LOCUS       pJT306_EF1a-attB-Rec_K 11672 bp ds-DNA     linear       23-JUL-2021
DEFINITION  .
ACCESSION   urn.local...ck-amu5txl                                             
VERSION     urn.local...ck-amu5txl                                             
FEATURES             Location/Qualifiers
     misc_feature    238..617
                     /label="Geneious type: Regulatory_region"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        618..821
                     /label="promoter"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     LTR             835..1015
                     /label="LTR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2440..2557
                     /label="cPPT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        2682..3859
                     /label="promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3860..3972
                     /label="Kp03 attB"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             3982..5553
                     /label="CDS"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             3982..5553
                     /label="Translation 3982-5553"
     CDS             5554..6339
                     /label="Translation 5554-6339"
     CDS             5569..5622
                     /label="CDS"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             5623..6339
                     /label="EGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    6364..6952
                     /label="WPRE"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     LTR             7477..7657
                     /label="LTR"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    7689..7896
                     /label="Geneious type: polyA"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     rep_origin      complement(9920..10508)
                     /label="origin"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(10679..11539)
                     /label="AmpR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        complement(11540..11644)
                     /label="promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta atgcaaagat ggataaagtt ttaaacagag aggaatcttt
     2641 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
     2701 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2761 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2821 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2881 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2941 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccact ggctgcagta
     3001 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     3061 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     3121 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     3181 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     3241 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     3301 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     3361 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     3421 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     3481 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     3541 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     3601 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     3661 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     3721 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     3781 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     3841 tccatttcag gtgtcgtgat ttgcatttaa aatcggagca tcatttttca acagaaacga
     3901 ctatgagcgc aatgaccctt gatttacctc gccgctttcc gtggccaacg ctgctgtccg
     3961 tggctatcca cgGCCgccac catgggcaga caggtgatca cctacctgcg gttctctagc
     4021 aagccccagg agagaggcga cagcatccgg agacaaaagg gcctgttcga gcggtggctg
     4081 aaggacaacc ccgacgccaa ggtggtggac gagttcagcg acgagggcgc ttcagcttat
     4141 cacggccacc acctgaaggg cgatttcggc agaatgctgc agaacatcca agacggcaag
     4201 tacctgagcg gccagacagt cctgctggtt gagtccgaaa ccagactgaa cagacagaag
     4261 gccagaaaca ccgagaacct ggtcgacctt atcactggaa aaggcgtgga cgtgatctgc
     4321 ctggaaagcg gcaaaatcta caccagtaca aacattgatg acctggatac cagcatccag
     4381 ctgaagatcg ccgctcacat cgcccaccag cagagcaagg aaaagagcat caaggtcagc
     4441 gccgcctggg agcacagagc tcagctggcc ctggaaggca agcagcagct gacaaagaac
     4501 gtgcccggct ggatcgatcc tgacaccaga aagctgaacg agcacagcag caccgtggtg
     4561 accattttcg acctgctgct gagtggcgag agcctgcaca acatcgccag atacctgcag
     4621 gccaacaaca tcaagtcttt tagccggcgg gaaaaggcca atggcttcag cgtgcacagc
     4681 gtgcggacca tcctgagatc tgagagcacc gtgggcaccc ttgctgcctc taagagaaat
     4741 gatagacctg ccatccccaa ctactacgag cctgctatcg acgtggccac cttcaacaag
     4801 gcccaggaga tcctgagcaa aaaccgggtg ggtagagccc ctgccagcga caatcctatc
     4861 acaatcaacc ttttcaaggg catcattaga tgccagtgcg gcgccagcgt gcatcctacc
     4921 ggagttaagg caacctacca gggcgtgtac agatgtaaca acgtgcccga tggcagatgc
     4981 aacgtgccta caatcaaaag aaagcccttc gataagtgga tgctggacaa catagttgga
     5041 tttctggaac gggacgacgg caacaacaca gacaagcgga aggctgaaat cgagtaccaa
     5101 atcagcctgg tgaccagcaa actgaaaaag gccacaacac tgctgctgga actggacgac
     5161 gtgaccgagc tgaaggaaca ggtgaaggag ctgaacatcc agcggagcaa cctgcaatct
     5221 gaactggacg agctcaacca gagagagaca ctgagcgaca agcctctgca ccacctgagc
     5281 gagatcgacc tgaccaccaa ggcaggcaga gtggaagccc agctgatcct gagtaagttc
     5341 gtgcagagca tcgagctcca gagagaaatg atcatcatca ccctgagaaa cggcacagtg
     5401 atcggcaagt ccagagacct gagccctgtg ctgtctcagg acctgatgaa acaggtcgtg
     5461 tcctccccat ctccaaccga catcgacatg ttctcagtga taacaagtga tgaggaattt
     5521 agaaaaagcg gaaaacaggt gaccaagcgg agcgctagcg gcagtggaga gggcagagga
     5581 agtctgctaa catgcggtga cgtcgaggag aatcctggcc cagtgagcaa gggcgaggag
     5641 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     5701 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     5761 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     5821 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     5881 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     5941 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     6001 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     6061 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     6121 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     6181 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     6241 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     6301 gccgggatca ctctcggcat ggacgagctg tacaagtaag aattcgatat caagcttatc
     6361 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     6421 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     6481 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     6541 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     6601 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     6661 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6721 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     6781 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6841 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6901 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     6961 cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     7021 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     7081 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7141 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     7201 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca
     7261 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag
     7321 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat
     7381 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt
     7441 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc
     7501 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7561 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7621 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc
     7681 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     7741 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     7801 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     7861 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     7921 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     7981 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     8041 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     8101 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     8161 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     8221 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     8281 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     8341 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     8401 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8461 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     8521 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     8581 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8641 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     8701 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     8761 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     8821 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
     8881 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     8941 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     9001 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     9061 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     9121 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     9181 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
     9241 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     9301 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     9361 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     9421 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     9481 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     9541 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     9601 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     9661 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     9721 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     9781 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     9841 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     9901 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     9961 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    10021 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    10081 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    10141 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    10201 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    10261 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    10321 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    10381 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    10441 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    10501 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    10561 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    10621 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    10681 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    10741 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    10801 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    10861 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    10921 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    10981 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    11041 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    11101 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    11161 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    11221 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    11281 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    11341 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    11401 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    11461 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    11521 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    11581 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    11641 cgcgcacatt tccccgaaaa gtgccacctg ac
//