LOCUS       Exported               13746 bp DNA     circular SYN 27-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 27, 2022 from SnapGene 6.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     38..59
                     /label=SQpromoter
     primer_bind     110..189
                     /label=TAC-rbs3000-F
     misc_feature    117..145
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     misc_feature    148..165
                     /label=RBS3001.56
     CDS             166..4272
                     /codon_start=1
                     /label=cas9
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     346..367
                     /label=IR9S1
     primer_bind     691..715
                     /label=222
     primer_bind     978..1006
                     /label=333
     primer_bind     1037..1058
                     /label=IR9S2
     primer_bind     complement(1097..1121)
                     /label=ncas9-2R
     primer_bind     1408..1427
                     /label=444
     primer_bind     1755..1775
                     /label=IR9S3
     primer_bind     2135..2153
                     /label=555
     primer_bind     2419..2437
                     /label=IR9S4
     primer_bind     2765..2788
                     /label=666
     primer_bind     3133..3151
                     /label=IR9S5
     primer_bind     complement(3559..3579)
                     /label=pcas9-R
     primer_bind     4098..4122
                     /label=RNA-HR-R
     primer_bind     4267..4292
                     /label=HR-F
     terminator      4296..4341
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             4383..4411
                     /codon_start=1
                     /gene="pJ23119"
                     /label=pJ23119
                     /translation="LTASSVLGI"
     misc_feature    4418..4437
                     /label=JAGS-target1
     CDS             4418..4437
                     /codon_start=1
                     /gene="cadAspacer"
                     /label=cadAspacer
                     /translation="DNNSTQ"
     CDS             4438..4479
                     /codon_start=1
                     /gene="cas9BIRE"
                     /label=cas9BIRE
                     /translation="VLELEIAS*NKASP"
     CDS             4480..4519
                     /codon_start=1
                     /gene="spy-tem"
                     /label=spy-tem
                     /translation="LST*KSGTESVLF"
     misc_feature    4552..5051
                     /label=L
     misc_feature    4621..5011
                     /label=left gene
     misc_feature    5052..5551
                     /label=R
     misc_feature    5153..5449
                     /label=right gene
     terminator      5668..5695
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(5715..5737)
                     /label=HR-R
     CDS             complement(5938..6789)
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C"
                     /label=replication protein C
                     /protein_id="AFQ55250.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     primer_bind     complement(5961..5979)
                     /label=Dec-F
     primer_bind     complement(6081..6100)
                     /label=RNA-HR-F
     CDS             complement(6776..7615)
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein A"
                     /label=replication protein A
                     /protein_id="AFQ55249.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(7645..7851)
