LOCUS       pSCL28                  6377 bp    DNA     circular UNA 08-APR-2022
DEFINITION  Eco1 long primer.
ACCESSION   urn.local...2o-ckphag8
VERSION     urn.local...2o-ckphag8
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        127..231
                     /standard_name="AmpR promoter"
     CDS             232..300
                     /codon_start=1
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA"
                     /standard_name="AmpR"
     CDS             301..1092
                     /codon_start=1
                     /transl_table=1
                     /translation="HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMM
                     STFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAI
                     TMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVA
                     MATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSR
                     GIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /standard_name="AmpR"
     rep_origin      1263..1851
     promoter        1907..2267
                     /standard_name="pGal7"
     gene            2274..3236
                     /standard_name="ec86RT (mOpt)"
     extention       3306..3320
                     /standard_name="extended primer"
                     /modified_by="katecrawford"
                     /label="extended a1"
     misc_RNA        3320..3401
                     /standard_name="msdRNA"
     extention       3321..3332
                     /standard_name="primer"
                     /modified_by="katecrawford"
                     /label="a1"
     misc_feature    complement(3391..3476)
                     /standard_name="msDNA"
     motif           3391..3408
                     /standard_name="Flanking"
     motif           3409..3431
                     /standard_name="Stem frag"
     motif           3432..3437
                     /standard_name="Loop"
     motif           complement(3438..3460)
                     /standard_name="Stem frag"
     motif           complement(3461..3476)
                     /created_by="katecrawford"
                     /modified_by="katecrawford"
                     /label="flanking"
     misc_difference 3468
                     /standard_name="T-->A"
     extention       complement(3478..3489)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="primer"
                     /modified_by="katecrawford"
                     /label="a2"
     extention       complement(3490..3504)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="long primer"
                     /modified_by="katecrawford"
                     /label="extended a2"
     terminator      3528..3886
                     /standard_name="tGal7"
     promoter        complement(3900..3918)
                     /standard_name="T7_promoter"
     misc_feature    3930..3944
                     /standard_name="lacZ_a"
     CDS             3945
                     /codon_start=1
                     /transl_table=1
                     /standard_name="lacZα-Broken"
     CDS             3946..4306
                     /codon_start=1
                     /transl_table=1
                     /translation="TS*LGKPWRYPT*SPCSTSPFRQLA**RRGPHRSPFPTVAQPEW
                     RMARRAL*RRIKRGGCGGYAQRDRYTCQRPSARSFRFLPFLSRHVRRLSPSSSKSGAP
                     FRVPI*CFTAPRPQKT*L"
                     /standard_name="lacZα"
     rep_origin      4089..4544
     CDS             complement(4675..5478)
                     /codon_start=1
                     /transl_table=1
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
                     /standard_name="URA3"
     promoter        complement(5479..5699)
                     /standard_name="URA3 promoter"
     misc_feature    5964..90
                     /standard_name="CEN/ARS"
ORIGIN      
        1 ttttctttaa tttctttttt tactttctat ttttaattta tatatttata ttaaaaaatt
       61 taaattataa ttatttttat agcacgtgat gaaaaggacc caggtggcac ttttcgggga
      121 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      181 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      241 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      301 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
      361 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
      421 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
      481 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
      541 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
      601 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
      661 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
      721 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
      781 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
      841 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
      901 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
      961 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     1021 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1081 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1141 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     1201 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1261 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1321 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1381 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     1441 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     1501 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     1561 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     1621 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     1681 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     1741 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     1801 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     1861 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcaagt agagggggta
