LOCUS       pJT305_EF1a-attB-Rec_P 11669 bp ds-DNA     linear       03-FEB-2022
DEFINITION  .
ACCESSION   urn.local...ct-amu5txp                                             
VERSION     urn.local...ct-amu5txp                                             
FEATURES             Location/Qualifiers
     misc_feature    238..617
                     /label="Geneious type: Regulatory_region"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        618..821
                     /label="promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     LTR             835..1015
                     /label="LTR"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2440..2557
                     /label="cPPT"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        2682..3859
                     /label="promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3860..3963
                     /label="Pa01 attB"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             3973..5550
                     /label="CDS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             3973..5550
                     /label="Translation 3973-5550"
     CDS             5566..5619
                     /label="CDS"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             5566..5619
                     /label="Translation 5566-5619"
     CDS             5620..6336
                     /label="EGFP"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    6361..6949
                     /label="WPRE"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     LTR             7474..7654
                     /label="LTR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    7686..7893
                     /label="Geneious type: polyA"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      complement(9917..10505)
                     /label="origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(10676..11536)
                     /label="AmpR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     promoter        complement(11537..11641)
                     /label="promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta atgcaaagat ggataaagtt ttaaacagag aggaatcttt
     2641 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
     2701 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2761 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2821 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2881 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2941 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccact ggctgcagta
     3001 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     3061 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     3121 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     3181 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     3241 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     3301 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     3361 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     3421 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     3481 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     3541 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     3601 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     3661 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     3721 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     3781 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     3841 tccatttcag gtgtcgtgac cggtttccct tcgcacccgc accgcggctt cgagaccgtg
     3901 acctacatgc tcgaagggcg tatgcgccac gaagaccacc tcggcaatcg cggcctgctc
     3961 aagGCCgcca ccatgggccc tagcgccttc agctacgtgc gctttagcag cggcaagcag
     4021 gccaagggca gctctgaaca cagacagaga gccatgctgg gacagtggct ggaacagcac
     4081 cctagcttca cactgagtga tctgcggttc gaggacctgg gcagaagcgg attcagcggc
     4141 gagcacctgg atcacggcct gggccagctg ctggctgcta tcgacagcgg cgccatcaag
     4201 agcggagatg tgatcctggt cgaggccgtg gacagaatcg gcagactgga gcctctggaa
     4261 atgctgcctc tgttctccag aatcgtgaag gccggcgtgt ccgttatcac cctggaagat
     4321 ggccacgttt acgaccggag ctccgtgaac gagacatctc tgtttctgct ggtggccaaa
     4381 atccagcagg cccacgagta ctctaataga ctgtctcgaa gaatcaacgc ctcttacacc
     4441 gctcggagag aaaaagctaa agcaggcctg ggcatcaaga gagagacacc tgtgtggctg
     4501 accacagacg gccaactggt gcctcacgtg gcccctcaca tcgcccaggc ttttcaggac
     4561 tacgccgatg gcctgggaga gagaaggatt tgtagaaagc tgcgggagag cggcctggaa
     4621 gaattctcca agaccaacgc caccaccgtg cggagatggc tgaagaaccg gaccgccatt
     4681 ggatattgga acgacatccc tgatgtgtac cctcatgtcg tggaccctgc tctgttctac
     4741 caggtgcagc agcgcctgga cgcccctaag gtggacagag ccaagcccag cgctcactac
     4801 ctgacaggcc tcgtgaagtg cgccgtgtgc ggcagaaact acaactacaa acagcggaag
     4861 cacaccgacc ccgctatgct gtgcaccagc agagccagac tggccgggga aggctgcagc
     4921 aatagcaaga cataccccgt gatagttctg gaccaggtgc ggaagctgac cagcctgccg
     4981 ttcctgcaac acgccatgga atccgcctca tctcaggccg acccaagcag ccagagactg
     5041 gccgtgatcg acggcgaaat cggcgagctg tccagaaaga tcagcgaggc cacaaaggcc
     5101 ctgctggtgc tgggcttcac ccctgagatc caggagagcc tggaacagct caagaccgcc
     5161 agagaagccc tggaggaaga aagagccacc ctgctgctgc ctcaggccga gaagctgaca
     5221 acagctcagc tggaggcctt cagcaacggc ctgctggacg acgagcccat gaagctcaac
     5281 cacgtgcttc agaccgccgg ctacagcatg gtggtgcacc ccgacggctc tatcgacgtg
     5341 gatggaaaaa gattcgtgta cgagggcgct agccggaaag agaaagtgta caagctgaga
     5401 ctgatcggcg aggataagca gtggagcctg cccatcctga ccccacagat ggccacttac
     5461 aagtccctgt tcatggccgc tgtgcggctg cctggcgacc ccagcgagga agagctgaga
     5521 cggttcgagg aagccaagca cagcgagcgg gctagcggca gtggagaggg cagaggaagt
     5581 ctgctaacat gcggtgacgt cgaggagaat cctggcccag tgagcaaggg cgaggagctg
     5641 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     5701 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     5761 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     5821 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     5881 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     5941 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     6001 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     6061 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     6121 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     6181 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     6241 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     6301 gggatcactc tcggcatgga cgagctgtac aagtaagaat tcgatatcaa gcttatcgat
     6361 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     6421 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6481 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6541 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     6601 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     6661 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     6721 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     6781 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     6841 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     6901 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     6961 cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     7021 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     7081 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7141 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     7201 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
     7261 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
     7321 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
     7381 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
     7441 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga
     7501 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7561 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7621 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc
     7681 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     7741 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     7801 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     7861 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     7921 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     7981 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     8041 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     8101 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     8161 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     8221 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     8281 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     8341 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     8401 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     8461 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     8521 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     8581 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8641 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     8701 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     8761 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     8821 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     8881 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     8941 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     9001 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     9061 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     9121 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     9181 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
     9241 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     9301 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     9361 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9421 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     9481 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     9541 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     9601 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     9661 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     9721 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     9781 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     9841 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9901 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9961 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    10021 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    10081 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    10141 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    10201 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    10261 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    10321 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    10381 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    10441 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    10501 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    10561 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    10621 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    10681 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    10741 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    10801 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    10861 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    10921 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    10981 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    11041 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    11101 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    11161 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    11221 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    11281 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    11341 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    11401 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    11461 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    11521 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    11581 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    11641 gcacatttcc ccgaaaagtg ccacctgac
//