                     /codon_start=1
                     /transl_table=11
                     /product="repressor protein F"
                     /label=oriT
                     /protein_id="AFQ55248.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
     CDS             complement(7853..8065)
                     /codon_start=1
                     /transl_table=11
                     /product="unknown"
                     /label=unknown
                     /note="E"
                     /protein_id="AFQ55247.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
     CDS             complement(8126..10255)
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein A"
                     /label=mobilization protein A
                     /protein_id="AFQ55244.1"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESG
                     HMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAER
                     LPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPK
                     AWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRT
                     DRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGR
                     RSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGV
                     ALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQ
                     LKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHG
                     LVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEY
                     DADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQI
                     EQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQK
                     LASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPA
                     PRQRGMDRGGPDFSM"
     CDS             complement(8126..9097)
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /label=replication protein B
                     /protein_id="AFQ55246.1"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             complement(9094..9507)
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein B"
                     /label=mobilization protein B
                     /protein_id="AFQ55245.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     CDS             10454..10738
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /label=mobilization protein C
                     /protein_id="AFQ55243.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     CDS             complement(11803..12639)
                     /codon_start=1
                     /transl_table=11
                     /product="Sm resistance protein B"
                     /label=Sm resistance protein B
                     /protein_id="AFQ55242.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
     CDS             complement(12639..13442)
                     /codon_start=1
                     /transl_table=11
                     /product="Sm resistance protein A"
                     /label=Sm resistance protein A
                     /protein_id="AFQ55241.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRR
                     GELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPS
                     MGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARV
                     ERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIA
                     NAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     misc_feature    13485..13513
                     /label=promoter
