LOCUS       pEasyG1_nat      6730 bp    DNA     circular SYN 11-MAR-2002
DEFINITION  PCR template vector pUG66, complete sequence.
ACCESSION   AF298794
VERSION     AF298794.1  GI:11344912
KEYWORDS    .
SOURCE      PCR template vector pUG66 (unknown)
  ORGANISM  PCR template vector pUG66
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Gueldener,U., Heinisch,J., Koehler,G.J., Voss,D. and Hegemann,J.H.
  TITLE     A second set of loxP marker cassettes for Cre-mediated multiple
            gene knockouts in budding yeast
  JOURNAL   Nucleic Acids Res. 30 (6), E23 (2002)
   PUBMED   11884642
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Gueldener,U., Heinisch,J.H., Voss,D., Koehler,G. and Hegemann,J.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2000) Institut fuer Mikrobiologie,
            Heinrich-Heine-Universitaet, Universtitaetsstr. 1, Duesseldorf
            40225, Germany
FEATURES             Location/Qualifiers
     CDS             join(6374..6730,1..504)
                     /cds_type=ORF
                     /label=AmpR
     rep_origin      675..1263
                     /label=Ori
     gene            1627..2808
                     /note="yeast selectable marker conferring nourseothricin
                     resistance"
                     /label=natMX
     terminator      complement(1627..1885)
                     /note="TEF terminator"
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(1892..2464)
                     /gene="nat"
                     /note="confers resistance to nourseothricin"
                     /product="Nourseothricin acetyltransferase"
     promoter        complement(2465..2808)
                     /note="Ashbya gossypii TEF promoter"
                     /label="TEF promoter"
     promoter        2883..3151
                     /label="SNR52 promoter"
     primer_bind     complement(3125..3151)
                     /label=gB
     Placeholder     3152..4689
     CDS             complement(3582..4376)
                     /label="Tn5 neomycin phosphotransferase"
     primer_bind     4690..4714
                     /label=gA
     misc_RNA        4690..4764
                     /label="gRNA scaffold"
     terminator      4769..4788
                     /label="SUP4 terminator"
     rep_origin      4900..6242
                     /label="2u ori"
     promoter        6269..6373
                     /label="AmpR promoter"
ORIGIN
        1 TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG
       61 ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC
      121 CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG
      181 ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA
      241 GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG
      301 CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG
      361 TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC
      421 TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT
      481 GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT
      541 GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC
      601 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG
      661 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA
      721 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG
      781 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG
      841 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG
      901 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA
      961 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC
     1021 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC
     1081 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA
     1141 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT
     1201 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG
     1261 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC
     1321 ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA
     1381 GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG
     1441 GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGG
     1501 TTAACCTGGC TTATCGAAAT TAATACGACT CACTATAGGG AGACCGGCAG ATCCGCGGCC
     1561 GCATAGGCCA CTAGTGGATC TGATATCACC TAATAACTTC GTATAGCATA CATTATACGA
     1621 AGTTATATTA AGGGTTCTCG AGAGCTCGTT TTCGACACTG GATGGCGGCG TTAGTATCGA
     1681 ATCGACAGCA GTATAGCGAC CAGCATTCAC ATACGATTGA CGCATGATAT TACTTTCTGC
     1741 GCACTTAACT TCGCATCTGG GCAGATGATG TCGAGGCGAA AAAAAATATA AATCACGCTA
     1801 ACATTTGATT AAAATAGAAC AACTACAATA TAAAAAAACT ATACAAATGA CAAGTTCTTG
     1861 AAAACAAGAA TCTTTTTATT GTCAGTACTG ATTAGGGGCA GGGCATGCTC ATGTAGAGCG
     1921 CCTGCTCGCC GTCCGAGGCG GTGCCGTCGT ACAGGGCGGT GTCCAGGCCG CAGAGGGTGA
     1981 ACCCCATCCG CCGGTACGCG TGGATCGCCG GTGCGTTGAC GTTGGTGACC TCCAGCCAGA
     2041 GGTGCCCGGC GCCCCGCTCG CGGGCGAACT CCGTCGCGAG CCCCATCAAC GCGCGCCCGA
     2101 CCCCGTGCCC CCGGTGCTCC GGGGCGACCT CGATGTCCTC GACGGTCAGC CGGCGGTTCC
     2161 AGCCGGAGTA CGAGACGACC ACGAAGCCCG CCAGGTCGCC GTCGTCCCCG TACGCGACGA
     2221 ACGTCCGGGA GTCCGGGTCG CCGTCCTCCC CGTCGTCCGA TTCGTCGTCC GATTCGTCGT
     2281 CGGGGAACAC CTTGGTCAGG GGCGGGTCCA CCGGCACCTC CCGCAGGGTG AAGCCGTCCC
     2341 CGGTGGCGGT GACGCGGAAG ACGGTGTCGG TGGTGAAGGA CCCATCCAGT GCCTCGATGG
     2401 CCTCGGCGTC CCCCGGGACA CTGGTGCGGT ACCGGTAAGC CGTGTCGTCA AGAGTGGTAC
     2461 CCATGGTTGT TTATGTTCGG ATGTGATGTG AGAACTGTAT CCTAGCAAGA TTTTAAAAGG
     2521 AAGTATATGA AAGAAGAACC TCAGTGGCAA ATCCTAACCT TTTATATTTC TCTACAGGGG
     2581 CGCGGCGTGG GGACAATTCA ACGCGTCTGT GAGGGGAGCG TTTCCCTGCT CGCAGGTCTG
     2641 CAGCGAGGAG CCGTAATTTT TGCTTCGCGC CGTGCGGCCA TCAAAATGTA TGGATGCAAA
     2701 TGATTATACA TGGGGATGTA TGGGCTAAAT GTACGGGCGA CAGTCACATC ATGCCCCTGA
     2761 GCTGCGCACG TCAAGACTGT CAAGGAGGGT ATTCTGGGCC TCCATGTCGC TGATTACCCT
     2821 GTTATCCCTA CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCGGGTA
     2881 ATTCTTTGAA AAGATAATGT ATGATTATGC TTTCACTCAT ATTTATACAG AAACTTGATG
     2941 TTTTCTTTCG AGTATATACA AGGTGATTAC ATGTACGTTT GAAGTACAAC TCTAGATTTT
     3001 GTAGTGCCCT CTTGGGCTAG CGGTAAAGGT GCGCATTTTT TCACACCCTA CAATGTTCTG
     3061 TTCAAAAGAT TTTGGTCAAA CGCTGTAGAA GTGAAAGTTG GTGCGCATGT TTCGGCGTTC
     3121 GAAACTTCTC CGCAGTGAAA GATAAATGAT CATTTCCCCG AAAAGTGCCA CCTGCATCGA
     3181 TGGCCCCCCG ATGGTAGTGT GGGGTCTCCC CATGCGAGAG TAGGGAACTG CCAGGCATCA
     3241 AATAAAACGA AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT
     3301 GAACGCTCTC CTGAGTAGGA CAAATCCGCC GGGAGCGGAT TTGAACGTTG CGAAGCAACG
     3361 GCCCGGAGGG TGGCGGGCAG GACGCCCGCC ATAAACTGCC AGGCATCAAA TTAAGCAGAA
     3421 GGCCATCCTG ACGGATGGCC TTTTTGCGTG GCCAGTGCCA AGCTTGCATG CAGATTGCAG
     3481 CATTACACGT CTTGAGCGAT TGTGTAGGCT GGAGCTGCTT CGAAGTTCCT ATACTTTCTA
     3541 GAGAATAGGA ACTTCGGAAT AGGAACTTCA AGATCCCCTT ATTAGAAGAA CTCGTCAAGA
     3601 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG
     3661 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC
     3721 TGATAGCGGT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT
     3781 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG
     3841 GGCATGCGCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG
     3901 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA
     3961 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT
     4021 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC
     4081 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA
     4141 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCCTGCAGT
     4201 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC
     4261 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT
     4321 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA
     4381 AACGATCCTC ATCCTGTCTC TTGATCAGAT CTTGATCCCC TGCGCCATCA GATCCTTGGC
     4441 GGCAAGAAAG CCATCCAGTT TACTTTGCAG GGCTTCCCAA CCTTACCAGA GGGCGCCCCA
     4501 GCTGGCAATT CCGGTTCGCT TGCTGTCCAT AAAACCGCCC AGTCTAGCTA TCGCCATGTA
     4561 AGCCCACTGC AAGCTACCTG CTTTCTCTTT GCGCTTGCGT TTTCCCTTGT CCAGATAGCC
     4621 CAGTAGCTGA CATTCATCCG GGGTCAGCAC CGTTTCTGCG GACTGGCTTT CTACGTGTTC
     4681 CGCTTCCTTG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC GTTATCAACT
     4741 TGAAAAAGTG GCACCGAGTC GGTGGTGCTT TTTTTGTTTT TTATGTCTCG GCGAATTGGG
     4801 TGCGGCCGCG TTTGTCACCT TTCATAGGCC TGACGAAAGG GCCTCGTGAT ACGCCTATTT
     4861 TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGTATG ATCCAATATC AAAGGAAATG
     4921 ATAGCATTGA AGGATGAGAC TAATCCAATT GAGGAGTGGC AGCATATAGA ACAGCTAAAG
     4981 GGTAGTGCTG AAGGAAGCAT ACGATACCCC GCATGGAATG GGATAATATC ACAGGAGGTA
     5041 CTAGACTACC TTTCATCCTA CATAAATAGA CGCATATAAG TACGCATTTA AGCATAAACA
     5101 CGCACTATGC CGTTCTTCTC ATGTATATAT ATATACAGGC AACACGCAGA TATAGGTGCG
     5161 ACGTGAACAG TGAGCTGTAT GTGCGCAGCT CGCGTTGCAT TTTCGGAAGC GCTCGTTTTC
     5221 GGAAACGCTT TGAAGTTCCT ATTCCGAAGT TCCTATTCTC TAGAAAGTAT AGGAACTTCA
     5281 GAGCGCTTTT GAAAACCAAA AGCGCTCTGA AGACGCACTT TCAAAAAACC AAAAACGCAC
     5341 CGGACTGTAA CGAGCTACTA AAATATTGCG AATACCGCTT CCACAAACAT TGCTCAAAAG
     5401 TATCTCTTTG CTATATATCT CTGTGCTATA TCCCTATATA ACCTACCCAT CCACCTTTCG
     5461 CTCCTTGAAC TTGCATCTAA ACTCGACCTC TACATTTTTT ATGTTTATCT CTAGTATTAC
     5521 TCTTTAGACA AAAAAATTGT AGTAAGAACT ATTCATAGAG TGAATCGAAA ACAATACGAA
     5581 AATGTAAACA TTTCCTATAC GTAGTATATA GAGACAAAAT AGAAGAAACC GTTCATAATT
     5641 TTCTGACCAA TGAAGAATCA TCAACGCTAT CACTTTCTGT TCACAAAGTA TGCGCAATCC
     5701 ACATCGGTAT AGAATATAAT CGGGGATGCC TTTATCTTGA AAAAATGCAC CCGCAGCTTC
     5761 GCTAGTAATC AGTAAACGCG GGAAGTGGAG TCAGGCTTTT TTTATGGAAG AGAAAATAGA
     5821 CACCAAAGTA GCCTTCTTCT AACCTTAACG GACCTACAGT GCAAAAAGTT ATCAAGAGAC
     5881 TGCATTATAG AGCGCACAAA GGAGAAAAAA AGTAATCTAA GATGCTTTGT TAGAAAAATA
     5941 GCGCTCTCGG GATGCATTTT TGTAGAACAA AAAAGAAGTA TAGATTCTTT GTTGGTAAAA
     6001 TAGCGCTCTC GCGTTGCATT TCTGTTCTGT AAAAATGCAG CTCAGATTCT TTGTTTGAAA
     6061 AATTAGCGCT CTCGCGTTGC ATTTTTGTTT TACAAAAATG AAGCACAGAT TCTTCGTTGG
     6121 TAAAATAGCG CTTTCGCGTT GCATTTCTGT TCTGTAAAAA TGCAGCTCAG ATTCTTTGTT
     6181 TGAAAAATTA GCGCTCTCGC GTTGCATTTT TGTTCTACAA AATGAAGCAC AGATGCTTCG
     6241 TTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT
     6301 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG
     6361 AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC
     6421 ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA
     6481 TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA
     6541 GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG
     6601 CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC
     6661 TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC
     6721 AGTAAGAGAA
//
