LOCUS       pSLS.601                3448 bp    DNA     circular UNA 18-JAN-2022
DEFINITION  pRSFDuet-1 Eco1 ncRNA (variant basis).
ACCESSION   urn.local...25-3x3fjhp
VERSION     urn.local...25-3x3fjhp
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     protein_bind    5..25
                     /vntifkey=31
                     /label="lacO"
     misc_RNA        54..134
                     /created_by="User"
                     /label="msdRNA"
     primer          54..65
                     /created_by="User"
                     /modified_by="Seth"
                     /label="a1"
     misc_feature    complement(124..180)
                     /created_by="User"
                     /label="msDNA"
     motif           141..150
                     /created_by="User"
                     /label="Stem frag"
     motif           151..155
                     /created_by="User"
                     /label="loop"
     motif           complement(156..165)
                     /created_by="User"
                     /label="Stem frag"
     motif           complement(166..180)
                     /created_by="User"
                     /label="Flanking"
     misc_feature    182..193
                     /created_by="User"
                     /modified_by="Seth"
                     /label="a2"
     terminator      219..266
                     /label="T7\Terminator"
     CDS             complement(358..1173)
                     /label="Kan"
     RBS             complement(1179..1183)
     -10_signal      complement(1215..1221)
                     /label="-10\Ap"
     -35_signal      complement(1238..1243)
                     /label="-35\Ap"
     rep_origin      1281..2030
                     /label="RSF\ori\-\RSF1030\Homology"
     misc_RNA        complement(1281..1979)
                     /label="RNA\II"
     -35_signal      1834..1839
                     /label="-35\RNA\I"
     -10_signal      1858..1863
                     /label="-10\RNA\I"
     misc_RNA        1870..1976
                     /label="RNA\I"
     CDS             complement(2226..3308)
                     /note="Wildtype lacI gene"
                     /label="lacI"
     promoter        3432..3448
                     /label="T7\Promoter"
ORIGIN      
        1 ggggaattgt gagcggataa caattcccca tttaactatg ataagattcc gtatgcgcac
       61 ccttagcgag aggtttatca ttaaggtcaa cctctggatg ttgtttcggc atcctgcatt
      121 gaatctgagt tacaagctgt ttgtcgccag tcagactggc gacaacccgt tttttctgac
      181 gtaagggtgc gcaactttca tgaaatccgc tgcatcacct agcataaccc cttggggcct
      241 ctaaacgggt cttgaggggt tttttgaagt ggcacttttc ggggaaatgt gcgcggaacc
      301 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgaa ttaattctta
      361 gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc
      421 atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag
      481 gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat
      541 taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga
      601 atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc
      661 attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc
      721 ctgagcgaga cgaaatacgc ggtcgctgtt aaaaggacaa ttacaaacag gaatcgaatg
      781 caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc
      841 ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc
      901 aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag
      961 tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa
     1021 ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt
     1081 atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggcct
     1141 agagcaagac gtttcccgtt gaatatggct catactcttc ctttttcaat attattgaag
     1201 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     1261 acaaataggc atgcagcgct cttccgcttc ctcgctcact gactcgctac gctcggtcgt
     1321 tcgactgcgg cgagcggtgt cagctcactc aaaagcggta atacggttat ccacagaatc
     1381 aggggataaa gccggaaaga acatgtgagc aaaaagcaaa gcaccggaag aagccaacgc
     1441 cgcaggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1501 gccagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1561 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1621 cttcgggaag cgtggcgctt tctcatagct cacgctgttg gtatctcagt tcggtgtagg
     1681 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1741 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1801 cagccattgg taactgattt agaggacttt gtcttgaagt tatgcacctg ttaaggctaa
     1861 actgaaagaa cagattttgg tgagtgcggt cctccaaccc acttaccttg gttcaaagag
     1921 ttggtagctc agcgaacctt gagaaaacca ccgttggtag cggtggtttt tctttattta
     1981 tgagatgatg aatcaatcgg tctatcaagt caacgaacag ctattccgtt actctagatt
     2041 tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg
     2101 taattctcat gttagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc
     2161 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt
     2221 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     2281 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc
     2341 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag
     2401 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg
     2461 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg
     2521 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc
     2581 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac
     2641 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat
     2701 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc
     2761 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca
     2821 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga
     2881 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg
     2941 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg
     3001 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta
     3061 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc
     3121 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc
     3181 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc
     3241 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact
     3301 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga
     3361 aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg
     3421 cattaggaaa ttaatacgac tcactata
//