     primer_bind     13522..13541
                     /label=promoterF
ORIGIN
        1 ggactataga ctatactagt atactccgtc tactgtacga tacacttccg ctcaggtcct
       61 tgtcctttaa cgaggattgt tacgtacgct aatggcgtca aaacaaagac tctagattga
      121 caattaatca tcggctcgta taatgtttca ccttatcatc aagatatgga taagaaatac
      181 tcaataggct tagatatcgg cacaaatagc gtcggatggg cggtgatcac tgatgaatat
      241 aaggttccgt ctaaaaagtt caaggttctg ggaaatacag accgccacag tatcaaaaaa
      301 aatcttatag gggctctttt atttgacagt ggagagacag cggaagcgac tcgtctcaaa
      361 cggacagctc gtagaaggta tacacgtcgg aagaatcgta tttgttatct acaggagatt
      421 ttttcaaatg agatggcgaa agtagatgat agtttctttc atcgacttga agagtctttt
      481 ttggtggaag aagacaagaa gcatgaacgt catcctattt ttggaaatat agtagatgaa
      541 gttgcttatc atgagaaata tccaactatc tatcatctgc gaaaaaaatt ggtagattct
      601 actgataaag cggatttgcg cttaatctat ttggccttag cgcatatgat taagtttcgt
      661 ggtcattttt tgattgaggg agatttaaat cctgataata gtgatgtgga caaactattt
      721 atccagttgg tacaaaccta caatcaatta tttgaagaaa accctattaa cgcaagtgga
      781 gtagatgcta aagcgattct ttctgcacga ttgagtaaat caagacgatt agaaaatctc
      841 attgctcagc tccccggtga gaagaaaaat ggcttatttg ggaatctcat tgctttgtca
      901 ttgggtttga cccctaattt taaatcaaat tttgatttgg cagaagatgc taaattacag
      961 ctttcaaaag atacttacga tgatgattta gataatttat tggcgcaaat tggagatcaa
     1021 tatgctgatt tgtttttggc agctaagaat ttatcagatg ctattttact ttcagatatc
     1081 ctaagagtaa atactgaaat aactaaggct cccctatcag cttcaatgat taaacgctac
     1141 gatgaacatc atcaagactt gactctttta aaagctttag ttcgacaaca acttccagaa
     1201 aagtataaag aaatcttttt tgatcaatca aaaaacggat atgcaggtta tattgatggg
     1261 ggagctagcc aagaagaatt ttataaattt atcaaaccaa ttttagaaaa aatggatggt
     1321 actgaggaat tattggtgaa actaaatcgt gaagatttgc tgcgcaagca acggaccttt
     1381 gacaacggct ctattcccca tcaaattcac ttgggtgagc tgcatgctat tttgagaaga
     1441 caagaagact tttatccatt tttaaaagac aatcgtgaga agattgaaaa aatcttgact
     1501 tttcgaattc cttattatgt tggtccattg gcgcgtggca atagtcgttt tgcatggatg
     1561 actcggaagt ctgaagaaac aattacccca tggaattttg aagaagttgt cgataaaggt
     1621 gcttcagctc aatcatttat tgaacgcatg acaaactttg ataaaaatct tccaaatgaa
     1681 aaagtactac caaaacatag tttgctttat gagtatttta cggtttataa cgaattgaca
     1741 aaggtcaaat atgttactga aggaatgcga aaaccagcat ttctttcagg tgaacagaag
     1801 aaagccattg ttgatttact cttcaaaaca aatcgaaaag taaccgttaa gcaattaaaa
     1861 gaagattatt tcaaaaaaat agaatgtttt gatagtgttg aaatttcagg agttgaagat
     1921 agatttaatg cttcattagg tacctaccat gatttgctaa aaattattaa agataaagat
     1981 tttttggata atgaagaaaa tgaagatatc ttagaggata ttgttttaac attgacctta
     2041 tttgaagata gggagatgat tgaggaaaga cttaaaacat atgctcacct ctttgatgat
     2101 aaggtgatga aacagcttaa acgtcgccgt tatactggtt ggggacgttt gtctcgaaaa
     2161 ttgattaatg gtattaggga taagcaatct ggcaaaacaa tattagattt tttgaaatca
     2221 gatggttttg ccaatcgcaa ttttatgcag ctgatccatg atgatagttt gacatttaaa
     2281 gaagacattc aaaaagcaca agtgtctgga caaggcgata gtttacatga acatattgca
     2341 aatttagctg gtagccctgc tattaaaaaa ggtattttac agactgtaaa agttgttgat
     2401 gaattggtca aagtaatggg gcggcataag ccagaaaata tcgttattga aatggcacgt
     2461 gaaaatcaga caactcaaaa gggccagaaa aattcgcgag agcgtatgaa acgaatcgaa
     2521 gaaggtatca aagaattagg aagtcagatt cttaaagagc atcctgttga aaatactcaa
     2581 ttgcaaaatg aaaagctcta tctctattat ctccaaaatg gaagagacat gtatgtggac
     2641 caagaattag atattaatcg tttaagtgat tatgatgtcg atcacattgt tccacaaagt
