LOCUS       pSCL27                  6288 bp    DNA     circular UNA 08-APR-2022
DEFINITION  Eco1 WT.
ACCESSION   urn.local...24-ckph90c
VERSION     urn.local...24-ckph90c
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        127..231
                     /standard_name="AmpR promoter"
     CDS             232..300
                     /codon_start=1
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA"
                     /standard_name="AmpR"
     CDS             301..1092
                     /codon_start=1
                     /transl_table=1
                     /translation="HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMM
                     STFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAI
                     TMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVA
                     MATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSR
                     GIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /standard_name="AmpR"
     rep_origin      1263..1851
     promoter        1907..2267
                     /standard_name="pGal7"
     gene            2274..3236
                     /standard_name="ec86RT (mOpt)"
     misc_feature    2276^2277
                     /standard_name="AGCAGGGCCGACCCTAAGAAGAAGCGAAAAGTT"
                     /note="Geneious type: Editing History Deletion"
     misc_RNA        3247..3327
                     /standard_name="msdRNA"
     IR              3247..3258
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="inverted repeat"
                     /modified_by="katecrawford"
                     /label="a1"
     misc_feature    complement(3317..3402)
                     /standard_name="msDNA"
     motif           3317..3334
                     /standard_name="Flanking"
     motif           3335..3357
                     /standard_name="Stem frag"
     motif           3358..3363
                     /standard_name="Loop"
     motif           complement(3364..3386)
                     /standard_name="Stem frag"
     motif           complement(3387..3402)
                     /created_by="katecrawford"
                     /label="Flanking"
     misc_difference 3394
                     /standard_name="T-->A"
     IR              complement(3404..3415)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="inverted repeat"
                     /modified_by="katecrawford"
                     /label="a2"
     terminator      3439..3797
                     /standard_name="tGal7"
     promoter        complement(3811..3829)
                     /standard_name="T7_promoter"
     misc_feature    3841..3855
                     /standard_name="lacZ_a"
     CDS             3856
                     /codon_start=1
                     /transl_table=1
                     /standard_name="lacZα-Broken"
     CDS             3857..4217
                     /codon_start=1
                     /transl_table=1
                     /translation="TS*LGKPWRYPT*SPCSTSPFRQLA**RRGPHRSPFPTVAQPEW
                     RMARRAL*RRIKRGGCGGYAQRDRYTCQRPSARSFRFLPFLSRHVRRLSPSSSKSGAP
                     FRVPI*CFTAPRPQKT*L"
                     /standard_name="lacZα"
     rep_origin      4000..4455
     CDS             complement(4586..5389)
                     /codon_start=1
                     /transl_table=1
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
                     /standard_name="URA3"
     promoter        complement(5390..5610)
                     /standard_name="URA3 promoter"
     misc_feature    5875..90
                     /standard_name="CEN/ARS"
ORIGIN      
        1 ttttctttaa tttctttttt tactttctat ttttaattta tatatttata ttaaaaaatt
       61 taaattataa ttatttttat agcacgtgat gaaaaggacc caggtggcac ttttcgggga
      121 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      181 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      241 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      301 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
      361 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
      421 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
      481 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
      541 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
      601 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
      661 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
      721 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
      781 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
      841 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
      901 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
      961 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     1021 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1081 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1141 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     1201 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1261 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1321 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1381 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     1441 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     1501 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     1561 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     1621 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     1681 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     1741 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     1801 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     1861 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcaagt agagggggta