     1921 atttttcccc tttattttgt tcatacattc ttaaattgct ttgcctctcc ttttggaaag
     1981 ctatacttcg gagcactgtt gagcgaaggc tcattagata tattttctgt cattttcctt
     2041 aacccaaaaa taagggaaag ggtccaaaaa gcgctcggac aactgttgac cgtgatccga
     2101 aggactggct atacagtgtt cacaaaatag ccaagctgaa aataatgtgt agctatgttc
     2161 agttagtttg gctagcaaag atataaaagc aggtcggaaa tatttatggg cattattatg
     2221 cagagcatca acatgataaa aaaaaacagt tgaatattcc ctcaaaacgc cacatgaaat
     2281 ctgcagagta tctgaatacg ttccgcctta ggaatttggg cctccccgtg atgaacaatc
     2341 tccacgatat gagcaaggcg actcgaatat ccgtggaaac gctgagactg ctcatctata
     2401 cagcagactt tcggtacagg atctacacgg tcgaaaagaa ggggcctgag aaacgcatgc
     2461 gaacaattta tcaacctagc cgagagctca aggcgttgca gggctgggtt cttcgaaaca
     2521 tccttgacaa actctcatca tcacccttta gtattgggtt tgaaaagcac caaagcatcc
     2581 ttaacaacgc gacgccacac ataggtgcca atttcatatt gaacatcgac ttggaggatt
     2641 tttttccgag cctcacagcc aataaagtgt tcggtgtttt tcacagtctt gggtacaatc
     2701 gccttattag ttccgttctt accaagattt gttgttacaa gaatctcttg ccccagggag
     2761 cacccagcag tccgaaattg gcgaatttga tttgttccaa gctcgattat cgaatacaag
     2821 ggtacgcggg cagccgggga ctcatctata cccgctacgc agacgatctt acgctgtctg
     2881 cccaatcaat gaagaaggtc gtaaaggcgc gggatttctt gttttctatc atcccgtccg
     2941 agggcttggt aattaattcc aaaaagactt gtatctcagg accacgatct cagcgaaaag
     3001 tgacaggact cgtcatttct caagaaaaag tcggtatagg gagagagaag tataaggaaa
     3061 tccgcgcgaa gatccaccac atattctgtg gcaagagcag cgagatagaa cacgtccgag
     3121 gctggttgtc cttcatactg agcgtggact caaaaagcca ccgccggttg atcacctata
     3181 tttcaaaact ggaaaagaaa tatggaaaga acccactcaa caaagctaaa acatagaccg
     3241 gttagcgtga aatgtctttg gatttgggaa tcttataagt tctgtatgag accaccattt
     3301 aactatgata agattccgta tgcgcaccct tagcgagagg tttatcatta aggtcaacct
     3361 ctggatgttg tttcggcatc ctgcattgaa tctgagttac tgtctgtttt ccttgttgga
     3421 acggagagca tcgcctgatg ctctccgagc caaccaggaa acccgttatt tctgacgtaa
     3481 gggtgcgcat acggaatctt atcaaccggt tagtaatgag tttaaacaaa gaaagtggaa
     3541 tattcattca tatcatattt tttctattaa ctgcctggtt tcttttaaat tttttattgg
     3601 ttgtcgactt gaacggagtg acaatatata tatatatata tttaataatg acatcattat
     3661 ctgtaaatct gattcttaat gctattctag ttatgtaaga gtggtccttt ccataaaaaa
     3721 aaaaaaaaag aaaaaagaat tttaggaata caatgcagct tgtaagtaaa atctggaata
     3781 ttcatatcgc cacaacttct tatgcttata aaagcactaa tgcctgaatt tatgttgaaa
     3841 atatgtgtca caaataaaga aactgtgaca tctgacacat ttccacgtac ccaattcgcc
     3901 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     3961 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     4021 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     4081 atggcgcgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4141 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4201 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4261 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4321 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4381 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4441 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4501 aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcctgat gcggtatttt
     4561 ctccttacgc atctgtgcgg tatttcacac cgcatagggt aataactgat ataattaaat
     4621 tgaagctcta atttgtgagt ttagtataca tgcatttact tataatacag ttttttagtt
     4681 ttgctggccg catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc
     4741 taccttagca tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc
     4801 tgtagagacc acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc
     4861 taaacccaca ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc
     4921 tctttgagca ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc
     4981 cttagtatat tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag
     5041 gcctctaggt tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg
     5101 gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc
     5161 agagtactgc aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa
     5221 aaaattgtac ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc
     5281 agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa
     5341 ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc
     5401 gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt
     5461 atatgtagct ttcgacatga tttatcttcg tttcctgcag gtttttgttc tgtgcagttg
     5521 ggttaagaat actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa
     5581 tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa
     5641 atttcaaaga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaattgaaa
     5701 agctgtggta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     5761 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     5821 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     5881 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     5941 gataataatg gtttcttagg acggatcgct tgcctgtaac ttacacgcgc ctcgtatctt
     6001 ttaatgatgg aataatttgg gaatttactc tgtgtttatt tatttttatg ttttgtattt
     6061 ggattttaga aagtaaataa agaaggtaga agagttacgg aatgaagaaa aaaaaataaa
     6121 caaaggttta aaaaatttca acaaaaagcg tactttacat atatatttat tagacaagaa
     6181 aagcagatta aatagatata cattcgatta acgataagta aaatgtaaaa tcacaggatt
     6241 ttcgtgtgtg gtcttctaca cagacaagat gaaacaattc ggcattaata cctgagagca
     6301 ggaagagcaa gataaaaggt agtatttgtt ggcgatcccc ctagagtctt ttacatcttc
     6361 ggaaaacaaa aactatt
//