     2701 ttccttaaag acgattcaat agacaataag gtcttaacgc gttctgataa aaatcgtggt
     2761 aaatcggata acgttccaag tgaagaagta gtcaaaaaga tgaaaaacta ttggagacaa
     2821 cttctaaacg ccaagttaat cactcaacgt aagtttgata atttaacgaa agctgaacgt
     2881 ggaggtttga gtgaacttga taaagctggt tttatcaaac gccaattggt tgaaactcgc
     2941 caaatcacta agcatgtggc acaaattttg gatagtcgca tgaatactaa atacgatgaa
     3001 aatgataaac ttattcgaga ggttaaagtg attaccttaa aatctaaatt agtttctgac
     3061 ttccgaaaag atttccaatt ctataaagta cgtgagatta acaattacca tcatgcccat
     3121 gatgcgtatc taaatgccgt cgttggaact gctttgatta agaaatatcc aaaacttgaa
     3181 tcggagtttg tctatggtga ttataaagtt tatgatgttc gtaaaatgat tgctaagtct
     3241 gagcaagaaa taggcaaagc aaccgcaaaa tatttctttt actctaatat catgaacttc
     3301 ttcaaaacag aaattacact tgcaaatgga gagattcgca aacgccctct aatcgaaact
     3361 aatggggaaa ctggagaaat tgtctgggat aaagggcgag attttgccac agtgcgcaaa
     3421 gtattgtcca tgccccaagt caatattgtc aagaaaacag aagtacagac aggcggattc
     3481 tccaaggagt caattttacc aaaaagaaat tcggacaagc ttattgctcg taaaaaagac
     3541 tgggatccaa aaaaatatgg tggttttgat agtccaacgg tagcttattc agtcctagtg
     3601 gttgctaagg tggaaaaagg gaaatcgaag aagttaaaat ccgttaaaga gttactaggg
     3661 atcacaatta tggaaagaag ttcctttgaa aaaaatccga ttgacttttt agaagctaaa
     3721 ggatataagg aagttaaaaa agacttaatc attaaactac ctaaatatag tctttttgag
     3781 ttagaaaacg gtcgtaaacg gatgctggct agtgccggag aattacaaaa aggaaatgag
     3841 ctggctctgc caagcaaata tgtgaatttt ttatatttag ctagtcatta tgaaaagttg
     3901 aagggtagtc cagaagataa cgaacaaaaa caattgtttg tggagcagca taagcattat
     3961 ttagatgaga ttattgagca aatcagtgaa ttttctaagc gtgttatttt agcagatgcc
     4021 aatttagata aagttcttag tgcatataac aaacatagag acaaaccaat acgtgaacaa
     4081 gcagaaaata ttattcattt atttacgttg acgaatcttg gagctcccgc tgcttttaaa
     4141 tattttgata caacaattga tcgtaaacga tatacgtcta caaaagaagt tttagatgcc
     4201 actcttatcc atcaatccat cactggtctt tatgaaacac gcattgattt gagtcagcta
     4261 ggaggtgact aactcgagta aggatctcca ggcatcaaat aaaacgaaag gctcagtcga
     4321 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tcccggggat
     4381 ccttgacagc tagctcagtc ctaggtataa tactagtgac aacaacagca cgcaacagtt
     4441 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     4501 accgagtcgg tgcttttttt gaattctgta ggctagtccg ggcgcgccga atgtagcatc
     4561 agcaaacact gttcaggctg aagcaggacc ggatcggcgc ccgcagcgac aaccgcatcc
     4621 agcaagcggc gggtcgaata gagctccaca tggcgcgaaa gaatggctac gcggggcacc
     4681 gcaaccatgc ctaggctaca tcctcatgga aactgagcag gggcaactcg ccgtgcccga
     4741 tggatcgctc gatgaaacct gcccgccccg gcgaccgctg ctccagcgct gtccttgcca
     4801 gggcgggcgc agcgctgatc gcggcaaaga gtacctccag ttcccaggga tcgacccgca
     4861 gcgtgccaaa gggattatcc cgcgttgccg gacgccagaa gagattcggc aaccatcctt
     4921 tgttgcgcac tgcgggggcc agataaaacg cgcgacaacg tgcggctacc tccgccgacg
     4981 cgggaatcga aatcacatcc tggttttcca taatacccct ttcaagtcgt tgctgatgcg
     5041 ggccgccacc ccaatcgaca aaagcctccc ctccggacat gtttttcggg tatgcttagg
     5101 cacgcccgcc acgacaggcg atggctttgc ccgtttaaac aggaggatga cagtggaccc
     5161 gaaggccctc aatgcccgtt gtgtagaact ttttcagagc ccggatgtgc gcctgcggat
     5221 gtggaatgcg cggatgttct ggcaggttgg cgatcagatg aatgtcgcgc cgaccgcact
     5281 caccgatccc aaggtggata cctgcgaact ggaagtcatg ctcagcgccg ccgcactgac
     5341 cgattcccag tgtgccgctg aactggacaa acgcgagccc ggccgcgccg ccttcattca
     5401 gcgccaggtc cgggaaggca tgcgaccgct tctgcgtcct gcgcaatagt caaggtcgca
