgatt ctcttcattc tctcacgaga gttcttctgt cccttctggg
     5941 tggtctggtt ctctctagcc atctcgataa cgatgttctc tggcttgtgt ctacccataa
     6001 ccttaacaag ctcatcaaca accttaacgg tctgaaggat tcccttcttg atagctggag
     6061 atccagcaag gttagcgatg tgctcgtgaa gagaatctcc ctgtccagaa acctgagcct
     6121 tctggatgtc ctccttgaag gtaagagaat catcgtggat aagctgcatg aagtttctgt
     6181 tagcgaatcc atcagacttc aagaaatcaa ggatggtctt tccagactgc ttatctctga
     6241 ttccgttgat caacttacga gaaagtctac cccatccggt gtatcttctt ctcttcaact
     6301 gcttcataac cttatcatcg aaaaggtgag cgtaggtctt gagtctctcc tcgatcatct
     6361 ctctatcctc gaaaagggta agggtaagaa cgatgtcctc aaggatgtcc tcgttctcct
     6421 cgttatcaag gaaatcctta tccttgatga tcttcaaaag atcgtggtag gttccaagag
     6481 aagcgttgaa tctatcctca actccagaga tttcaacaga atcgaagcac tcgatcttct
     6541 tgaagtaatc ctccttcaac tgcttaacgg taacctttct gttggtcttg aaaagaagat
     6601 caacgatagc cttcttctgc tctccagaaa ggaaagctgg ctttctcatt ccctcggtaa
     6661 cgtacttaac cttggtaagc tcgttgtaaa cggtgaagta ctcgtaaaga agagagtgct
     6721 ttggaagaac cttctcgttt ggaaggttct tatcgaagtt ggtcattctc tcgatgaaag
     6781 actgagcaga agctccctta tcaacaacct gcacatcaac aaattttggt catatattag
     6841 aaaagttata aattaaaata tacacactta taaactacag aaaagcaatt gctatatact
     6901 acattctttt attttgaaaa aaatatttga aatattatat tactactaat taatgataat
     6961 tattatatat atatcaaagg tagaagcaga aacttacctc ctcgaagttc caaggggtga
     7021 ttgtctcctc agactttctg gtcatccaag cgaaacgaga gtttcctcta gcaagtggtc
     7081 caacgtagta tgggattctg aaggtaagga tcttctcgat cttctctctg ttatccttca
     7141 agaatgggta gaaatcctcc tgtctacgaa ggatagcgtg aagctctcca aggtggatct
     7201 ggtgtgggat agatccgtta tcgaaggttc tctgctttct aagaagatcc tctctgttca
     7261 acttaacaag aagctcctcg gttccatcca tcttctcaag gattggcttg atgaacttgt
     7321 agaactcctc ctgagaagct cctccatcga tgtatccagc gtatccgttc ttagactgat
     7381 cgaagaagat ttccttgtac ttctctggaa gctgctgtct aacaagagcc ttcaaaaggg
     7441 taagatcctg gtggtgctca tcgtatctct tgatcataga agcagaaagt ggagccttgg
     7501 tgatctcggt gttaactcta aggatgtcag aaagaaggat agcatcagaa aggttcttag
     7561 cagcaaggaa aagatcagcg tactgatctc cgatctgagc aagaaggtta tcaagatcat
     7621 catcgtaggt atccttagaa agctgcaact tagcatcctc agcaagatcg aagttagact
     7681 tgaagtttgg ggtaagtcca agagaaagag cgataaggtt tccgaaaagt ccgttcttct
     7741 tctctcctgg aagctgagcg ataaggttct caagtctacg agacttagaa agtctagcag
     7801 aaaggatagc cttagcatca actccagaag cgttgattgg gttctcctcg aaaagctggt
     7861 tgtaggtctg aacaagctgg atgaacaact tatcaacatc agagttatct gggttaagat
     7921 ctccctcgat aaggaagtgt cctctgaact tgatcatgtg agcaagagca aggtagataa
     7981 gtctaagatc agccttatcg gtagaatcaa ccaacttctt tctaaggtgg tagatggttg
     8041 ggtacttctc gtggtaagca acctcatcaa cgatgtttcc gaagattggg tgtctctcgt
     8101 gcttcttatc ctcctcaaca aggaaagact cctcaagtct gtggaagaaa gaatcatcaa
     8161 ccttagccat ctcgttagag aagatttcct gaaggtagca gattctgttc tttcttctgg
     8221 tgtatcttct tctagcggtt ctcttcaatc tggtagcctc agcggtttct ccagaatcga
     8281 aaagaagagc accgataagg ttcttcttga tagagtgtct atcggtgttt ccaagaacct
     8341 tgaacttctt agatggaacc ttgtactcat cggtgataac agcccatcca acagagttgg
     8401 ttccgatggc aagtccgata gagtacttct tgtcgctgcc gccagagctg ccacccgacg
     8461 attctggcgt cgcgctctcc gaggttcccg gtgtctccga accagaggat ccccctgacg
     8521 agcctccaga atccgtggag gactgggcct tcttctgagc attgaacacc tgcctcggca
     8581 tgcgaaaaaa gtagcaaagc agagcggcac actcgtccgc aagaatgcct tctgttatct
     8641 caactctgtg attcatcccg gggtaatgaa gcacgtccat cagagatcct gccgccccgg
     8701 ttttggcgtt gcgaactcca aaaaccacgc gtccgatccg gctatgaatc atagcaccag
     8761 cacacataac gcaaggctcg aacgtgacat agagtgttgc gtcaataagc ctatagttct
     8821 gcataaccag ccctccctgc ctcaaagcca taatctctgc gtgcgccgta gggtcgtgca
     8881 atccgattgc cctattccag ccttcgccaa tcaccctatt gttcaacacc aggactgcgc
     8941 caacaggaac ctcgcgttcg tctctggccc gcttagccag agtcagggcg tgtctcatcc
     9001 agtactcatg ggagaattca acctcggaag atccgccgct agacccacca gacgactctg
     9061 gtgtcgccga ctccgacgtg cctggagtct cagaacctga cgaccccccg gagctgccac
     9121 cagaatccgt cgatgactgt gccttcttct gcgctttgat ttcttgtctc cgcatccgga
     9181 agaagtcgga aagcagagcc gcgcattcat ccgccaaaat accctcggtg atttccaccc
     9241 tgtggttcat gccaggatgg tgcaaaacgt ccataaggct acctgctgcg cctgtttttg
     9301 catccctagc gccgaacacg acgcgcccaa tgcgggaatg aatcattgcg cccgcgcaca
     9361 tgacacacgg ctcgagagtc acataaagag tagcgtctat caagcgatag ttctgcatca