     1921 atttttcccc tttattttgt tcatacattc ttaaattgct ttgcctctcc ttttggaaag
     1981 ctatacttcg gagcactgtt gagcgaaggc tcattagata tattttctgt cattttcctt
     2041 aacccaaaaa taagggaaag ggtccaaaaa gcgctcggac aactgttgac cgtgatccga
     2101 aggactggct atacagtgtt cacaaaatag ccaagctgaa aataatgtgt agctatgttc
     2161 agttagtttg gctagcaaag atataaaagc aggtcggaaa tatttatggg cattattatg
     2221 cagagcatca acatgataaa aaaaaacagt tgaatattcc ctcaaaacgc cacatgaaat
     2281 ctgcagagta tctgaatacg ttccgcctta ggaatttggg cctccccgtg atgaacaatc
     2341 tccacgatat gagcaaggcg actcgaatat ccgtggaaac gctgagactg ctcatctata
     2401 cagcagactt tcggtacagg atctacacgg tcgaaaagaa ggggcctgag aaacgcatgc
     2461 gaacaattta tcaacctagc cgagagctca aggcgttgca gggctgggtt cttcgaaaca
     2521 tccttgacaa actctcatca tcacccttta gtattgggtt tgaaaagcac caaagcatcc
     2581 ttaacaacgc gacgccacac ataggtgcca atttcatatt gaacatcgac ttggaggatt
     2641 tttttccgag cctcacagcc aataaagtgt tcggtgtttt tcacagtctt gggtacaatc
     2701 gccttattag ttccgttctt accaagattt gttgttacaa gaatctcttg ccccagggag
     2761 cacccagcag tccgaaattg gcgaatttga tttgttccaa gctcgattat cgaatacaag
     2821 ggtacgcggg cagccgggga ctcatctata cccgctacgc agacgatctt acgctgtctg
     2881 cccaatcaat gaagaaggtc gtaaaggcgc gggatttctt gttttctatc atcccgtccg
     2941 agggcttggt aattaattcc aaaaagactt gtatctcagg accacgatct cagcgaaaag
     3001 tgacaggact cgtcatttct caagaaaaag tcggtatagg gagagagaag tataaggaaa
     3061 tccgcgcgaa gatccaccac atattctgtg gcaagagcag cgagatagaa cacgtccgag
     3121 gctggttgtc cttcatactg agcgtggact caaaaagcca ccgccggttg atcacctata
     3181 tttcaaaact ggaaaagaaa tatggaaaga acccactcaa caaagctaaa acatagaccg
     3241 gttagatgcg cacccttagc gagaggttta tcattaaggt caacctctgg atgttgtttc
     3301 ggcatcctgc attgaatctg agttactgtc tgttttcctt gttggaacgg agagcatcgc
     3361 ctgatgctct ccgagccaac caggaaaccc gttatttctg acgtaagggt gcgcaaccgg
     3421 ttagtaatga gtttaaacaa agaaagtgga atattcattc atatcatatt ttttctatta
     3481 actgcctggt ttcttttaaa ttttttattg gttgtcgact tgaacggagt gacaatatat
     3541 atatatatat atttaataat gacatcatta tctgtaaatc tgattcttaa tgctattcta
     3601 gttatgtaag agtggtcctt tccataaaaa aaaaaaaaaa gaaaaaagaa ttttaggaat
     3661 acaatgcagc ttgtaagtaa aatctggaat attcatatcg ccacaacttc ttatgcttat
     3721 aaaagcacta atgcctgaat ttatgttgaa aatatgtgtc acaaataaag aaactgtgac
     3781 atctgacaca tttccacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     3841 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     3901 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     3961 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt agcggcgcat
     4021 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4081 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4141 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4201 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4261 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4321 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     4381 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     4441 taacgtttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     4501 ccgcataggg taataactga tataattaaa ttgaagctct aatttgtgag tttagtatac
     4561 atgcatttac ttataataca gttttttagt tttgctggcc gcatcttctc aaatatgctt
     4621 cccagcctgc ttttctgtaa cgttcaccct ctaccttagc atcccttccc tttgcaaata
     4681 gtcctcttcc aacaataata atgtcagatc ctgtagagac cacatcatcc acggttctat
     4741 actgttgacc caatgcgtct cccttgtcat ctaaacccac accgggtgtc ataatcaacc
     4801 aatcgtaacc ttcatctctt ccacccatgt ctctttgagc aataaagccg ataacaaaat
     4861 ctttgtcgct cttcgcaatg tcaacagtac ccttagtata ttctccagta gatagggagc
     4921 ccttgcatga caattctgct aacatcaaaa ggcctctagg ttcctttgtt acttcttctg
     4981 ccgcctgctt caaaccgcta acaatacctg ggcccaccac accgtgtgca ttcgtaatgt
     5041 ctgcccattc tgctattctg tatacacccg cagagtactg caatttgact gtattaccaa
     5101 tgtcagcaaa ttttctgtct tcgaagagta aaaaattgta cttggcggat aatgccttta
     5161 gcggcttaac tgtgccctcc atggaaaaat cagtcaagat atccacatgt gtttttagta
     5221 aacaaatttt gggacctaat gcttcaacta actccagtaa ttccttggtg gtacgaacat
     5281 ccaatgaagc acacaagttt gtttgctttt cgtgcatgat attaaatagc ttggcagcaa
     5341 caggactagg atgagtagca gcacgttcct tatatgtagc tttcgacatg atttatcttc
     5401 gtttcctgca ggtttttgtt ctgtgcagtt gggttaagaa tactgggcaa tttcatgttt
     5461 cttcaacact acatatgcgt atatatacca atctaagtct gtgctccttc cttcgttctt
     5521 ccttctgttc ggagattacc gaatcaaaaa aatttcaaag aaaccgaaat caaaaaaaag
     5581 aataaaaaaa aaatgatgaa ttgaattgaa aagctgtggt atggtgcact ctcagtacaa
     5641 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     5701 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     5761 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     5821 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag gacggatcgc
     5881 ttgcctgtaa cttacacgcg cctcgtatct tttaatgatg gaataatttg ggaatttact
     5941 ctgtgtttat ttatttttat gttttgtatt tggattttag aaagtaaata aagaaggtag
     6001 aagagttacg gaatgaagaa aaaaaaataa acaaaggttt aaaaaatttc aacaaaaagc
     6061 gtactttaca tatatattta ttagacaaga aaagcagatt aaatagatat acattcgatt
     6121 aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac acagacaaga
     6181 tgaaacaatt cggcattaat acctgagagc aggaagagca agataaaagg tagtatttgt
     6241 tggcgatccc cctagagtct tttacatctt cggaaaacaa aaactatt
//