     5461 ggcattgcgt agtagttgca ggcccccgca acatgggtgg tcgccagcgt agcggccccc
     5521 tgtgcggcga cgttgaccgg caacgtacag cgaattccgc aggcattgcg gtaccgtagt
     5581 tgcgggcccc aatagcggcc ccccgtcgac ctgcaaaagc ttgcggccgg tcgtgactgg
     5641 gaaaaccctg gcgactagct tggcactggc tcaccttcgg gtgggccttt ctgcgtttat
     5701 acctaggaag cttacttgtt aacttcagga cctgcaggag cagaagagca tacatctgga
     5761 agcaaagcca ggaaagcggc ctatggagct gtgcggcagc gctcagtagg caatttttca
     5821 aaatattgtt aagccttttc tgagcatggt atttttcatg gtattaccaa ttagcaggaa
     5881 aataagccat tgaatataaa agataaaaat gtcttgttta caatagagtg gggggggtca
     5941 gcctgccgcc ttgggccggg tgatgtcgta cttgcccgcc gcgaactcgg ttaccgtcca
     6001 gcccagcgcg accagctccg gcaacgcctc gcgcacccgc tggcggcgct tgcgcatggt
     6061 cgaaccactg gcctctgacg gccagacata gccgcacaag gtatctatgg aagccttgcc
     6121 ggttttgccg gggtcgatcc agccacacag ccgctggtgc agcaggcggg cggtttcgct
     6181 gtccagcgcc cgcacctcgt ccatgctgat gcgcacatgc tggccgccac ccatgacggc
     6241 ctgcgcgatc aaggggttca gggccacgta caggcgcccg tccgcctcgt cgctggcgta
     6301 ctccgacagc agccgaaacc cctgccgctt gcggccattc tgggcgatga tggatacctt
     6361 ccaaaggcgc tcgatgcagt cctgtatgtg cttgagcgcc ccaccactat cgacctctgc
     6421 cccgatttcc tttgccagcg cccgatagct acctttgacc accatggcat cagcggtgac
     6481 ggcctcccac ttgggttcca ggaacagccg gagctgccgt ccgccttcgg tcttgggttc
     6541 cgggccaagc actaggccat taggcccagc catggccacc agcccttgca ggatgcgcag
     6601 atcatcagcg cccagcggct ccgggccgct gaactcgatc cgcttgccgt cgccgtagtc
     6661 atacgtcacg tccagcttgc tgcgcttgcg ctcgccccgc ttgagggcac ggaacaggcc
     6721 gggggccaga cagtgcgccg ggtcgtgccg gacgtggctg aggctgtgct tgttcttagg
     6781 cttcaccacg gggcaccccc ttgctcttgc gctgcctctc cagcacggcg ggcttgagca
     6841 ccccgccgtc atgccgcctg aaccaccgat cagcgaacgg tgcgccatag ttggccttgc
     6901 tcacaccgaa gcggacgaag aaccggcgct ggtcgtcgtc cacaccccat tcctcggcct
     6961 cggcgctggt catgctcgac aggtaggact gccagcggat gttatcgacc agtaccgagc
     7021 tgccccggct ggcctgctgc tggtcgcctg cgcccatcat ggccgcgccc ttgctggcat
     7081 ggtgcaggaa cacgatagag cacccggtat cggcggcgat ggcctccatg cgaccgatga
     7141 cctgggccat ggggccgctg gcgttttctt cctcgatgtg gaaccggcgc agcgtgtcca
     7201 gcaccatcag gcggcggccc tcggcggcgc gcttgaggcc gtcgaaccac tccggggcca
     7261 tgatgttggg caggctgccg atcagcggct ggatcagcag gccgtcagcc acggcttgcc
     7321 gttcctcggc gctgaggtgc gccccaaggg cgtgcaggcg gtgatgaatg gcggtgggcg
     7381 ggtcttcggc gggcaggtag atcaccgggc cggtgggcag ttcgcccacc tccagcagat
     7441 ccggcccgcc tgcaatctgt gcggccagtt gcagggccag catggattta ccggcaccac
     7501 cgggcgacac cagcgccccg accgtaccgg ccaccatgtt gggcaaaacg tagtccagcg
     7561 gtggcggcgc tgctgcgaac gcctccagaa tattgatagg cttatgggta gccattgatt
     7621 gcctcctttg caggcagttg gtggttaggc gctggcgggg tcactacccc cgccctgcgc
     7681 cgctctgagt tcttccaggc actcgcgcag cgcctcgtat tcgtcgtcgg tcagccagaa
     7741 cttgcgctga cgcatccctt tggccttcat gcgctcggca tatcgcgctt ggcgtacagc
     7801 gtcagggctg gccagcaggt cgccggtctg cttgtccttt tggtctttca tatcagtcac
     7861 cgagaaactt gccggggccg aaaggcttgt cttcgcggaa caaggacaag gtgcagccgt
     7921 caaggttaag gctggccata tcagcgactg aaaagcggcc agcctcggcc ttgtttgacg
     7981 tataaccaaa gccaccgggc aaccaatagc ccttgtcact tttgatcagg tagaccgacc
     8041 ctgaagcgct tttttcgtat tccataaaac ccccttctgt gcgtgagtac tcatagtata
     8101 acaggcgtga gtaccaacgc aagcactaca tgctgaaatc tggcccgccc ctgtccatgc
     8161 ctcgctggcg gggtgccggt gcccgtgcca gctcggcccg cgcaagctgg acgctgggca