     9421 ccagaccccc ctgtctaaga gccataatct cagcatgtgc tgttggatcg tgtctgccta
     9481 tcggccgatt ccacccctct ccgataacgc ggttattgtg cacaagcaca gcccccaccg
     9541 gaacttcccg ctcgtcccac gctctcttgg ccaacgtcaa agcgtgccgc atccaatact
     9601 cgtgggaaaa ctcaacttct gacattggct gtggtgagag aaacagagcg tgagctcaaa
     9661 tacacaagta cgaaatcaga gttatgagag aggatttgga gaaagaagat cacctgaaga
     9721 tcgtttgcgt cgcgcgaatg agagagacgg cagagaaaac gagaagcttt aagaggacga
     9781 acaaaaaccc taatgtcact ccttttatat agatgagaaa tttagggttt gggctctaat
     9841 ggagctgtct acatgggctt tcatttcttt atccagtaaa gaaccggttt aacaggttcc
     9901 ctggtttata cgtggtttta gccggttaac ttacggaata ttatttttat cttaagttac
     9961 acaaattaca tcattaatag taaaacaaaa ttttatggtg caatttaagt ttttatgatg
    10021 agtgaaattc gaataatata caaatatttt actgtttagg tttccctaaa cctactttta
    10081 gatatgtaaa ttaaacattt tttttttttt ggttagaacc aagtcagtga tcatagaact
    10141 caaaaagctg gtgtttcaat tggtacaagc atttttattg caagatataa aaattcagaa
    10201 ggacatatcc ttgatgtatg tacatgaagg aaccgatttt gactccaagc tacaaagaag
    10261 aagaagaatg atatacaaag aaaactgcat atcctcacca tgaaattgaa agctagagga
    10321 aggggaataa cagatactct gcgtagaaga gattccatat aaaccgataa tatcctgaga
    10381 tagcttcctg caataatcaa atgtttctac actccgttag atatacactc cattgatgtt
    10441 ccgtgataac gagtttatgt ttgtacaaaa gtattttacc ttggtgtagt ttgctttttc
    10501 tagtacccat gtctaccaaa gatcaacaac aaagatatta gagattaatg aatttggcag
    10561 tgtcaaatgc agaaactaaa gcatatagtt atgaagttct tgtgcattat ctcaaaagta
    10621 tgtaagagat ataagagtat ctcataatgc tttacctgga aaattaagcc tgaagccaag
    10681 atcacatgtg caggaatcat caagctacct ctaaaaacct gttcattgtt aaaggataag
    10741 agggttggtc ttttcaaagc tgcatactga ggaaagtaac aatatcgcaa caaaaataaa
    10801 ttggtggcga tagtttatca agcttctaca tgcagtaatg gtgttcgtgc tttaattaac
    10861 cgaaatagtt aagacggaaa tagatgaatt cacctgaggc atgtagaaag ctagtgatat
    10921 ggctgaaaca taatttacta gcagaagtcc agaaccgagg aatgcaatgt ttctcactcc
    10981 aagttttgtt gccagggttg atatttggaa cctattcaag atcaatcaaa ggcatgatta
    11041 actaaacatg cttgaacaaa cacataaaga caatatatca aacggagctg gttaaacatt
    11101 gtctttgact gtcaaatgga ttgagccaaa gagcggaaag aaaatggtta tgcgtgcagt
    11161 tacttacttt cgatctcctt caacatcagg aaggtccttt gtaatagcaa tgaccagtgc
    11221 aaacagtgtc acaaaagatg tgatgaacgc cacaggtgca ctgcaaatag cgaatcaaaa
    11281 gttgagtaat atatctactc acgattatgg ggagctcctt acgaattccc atggggagca
    11341 tcttcattct taagatatga agataatctt caaaaggccc ctgggaatct gaaagaagag
    11401 aagcaggccc atttatatgg gaaagaacaa tagtatttct tatataggcc catttaagtt
    11461 gaaaacaatc ttcaaaagtc ccacatcgct tagataagaa aacgaagctg agtttatata
    11521 cagctagagt cgaagtagtg attgtgagac cgtcacagct tgtctgtaag cggatgccgg
    11581 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    11641 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    11701 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
    11761 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    11821 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    11881 gacggccagt gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg
    11941 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
    12001 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
    12061 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggca aacctgtcgt
    12121 gccagctgcg gtctcagttt aagagctatg ctggaaacag catagcaagt ttaaataagg
    12181 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttt gcaaaatttt
    12241 ccagatcgat ttcttcttcc tctgttcttc ggcgttcaat ttctggggtc gctcagagag
    12301 gatgcacatg tgaccgaggg acacgaagtg atccgtttaa actatcagtg tttgacagga
    12361 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa
    12421 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc cagccgtgcg gctgcatgaa
    12481 atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa
    12541 gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat
    12601 gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga
    12661 aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc
    12721 tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg
    12781 gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg
    12841 accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg
    12901 acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc
    12961 tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg
    13021 ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg
    13081 cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc
    13141 ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca
    13201 caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg
    13261 ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac
    13321 aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag
    13381 cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac
    13441 caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata
    13501 catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg
    13561 ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca
    13621 tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca
    13681 atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc
    13741 ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc
    13801 cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc
    13861 tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgtcgt cgattaggaa
    13921 gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac
    13981 ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg
    14041 agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc
    14101 ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac
    14161 cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc
    14221 acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga
    14281 cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa
    14341 ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa
    14401 gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat
    14461 gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt
    14521 tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa
    14581 ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt
    14641 caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga
    14701 tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat
    14761 cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct
    14821 agcaggggaa aaaggtcgaa aaagcttctt tcctgtggat agcacgtaca ttgggaaccc
    14881 aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa
    14941 ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa
    15001 ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca
    15061 gcgcacagcc gaacagctgc aaaaagcgcc cacatcaagg ctccgagtgc gcggaacccc
    15121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
    15181 ataaatgctt caataatatt gaaaaaggaa gagtatggct aaaatgagaa tatcaccgga
    15241 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
    15301 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
    15361 gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg
    15421 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
    15481 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga
    15541 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
    15601 ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa
    15661 cgatctggcc gatgtggatt gcgaaaactg ggaagaggac actccattta aagatccgcg
    15721 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
    15781 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
    15841 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgctcag
    15901 ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
    15961 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagc tgtcagacca
    16021 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    16081 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    16141 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    16201 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    16261 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    16321 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    16381 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    16441 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    16501 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    16561 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    16621 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    16681 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    16741 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    16801 gctcggatct gttggaccgg acagtagtca tggttgatgg gctgcctgta tcgagtggtg
    16861 attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg
    16921 gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact
    16981 ggggttgaac actctg
//