     8221 gacccatgac cttgctgacg gtgcgctcga tgtaatccgc ttcgtggccg ggcttgcgct
     8281 ctgccagcgc tgggctggcc tcggccatgg ccttgccgat ttcctcggca ctgcggcccc
     8341 ggctggccag cttctgcgcg gcgataaagt cgcacttgct gaggtcatca ccgaagcgct
     8401 tgaccagccc ggccatctcg ctgcggtact cgtccagcgc cgtgcgccgg tggcggctaa
     8461 gctgccgctc gggcagttcg aggctggcca gcctgcgggc cttctcctgc tgccgctggg
     8521 cctgctcgat ctgctggcca gcctgctgca ccagcgccgg gccagcggtg gcggtcttgc
     8581 ccttggattc acgcagcagc acccacggct gataaccggc gcgggtggtg tgcttgtcct
     8641 tgcggttggt gaagcccgcc aagcggccat agtggcggct gtcggcgctg gccgggtcgg
     8701 cgtcgtactc gctggccagc gtccgggcaa tctgcccccg aagttcaccg cctgcggcgt
     8761 cggccacctt gacccatgcc tgatagttct tcgggctggt ttccactacc agggcaggct
     8821 cccggccctc ggctttcatg tcatccaggt caaactcgct gaggtcgtcc accagcacca
     8881 gaccatgccg ctcctgctcg gcgggcctga tatacacgtc attgccctgg gcattcatcc
     8941 gcttgagcca tggcgtgttc tggagcactt cggcggctga ccattcccgg ttcatcatct
     9001 ggccggtggt ggcgtccctg acgccgatat cgaagcgctc acagcccatg gccttgagct
     9061 gtcggcctat ggcctgcaaa gtcctgtcgt tcttcatcgg gccaccaagc gcagccagat
     9121 cgagccgtcc tcggttgtca gtggcgtcag gtcgagcaag agcaacgatg cgatcagcag
     9181 caccaccgta ggcatcatgg aagccagcat cacggttagc catagcttcc agtgccaccc
     9241 ccgcgacgcg ctccgggcgc tctgcgcggc gctgctcacc tcggcggcta cctcccgcaa
     9301 ctctttggcc agctccaccc atgccgcccc tgtctggcgc tgggctttca gccactccgc
     9361 cgcctgcgcc tcgctggcct gctgggtctg gctcatgacc tgccgggctt cgtcggccag
     9421 tgtcgccatg ctctgggcca gcggttcgat ctgctccgct aactcgttga tgcctctgga
     9481 tttcttcact ctgtcgattg cgttcatggt ctattgcctc ccggtattcc tgtaagtcga
     9541 tgatctgggc gttggcggtg tcgatgttca gggccacgtc tgcccggtcg gtgcggatgc
     9601 cccggccttc catctccacc acgttcggcc ccaggtgaac accgggcagg cgctcgatgc
     9661 cctgcgcctc aagtgttctg tggtcaatgc gggcgtcgtg gccagcccgc tctaatgccc
     9721 ggttggcatg gtcggcccat gcctcgcggg tctgctcaag ccatgccttg ggcttgagcg
     9781 cttcggtctt ctgtgccccg cccttctccg gggtcttgcc gttgtaccgc ttgaaccact
     9841 gagcggcggg ccgctcgatg ccgtcattga tccgctcgga gatcatcagg tggcagtgcg
     9901 ggttctcgcc gccaccggca tggatggcca gcgtatacgg caggcgctcg gcaccggtca
     9961 ggtgctgggc gaactcggac gccagcgcct tctgctggtc gagggtcagc tcgaccggca
    10021 gggcaaattc gacctccttg aacagccgcc cattggcgcg ttcatacagg tcggcagcat
    10081 cccagtagtc ggcgggccgc tcgacgaact ccggcatgtg cccggattcg gcgtgcaaga
    10141 cttcatccat gtcgcgggca tacttgcctt cgcgctggat gtagtcggcc ttggccctgg
    10201 ccgattggcc gcccgacctg ctgccggttt tcgccgtaag gtgataaatc gccatgctgc
    10261 ctcgctgttg cttttgcttt tcggctccat gcaatggccc tcggagagcg caccgcccga
    10321 agggtggccg ttaggccagt ttctcgaaga gaaaccggta agtgcgccct cccctacaaa
    10381 gtagggtcgg gattgccgcc gctgtgcctc catgatagcc tacgagacag cacattaaca
    10441 atggggtgtc aagatggtta aggggagcaa caaggcggcg gatcggctgg ccaagctcga
    10501 agaacaacga gcgcgaatca atgccgaaat tcagcgggtg cgggcaaggg aacagcagca
    10561 agagcgcaag aacgaaacaa ggcgcaaggt gctggtgggg gccatgattt tggccaaggt
    10621 gaacagcagc gagtggccgg aggatcggct catggcggca atggatgcgt accttgaacg
    10681 cgaccacgac cgcgccttgt tcggtctgcc gccacgccag aaggatgagc cgggctgaat
    10741 gatcgaccga gacaggccct gcggggctgc acacgcgccc ccacccttcg ggtaggggga
    10801 aaggccgcta aagcggctaa aagcgctcca gcgtatttct gcggggtttg gtgtggggtt
    10861 tagcgggctt tgcccgcctt tccccctgcc gcgcagcggt ggggcggtgt gtagcctagc
    10921 gcagcgaata gaccagctat ccggcctctg gccgggcata ttgggcaagg gcagcagcgc
    10981 cccacaaggg cgctgataac cgcgcctagt ggattattct tagataatca tggatggatt
    11041 tttccaacac cccgccagcc cccgcccctg ctgggtttgc aggtttgggg gcgtgacagt
    11101 tattgcaggg gttcgtgaca gttattgcag gggggcgtga cagttattgc aggggttcgt
    11161 gacagttagt acgggagtga cgggcactgg ctggcaatgt ctagcaacgg caggcatttc
    11221 ggctgagggt aaaagaactt tccgctaagc gatagactgt atgtaaacac agtattgcaa
    11281 ggacgcggaa catgcctcat gtggcggcca ggacggccag ccgggatcgg gatactggtc
    11341 gttaccagag ccaccgaccc gagcaaaccc ttctctatca gatcgttgac gagtattacc
    11401 cggcattcgc tgcgcttatg gcagagcagg gaaaggaatt gccgggctat gtgcaacggg
    11461 aatttgaaga atttctccaa tgcgggcggc tggagcatgg ctttctacgg gttcgctgcg
    11521 agtcttgcca cgccgagcac ctggtcgctt tcagctgtaa gcgtcgcggt ttctgcccga
    11581 gctgtggggc gcggcggatg gccgaaagtg ccgccttgct ggttgatgaa gtactgcctg
    11641 aacaacccat gcgtcagtgg gtgttgagct tcccgtttca gctgcgtttc ctgtttgggg
    11701 tcgtttgcgg gaaggggcgg aatcctacgc taaggctttg gccagcgata ttctccggtg
    11761 agattgatgt gttcccaggg gataggagaa gtcgcttgat atctagtatg acgtctgtcg
    11821 cacctgcttg atcgcggccg cgatagctag atcgcgttgc tcctcttctc catccgcgtt
    11881 ccaagctgcg gaaaggcacc cataagcgta cgcctggtcg agcaggcgac gcggatcgac
    11941 gtccagcgca cgagagaatg cgtccgccat ctgtgcaatg cgtctaggat cgagacaaag
    12001 gtcgtctctg tcagccggat cgtagaacat attggcggcg ccaaagccca cttcaccgac
    12061 cagaccgacg ggatctatca ccagccagcc gcgactggag aacatgatgt tttcatgatg
    12121 cagatcgcca tgtagcccac gcagttccga ggcattgctc atcatttgat cggctataat
    12181 cgccgcgtgg acgtagtcag tttgacaacc tgcgttttga tcatcgcgcg cccgctgaaa
    12241 caaagctgca aagcgatccc ggatcgggag aagggcagaa ggcaggggtt cctcagatgc
    12301 ggcatacagc ttcgccatta gttccgctgc aatttcggtc gcctggtagt cgccgtgctc
    12361 ggcaacgatg tgagagagca ttcgctcccc ggcatattcg agcaacatca gattgttctc
    12421 acgaccgagc aaccggactg ctcccctccc attgcgccat accagatagt cggccccgcg
    12481 cagttcatca gcaatgtctt ctataggttt caatcccttg acgattgcag gagtcccgtc
    12541 tggcaatgaa actttccaaa cgaggctgga aaaggtgtcc gcaatgagaa caggttgcga
    12601 aacgtgccaa tgagcaggaa aaacaggcgg catgaacatc aaccccaagt cagagggtcc
    12661 aatcgcagat agaaggcaag gcgttcgcgg tcgggggctt cgatccccaa tacattgaat
    12721 aggacagcga aggcgcgctc tgcttcatct ggcgctgccc agttctcttc ggcgttagca
    12781 atcatgagtg ccaaatcggc atagcgatct gctgttccga gccgcccaag gtcgatcaga
    12841 cccgtgcatt gaagagtttt agggtccacc atgaagttcg gcatgcaggg atcaccatgg
    12901 caaacaacca tatcggtgcg ctcttggtcg agccgcaccg gtagctctcg ttcgacacga
    12961 gccaaaagat cgagctgcgg cgtactcttg tcctcgtccg gtaagaagtc gggattgacg
    13021 gcattgcggg acaccacatc aacggcgcgt ccgaacattc gcgacagcct gcgctcaaac
    13081 ggacattgat caaccgatag gctgtgaaca gcgccaagtt gctgccccat tgacggccac
    13141 gctttgagca aatccgctcc agacagatca gccgccggta ctcccggaat tgccgttatc
    13201 accaagcatg caccctcctg ttcctcctgc cagttgatga cctcggggca agccacacct
    13261 cgacctttga gccaaatgag gcggtcacgc tctccagcga gctcaccgcg gcgggaagca
    13321 ggtgcgattt tcgcgaaggc atgcccgtca ccacgtcgaa aaacaaaatc accagattct
    13381 ccgcctctga caggcaacca gtcagaatgc gattcaccaa aaaaaatatt agttcgattc
    13441 aatggaggtt ccttcagttt tctgatgaag cgcgaatata gagaaatatc ccgaatgtgc
    13501 agttatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca
    13561 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac
    13621 tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc
    13681 aaacatgaga attcccctgg atttcactga tgagaatatc gtcggagata aatataataa
    13741 ttccac
//
